SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene25512

Length: 2172 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene25512
ATGGGGACGCCTCTCAATTAATATCTGGACTCGAAGACCAATTGCTCACCCAGCGATTCGATTCGATTCGATACTGGCAATCATGTGTCCTCCAAGCAGAAACAGGAGGGAGCGGCCATCAGAAATAAAATGGTGGACGCAGGAACCGTGGAAGGTGAAGGAGGCCTTCAGCGTATTGGACAAAGACGGCGACGGAAAAATCAGTTTGTTGGATCTCCAGCATTTCTTCAGCTTGGGGAAGGGGCTGAGCAGAGAGGAAATGGAGGCCATGATATCTGTTGCCGACGCCGACAACAGCGGCTCTGTGGATTTTGAGGAGTTTCGCCGGATCTTGCAGCTGAATATGCCGGAAATTCAAGAAATCAAAGACAGCAGATCGAATTCTGATGATGACGGTGAGATGTGGGCGTTGAAGGAGGCGTTTAATGTGATGGACAGAGACGGAGACGGGATTGTGAGCGCAGAGGATCTCAAGGCGTTCCTCTCCTCCTCTATCGCTACAGGCGGTGGCAGCGGTGAAAGCTGCAATGTAATAATGAGCCACGAGGATTTGGCGGAGATGATAGAGGCGACAGGCGGGTCCGCCAAATGTGGCGTTTGCTACGAAGATTTTGTCCGAATGATGATAACGACGATGAATGGCTGAGTGGTCTATAATGTGATTTTTTTGGAGATTCAGGTGACGGAATCCGGCGGAAGAAATTAAGATTAAATCTTTAATTGTTGTAGTTAAAATGGTTTTTTAATTATATATTTAGATTCAGCACCCTGAAAAACCCCTGGCACGCTTTGATTGTGCAGAAACTAGCTATCAAACTTGTGATTCCAATAAATTGTTGCCGGATTTTGACCAGAAAAATCGGCTGGAAAAGCTTTCAAAAAAACAACTGCCTATTAATTTGGCTGTTTCGTTAGGCGTTGTCTACTGAAGTCTCAATTTTCCGATAGAAAGTAATTATTTATGCAGTTGTCTATAACGCGACTTTTTTGAAAACTTTTCCGGCTGGTTTTTCTGGTCAAAATCACGCAGCAATTTATTGGAATTGCAAGTTTGATAGCTAGTTTCTGCACAATCAAAGCGTCCCAGGGGTTTTTCAGGGTGCTGAATCCAAATATGCAGTTAAAAATCCATTTTAACTGCAAAAAATCAAGCTTTAACCTCAATTTCTTCCGCCGGATTCAGTCACCAAAATCTCCAAAAAAATGGCATTATAAACAACTCTGAAACCCTATACAAGTAATCGGCCAGGTATCCAATATTTTAGGGGCACTATCAATTTGATAACTTAAGTTGAACCTCCTGTGTTGGAACCTGGGAGATTGAACAAATATAACTGAGAAATCTTGAGATGATCAGCGAGATACAAATGACTCTACCCTCTGTTGTGCCCTCTGATAGGATCAATTGTAACTCTAGTGAATACAGATCCCAACCAGTGGAAAGATTCAATCATGTATTTAAATAGTGGTTTGGAAGATGGCCGAGGCAATAGGAAGAAAATTGAAGGGGCGTTTGTCACAATTACTTCTACTAGTAAAAAGAATTAAGGAGTAGGTATTGCACTAAATCTTATCAGCACACCGATATACCTCCGCAACACGACAAACGACCTCATCAACAACATTATTGTCATTTTGAAAGCGACCTACACGATGGCGGCCGCCACGGCGTGATGAATTTAGTAATTGCCTTTTCTGCAGACACGAGCGTTCTTCCATACTCTGCCTGGCCTGCACAAGTAACCCACCTGTGGAGCGCAATGGACATCCTCTCTACACTTGCACAGTTTCTCCAGCGCACACCCATCTTTCAGGACCTCAACCTTTTGGCCCCCAATTCCGAGAAGTCCATCAGTCCATTCGTTAGAGAAGAGGCCATCGGGATCGAGCCTCTTTTTCACCGTCAAAAACTGCTGGCAAATTCACGGTTTTCTCAGCCAATCCTTGGAACAAATGACCCCCAGACTTCCCCCAGTGCAGAGTCCCTCCATGATTTTCTATCAGCATCTGCTCTATCTCTTGATAGACGTCCATGTCCCATGTCGGAACGTAGGCCTGCCGAGACCGAGAGTAAATTATCTCAAATGTCACAACGTCTTCGGCAGGGCCCAAATAGGCCGCCTCCGATTTCTTAATCGTTCGCATAATTGAGTGACAGCCAAGGTCACGACG

Ace file of the UniGene sp_v1.1_unigene25512

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[TZDR] COG5126 Ca2+-binding protein (EF-Hand superfamily)	-	-	3.0e-12	32%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	71%
Blast2go	fad-binding domain-containing protein	-	-	0.0	78%

Source	InterPros	Term	Type	Identity
Blast2go	Calcium-binding EF-hand	IPR002048	-	78%
Blast2go	EF-hand-like domain	IPR011992	-	78%
Blast2go	EF-Hand 1, calcium-binding site	IPR018247	-	78%
Blast2go	EF-hand	IPR018248	-	78%
Blast2go	EF-HAND 2	IPR018249	-	78%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NZM5

Fln msg:

Fln protein: MCPPSRNRRERPSEIKWWTQEPWKVKEAFSVLDKDGDGKISLLDLQHFFSLGKGLSREEMEAMISVADADNSGSVDFEEFRRILQLNMPEIQEIKDSRSNSDDDGEMWALKEAFNVMDRDGDGIVSAEDLKAFLSSSIATGGGSGESCNVIMSHEDLAEMIEATGGSAKCGVCYEDFVRMMITTMNG*

Protein Length: 189

Fln nts: ATGGGGACGCCTCTCAATTAATATCTGGACTCGAAGACCAATTGCTCACCCAGCGATTCGATTCGATTCGATACTGGCAATC_-_ATGTGTCCTCCAAGCAGAAACAGGAGGGAGCGGCCATCAGAAATAAAATGGTGGACGCAGGAACCGTGGAAGGTGAAGGAGGCCTTCAGCGTATTGGACAAAGACGGCGACGGAAAAATCAGTTTGTTGGATCTCCAGCATTTCTTCAGCTTGGGGAAGGGGCTGAGCAGAGAGGAAATGGAGGCCATGATATCTGTTGCCGACGCCGACAACAGCGGCTCTGTGGATTTTGAGGAGTTTCGCCGGATCTTGCAGCTGAATATGCCGGAAATTCAAGAAATCAAAGACAGCAGATCGAATTCTGATGATGACGGTGAGATGTGGGCGTTGAAGGAGGCGTTTAATGTGATGGACAGAGACGGAGACGGGATTGTGAGCGCAGAGGATCTCAAGGCGTTCCTCTCCTCCTCTATCGCTACAGGCGGTGGCAGCGGTGAAAGCTGCAATGTAATAATGAGCCACGAGGATTTGGCGGAGATGATAGAGGCGACAGGCGGGTCCGCCAAATGTGGCGTTTGCTACGAAGATTTTGTCCGAATGATGATAACGACGATGAATGGCTGA___GTGGTCTATAATGTGATTTTTTTGGAGATTCAGGTGACGGAATCCGGCGGAAGAAATTAAGATTAAATCTTTAATTGTTGTAGTTAAAATGGTTTTTTAATTATATATTTAGATTCAGCACCCTGAAAAACCCCTGGCACGCTTTGATTGTGCAGAAACTAGCTATCAAACTTGTGATTCCAATAAATTGTTGCCGGATTTTGACCAGAAAAATCGGCTGGAAAAGCTTTCAAAAAAACAACTGCCTATTAATTTGGCTGTTTCGTTAGGCGTTGTCTACTGAAGTCTCAATTTTCCGATAGAAAGTAATTATTTATGCAGTTGTCTATAACGCGACTTTTTTGAAAACTTTTCCGGCTGGTTTTTCTGGTCAAAATCACGCAGCAATTTATTGGAATTGCAAGTTTGATAGCTAGTTTCTGCACAATCAAAGCGTCCCAGGGGTTTTTCAGGGTGCTGAATCCAAATATGCAGTTAAAAATCCATTTTAACTGCAAAAAATCAAGCTTTAACCTCAATTTCTTCCGCCGGATTCAGTCACCAAAATCTCCAAAAAAATGGCATTATAAACAACTCTGAAACCCTATACAAGTAATCGGCCAGGTATCCAATATTTTAGGGGCACTATCAATTTGATAACTTAAGTTGAACCTCCTGTGTTGGAACCTGGGAGATTGAACAAATATAACTGAGAAATCTTGAGATGATCAGCGAGATACAAATGACTCTACCCTCTGTTGTGCCCTCTGATAGGATCAATTGTAACTCTAGTGAATACAGATCCCAACCAGTGGAAAGATTCAATCATGTATTTAAATAGTGGTTTGGAAGATGGCCGAGGCAATAGGAAGAAAATTGAAGGGGCGTTTGTCACAATTACTTCTACTAGTAAAAAGAATTAAGGAGTAGGTATTGCACTAAATCTTATCAGCACACCGATATACCTCCGCAACACGACAAACGACCTCATCAACAACATTATTGTCATTTTGAAAGCGACCTACACGATGGCGGCCGCCACGGCGTGATGAATTTAGTAATTGCCTTTTCTGCAGACACGAGCGTTCTTCCATACTCTGCCTGGCCTGCACAAGTAACCCACCTGTGGAGCGCAATGGACATCCTCTCTACACTTGCACAGTTTCTCCAGCGCACACCCATCTTTCAGGACCTCAACCTTTTGGCCCCCAATTCCGAGAAGTCCATCAGTCCATTCGTTAGAGAAGAGGCCATCGGGATCGAGCCTCTTTTTCACCGTCAAAAACTGCTGGCAAATTCACGGTTTTCTCAGCCAATCCTTGGAACAAATGACCCCCAGACTTCCCCCAGTGCAGAGTCCCTCCATGATTTTCTATCAGCATCTGCTCTATCTCTTGATAGACGTCCATGTCCCATGTCGGAACGTAGGCCTGCCGAGACCGAGAGTAAATTATCTCAAATGTCACAACGTCTTCGGCAGGGCCCAAATAGGCCGCCTCCGATTTCTTAATCGTTCGCATAATTGAGTGACAGCCAAGGTCACGACG

Fln Alignment:

sp_v1.1_unigene25512___IKWWTQEPWKVKEAFSVLDKDGDGKISLLDLQHFFS-----LGKGLSREEMEAMISVADADNSGSVDFEEFRRILQLNMPEIQEIKDSRSNSDDDGEMWALKEAFNVMDRDGDGIVSAEDLKAFLSSSIATGGGSGESCNVIMSHEDLAEMIEATGGSAKCGVCYEDFVRMMITTMNG
A9NZM5_______________IKSWTEEPWKVKEAFNLLDKDGDGKIGLSDLQDFFSSSRFNVGKGLSREEMESMISVADTDNSGSVDFEEFHRILRLIMPEIDETEKRRSNTDD-AQMWALKEAFNVIDTDGDGIVSAEDLKTFFSSITASASSSSEICNAMMSEEDLGDMIEAAGGSAKCGVCYEGFARLMMTMMNG

SNPs (tot: 50)

Position	A %	C %	G %	T %	Probability
83	85			14	0.993533
84		14		85	0.993533
88	12			87	0.992554
94	87			12	0.992554
95	87		12		0.992554
98	87			12	0.992554
100	87			12	0.992554
101	87	12			0.992554
105			87	12	0.992554
106		12	87		0.992554
140		12	87		0.992554
239	90	10			0.90298
240	90			10	0.90298
242			90	10	0.90298
243		10	90		0.90298
244	10		90		0.90298
247	10		90		0.90298
377		66		33	1.0
463	85	10	5		0.999793
465	83	11		5	0.999887
474	88			11	0.999952
508		11		88	0.999952
511		88		11	0.999952
542		85	14		1.0
947	75		25		0.996473
1732		75		25	0.996473
1957		83	16		0.994512
1958		83		16	0.994512
1959		83	16		0.994512
1960	16	83			0.994512
1964	83	16			0.994512
1966	16			83	0.994512
1967	16			83	0.994512
1968		83	16		0.994512
1969		83	16		0.994512
1970		83		16	0.994512
1972	16	83			0.994512
1973	83	16			0.994512
1975	16			83	0.994512
1994		20		80	0.995493
2036		80	20		0.995493
2037	20	80			0.995493
2038		80	20		0.995493
2042	20			80	0.995493
2044	20		80		0.995493
2046	80	20			0.995493
2049	20		80		0.995493
2054		80		20	0.995493
2056	20			80	0.995493
2059	20	80			0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
54	72	CGATT CGATT CGATT CGAT	19

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain