SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene25257

Length: 1425 nt

Origin of reads: Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene25257 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Calcium binding protein n=1 Tax=Pinus taeda RepID=O22429_PINTA	-	-	93%
FL-Next	tr=Calcium binding protein; Pinus taeda (Loblolly pine).	-	-	93%
Blast2go	calmodulin-like protein 12	-	-	54%

Source	GOs	Term	Type	Identity
Blast2go	response to mechanical stimulus	GO:0009612	Biological Process	54%
Blast2go	response to temperature stimulus	GO:0009266	Biological Process	54%
Blast2go	binding	GO:0005488	Molecular Function	54%
Blast2go	cytoplasm	GO:0005737	Cellular Component	54%
Blast2go	intracellular membrane-bounded organelle	GO:0043231	Cellular Component	54%

Source	InterPros	Term	Type	Identity
Blast2go	Calcium-binding EF-hand	IPR002048	-	54%
Blast2go	EF-hand-like domain	IPR011992	-	54%
Blast2go	EF-Hand 1, calcium-binding site	IPR018247	-	54%
Blast2go	EF-hand	IPR018248	-	54%
Blast2go	EF-HAND 2	IPR018249	-	54%

Full-Lengther Next Prediction

Fln status: Misassembled

Fln database: coniferopsida.fasta

Fln subject: O22429

Fln msg: Coding sequence with some errors, ERROR#1, contains sense and antisense hits!!!, putative chimeric sequence,

Fln nts: TTCAAAACTGAAAGTACATGCATTCAACCGTTCAGCCATCATATCATGATAGAGTTAAGCGCTGAGAGCTGAGGTCGGGGAATATTTATTTATTTATTAATTACGGGGCGGCGTGGGCATCAATTAGCTGAAGCGAGGCCCAGCATGAGACGGGCGGCGTTGAGGCCGTTGCTGTTGGCGGCCACGAACTCGTCGAAGCTGATGAAGCAGTCTTTGTTGGAGTCCACGTCCTTCATCACACCATCAATCTCCTCCTCACTAGAGCTGCACTTGAGGATGGCGCTCAGTTCTGATTTCGATATTTTGCCGTCGCCGTTGGTGTCGAACTTGCGGAACACGTTCTCCAGCTCCAAGCGGCCGGGCAGAGCGGCACATTGCACAGCGGAAGCGGAATTTCGCATGAGACTGGCGGCCTTGAGGTTCTTGGAGTTGGCCTCCACCAACTCGTCGAAGTTGATGAATCCGTCTTTGTTGGAGTCCACCTCCTTCATCACCCCTTCAATCTCCGCCTCCGAAATCAGGGTAGTCTTTTCCTATTTGCTTCCCTTCACCATCACCTAGTTCAGCTGCGAGA_-_ATGGCTTCAGCTGTGAGCAGCCGCCTCGAGCTTGAGGAGGTGTTCCGCAAGTTCGACACCAACGGCGACGGCAAAATATCCAAGTCGGAACTGAGTGCCCTGATTTCGGAGGCGGAGATTGAAGGGGTGATGAAGGAGGTGGACTCCAACAAAGACGGATTCATCAACTTCGACGAGTTGGTGGAGGCCAACTCCAAGAACCTCAAGGCCGCCAGTCTCATGCGAAATTCCGCTTCCGCTGTGCAATGTGCCGCTCTGCCCGGCCGCTTGGAGCTGGAGAACGTGTTCCGCAAGTTCGACACCAACGGCGACGGCAAAATATCCAAGTCGGAACTGAGTGCCCTGATTTCGGAGGCGGAGATTGAAGGGGTGATGAAGGAGGTGGACTCCAACAAAGACGGATTCATCAACTTCGACGAGTTGGTGGAGGCCAACTCCAAGAACCTCAAGGCCGCCAGTCTCATGCGAAATTCCGCTTCCGCTGTGCAATGTGCCGCTCTGCCCGGCCGCTTGGAGCTGGAGAACGTGTTCCGCAAGTTCGACACCAACGGCGACGGCAAAATATCGAAATCGGAACTGAGCGCCATCCTCAAGTGCAGCTCTAGTGAGGAGGAGATTGATGGTGTGATGAAGGACGTGGACTCCAACAAAGACTGCTTCATCAGCTTCGACGAGTTCGTGGCCGCCAACAGCAACGGCCTCAACGCCGCCCGTCTCATGCTGGGCCTCGCTTCAGCTAATTGA___TGCCCACGCCGCCCCGTAATTAATAAATAAATAAATATTCCCCTACCTCAGCTCTCAGCGCTTAACTCTATCATGATATGATGGCTGAACGGTTGAGTGCGTGTACT

Fln Alignment:

Supercontig25257___RLELEEVFRKFDTNGDGKISKSELSALISEAEIEGVMKEVDSNKDGFINFDELVEANSKNLKAASLMRNSASAVQCAALPGRLELENVFRKFDTNGDGKISKSELSALI----SEAEIEGVMKEVDSNKDGFINFDELVEANSKNLKAASLMRNSASA
O22429_______________RRELEDVFRKFDTNGDGKISKSELSALISEAEIEGVMKEVDSNKDGFINFDELVEANSKNLNAASLMRNSASAVQCATLPGRLELEDVFRKFDTNGDGKISKSELSAILKCRSSEEEIDGVMKDVDSNKDGFISFDEFVAANSNGLNAARLMLGLASA

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
895	66			33	0.997452
896	33	66			0.997452
897	33			66	0.997452
898			66	33	0.997452
900		33	66		0.997452
902	33		66		0.997452
904		66	33		0.997452

SSRs (tot: 2)

Start position	End position	Sequence	Length
71	86	TATT TATT TATT TATT	16
1328	1343	AATA AATA AATA AATA	16

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain