SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene22160

Length: 1406 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene22160

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Probable rhamnose biosynthetic enzyme 1 n=2 Tax=Arabidopsis RepID=RHM1_ARATH	-	-	89%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	27%
Blast2go	protein	-	-	96%

Source	ECs	Term	Type	e value	Identity
Blast2go	UDP-glucose 4,6-dehydratase.	EC:4.2.1.76	-	0.0	96%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Amino sugar and nucleotide sugar metabolism	00520		0.0	96%

Source	GOs	Term	Type	Identity
Blast2go	UDP-rhamnose biosynthetic process	GO:0010253	Biological Process	96%
Blast2go	seed coat development	GO:0010214	Biological Process	96%
Blast2go	flavonol biosynthetic process	GO:0051555	Biological Process	96%
Blast2go	mucilage biosynthetic process	GO:0010192	Biological Process	96%
Blast2go	auxin efflux	GO:0010315	Biological Process	96%
Blast2go	UDP-glucose 4,6-dehydratase activity	GO:0050377	Molecular Function	96%
Blast2go	UDP-L-rhamnose synthase activity	GO:0010280	Molecular Function	96%
Blast2go	soluble fraction	GO:0005625	Cellular Component	96%

Source	InterPros	Term	Type	e value	Identity
Blast2go	NAD-dependent epimerase/dehydratase	IPR001509	-	0.0	96%
Blast2go	NAD(P)-binding domain	IPR016040	-	0.0	96%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NUL8

Fln msg: Distance to subject end: 55 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 269 - 417

Fln protein: MAPESYTPKNILITGAAGFIASHVANRLVRKYPQYKIVVLDKLDYCSNLKNLLPSKSSPNFKFVRGDIASADLVNYLLITEGIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIRRFIHVSTDEVYGETEQDALVGNHEASQLLPTNPYSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQFPEKLIPKFILLAMQGKPLPIHGDGSNVRSYLYCEDVAEAFEVVLHSGEVGNVYNIGTKKERRVIDVAKDICK

Protein Length: 270

Fln nts: CGTCGTGACCTTGGCTGTCACTCAACTTTCAGAATTAAAAGCGCCGAATGGGAATATAACACACGCTTTCTCTGCACAACACGGCCCGACCCGACCCGCCTCTCTCTGCATAACGAAGACCATAATCGTGAAAGCTGCAAAAAGTGACGTGGGTTGAAGAAACGATGAAGGGGATCGTAACTTGTTTTTTAACTACAAATCAGGCTTTTCAAAGGCTGTGATTGCGGGACAGGTAAACACCCGTTTCAAACCTGACTACTCCTGCCGCTTCTCATCATCTTCCTCGGCGACCCTTCAAATAGTTAAAATGTGCCCTTTAACACAACTGCCAGGCGTCCGGGCTATATAGGTAATATCTATCCATTCATTCGCTCAAGGCATCCGAAGTTCTGCTTGAGAGACGCACAAAAATCGAATTGTTGACCTCTGAAGGGTCCACAAGAAAACACCCGTTACTTCATTCGAGTATTTCCTGCATTTTCCGCAGCAAGATCTGAGCCTAAACATCATTTGGGTATTTTTTACCCTGGATCTGCGCACTTTGGTTTCTTTCAGGGGAGATCTGGTTGTGTCCTTGACTTAATAAACTGAATAAAA_-_ATGGCTCCTGAGAGCTATACCCCCAAGAACATTCTCATCACGGGTGCTGCTGGATTTATTGCTTCTCATGTAGCGAATAGGCTGGTCAGAAAATACCCCCAGTATAAGATTGTTGTGCTTGATAAACTGGATTATTGCTCCAATTTGAAGAATCTGTTGCCTTCAAAATCATCCCCCAATTTCAAATTTGTAAGGGGAGATATTGCGAGTGCAGATCTGGTGAATTATCTTCTAATCACAGAAGGAATAGACACGATCATGCATTTTGCAGCACAGACCCACGTTGACAATTCGTTTGGGAACAGCTTCGAGTTCACGAAGAATAATATCTACGGAACTCATGTCCTATTGGAGGCTTGTAAAGTTACGGGGCAGATTAGGCGGTTCATTCATGTCAGTACTGATGAAGTTTATGGAGAGACTGAGCAGGATGCTCTTGTGGGTAACCACGAGGCCTCCCAGCTTCTTCCTACGAATCCTTACTCTGCTACCAAGGCTGGAGCAGAAATGCTTGTCATGGCCTACGGAAGATCTTATGGCTTGCCGGTGATTACTACCAGAGGTAACAATGTCTATGGGCCCAATCAGTTCCCTGAGAAGCTCATTCCAAAATTCATTCTTTTGGCCATGCAGGGTAAACCCCTTCCCATTCATGGAGATGGATCGAATGTTCGAAGCTATCTCTACTGTGAGGATGTGGCAGAAGCATTTGAGGTTGTTTTGCACAGTGGCGAAGTAGGAAATGTTTATAACATCGGTACAAAGAAAGAGAGGAGAGTTATTGATGTGGCTAAGGATATATGCAAACT

Fln Alignment:

sp_v1.1_unigene22160___PKNILITGAAGFIASHVANRLVRKYPQYKIVVLDKLDYCXXXXXXXXXXXXXXFKFVRGDIASADLVNYLLITEGIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIRRFIHVSTDEVYGETEQDALVGNH--EASQLLPTNPYSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQFPE--KLIPKFILLAMQGKPLPIHGDGSNVRSYLYCEDVAEAFEVVLHSGEVGNVYNIGTKKERRVIDVAK
A9NUL8_______________PLRIVVTGGAGFVGSHLVDRLLERGDH--VIVIDNF------FTGRKENVMHHFKNPRFELIRHDVVEPILLE--VDQIYHLACPASPVHYKFNPVKTIKTNVMGTLNMLGLAKRIGA--RFLLTSTSEVYGDPLQHPQTETYWGNVNPIGVRSCYDEGKRTAETLTMDYHRGANVEVRIARIFNTYGPRMCIDDGRVVSNFVAQALRKEPLTVYGDGKQTRSFQYVSDLVEGLIRLMESNHVG-PFNLGNPGEFTMLELAE

SNPs (tot: 59)

Position	A %	C %	G %	T %	Probability
47		75		25	0.996473
51	75	25			0.996473
53			75	25	0.996473
57	75	25			0.996473
59	75		25		0.996473
61	75	25			0.996473
64	75			25	0.996473
92	42		57		1.0
317		88		11	0.923564
319			11	88	0.923564
321			11	88	0.923564
323	88	11			0.923564
325	88			11	0.923564
326		88		11	0.923564
327	88		11		0.923564
328	88		11		0.923564
332		88		11	0.923564
334	75	25			1.0
336			66	33	1.0
338	8			25	1.0
340	25			75	1.0
343			69	23	1.0
348	76		23		1.0
351		23		76	1.0
354		23	76		1.0
356	76	23			1.0
427	90		10		0.90298
531		90		10	0.90298
535	90	10			0.90298
537		90	10		0.90298
539	10		90		0.90298
541			90	10	0.90298
703			80	20	0.995493
712		11		88	0.923564
715		11		88	0.923564
723	88	11			0.923564
725	88			11	0.923564
726	88	11			0.923564
730		11	88		0.923564
731	11		88		0.923564
887		11	88		0.923564
888	88		11		0.923564
891	88		11		0.923564
893			11	88	0.923564
894	11	88			0.923564
897			11	88	0.923564
898	11			88	0.923564
899		11	88		0.923564
900	11		88		0.923564
902	88		11		0.923564
903	88	11			0.923564
913		10	89		0.999919
916		89	10		0.999919
917	89		10		0.999919
919			89	10	0.999919
920	89		10		0.999919
921	89			10	0.999919
922		10	89		0.999919
924	89	10			0.999919

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain