SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene24013

Length: 1859 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene24013

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	acetylesterase, putative	-	-	44%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	70%
Blast2go	lanatoside 15-o-acetylesterase	-	-	66%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Lipase, GDSL	IPR001087	-	0.0	66%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LK87

Fln msg: Overlapping hits, possible frame ERROR between 1018 and 883,

Fln protein: MNSLLFKRCGACIVMIMTLLPAMCYSSCEYPAIFNFGDSTSDTGTIHYIFPYNMLAENPPYGRTFFGRPVNRYSDGRLSIDFFAQALGLPFLNPYLQSVAASFENGANFAAAGATVRETNFIAPISLTVQINQYKIFKQQVAATIQAHGNVSYLPTMDAFRTGIYVLEIGGNDFSFGYMNLHLNTSxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTHSSNDFDQYGCSLSYNNAVRLYNRMLRAQLAVTRKELPDATIIYVNTYDIVYDFFVNPSKYGFKFTTRACCGVGGKYNYNYAVQCGSTGTIKNTSVTAESCPDPASYVNWDGVHWTDEANKIITQQILSGKYFEPAFSIASHCNLQPL*

Protein Length: 384

Fln nts: CGTCGTGACCTTGGCTGTCACTCAACACAGGACCGTTTGGAGGGCGCGCCCTCTTTAACCGTTGAACGAATTCAATACGCGGTTTGTTGGTTTCACTTTTTCTTTAAATGCCAGCGGTGGTGCTAAATTTTCCACTCTCTTACCGTGAACTGTAAGTAAAATACAGCGGTGGTGCCAAAATTTCGAATCTCTTTCCAGCCATTGCCGTCGGCTGAAGATTTGTTAAAGCTTGGTTGCTTTTCTGGAATTCTCAACCTTATGACTGTAGAGATTTTTGTATAAATATTTACTCAATTCGAAGCTTATACCATACAGAGTCATC_-_ATGAATTCTCTTTTGTTTAAACGCTGTGGGGCTTGTATAGTTATGATTATGACATTGCTGCCTGCAATGTGTTATAGTAGCTGTGAATATCCAGCCATTTTTAACTTTGGGGACTCCACCTCTGATACGGGCACAATACATTACATTTTCCCTTACAATATGCTCGCGGAGAATCCTCCCTATGGCCGAACTTTCTTTGGAAGACCTGTAAATCGTTACAGCGATGGAAGACTGTCAATAGATTTTTTTGCGCAGGCATTGGGATTGCCATTCCTGAACCCATATTTACAGTCTGTGGCTGCGAGTTTCGAAAATGGAGCCAACTTTGCTGCTGCAGGTGCCACTGTACGTGAAACAAATTTCATTGCCCCCATTTCTCTTACTGTCCAGATCAATCAGTACAAGATTTTCAAGCAGCAAGTCGCTGCAACCATCCAAGCTCATGGCAATGTCAGCTACCTTCCAACCATGGATGCCTTCAGAACAGGGATTTATGTATTAGAAATTGGAGGCAATGACTTCAGCTTTGGGTACATGAATCTTCATCTTAATACCTCGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxACCCATTCCTCTAATGATTTTGATCAATATGGATGCTCCCTCTCGTACAACAATGCTGTTCGACTCTATAACAGAATGCTACGAGCGCAGCTCGCTGTTACCCGCAAGGAACTCCCGGATGCCACTATTATATATGTCAATACTTATGATATTGTCTATGACTTCTTTGTCAATCCCTCCAAATATGGTTTCAAGTTCACCACCAGGGCTTGCTGCGGAGTTGGGGGCAAATATAACTATAACTACGCAGTTCAATGTGGATCCACAGGAACCATAAAAAATACTTCTGTGACTGCAGAATCATGTCCTGATCCAGCTTCCTATGTCAACTGGGACGGAGTTCACTGGACAGATGAAGCCAACAAGATAATCACCCAGCAGATATTAAGTGGCAAATATTTCGAGCCTGCATTCTCCATTGCTTCACATTGTAATCTCCAACCTCTTTGA___AACTTCAAAACCCGTTCCTTTTTCCTGTAAAGATTCTTTAAAGGCCCATGAATAACACCGAGCGGCCAGGGTTCACTGTTTCTCTGCATGCAATACTGAGCAAAGTAATATTAGGTTTGAGTGTGGTTGGGTTCAAACCTGAGAGCGAGTTATTAATTGAAATTTATATTCTTTGGTACGTTGATTAATGAATAACCGTTGTGGCTCCTCGGCACGAGTGTCGACGCGCCTCATTAACGATGGGAGCAGAGAAGAGAGAGGAACGCATTGATCTCGACAATATCATTATGGGATTGTATTGCATGTGACACACCAGAGCTTTTTCTGAGTTAATATTAAATTAGCGTGAGCTATATCCTTGAAGCTCTGCTCGCTCCTTTTATTATGAT

Fln Alignment:

sp_v1.1_unigene24013___MIMTLLPAMCYSSCEYPAIFNFGDSTSDTGTIHYIFPYNMLAENPPYGRTFFGRPVNRYSDGRLSIDFFAQALGLPFLNPYLQSVXXXXXXXXXXXXXXXT-VRETNFIAPISLTVQINQYKIFKQQVAATIQAHGNVSYLPTMDAFRTGIYVLEIGGNDFSFxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFTHSSNDFDQYGCSLSYNNAVRLYNRMLRAQLAVTRKELPDATIIYVNTYDIVYDFFVNPSKYGFKFTTRACCGVGGKYNYNYAVQCGSTGTIKNTSVTAESCPDPASYVNWDGVHWTDEANKIITQQILSGKYFEPAFSIASHCNLQPL
B8LK87_______________LIGVLLPILCYGHCNFPAIFNFGDSSSDTGAIHFIFPNNELAENSPYGRTYFGKPVNRYCDGRLSIDFFATALGMPFLSPYLQSVDSSFGHGANFAAAGATAVSVDSFIAPIDLTVQINQFKVFKQQVLNTIKKHGAQSYLPSADAFDKGIYILEIGGNDFSYxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFGHSSNDFDSHGCSISYNDAVRYYNGLLKGQVGSLRGQLKGANVIYVNTYDILYDFIANPSRYGFKQTTRACCGVGGKYNYDYAVQCGISGTIAGHPVKAVSCAYPETYVNWDGVHWTDRANRILTKQILGGKYFEPAFSIASQCDIHSI

SNPs (tot: 80)

Position	A %	C %	G %	T %	Probability
83			75	25	0.996473
84			25	75	0.996473
85	25			75	0.996473
86		25		75	0.996473
87		25	75		0.996473
94			25	75	0.996473
121		30	70		1.0
124		10	90		0.90298
127		90	10		0.90298
129	70		10	20	0.999998
130	80		20		0.999996
131	70	10	20		0.999998
132		30		70	1.0
134			20	80	0.999996
135			10	90	0.90298
136		70	10		0.999998
138	90			10	0.90298
139		90	10		0.90298
142	10			90	0.90298
145	10			90	0.90298
290		9		90	1.0
291			9	90	1.0
293	90	9			1.0
294		90	9		1.0
296		90	9		1.0
298	90	9			1.0
301		90	9		1.0
303	90	9			1.0
304	90			9	1.0
308		9		90	1.0
310		9		90	1.0
311	90			9	1.0
313	9	90			1.0
406		11	88		1.0
412	86		13		1.0
413	83	16			1.0
415	83		13	3	1.0
417		83	3		1.0
420		13	86		1.0
421		83		16	1.0
446		78	21		1.0
453	78	21			1.0
455			78	21	1.0
456			81	18	1.0
459	77		19		1.0
461	77			22	1.0
462	77		22		1.0
467		22		77	1.0
469	76	16			1.0
470	16	83			1.0
492			93	6	0.999453
611		8		91	0.999766
616	91			8	0.999766
715	92			8	0.999718
721	91		8		0.999766
730	85	9	4		0.999726
732	90	9			0.999872
733	5	5		80	0.99967
772			88	11	0.999937
773		88	11		0.999937
775	88		11		0.999937
778	11			88	0.999937
783		11		88	0.999952
1136	87		12		0.992554
1164		62	37		1.0
1408	81		18		0.999994
1409		18		81	0.999994
1411			10	90	0.90298
1413	90		10		0.90298
1418	10		90		0.90298
1435	10		90		0.90298
1478	42		57		1.0
1492		20		80	0.995493
1506	72	27			1.0
1507	22		50	27	1.0
1508		22	77		1.0
1582		44		55	1.0
1632	55	45			1.0
1701		41		58	1.0
1728	40	59			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain