SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene19834

Length: 1515 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene19834

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) n=1 Tax=Vitis vinifera RepID=D7T9J5_VITVI	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	27%
Blast2go	protein	-	-	77%

Source	ECs	Term	Type	e value	Identity
Blast2go	Phosphatidylcholine--sterol O-acyltransferase.	EC:2.3.1.43	-	0.0	77%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Glycerophospholipid metabolism	00564		0.0	77%

Source	GOs	Term	Type	e value	Identity
Blast2go	lipid metabolic process	GO:0006629	Biological Process	0.0	77%
Blast2go	phosphatidylcholine-sterol O-acyltransferase activity	GO:0004607	Molecular Function	0.0	77%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase	IPR003386	-	0.0	77%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQP7

Fln msg: Distance to subject end: 254 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 338 - 681

Fln protein: MDTVWREILEKIEEWLHLAEKKQPYVNPDLDPVLLVPGIGGSILNAVNEKGHNERVWVRLFAADHEFRTKLWSLYDPSTGKTMSLDPKTKIEVPEDRFGLYSCDILDPDLIIRLNTVYYFHDMIGKMLGWGYQEGTTLFGFGYDFRQSNRLQETMDRLRAKLEFIHKASGGKKVTIISHSMGGLLIKCFLSLYNEVFEKYVNTWIAIAAPFEGAPGFIMDVLLNGVAFLEGWQQDLFISKWSMHQLLVECPSIYELMACPDFKWSSPPLLEIWRKKNDSFGHSSVGLECYGPHNYVDVMKDALVNNTVIYGGVTIPLPFNCEILKWANETRKIMSLAK

Protein Length: 339

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATCTTCACAAATCCGAGGATTATTCATTACGTGTAGAAAATAAAGTGATTGACGTTGAAAGATTTCCACATAAGGAATCTCTCTATTTATGTCTGATCCATCACGGATTTGGGGTTTGCCTGATCATGGCTGGCGCTTCCTTAGATTCTTGAGGAATTCGTAATTGACGCTGGAATCGGTGGATTATGCATTCCTTGGTAGGATATTAGATTGGGCTGGGCTTTTCACGCAGGCAGGAATTCATAATCGGCGCTGCAAGCGGTGGATTATGCATTCCTCGGCAGGACATTAAATTGGGCTTTTCACGCAGGAGCCAACAATTATTGCTGGGTCCCCGTCTTCCATCGTCGCTCACAGAGGTATTATCTTTCAGAAAGGGGCTTTATCCTGGAAACATCGTGCATAACGTCTTTAAGTAGAAAGAATTCCTTTCTCTCCTGTGATACAGAATCTACTTTGACATTGGTACAGTGAAA_-_ATGGACACAGTATGGCGGGAAATACTTGAAAAAATTGAAGAGTGGCTTCACTTGGCTGAGAAAAAGCAACCATACGTGAATCCTGATTTGGACCCAGTTTTACTTGTGCCTGGCATAGGGGGTTCGATATTAAACGCCGTGAATGAAAAGGGCCACAACGAGCGAGTTTGGGTTCGTCTTTTTGCCGCTGATCATGAATTTCGAACAAAGCTTTGGTCCCTTTATGATCCTAGCACTGGAAAAACTATGTCCTTGGATCCAAAAACAAAGATTGAAGTCCCGGAAGACAGATTTGGACTATATTCTTGTGACATTCTGGACCCTGATCTGATTATTCGGCTGAATACTGTTTACTACTTCCACGACATGATAGGAAAAATGCTAGGTTGGGGATATCAAGAGGGTACTACTCTCTTTGGTTTTGGCTATGACTTTCGACAAAGTAACAGGCTTCAAGAAACAATGGATCGTTTAAGAGCAAAGTTAGAATTCATACATAAGGCCTCTGGAGGAAAGAAAGTAACCATCATAAGCCATTCAATGGGAGGTCTTCTAATCAAATGTTTTCTCTCACTCTATAATGAAGTATTTGAGAAGTATGTGAACACGTGGATAGCCATTGCTGCACCGTTTGAAGGTGCACCTGGATTTATAATGGACGTTCTTTTGAATGGAGTTGCATTTCTTGAAGGATGGCAACAAGATCTTTTCATTTCTAAGTGGAGCATGCATCAACTGTTGGTGGAATGCCCCTCAATATATGAGTTAATGGCCTGCCCTGATTTCAAGTGGTCTTCACCTCCTCTTCTGGAAATATGGCGAAAAAAGAACGACAGCTTTGGCCACTCATCAGTTGGACTTGAGTGCTACGGTCCACACAACTATGTTGATGTCATGAAGGATGCTCTTGTTAATAACACCGTTATCTATGGTGGTGTGACAATACCATTGCCATTTAATTGTGAAATATTGAAATGGGCCAATGAAACTCGAAAAATCATGTCCTTGGCGAAGA

Fln Alignment:

sp_v1.1_unigene19834___PVLLVPGIGGSILNAVNEKGHNERVWVRLFAADHEFRTKLWSLYDPSTGKT-------MSLDPKTKIEVPEDRF----GLYSCDILDPDLIIRLNTVYYFHDMIGKMLGWGYQEGTTLFGFGYDFRQSNRLQETMD----RLRAKLEFIHKASGGKKVTIISHSMGGLLIKCFLSLYN-----------EVFEKYVNTWIAIAAPFEGAP
B8LQP7_______________PVVFVPGIVTGGLE----------LWEGRPCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNDTGLDPPGIRLRPVSGLVAADYFAPGYFV-------WAVLIANLAQIGYEE-KTMHMAAYDWRLSFQNTEVRDQALSRLKSNIELMVKTNNGRKVVAIPHSMGALYFLHFMKWVEAPAPMGGGGGPDWCAKYLKAVMNIGGPFLGVP

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
118	20		20	60	0.99255
119		20	80		0.995493
123		80	20		0.995493
124		80	20		0.995493
166	60			40	1.0
179		60	40		1.0
223	60		40		1.0
238			25	75	0.996473
664		66	33		0.997452
665		33	66		0.997452
670		33		66	0.997452
671	33			66	0.997452
672		33	66		0.997452
673			66	33	0.997452
674		33	66		0.997452
771	33	66			1.0
922		33		66	1.0
1047	40		60		1.0
1049	60			40	1.0
1186		14		85	0.993533
1187			14	85	0.993533
1190			14	85	0.993533
1194	85	14			0.993533
1195		14	85		0.993533
1196			85	14	0.993533
1202	85			14	0.993533
1203	85		14		0.993533
1204		83		16	0.994512
1205	83	16			0.994512
1207		16	83		0.994512
1383		25		75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain