SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene21755

Length: 2177 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene21755
CGCGTGACCTTGGCTGTCACTCATTGGCGTTGTTTGCATTGGTGGTAGAAGTGGAAGAGGAAAAAATATGTCTGATCTAGAGCAGCCAATGTTACGTCGGACCTTGGCTGTCACTCAGGTTTGGGTGGATTACTTAGTGAACTTCAGATGGATATTAGTGATTTTTGTTGTTCTGCCCCTGTCATTCACATTCTATTTTATGACCTATTTGGGGGATGTTTGGTCTGAGAGCAAATCCTTCAAGCGTAGGCAGAAGGAGCACGACGAGAATGTGGAGAAGGTCATTAAACGTCTCAAGGAGAGAAACCCATCTAAAGATGGCCTCGTTTGCACTGCTAGAAAGCCTTATATTGCTGTGGGTATGCGCAATGTGGACTACAAGAGGGCCAGACACTTCGAGGTGGATCTGTCTGAATTCAGAAACATACTTGAAATTGACAAGGAGAAAATGATTGCGAGAGTAGAACCCCTGGTCAATATGGGTCAGATTACTAGGGTGACCGTGCCCATGAATCTGGCCCTGGCAGTGGTGGCCGAGCTGGATGATCTTACTGTTGGTGGGTTGATCAATGGGTATGGGATAGAAGGGAGTTCGCATATCTATGGGCTCTTTTCTGATACTGTTGTGGCATACGAGGTTGTGTTAGCCGATGGCCGATTAGTTAGGGCCACCAAGGATAACGAGTTTTCTGATCTTTTCTATGCAATCCCATGGTCCCAGGGTACACTTGGGCTTCTGGTAGCAGCAGAGATCAAGCTCATACCAATAAAGGAATACATGAGAGTAACCTATACGCCTGCTAGGGGTAACCTCAGGGAGTTAGCCCAGGCCTACATTGATTCCTTTGCACCCAGGGATGGAGATGAGTCCAAGGTCCCAGACTTTGTGGAGGGCATGATATATAACCCCACAGAAGCCGTGCACATGACAGGAAGATATGCCTCCAAGGAAGAAGCGAAGCAAAAGGGTAATGTTATCAACAGTGTTGGATGGTGGTTCAAGCCCTGGTTCTACCAGCACGCTCAGACTGCTCTTAAGAGAGGGGAGTTCGTTGAGTACATTCCCACTAGGGAGTACTACCACCGGCATACTCGGTGTCTCTATTGGGAGGGAAAGCTCATCTTGCCTTTCGCTGACCAATGGTGGTTCCGATGGACGTTGGGATGGCTCATGCCTCCAAAGGTCTCGTTTCTTAAGGCCACTCAGGGTGACGCTATAAGAAATTATTATCATGAAATGCATGTTATTCAGGATATGCTTGTTCCTCTGTACAAGGTCGCAGATGCGCTCGAGTTTCAGCACCGTGAGATGGAGGTATATCCTATTTGGCTGTGCCCTCATCGCCTCTTCCAACTTCCTGTGAAGACAACTGTCTACCCAGAGCCTGGTTTTGAGGAGGCGCAGAGGCAAGGTGACACAGAATATGCCCAGATGTACACAGATGTAGGCATCTATTATACTCCAGCTTGTGTCTTCCGTGGGGAGGCATTTGATGGAGCAGAAGCAGTGCGGAGAATGGAGAAATGGTTGATTGAAAATCATGGGTTTCAGCCACAATATGCTGTTTCAGAATTGAGTGAGAGAGACTTCTGGCGCATGTTTGATGGTTCACTCTACAACAGTTGCAGAGAAAAATACAGAGCCGTGGGAACTTTCATGAGTGTCTACTACAAAAGCAAGAAGGGAAGAAAGACTGAAAAGGAGGTTCAAGAGGAAGAGCAAAAGCAACTGGACAATGTATATGTCGAGTTGGACCAACCTGTGATGGAGTAACTACGAATGCTTGATTGAGGAGACCTTACAAATTTTATCGACAAATTCAGTTCATTTTGATTTCAAATTCTGATCTTTTTTTTTCCTGTGCGTGTGGGTAAATTACCATTGGGAACAGTTAGTAGTACACAGAGCATTAGTCTCCAGGCATATTTTGAAGGTTATTTTGGGCTAGTCTGAAGCCTACTCACACAGTAGATCGCTTTAATTTTGAGAGACATTTTATCATATTTTTCCTTTGGCATGTTTGTTTGTGATTTACCAATGTATGTTTTTAATAACCTTTGGATCTTAGCCGTTCTCACAGTTGAATTTTGGCTATAGCTTTCAGTTCTTGATATCGAAAGTTAAATTTTTTTAAATACAAAGCACTGATCTGATGGAGATACATTTTCTCTGCTAC

Ace file of the UniGene sp_v1.1_unigene21755

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cell elongation protein diminuto, putative; K09828 delta24-sterol reductase [EC:1.3.1.72]	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	cell elongation protein	-	-	89%

Source	ECs	Term	Type	e value	Identity
AutoFact	Delta(24)-sterol reductase.	EC:1.3.1.72	-	0.0	77%

Source	KEGGs	Term	Identity
AutoFact	Steroid biosynthesis	00100	77%
AutoFact	Biosynthesis of terpenoids and steroids	01062	77%
AutoFact	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	77%
AutoFact	Biosynthesis of plant hormones	01070	77%
AutoFact	Metabolic pathways	01100	77%
AutoFact	Biosynthesis of secondary metabolites	01110	77%

Source	GOs	Term	Type	Identity
Blast2go	brassinosteroid biosynthetic process	GO:0016132	Biological Process	89%
Blast2go	unidimensional cell growth	GO:0009826	Biological Process	89%
Blast2go	response to light stimulus	GO:0009416	Biological Process	89%
Blast2go	catalytic activity	GO:0003824	Molecular Function	89%
Blast2go	calmodulin binding	GO:0005516	Molecular Function	89%
Blast2go	plasma membrane	GO:0005886	Cellular Component	89%
Blast2go	integral to membrane	GO:0016021	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Blast2go	FAD linked oxidase, N-terminal	IPR006094	-	89%
Blast2go	FAD-binding, type 2	IPR016166	-	89%
Blast2go	FAD-linked oxidase, FAD-binding, subdomain 2	IPR016168	-	89%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AAF3

Fln msg: your sequence is longer than subject: 569 - 390

Fln protein: MSDLEQPMLRRTLAVTQVWVDYLVNFRWILVIFVVLPLSFTFYFMTYLGDVWSESKSFKRRQKEHDENVEKVIKRLKERNPSKDGLVCTARKPYIAVGMRNVDYKRARHFEVDLSEFRNILEIDKEKMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEVVLADGRLVRATKDNEFSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMRVTYTPARGNLRELAQAYIDSFAPRDGDESKVPDFVEGMIYNPTEAVHMTGRYASKEEAKQKGNVINSVGWWFKPWFYQHAQTALKRGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWWFRWTLGWLMPPKVSFLKATQGDAIRNYYHEMHVIQDMLVPLYKVADALEFQHREMEVYPIWLCPHRLFQLPVKTTVYPEPGFEEAQRQGDTEYAQMYTDVGIYYTPACVFRGEAFDGAEAVRRMEKWLIENHGFQPQYAVSELSERDFWRMFDGSLYNSCREKYRAVGTFMSVYYKSKKGRKTEKEVQEEEQKQLDNVYVELDQPVME*

Protein Length: 570

Fln nts: CGCGTGACCTTGGCTGTCACTCATTGGCGTTGTTTGCATTGGTGGTAGAAGTGGAAGAGGAAAAAAT_-_ATGTCTGATCTAGAGCAGCCAATGTTACGTCGGACCTTGGCTGTCACTCAGGTTTGGGTGGATTACTTAGTGAACTTCAGATGGATATTAGTGATTTTTGTTGTTCTGCCCCTGTCATTCACATTCTATTTTATGACCTATTTGGGGGATGTTTGGTCTGAGAGCAAATCCTTCAAGCGTAGGCAGAAGGAGCACGACGAGAATGTGGAGAAGGTCATTAAACGTCTCAAGGAGAGAAACCCATCTAAAGATGGCCTCGTTTGCACTGCTAGAAAGCCTTATATTGCTGTGGGTATGCGCAATGTGGACTACAAGAGGGCCAGACACTTCGAGGTGGATCTGTCTGAATTCAGAAACATACTTGAAATTGACAAGGAGAAAATGATTGCGAGAGTAGAACCCCTGGTCAATATGGGTCAGATTACTAGGGTGACCGTGCCCATGAATCTGGCCCTGGCAGTGGTGGCCGAGCTGGATGATCTTACTGTTGGTGGGTTGATCAATGGGTATGGGATAGAAGGGAGTTCGCATATCTATGGGCTCTTTTCTGATACTGTTGTGGCATACGAGGTTGTGTTAGCCGATGGCCGATTAGTTAGGGCCACCAAGGATAACGAGTTTTCTGATCTTTTCTATGCAATCCCATGGTCCCAGGGTACACTTGGGCTTCTGGTAGCAGCAGAGATCAAGCTCATACCAATAAAGGAATACATGAGAGTAACCTATACGCCTGCTAGGGGTAACCTCAGGGAGTTAGCCCAGGCCTACATTGATTCCTTTGCACCCAGGGATGGAGATGAGTCCAAGGTCCCAGACTTTGTGGAGGGCATGATATATAACCCCACAGAAGCCGTGCACATGACAGGAAGATATGCCTCCAAGGAAGAAGCGAAGCAAAAGGGTAATGTTATCAACAGTGTTGGATGGTGGTTCAAGCCCTGGTTCTACCAGCACGCTCAGACTGCTCTTAAGAGAGGGGAGTTCGTTGAGTACATTCCCACTAGGGAGTACTACCACCGGCATACTCGGTGTCTCTATTGGGAGGGAAAGCTCATCTTGCCTTTCGCTGACCAATGGTGGTTCCGATGGACGTTGGGATGGCTCATGCCTCCAAAGGTCTCGTTTCTTAAGGCCACTCAGGGTGACGCTATAAGAAATTATTATCATGAAATGCATGTTATTCAGGATATGCTTGTTCCTCTGTACAAGGTCGCAGATGCGCTCGAGTTTCAGCACCGTGAGATGGAGGTATATCCTATTTGGCTGTGCCCTCATCGCCTCTTCCAACTTCCTGTGAAGACAACTGTCTACCCAGAGCCTGGTTTTGAGGAGGCGCAGAGGCAAGGTGACACAGAATATGCCCAGATGTACACAGATGTAGGCATCTATTATACTCCAGCTTGTGTCTTCCGTGGGGAGGCATTTGATGGAGCAGAAGCAGTGCGGAGAATGGAGAAATGGTTGATTGAAAATCATGGGTTTCAGCCACAATATGCTGTTTCAGAATTGAGTGAGAGAGACTTCTGGCGCATGTTTGATGGTTCACTCTACAACAGTTGCAGAGAAAAATACAGAGCCGTGGGAACTTTCATGAGTGTCTACTACAAAAGCAAGAAGGGAAGAAAGACTGAAAAGGAGGTTCAAGAGGAAGAGCAAAAGCAACTGGACAATGTATATGTCGAGTTGGACCAACCTGTGATGGAGTAA___CTACGAATGCTTGATTGAGGAGACCTTACAAATTTTATCGACAAATTCAGTTCATTTTGATTTCAAATTCTGATCTTTTTTTTTCCTGTGCGTGTGGGTAAATTACCATTGGGAACAGTTAGTAGTACACAGAGCATTAGTCTCCAGGCATATTTTGAAGGTTATTTTGGGCTAGTCTGAAGCCTACTCACACAGTAGATCGCTTTAATTTTGAGAGACATTTTATCATATTTTTCCTTTGGCATGTTTGTTTGTGATTTACCAATGTATGTTTTTAATAACCTTTGGATCTTAGCCGTTCTCACAGTTGAATTTTGGCTATAGCTTTCAGTTCTTGATATCGAAAGTTAAATTTTTTTAAATACAAAGCACTGATCTGATGGAGATACATTTTCTCTGCTAC

Fln Alignment:

sp_v1.1_unigene21755___GLFSDTVVAYEVVLADGRLVRATKDNEFSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMRVTYTPARGNLRELAQAYIDSFAPRDGDESKVPDFVEGMIYNPTEAVHMTGRYASKEEAKQKGNVINSVGWWFKPWFYQHAQTALKRGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWWFRWTLGWLMPPKVSFLKATQGDAIRNYYHEMHVIQDMLVPLYKVADALEFQHREMEVYPIWLCPHRLFQLPVKTTVYPEPGFEEAQRQGDTEYAQMYTDVGIYYTPACVFRGEAFDGAEAVRRMEKWLIENHGFQPQYAVSELSERDFWRMFDGSLYNSCREKYRAVGTFMSVYYKSKKGRXXXXXXXXXXXXXLDNVYVELDQPVME
D5AAF3_______________GSFSDTVVAFEVVLADGRLVRATKDNEFSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMRLTYMPARGNLRELAQAYIDSFAPRDGDQSKVPDFVESMIYTPTEAVHMTGRYASKEEAKKKGNVINSVGWWFKPWFYQHAQTALKRGEFVEYIPTRQYYHRHTRCLYWEGKLILPFADQWWFRWTMGWLMPPKVSFLKATQGEAIRNYYHEMHVIQDMLVPLYKVADALEFQDREMEIYPIWLCPHRLFKLPVKTTVYPEPGFEEAHRQGDTEFAQMYTDVGIYYTPAPVFRGEAFDGAEAVRRMEKWLIENHGFQPQYAVSELNERDFWRMFDGSLYNSCREKYRAVGTFMSVYYKSKKGRKTEKEVREEEQKQLDNAYAELDQPVME

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
974	33			66	1.0
1008		80		20	0.995493
1061	80		20		0.995493
1315	18		81		0.999994
1442	25	75			0.996473
1444		25	75		0.996473
2135		16		83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain