SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene22659

Length: 1581 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene22659

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Nuclease PA3, putative (EC:3.1.30.1)	-	-	57%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	86%
Blast2go	bifunctional nuclease	-	-	77%

Source	GOs	Term	Type	e value	Identity
Blast2go	single-stranded DNA specific endodeoxyribonuclease activity	GO:0000014	Molecular Function	0.0	77%
Blast2go	T/G mismatch-specific endonuclease activity	GO:0043765	Molecular Function	0.0	77%

Source	InterPros	Term	Type	e value	Identity
Blast2go	S1/P1 nuclease	IPR003154	-	0.0	77%
Blast2go	Phospholipase C/P1 nuclease domain	IPR008947	-	0.0	77%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACJ2

Fln msg: your sequence is longer than subject: 304 - 229

Fln protein: MGKFGCLMLFGMEILTLLVLLGVAPPLGVEAWGKEGHYSTCKIAEPLLSEEAAEAVRKLLPEYADGDLASLCSWPDQMRFRFHWASPLHYINTPDNQCGYSYERDCHDEHGVKGVCLEGALKNYTSQLEGYGSSGKYNLTQALLFLSHFMGDIHEPLHVGFTSDEGGNTIQLHWYKRKSNLHHVWDSYIIEKAMTEFYDNDLEVMIKAIQKNISGSWSDEVPTWEKCKGQEVVCPGEYADESIVIACKWAYKDAEEDSVLEDDYFLSRLPILERQLAKGGVRLAATLNRIFSSKKDHTRDSEL*

Protein Length: 305

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAGGATCTTGATCTTGTTCTTGTTCTTGTGTGTGATCAATACTTTTAGCATTCATTGGGTGAAGATTATATCAGCAGATTGGGTGATCAAGTTTTTTTTTGCCAGAGTTGCAGAAAACCAGCAGAGAGGTTTGAGTGATA_-_ATGGGCAAGTTTGGGTGTTTAATGTTGTTTGGAATGGAGATATTGACTCTCCTTGTCCTTCTTGGTGTGGCTCCTCCTCTTGGTGTGGAGGCTTGGGGAAAGGAAGGGCATTATTCCACTTGCAAGATAGCTGAGCCACTTCTAAGTGAAGAAGCAGCAGAGGCAGTGAGGAAATTGCTCCCCGAGTATGCAGATGGTGATCTCGCTTCTTTATGTTCGTGGCCTGATCAAATGCGATTCAGATTCCACTGGGCAAGTCCATTACACTACATTAACACACCAGATAACCAGTGTGGTTATAGTTATGAGAGGGACTGCCATGATGAACATGGAGTAAAGGGTGTGTGCCTTGAAGGTGCCTTAAAAAATTACACTTCTCAGCTTGAAGGTTATGGCAGCAGTGGAAAATATAATTTAACACAAGCTTTACTGTTTCTCTCTCATTTCATGGGAGATATCCATGAGCCTTTACATGTGGGATTCACCTCGGATGAAGGTGGAAATACAATACAGTTACACTGGTACAAGAGGAAGAGTAATTTGCACCATGTATGGGATAGTTACATCATCGAGAAAGCCATGACGGAATTTTATGACAATGATCTGGAGGTTATGATAAAAGCAATTCAGAAAAATATTTCCGGCAGCTGGTCTGATGAAGTACCTACATGGGAGAAATGCAAGGGACAAGAGGTCGTCTGCCCCGGAGAGTACGCAGATGAAAGTATCGTTATAGCTTGCAAGTGGGCTTACAAAGACGCTGAGGAAGACAGTGTTTTGGAAGATGACTATTTCCTCTCTCGCTTGCCCATTCTTGAGAGACAACTTGCAAAAGGAGGTGTGAGGTTGGCTGCAACACTCAACCGTATATTCAGTTCAAAAAAAGACCACACTCGGGATTCAGAACTCTAG___AAGCAACAGGACTGGGTTGTCTGCTACAACAGTCTTGTAAACTTAGATCAGGAACACTACCACTGTGAAAACTATCATCGTGACCATTGCACCGCAGCATCTTGGTTCCTCTGTGTATCAAGAAATAAACTGTACCCTAATTTTATAGGAGCATGAGTATCAATGATGTGTAATCATAACCTCTGTTACCATACCCATTTCAATGTTTTCCTAATTTTCCTTTAATGTAAATTGCCACGTATTTGAATTCCTTTAACCTTTGCCTGGACCTCCATCAGGTCGTTGAACAATGGGGTGGAATAACCGCAATCTGTATGAATATGATGTATCATTGAACAATGGGGTTGAATAACCGCAATCTGTATGAATATGATGTATCATTGATTGATTTATTACTAACAATGCCTCCAAAGTGCAAAGGGAAAAAAATGTTAATTTGGGATTATTGAATTGTAAAAGTTATACTTTGGTTTCAAATAATAATTATTGTATTAAATTTAGTTGCC

Fln Alignment:

sp_v1.1_unigene22659___MRFRFHWASPLHYINTPDNQCGYSYERDCHDEHGVKGVCLEGALKNYTSQLEGYGS---SGKYNLTQALLFLSHFMGDIHEPLHVGFTSDEGGNTIQLHWYKRKSNLHHVWDSYIIEKAMTEFYDNDLEVMIKAIQKNISGSWSDEVPTWEKCKGQEVVCPGEYADESIVIACKWAYKDAEEDSVLEDDYFLSRLPILERQLAKGGVRLAATLNRIFSSKKDHTRDSEL
D5ACJ2_______________MRFRFHWASPLHYINTPDNQCGYDYERDCHDEHGVKGVCLEGAIKNYTSQLETYGSRATGGKYNLTQALLFLSHFMGDVHEPLHVGFTSDEGGNTIQLHWYKRKCNLHHVWDSFIIEKAMKEYYDNDLEVMIEAIQKNISDSWSDEVTTWEKCNGRELACPGSYADESIILACKWAYKDAKENSVLEDDYFLSRLPILEKQLARGGVRLAATLNRIFSSKEGYTRESEL

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
1206	10		90		0.90298
1224	85			14	0.993533
1225	71		14	14	0.984337
1226	71		14	14	0.984337
1398	84	15			1.0
1407		85	14		0.993533
1474		40		60	1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
35	52	TCTTGT TCTTGT TCTTGT	18

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain