SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene22270

Length: 1394 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene22270

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Elongation factor 2 n=16 Tax=Magnoliophyta RepID=EF2_BETVU	-	-	85%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	34%
Blast2go	elongation factor 2	-	-	95%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	95%
Blast2go	copper ion binding	GO:0005507	Molecular Function	95%
Blast2go	plasma membrane	GO:0005886	Cellular Component	95%
Blast2go	chloroplast	GO:0009507	Cellular Component	95%
Blast2go	cytosol	GO:0005829	Cellular Component	95%

Source	InterPros	Term	Type	Identity
Blast2go	Protein synthesis factor, GTP-binding	IPR000795	-	95%
Blast2go	Small GTP-binding protein domain	IPR005225	-	95%
Blast2go	Translation elongation/initiation factor/Ribosomal, beta-barrel	IPR009000	-	95%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LKZ4

Fln msg: Unexpected stop codon in the beginning of your sequence, Distance to subject end: 307 aas, your sequence is shorter than subject: 464 - 785

Fln protein: RRDLGCHSS*PWLSLIIVTKSNQLQRVQTNCS*TQVLIGIQE*GKMVKFTAEGLRTIMDRKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYEMEDAAVKNFKGDREGHEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSRVIENANVIMATYEDPLLGDCQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKMMERLWGENFFDPATKKWTTKNTGSATCKRGFVQFIYEPIKQIINICMNDQKDKLWPMLQKLGVVMKSEEKELVAKALMKRVMQTWLPASSALLEMMIFHLPSPAKAQRYRVENLYEGPLDDIYATAIRNCDPEGPLMLYVSKMIPASDKGRFFAFGRVFAGRVATGMKVRIMGPNYVPG

Protein Length: 465

Fln nts: CGTCGTGACCTTGGCTGTCACTCGTCGTGACCTTGGCTGTCACTCATTATCGTTACGAAGAGCAACCAGCTGCAAAGAGTACAAACGAATTGCAGCTAGACACAGGTGCTCATTGGTATTCAAGAGTAAGGCAAAATGGTGAAGTTCACAGCTGAAGGGCTTCGTACGATCATGGACAGAAAGCATAACATACGAAACATGTCTGTTATTGCCCATGTTGACCACGGAAAATCCACTCTCACTGATTCTTTGGTAGCAGCAGCTGGTATCATTGCGCAAGAAGTTGCCGGAGATGTCAGAATGACTGATACTCGGGCAGATGAAGCTGAACGTGGCATCACCATTAAGTCAACTGGAATTTCATTGTACTATGAGATGGAGGATGCAGCTGTGAAAAATTTCAAGGGGGATAGAGAAGGACATGAATATTTGATAAATCTGATTGATTCTCCAGGGCACGTTGACTTTTCTTCAGAAGTTACAGCAGCCCTGCGAATCACAGATGGTGCACTGGTTGTGGTAGATTGTATTGAAGGGGTTTGTGTCCAGACTGAGACTGTTCTTCGTCAAGCTCTTGGAGAAAGGATTAGGCCTGTTCTCACCGTGAACAAGATGGACAGGTGCTTTCTTGAGTTGCAGGTTGATGGAGAGGAAGCTTACCAAACCTTCTCACGTGTCATTGAAAATGCAAATGTGATTATGGCTACTTATGAAGATCCTCTTCTTGGTGACTGCCAAGTTTATCCTGAAAAAGGCACAGTTGCATTCTCAGCAGGTCTGCATGGGTGGGCATTTACCCTCACAAATTTTGCAAAAATGTATGCTTCAAAGTTTGGAGTGGATGAAGCAAAGATGATGGAGAGGCTCTGGGGTGAAAACTTTTTTGATCCAGCTACCAAGAAATGGACTACCAAGAATACAGGTTCTGCAACTTGCAAAAGAGGATTTGTTCAATTCATATATGAACCTATTAAGCAAATTATCAATATTTGTATGAATGATCAAAAGGATAAGTTGTGGCCCATGTTGCAGAAATTGGGAGTTGTAATGAAAAGTGAAGAGAAAGAGCTGGTTGCAAAAGCACTCATGAAGCGTGTCATGCAAACCTGGCTACCTGCAAGTAGTGCTTTACTTGAAATGATGATTTTCCATCTTCCTTCTCCAGCGAAGGCACAGAGATATCGTGTGGAAAACTTGTATGAAGGACCTCTTGATGATATATATGCAACTGCAATAAGAAATTGTGACCCTGAGGGTCCACTGATGTTGTATGTTTCAAAAATGATTCCAGCTTCGGACAAAGGACGTTTCTTTGCCTTTGGTCGTGTTTTTGCTGGGAGAGTTGCAACAGGTATGAAGGTAAGGATCATGGGTCCCAACTATGTGCCAGGTCA

Fln Alignment:

sp_v1.1_unigene22270___RNMSVIAHVDHGKSTLTDSLVAAAGIIAQ--EVAGDVRMTDTRADEAERGITIKSTGISLYYEMEDAAVKNFKGDREGHEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSRVIEN
B8LKZ4_______________RNIGIMAHIDAGKTTTTERILFYTGKNYKIGEVHDGTATMDWMEQEQERGITITSAATTCAW----------------NNHRINIIDTPGHVDFTLEVERALRVLDGSICLFDSVAGVEPQSETVWRQADRYGVPRICFVNKMDRL-------GANFFRTRDMIITN

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
25	25			75	0.996473
26	25	75			0.996473
27			75	25	0.996473
29	25		75		0.996473
31		75	25		0.996473
35			75	25	0.996473
36			75	25	0.996473
39	50		50		1.0
41		75		25	0.996473
42	75			25	0.996473
89	75	25			0.996473
546		80		20	0.995493
657		10		90	0.90298
1004	83	16			0.994512
1218			12	87	0.992554
1219	87			12	0.992554
1220			12	87	0.992554
1222		12		87	0.992554
1224			12	87	0.992554
1227	87	12			0.992554
1286		25		75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain