SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene20901

Length: 2215 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene20901
CGTCGTGACCTTGGCTGTCACTCAACATTGGTTTTGTTTGGCATAGCCAATATAAAGGAGTCGAAGAAGGTTTGGTGTTAAGGATTGTAAGGGATACCTACTCGCCTATTCATTGTTCCGGGTAGCTGCAACTGTGGTTCGAACCTAGAACGTTAGCTAGAGGCAAGCCGTCTGTATTAGGCCGGGAATTATTGAAAAGTTCGGCCTTGAACGACTCTCTGAAGCTGCGGATCCATTTCTTGGTGAGGATCAGAGTTAATTGTCCTCGTCTAATCTTTTTGCGAATCCAAGCTGCTGTAGATTCATCTTAGGTTTTGTAATGGCCAACATCGACATTGAGGGTATTTTGAAGGAAATTCCCAATGATGGGAGGGTACCAAAGACCAAGATTGTGTGTACACTGGGCCCTGCCTCTAGGAGTGTTGAGATGATCGAGAAGCTGCTGAGGGCTGGGATGAATGTTGCCCGATTCAACTTCTCTCACGGTTCACATGAGTATCACCAAGAGACGCTTGATAATCTCAGGACAGCGATGTATAATACGCAGATAATGTGTGCTGTTATGCTTGATACCAAGGGGCCTGAGATTCGAACTGGATTTTTGAAGGATGCAAAAGCAATCCAACTTAGGGAAGGTCAGGAAATCACAATAACTACAGATTACACTATTAAGGGTGATACTAACATGATCTCCATGAGCTATAAAAAACTGGCAGAAGATTTAAGTCCTGGTAATACAATCTTGCGTGCTGATGGGACAATCACACTTACTGTGTTGTCAACTGATAGAAAGGCAGCCACAGTGAGGTGTCGCTGTGAAAATACGGCTGTCCTTGGCGAGAGAAAAAATGTTAATTTGCCTGGAGTTGTGGTTGATCTGCCGACTCTTACAGAAAAAGACAAGGAGGATATTTTGGTGTGGGGTGTTCCTAACAAAATAGACATGATTGCTCTTTCTTTTGTTCGAAAGGGCTCAGATCTTGTTAATGTTAGGCAAGTATTGGGGAAGCATTCAAAGAATATTATGCTGATGTCAAAGGTTGAGAATCAGGAAGGCGTGGTCAACTTCGATGAAATTTTGCGTGAGTCTGATGCATTTATGGTTGCTCGTGGAGATCTTGGTATGGAGATTCCAGTTGAAAAAATATTCTTGGCTCAGAAAATGATGATATACAAGTGCAATCTTGTCGGAAAGCCTGTTGTCACTGCTACTCAAATGTTAGAATCGATGATTAAGTCTCCTCGCCCTACCCGTGCTGAAGCTACAGATGTGGCTAATGCTGTTCTAGATGGGACTGATTGTGTTATGCTTAGTGGTGAAAGTGCAGCAGGGGCATATCCTGAGGTGGCAGTGAAGACCATGGCTCACATATGTATAGAAGCTGAATCTTCGCTGGACTACTCAGCTATCTTTAAAGATTTAATAAGGTCTACTCCCTTACCTATGAGCCCCCTAGAGAGTCTTGCATCTTCGGCTGTTCGAACTGCAAATAAAGCAAAGGCAACACTCATTGTAGTTCTAACTCGTGGTGGCACCACTGCTAAATTGGTAGCAAAGTACAGACCAGCTGTACCTATTCTCTCTGTTGTGGTTCCTGTCCTGACAACAGATTCATTTGATTGGACATGTAGTGATGAAACTCCTGCCAGGCACAGTCTCATATACAGAGGATTGGTCCCACTTCTGGCAGAAGGATCAGCAAAGGCAACTGATTCTGAATCTACTGAAGTGATTCTGGATGCTGCTCTTAAAACAGCAATGGCGAAAGGCTTATGTAAGAAGGGTGATGCAATTGTTGCGTTACACCGCATAGGAGTGGCCTCTGTTATCAAGATATGTGATGTGAAGTGAGGTAATTTATTGCAACGAGATAACATGTGCCAAGTCTTGGGTCGTGCTCTTCTGCTACTCTTCAGTGCATGTTTGGAGACTTTGGCCAACCAGAAGATCATTTTGCAAGTTTAGATATGCTTAGTTGAAAACACTGCAGTTCTCAGTTTTGTCAAGTTTTGGGATTAGGAATTTCTCTTCATATTTTGGTATTATTAATTTTCAATTGTTAAAGATATTGAAGCAATGTTCTAATCTAGTTTCTACCCCCAAGAAATAAATGTTTGCAAAATAGCCCAAAAAAGTAAATATACGACTGGATATATTTGAATAAGAAACCTCACAATGTGGTGGTTTTTATTAATAAGAGTTTACGTATGCATT

Ace file of the UniGene sp_v1.1_unigene20901

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pyruvate kinase n=3 Tax=Spermatophyta RepID=B8LL81_PICSI	-	-	95%
FL-Next	sp=Pyruvate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	pyruvate kinase	-	-	93%

Source	GOs	Term	Type	Identity
Blast2go	response to cadmium ion	GO:0046686	Biological Process	93%
Blast2go	transferase activity	GO:0016740	Molecular Function	93%
Blast2go	plasma membrane	GO:0005886	Cellular Component	93%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LR82

Fln msg:

Fln protein: MANIDIEGILKEIPNDGRVPKTKIVCTLGPASRSVEMIEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMYNTQIMCAVMLDTKGPEIRTGFLKDAKAIQLREGQEITITTDYTIKGDTNMISMSYKKLAEDLSPGNTILRADGTITLTVLSTDRKAATVRCRCENTAVLGERKNVNLPGVVVDLPTLTEKDKEDILVWGVPNKIDMIALSFVRKGSDLVNVRQVLGKHSKNIMLMSKVENQEGVVNFDEILRESDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEVAVKTMAHICIEAESSLDYSAIFKDLIRSTPLPMSPLESLASSAVRTANKAKATLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSDETPARHSLIYRGLVPLLAEGSAKATDSESTEVILDAALKTAMAKGLCKKGDAIVALHRIGVASVIKICDVK*

Protein Length: 512

Fln nts: CGTCGTGACCTTGGCTGTCACTCAACATTGGTTTTGTTTGGCATAGCCAATATAAAGGAGTCGAAGAAGGTTTGGTGTTAAGGATTGTAAGGGATACCTACTCGCCTATTCATTGTTCCGGGTAGCTGCAACTGTGGTTCGAACCTAGAACGTTAGCTAGAGGCAAGCCGTCTGTATTAGGCCGGGAATTATTGAAAAGTTCGGCCTTGAACGACTCTCTGAAGCTGCGGATCCATTTCTTGGTGAGGATCAGAGTTAATTGTCCTCGTCTAATCTTTTTGCGAATCCAAGCTGCTGTAGATTCATCTTAGGTTTTGTA_-_ATGGCCAACATCGACATTGAGGGTATTTTGAAGGAAATTCCCAATGATGGGAGGGTACCAAAGACCAAGATTGTGTGTACACTGGGCCCTGCCTCTAGGAGTGTTGAGATGATCGAGAAGCTGCTGAGGGCTGGGATGAATGTTGCCCGATTCAACTTCTCTCACGGTTCACATGAGTATCACCAAGAGACGCTTGATAATCTCAGGACAGCGATGTATAATACGCAGATAATGTGTGCTGTTATGCTTGATACCAAGGGGCCTGAGATTCGAACTGGATTTTTGAAGGATGCAAAAGCAATCCAACTTAGGGAAGGTCAGGAAATCACAATAACTACAGATTACACTATTAAGGGTGATACTAACATGATCTCCATGAGCTATAAAAAACTGGCAGAAGATTTAAGTCCTGGTAATACAATCTTGCGTGCTGATGGGACAATCACACTTACTGTGTTGTCAACTGATAGAAAGGCAGCCACAGTGAGGTGTCGCTGTGAAAATACGGCTGTCCTTGGCGAGAGAAAAAATGTTAATTTGCCTGGAGTTGTGGTTGATCTGCCGACTCTTACAGAAAAAGACAAGGAGGATATTTTGGTGTGGGGTGTTCCTAACAAAATAGACATGATTGCTCTTTCTTTTGTTCGAAAGGGCTCAGATCTTGTTAATGTTAGGCAAGTATTGGGGAAGCATTCAAAGAATATTATGCTGATGTCAAAGGTTGAGAATCAGGAAGGCGTGGTCAACTTCGATGAAATTTTGCGTGAGTCTGATGCATTTATGGTTGCTCGTGGAGATCTTGGTATGGAGATTCCAGTTGAAAAAATATTCTTGGCTCAGAAAATGATGATATACAAGTGCAATCTTGTCGGAAAGCCTGTTGTCACTGCTACTCAAATGTTAGAATCGATGATTAAGTCTCCTCGCCCTACCCGTGCTGAAGCTACAGATGTGGCTAATGCTGTTCTAGATGGGACTGATTGTGTTATGCTTAGTGGTGAAAGTGCAGCAGGGGCATATCCTGAGGTGGCAGTGAAGACCATGGCTCACATATGTATAGAAGCTGAATCTTCGCTGGACTACTCAGCTATCTTTAAAGATTTAATAAGGTCTACTCCCTTACCTATGAGCCCCCTAGAGAGTCTTGCATCTTCGGCTGTTCGAACTGCAAATAAAGCAAAGGCAACACTCATTGTAGTTCTAACTCGTGGTGGCACCACTGCTAAATTGGTAGCAAAGTACAGACCAGCTGTACCTATTCTCTCTGTTGTGGTTCCTGTCCTGACAACAGATTCATTTGATTGGACATGTAGTGATGAAACTCCTGCCAGGCACAGTCTCATATACAGAGGATTGGTCCCACTTCTGGCAGAAGGATCAGCAAAGGCAACTGATTCTGAATCTACTGAAGTGATTCTGGATGCTGCTCTTAAAACAGCAATGGCGAAAGGCTTATGTAAGAAGGGTGATGCAATTGTTGCGTTACACCGCATAGGAGTGGCCTCTGTTATCAAGATATGTGATGTGAAGTGA___GGTAATTTATTGCAACGAGATAACATGTGCCAAGTCTTGGGTCGTGCTCTTCTGCTACTCTTCAGTGCATGTTTGGAGACTTTGGCCAACCAGAAGATCATTTTGCAAGTTTAGATATGCTTAGTTGAAAACACTGCAGTTCTCAGTTTTGTCAAGTTTTGGGATTAGGAATTTCTCTTCATATTTTGGTATTATTAATTTTCAATTGTTAAAGATATTGAAGCAATGTTCTAATCTAGTTTCTACCCCCAAGAAATAAATGTTTGCAAAATAGCCCAAAAAAGTAAATATACGACTGGATATATTTGAATAAGAAACCTCACAATGTGGTGGTTTTTATTAATAAGAGTTTACGTATGCATT

Fln Alignment:

sp_v1.1_unigene20901___MANIDIEGILKEIPNDGRVPKTKIVCTLGPASRSVEMIEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMYNTQIMCAVMLDTKGPEIRTGFLKDAKAIQLREGQEITITTDYTIKGDTNMISMSYKKLAEDLSPGNTILRADGTITLTVLSTDRKAATVRCRCENTAVLGERKNVNLPGVVVDLPTLTEKDKEDILVWGVPNKIDMIALSFVRKGSDLVNVRQVLGKHSKNIMLMSKVENQEGVVNFDEILRESDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEVAVKTMAHICIEAESSLDYSAIFKDLIRSTPLPMSPLESLASSAVRTANKAKATLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSDETPARHSLIYRGLVPLLAEGSAKATDSESTEVILDAALKTAMAKGLCKKGDAIVALHRIGVASVIKICDVK
B8LR82_______________MANIDIEGILKEIPNDGRIPKTKIVCTLGPASRSVPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMYNTQIMCAVMLDTKGPEIRTGFLKDAKPIQFKEGQEITITTDYTIKGDANMISMSYKKLAEDLRPGNTILCADGTITLTVLSTDKKAATVRCRCENTSVLGERKNVNLPGVVVDLPTLTEKDKEDILVWGVPNKIDMIALSFVRKGSDLVHVRQVLGKHAKNIMLMSKVENQEGVVNFDEILRESDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLLGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPESAVKIMAHICIEAESSLDYGAIFKELIRSTPLPMSPLESLASSAVRTANKAKATLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSDETPARHSLIYRGLIPLLAEGSAKATDSESTEVILDAALKTAIAKGLCKKGDAIVALHRIGVASVIKICDVK

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
47	66		33		0.997452
49		66	33		0.997452
50		66		33	0.997452
239		66		33	0.997452
240	66			33	0.997452
241	66	33			0.997452
280			33	66	0.997452
281		33		66	0.997452
282	33			66	0.997452
284		66		33	0.997452
287	83		16		0.994512
289	16	83			0.994512
292	83		16		0.994512
295		16		83	0.994512
498	4		95		0.995055
1703		87	12		0.992554
1704	87		12		0.992554
2101	5	82	11		0.999919
2139			33	66	1.0
2179	75			25	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain