SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene23923

Length: 1416 nt

Origin of reads: Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene23923 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	PtrAUX2; auxin influx carrier component	-	-	45%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	88%
Blast2go	auxin influx carrier component	-	-	72%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Amino acid transporter, transmembrane	IPR013057	-	0.0	72%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKH8

Fln msg: Separated hits, possible frame ERROR between 102 and 128, your sequence is shorter than subject: 435 - 480

Fln protein: RRDLGCHSLWHGGSNYDAWCNAVSGQVGQVILSxxxxxxxxxxFGLGIFFHLLYSVVGIWTCYMLSCLYLEYRARKAKEGVHFKKHVIQYHEVMGYLVAPWLKGVCLFFNIVTMGTVAVVQIIACASNAYYLNSNYNKREWALIFGAISMLTVLLPSFHNFRIWSIIGVVTTTYTAWYMVIAGLLHGQIANVKHSAPVSMEQFFTGTTNILFAFGGHAITIEIMHAMWTPKAYKYVYLATVGYVMTITMPHCIILYWAFGDELLTHSNAFSVLPASTFRSIGLGFMIIHQAVAFGLYVMPLNFMWEKLLGVHQSRYLVRIIARLPVAMLLWLFALLFPFFGPLNSVIGSFIMSFSVYIIPCTAYLIVFRTKTSRQDASEKGGKWMPKWKGILTMNLGIVILVGVLGFGFGSWASLSNLVKQVNSFGILDKCYQC*

Protein Length: 436

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTGTGGCATGGTGGTTCCAATTATGATGCTTGGTGTAATGCTGTTTCTGGTCAGGTTGGCCAGGTGATCCTCTCCATxxxxxxxxxxxxxxxxxxxxxxxxxxxCTTCGGATTGGGAATTTTCTTCCATTTGTTGTATTCTGTTGTAGGTATCTGGACTTGCTACATGCTTTCATGCCTCTATTTGGAGTACCGAGCTCGGAAGGCAAAAGAAGGTGTCCACTTCAAGAAGCATGTTATCCAGTACCACGAGGTCATGGGCTACTTGGTTGCACCTTGGCTCAAGGGGGTTTGTCTCTTCTTCAACATTGTGACCATGGGAACTGTGGCAGTTGTACAAATTATTGCATGTGCAAGCAATGCATATTATCTGAATTCGAATTACAACAAAAGAGAGTGGGCACTCATATTTGGCGCCATATCGATGTTAACTGTCCTCCTTCCGTCCTTCCACAATTTCCGAATATGGTCTATCATTGGTGTGGTGACTACAACATATACAGCCTGGTACATGGTCATTGCGGGCCTTCTTCACGGCCAGATAGCAAATGTCAAACATTCTGCCCCTGTATCCATGGAGCAGTTTTTCACTGGGACTACCAACATACTATTTGCATTTGGAGGCCATGCAATTACCATAGAGATTATGCATGCTATGTGGACCCCAAAGGCATATAAATATGTGTACCTAGCAACGGTGGGGTATGTCATGACAATTACCATGCCACACTGTATAATACTCTACTGGGCATTTGGAGACGAGCTTTTAACGCATTCCAACGCCTTCTCAGTGTTGCCTGCTTCTACCTTCAGAAGCATAGGCTTGGGTTTCATGATTATTCACCAGGCAGTTGCCTTTGGGCTGTACGTGATGCCACTGAACTTCATGTGGGAAAAATTGCTGGGTGTACATCAATCACGTTATCTGGTCAGAATCATAGCGAGGCTCCCGGTGGCAATGTTGCTGTGGTTATTCGCATTGTTGTTTCCTTTCTTTGGGCCTCTTAACAGTGTAATAGGCTCTTTTATTATGAGCTTCTCGGTCTATATTATCCCATGTACCGCCTATCTCATTGTCTTCCGGACAAAAACGTCGAGACAGGACGCTTCGGAAAAGGGAGGAAAGTGGATGCCAAAATGGAAAGGGATTTTGACCATGAATTTGGGCATTGTCATCCTCGTCGGAGTGCTGGGATTCGGTTTTGGGAGTTGGGCGAGCCTTTCCAACCTGGTTAAACAAGTCAATTCATTTGGAATTCTTGACAAGTGTTATCAATGCTAGCACAAGTAATTTTGTCACCGTAGGTCTGTACAGGGGAAGACCCAAACCCAAATTTATGTAAATTGTGTGTGTGTGACGGGGATTCACGTTTGAGAATTAATAACAGTTGGGTA

Fln Alignment:

sp_v1.1_unigene23923___SLWHGGSNYDAWCNAVSGQVGQVILSMxxxxxxxxxFGLGIFFHLLYSVVGIWTCYMLSCLYLEYRARKAKEGVHFKKHVIQYHEVMGYLVAPWLKGVCLFFNIVTMGTVAVVQIIACXXXXXXXXXXXNKREWALIFGAISMLTVLLPSFHNFRIWSIIGVVTTTYTAWYMVIAGLLHGQIANVKHSAPVSMEQFFTGTTNILFAFGGHAITIEIMHAMWTPKAYKYVYLATVGYVMTITMPHCIILYWAFGDELLTHSNAFSVLPASTFRSIGLGFMIIHQAVAFGLYVMPLNFMWEKLLGVHQSRYLVRIIARXXXXXXXXXXXXXXXXXXXLNSVIGSFIMSFSVYIIPCTAYLIVFRTKTSRQDASEKGGKWMPKWKGILTMNXXXXXXXXXXXXXXGSWASLSNLVKQVNSFGILDKCYQC
B8LKH8_______________ALWHGGSRYDAWFNAVSGQVGQVILSMxxxxxxxxxFGLGIFFHLLYAGVGIWTCYMLSCLYLEYRSRKEREGADFKRHVIQYHEVMGYLVGSWLKKAALFFNIVTMGSVAVVQIIACASNAYYLNSKYNKREWAVIFGGISLLTCLLPSFHNFRVWSIMGVVTTTYTAWYMVIAGLLHGKIPDVKHSAPQDMEKFFTGTTNILFAFGGHAITIEIMHAMWQPKVYKYVYLWTVGYVLTITIPHCIVLYSNFGDELLNHSNALSVLPHSVFRSIALCFMICHQALAFGLYVMPLNFMWEKVLGVHGSTYLIRVVCRVPVVLLLWLLALVFPFFGPLNSMIGSLIMSFSVYIVPCVAYILVYKTKTSREEAAEKLPSWMPKWNGVVAINAAIVVLIAVLGFCLGSWASVSNLIKQVHNFGLFEKCYQC

SNPs (tot: 90)

Position	A %	C %	G %	T %	Probability
115			33	66	0.997452
135	66		33		0.997452
137	33		66		0.997452
180		75	25		0.996473
238		75		25	0.996473
259		25		75	0.996473
690		40	60		1.0
703			80	20	0.995493
704			20	80	0.995493
705	20			80	0.995493
711	20		80		0.995493
712		20		80	0.995493
714		80	20		0.995493
715	80	20			0.995493
718	20			80	0.995493
719	80		20		0.995493
720			20	80	0.995493
721			20	80	0.995493
722	20	20		60	0.99255
723	80		20		0.995493
724		20		80	0.995493
726		40		60	1.0
727	20		80		0.995493
728	25	75			0.996473
730		25		75	0.996473
731	25			75	0.996473
732	25			75	0.996473
735			75	25	0.996473
736		25	75		0.996473
737	25			75	0.996473
739		75	25		0.996473
742	75		25		0.996473
838		18	81		1.0
839	81		18		1.0
841		18		81	1.0
881		25		75	1.0
900	81		18		0.999994
914		81	18		0.999994
918	81		18		0.999994
954		60		20	0.999999
955			80	20	0.999996
958	80		20		0.999996
959			80	20	0.999996
962	20		80		0.999996
963	20		80		0.999996
964	80	20			0.999996
965		90		10	0.90298
966	20		10		0.999998
967	80		20		0.999996
969			20	70	0.999998
971	70	20	10		0.999998
974	80		20		0.999996
975	20		10	70	0.999998
976	20	60		20	0.999999
977		10	90		0.90298
980	75		25		1.0
981			12	87	0.992554
983	25		62	12	1.0
987	12	87			0.992554
991			28	14	1.0
992	71		28		1.0
994		28	14		1.0
996		28		71	1.0
999		28	42	28	1.0
1000	14	28	28	28	1.0
1004	20	80			0.995493
1006		80	20		0.995493
1009	20			80	0.995493
1011			40	60	1.0
1013	20			80	0.995493
1014		20		80	0.995493
1075	20	80			0.995493
1080		20		80	0.995493
1086			20	80	0.995493
1095		20		80	0.995493
1302		25		75	0.996473
1303		25	75		0.996473
1304	75	25			0.996473
1305	25		75		0.996473
1307		25	75		0.996473
1308	75			25	0.996473
1309		75	25		0.996473
1310	75		25		0.996473
1312	25	75			0.996473
1313		75	25		0.996473
1314	75			25	0.996473
1318	25			75	0.996473
1321		75		25	0.996473
1322	25		75		0.996473
1324		75	25		0.996473

SSRs (tot: 1)

Start position	End position	Sequence	Length
39	50	CA CA CA CA CA CA	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain