SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene21068

Length: 810 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene21068 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	oxidoreductase, 2OG-Fe(II) oxygenase family protein (EC:1.14.11.9)	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	53%
Blast2go	fe ascorbate oxidase	-	-	75%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Oxoglutarate/iron-dependent oxygenase	IPR005123	-	0.0	75%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: A9P1C4

Fln msg: Overlapping hits, possible frame ERROR between 234 and 150, Distance to subject end: 61 aas, your sequence is shorter than subject: 270 - 363

Fln protein: KYILPPNERPQMSEVCCYSENIPVVDLKDLDGPKRSKVVEEIRRACEEDxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxEDRACLYSEDPNQNVRLSTSFNIAKEKVLNWRDYLLHPCHPLEEVIGSWPEKPAAYREIASKYAIEVRALVLRLLASISEALGLSSDYLNSILGKHNQGMTVNYYPPCPNPDLTLGLTGHSDASAITVLMQGDVNGLQVLKNGKWVSVEPIANAFVINLGDHLQVVSNGKFRSVEHRAVTNASTARISIST

Protein Length: 271

Fln nts: AAATATATCTTGCCTCCCAATGAACGCCCACAGATGTCCGAGGTTTGCTGTTACTCTGAAAACATTCCGGTGGTGGACCTCAAAGACCTGGATGGACCAAAAAGAAGCAAGGTTGTCGAAGAAATCCGACGTGCCTGTGAGGAGGATxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGAAGACAGAGCATGCCTCTACTCAGAGGACCCAAATCAGAATGTTCGGCTTTCTACGAGCTTCAATATTGCCAAAGAAAAAGTCCTTAACTGGAGAGATTATCTGCTGCATCCATGTCATCCTCTGGAAGAAGTGATAGGCTCTTGGCCTGAAAAGCCTGCAGCTTACAGAGAAATTGCAAGTAAATATGCTATAGAAGTGAGAGCACTGGTTCTAAGACTGCTGGCTTCCATTTCTGAAGCTCTAGGTCTCAGCTCTGATTACTTGAATAGTATTCTTGGAAAACACAACCAAGGTATGACTGTCAATTACTATCCTCCATGCCCAAACCCAGATCTTACCCTTGGTTTAACAGGCCATTCAGACGCAAGTGCCATCACTGTTCTAATGCAAGGCGATGTTAATGGCTTGCAAGTACTCAAAAATGGAAAATGGGTTTCTGTAGAACCCATCGCTAACGCCTTTGTCATTAATTTGGGAGATCATCTTCAGGTAGTGAGCAATGGGAAATTCAGGAGTGTTGAGCATCGAGCAGTAACGAACGCATCCACTGCACGTATATCAATTTCTACGT

Fln Alignment:

sp_v1.1_unigene21068___YILPPNERPQMSEVCCYSENIPVVDLKDLDGPKRSKVVEEIRRACEEDGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYSEDPNQNVRLSTSFNIAKEKVLNWRDYLLHPCHPLEEVIGSWPEKPAAYREIASKYAIEVRALVLRLLASISEALGLSSDYLNSILGKHNQGMTVNYYPPCPNPDLTLGLTGHSDASAITVLMQGDVNGLQVLKNGKWVSVEPIANAFVINLGDHLQVVSNGKFRSVEHRAVTNASTARISIST
A9P1C4_______________YILPPSERPRLSEVS-YSESIPVVDLQDLDGPNRTRVVQEICLACEEDGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYSEDTNQRVRLSTSFNISKEKVLNWRDYLLHPCHPLEEFIGSWPEKPAAYREIAGNYAAEMRALILRLMAAVSEALGLDSDYLNRIFGKHSQLMDIMYYPPCPNPDITLGTPRHSDARGITVLMQGNVSGLQVLRNGKWVAVEPIPNAFVVNMGDQLQVVSNGRFRSVEHRAVTNTSTARISIPT

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
505		25	75		0.996473
519		75		25	0.996473
521			25	75	0.996473
522	75			25	0.996473
535		50		50	1.0
537		75		25	0.996473
540	75	25			0.996473
569			25	75	0.996473
576		25	75		0.996473
586		25		75	0.996473
615	75		25		0.996473
633		25		75	0.996473
649	75		25		0.996473
738		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain