SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene20471

Length: 1516 nt

Origin of reads: Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene20471

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	similar to non-symbiotic hemoglobin class 1	-	-	5.0e-24	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	88%
Blast2go	non-symbiotic hemoglobin class 1	-	-	6.75902e-34	78%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LND3

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 89 - 250

Fln protein: YCRLRESAIQLREKGTATFSESNVKDLGKLHFKYGVVDEHFDVVKFCLLETIKDAVPDIWSLEMKTAWDEAYTQLAEAIKSEMKTVQP*

Protein Length: 90

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGATTTTGGCTTTTTTGTAAATGGTATATCTTTCACAGACACAGAAGTAAAGAAACATGATATGCAGAGACAGAAACAGTTACAGCAAGAAGTATTATATTTTCAATAGTGCTTCTTAGATGCGGTGACAAAGACTCTTTCTGCATCTGAGAAGCACTCCTAAACATATATTAATTATTGTTGTAACTGTTTCTATTTTGCATGTTTCTTCACTACATTACCTGTGCAATATATACACTGTACGAAAAAAATCGCATGGATCTATTGCACAGACACAGACACAGCAGTAAAGAAATAAAACCACAAACGAAAAGTTCAGGAGGGCTTCGTACATGCAGAGACAAAGTCATTCTCTGCATCTAAGAATCACTGCAGCAAGAACAAATATATGTTCAACAGTGCTTCAGAGTCTGTCACTGCACCTAAGAAGCTTTGTTGAAAGGATGCTAATTCTTAGTAATTGTTTATGTCTCTGCATTTGTTTCTTCACTACTGTATCCTTGTAGTATATATTTACCTTTGTTGGGATTGTGTTTCTTTTTTTTCACATTTCAGTCATGGCATATGACTGGTTCTGATCAACTTTAAGTAGATATAGACCCGAATGTAGAATACAACAACACGTACTCTTCTCTCTGGTATAAAGTAGGGATAACAACATAAAATCTTCATGAACCTTACTGTT_-_ATGATATTGCAGACTTCGTGAATCTGCCATTCAACTACGAGAGAAAGGAACGGCAACATTCTCAGAATCAAACGTCAAGGACTTGGGGAAGCTTCATTTTAAATATGGAGTAGTTGATGAACATTTTGATGTTGTTAAATTCTGCTTGCTCGAGACTATTAAGGATGCTGTTCCGGACATATGGTCTCTAGAAATGAAAACTGCCTGGGATGAAGCCTATACCCAATTGGCTGAAGCTATCAAAAGCGAAATGAAGACTGTGCAACCATGAGTGCCCTGATTTTGTTGCTGAGTATTGACAAAGTCTTGGCAACTAATCCCTCGGCGGTATTTAAGATATTGGATTATGACGTTTCAAGAGATGATAAAAGTCTATTTCTTGATAACCTGAAGAACTTGTGGAAAGATATTATTAGTTGTTCATAACCAGTAATAGACCTCAATGTCTGAAGATGGTTGTCACTGAGATTTATGAGAAGTAATTAAGAACCCACATAACCATAATTGGCTCCTATCAGCTATACATAACGCCCTCGGTCATTGTTTTAAGATTCCTTCGTAGATTTTACACCTGTTATAGAGTCCTGAAAGTAAGGAGGTACCTTTGCGCCCAGATTTCCATAAATTGCATCAGATTCAATTTTTGGGTAAAACCCCTGTAGATTGTAAAGAGCTTTACTCACATAGATTGGGTTAATAATCTTATTGTCCTTAATTACAGTTCTTTGAACAATCATGTCACGGAATATTGGGCTGGTAATGCTATTGTTCTTAGTTATTTGAACTATTTTCCATCATATGAATTTAT

Fln Alignment:

sp_v1.1_unigene20471___ESAIQLREKGTATFSESNVKDLGKLHFKYGVVDEHFDVVKFCLLETIKDAVPDIWSLEMKTAWDEAYTQLAEAIKSEMKTV
B8LND3_______________ESAVQLREKGTVTFSESNAKDMGKLHLTYGVLDEHFDVVKFCLLETIKDAVPDIWSPEMKIAWDEAYTQLAEAIKSEMKTV

SNPs (tot: 46)

Position	A %	C %	G %	T %	Probability
79		66		33	0.997452
540	33	66			0.997452
596		20	80		0.995493
604		60		40	1.0
607	50		50		1.0
630	33	66			1.0
636	33		66		1.0
652		50		50	1.0
690		25	25	50	0.995533
705		50		50	1.0
716			33	66	0.997452
742		66		33	0.997452
743	66		33		0.997452
766		66	33		0.997452
788		66		33	0.997452
798	33			66	0.997452
876	75		25		0.996473
944	25		75		0.996473
997	75			25	0.996473
1114	83		16		0.999991
1144	94	2			0.967557
1145			96	4	0.99411
1147	96		4		0.99411
1148	96		4		0.99411
1149		4		96	0.99411
1150	96			3	0.993591
1152	96			3	0.993591
1154	37	62			1.0
1156		62	37		1.0
1171	22		78		1.0
1178		52		47	1.0
1208	58			41	1.0
1209	26	74			1.0
1251		25	75		1.0
1261		39		60	1.0
1268		22		77	1.0
1287	15	85			1.0
1306	35		64		1.0
1310		21	78		1.0
1318	22	77			1.0
1324	23		76		1.0
1325		76	23		1.0
1335		87		12	1.0
1385	66			33	1.0
1403	47		52		1.0
1477		11	88		0.923564

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain