SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene24727

Length: 1727 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene24727

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	LTM1; oligosaccharyl transferase STT3 protein-like; K07151 dolichyl-diphosphooligosaccharide--protein glycosyltransferase [EC:2.4.1.119]	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	protein	-	-	85%

Source	ECs	Term	Type	e value	Identity
AutoFact	Dolichyl-diphosphooligosaccharide--protein glycotransferase.	EC:2.4.1.119	-	0.0	77%

Source	KEGGs	Term	Identity
AutoFact	N-Glycan biosynthesis	00510	77%
AutoFact	Various types of N-glycan biosynthesis	00513	77%
AutoFact	Metabolic pathways	01100	77%

Source	GOs	Term	Type	Identity
Blast2go	response to salt stress	GO:0009651	Biological Process	85%
Blast2go	transferase activity	GO:0016740	Molecular Function	85%
Blast2go	membrane	GO:0016020	Cellular Component	85%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	85%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Oligosaccharyl transferase, STT3 subunit	IPR003674	-	0.0	85%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABH2

Fln msg: STOP codon was not found. Distance to subject end: 317 aas, your sequence is longer than subject: 486 - 169

Fln protein: MAASDKSRVGVRDAFGSVLSTIILILVAILAFSIRLFSVIKYESVIHEFDPYFNYRVTKYLTKSGVYDFWNWFDDRTWYPLGRVIGGTVYPGLTLTAGTMWWVFNSLNIPLSVETVCVFTAPIFSANAAWATYLLTKEVKGVGAGLTAAAILAMVPSYISRSVAGSYDNEAVAIFALIFTFYLYIKTLNTGSLFYATLNALAYFYMVCSWGGYTFIINLIPMHVLLCIVTGRYSSRLYIAYSPLVVLGTLLAALVPVVGFNAVMTSEHFASFLVFIIVQVVALGHYIKRTVSAKMFKVAITLVISVGMFVSIALVAILVALVAASPTKGWSGRSLSLLDPTYASKYIPIIASVSEHQPPTWPSYFMDINVLAFLVPAGIVACFLPLSDASSFLVLYIVTSVYFSGVMVRLMLVLAPAACILSGIALSEAFTVLTRSVKFQLSLSFEKDASTVKSETVPNELKDESKPTLLEKDKEPLRERPSKKNR

Protein Length: 487

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTTTAGAAATATAAACGGTGTGCCGAAAAAATATGGGAATCCTGTGCAGGGAGACCAAGGGAAGACTGGCAAAACAAAGAATAATCGAAACTAAAACCTTCGGAGTTCTTTCTTACGCTGTAAACCCTTCCTTGACTTTGCACCTTGATTATGGAGGGGTAGTAGAGAAGCAGAAAATGAGAGCAGAGCAGAACAGAGCGGGAATTTTTTCTTATTCTGCCAAATCAGTCTCTCCAAGGCC_-_ATGGCTGCCTCTGATAAATCGAGAGTTGGTGTTAGAGATGCATTCGGCTCCGTGTTATCGACGATCATCCTCATTTTAGTGGCCATTCTCGCGTTTTCCATCCGGCTTTTTTCGGTCATCAAGTATGAGAGCGTCATTCATGAGTTCGATCCTTACTTCAATTACAGAGTTACAAAGTATCTCACAAAGAGTGGAGTCTACGACTTTTGGAACTGGTTCGATGACCGAACCTGGTACCCACTGGGACGTGTCATTGGGGGAACAGTGTATCCTGGTCTCACTTTGACAGCAGGGACAATGTGGTGGGTATTTAATTCTCTCAACATCCCGCTTTCCGTAGAAACTGTTTGTGTCTTCACTGCTCCCATCTTCTCAGCAAATGCAGCTTGGGCAACATATCTTTTGACAAAGGAAGTTAAAGGTGTTGGTGCAGGCCTCACTGCAGCAGCCATTCTAGCAATGGTGCCTTCATACATCTCACGATCCGTGGCTGGCAGTTATGATAATGAGGCTGTAGCTATATTTGCTTTGATCTTTACATTCTATCTATATATCAAGACATTGAACACTGGATCTCTCTTTTATGCTACTTTAAATGCCTTAGCTTACTTTTATATGGTCTGTTCCTGGGGAGGTTACACTTTCATAATAAATCTTATTCCAATGCATGTTCTTCTATGTATTGTGACCGGCCGGTACTCATCTCGTCTTTACATTGCTTATTCACCTCTGGTAGTTTTGGGTACTTTGCTGGCTGCTCTTGTTCCTGTTGTTGGTTTCAACGCAGTCATGACATCTGAGCATTTTGCGTCATTCCTGGTGTTTATCATAGTTCAGGTTGTCGCGTTGGGGCATTATATAAAGCGTACTGTGTCAGCTAAGATGTTCAAAGTGGCAATAACTCTTGTAATTTCTGTTGGAATGTTTGTGTCTATTGCATTGGTAGCAATATTGGTTGCATTAGTGGCAGCAAGTCCCACAAAGGGATGGAGTGGACGAAGTCTAAGTCTTCTTGATCCGACCTATGCGAGCAAGTATATTCCTATTATTGCGAGTGTCAGTGAACATCAACCACCTACTTGGCCTTCATATTTTATGGATATAAATGTACTAGCATTTCTAGTCCCTGCAGGCATCGTTGCATGTTTCTTGCCATTGTCGGATGCAAGCTCCTTTTTGGTTCTCTACATTGTGACATCTGTGTACTTTTCTGGTGTAATGGTTCGGCTTATGCTAGTGCTGGCTCCAGCAGCTTGTATTTTATCTGGCATTGCTCTTTCTGAAGCTTTTACAGTACTAACACGATCAGTCAAATTTCAGCTCTCTCTCTCATTTGAAAAGGATGCTTCAACAGTCAAATCAGAAACAGTTCCAAATGAATTGAAGGACGAGTCCAAACCAACACTCCTTGAGAAAGATAAAGAACCTTTAAGAGAGAGACCCTCTAAAAAGAACAGGT

Fln Alignment:

sp_v1.1_unigene24727___MAASDKSRVGVRDAFGSVLSTIILILVAILAFSIRLFSVIKYESVIHEFDPYFNYRVTKYLTKSGVYDFWNWFDDRTWYPLGRVIGGTVYPGLTLTAGTMWWVFNSLNIPLSVETVCVFTAPIFSANAAWATYLLTKEVKGVGAGLTAAAILAMVPSYISRSVAGSYDN
D5ABH2_______________MAASDKSRVGVRDAFGSVLSTAILILVAILAFSIRLFSVIKYESVIHEFDPYFNYRVTKYLTKSGVYDFWNWFDDRTWYPLGRVIGGTVYPGLTLTAGTMWWVFNALNIPLSVETVCVFTAPIFSANAAWATYLLKKEVKGIGAGLTAATILAMVPSYISRSVAGSYDN

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
61	33		33	33	0.997753
62	33		33	33	0.997753
63	33		66		0.997452
64		33	66		0.997452
65	66		33		0.997452
260		14		85	0.993533
295		14		85	0.993533
1004	11		88		0.923564
1006			14	85	0.993533
1007		14		85	0.993533
1008	14			85	0.993533
1009		14	85		0.993533
1135		14		85	0.993533
1168	85		14		0.993533
1228	75		25		1.0
1251	90		10		0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain