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Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ADA3

Fln msg: Separated hits, possible frame ERROR between 218 and 220, query STOP codon too far from subject stop. Distance to subject end: 69 aas, putative chimeric sequence, atg_distance in limit (1-15): atg_distance = 2, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 97 - 214

Fln protein: MRVQLRNQPPYAARVEKIESGAKKNVEVGVRWSYRPDSKGRRxxDLFLSPHYDKQSMKNIEDTCVVHSLNHFCRFEYTATIGAFIHERSMIKRQRR*

Protein Length: 98

Fln nts: CTGTCGGAGTATATCATCAGGTATGATATTCCCTATCTAAATCGACCTTGGCTGCGGTCGGTTTTTAAACGGTGGGAGACTCAGTGCTC_-_ATGCGCGTTCAGCTTCGGAATCAGCCGCCTTACGCAGCGCGGGTGGAGAAGATTGAAAGTGGCGCGAAAAAGAATGTGGAGGTTGGAGTCCGGTGGTCTTACAGGCCGGACTCAAAGGGGCGGCGGCAxxxTGACCTCTTCTTGTCTCCTCACTACGACAAGCAGAGCATGAAAAACATTGAGGATACCTGCGTAGTCCACAGCTTGAACCATTTCTGTCGCTTCGAATACACAGCCACCATTGGCGCCTTCATTCACGAGCGGTCAATGATAAAACGACAAAGGAGGTGA___ATTACCATGTGGCAATTCATCCTGTGCAGGACAGGATCTGTATGATGACTGGGACTGATATTGGCACATTGTTTCAACTAAAGTTTTCCTGAAGGTGTTATTGATTTGTTTGATGTCCAAGTTGTACTGTCCGAGTAGTTTTCAGTTTGGCTTCTTGGCTAATACTGTGTTATAGTGACGTTTGGGACCCTATTTTCACTGTCTGGGTATTAGGTTGTTGACATTGTGTTTCGTCAACTCTTAAATATGACGTTGTTTTGAGGTTTATCAATATCAATATGTAGAAGGTTCTGAGGATTCAGAGCGGTACCAGAGCTCCTGAGGTTACCAGGTAAGTCGAATAATTTTTAATAATGAAAAATCGTTGGTCATAGACA

Fln Alignment:

sp_v1.1_unigene20728___VGDSVLMRVQLRNQPPYAARVEKIESGAKKNVEVGVRWSYRP-DSK-GRRQxDLFLSPHYDKQSMKNIEDTCVVHSL------------NHFCRFEYTATIGAFIHER
D5ADA3_______________VGDSVLMRAQDPDEPPYVAQVEKFERGARNNVKVRVRWYYRPEDSKAGRRQxELFLSDHYDTQSVNTIEDTCVVHSFKNYSNLESVASEDYFCRFEYTPITGYFNPDR