SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene19454

Length: 1404 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene19454

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	oxidoreductase, 2OG-Fe(II) oxygenase family protein; K00472 prolyl 4-hydroxylase [EC:1.14.11.2]	-	-	76%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	72%
Blast2go	2og-fe oxygenase family protein	-	-	83%

Source	ECs	Term	Type	e value	Identity
Blast2go	Procollagen-proline dioxygenase.	EC:1.14.11.2	-	0.0	83%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Arginine and proline metabolism	00330		0.0	83%
Blast2go	Metabolic pathways	01100		0.0	83%

Source	ECs	Term	Type	e value	Identity
AutoFact	Procollagen-proline dioxygenase.	EC:1.14.11.2	-	0.0	76%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Arginine and proline metabolism	00330		0.0	76%
AutoFact	Metabolic pathways	01100		0.0	76%

Source	GOs	Term	Type	Identity
Blast2go	peptidyl-proline hydroxylation to 4-hydroxy-L-proline	GO:0018401	Biological Process	83%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	83%
Blast2go	L-ascorbic acid binding	GO:0031418	Molecular Function	83%
Blast2go	iron ion binding	GO:0005506	Molecular Function	83%
Blast2go	procollagen-proline 4-dioxygenase activity	GO:0004656	Molecular Function	83%
Blast2go	Golgi apparatus	GO:0005794	Cellular Component	83%
Blast2go	plasma membrane	GO:0005886	Cellular Component	83%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9E9

Fln msg: Overlapping hits, possible frame ERROR between 516 and 486,

Fln protein: MHRNFPYYKLVQPMALTQPELFSCLGxxxxxxxxxxxxDAECDHLMFLARDKLQKSMVADNESGKSVQSEIRTSSGMFLNKAQDEIVANIEDRIAAWTFLPKENGEAMQVLHYELGQKYEPHFDFFHDKINQAMGGHRIATVLMYLSDVVKGGETVFPNSEAKDSQPKDDSWSECGKSGYSVKPSKGDALLFFSLRPGATTDPSSLHGSCPVIEGEKWSATKWIHVRSFDAPIRQVSGECIDENSSCAHWASIGECKKNPLYMVGSPDLPGSCRKSCQVC*

Protein Length: 282

Fln nts: ATGGGGACGCGCTACCTTCTAATTTCAATACATAAGTTTGACACGTCCGACCATTTCCATGGTGTCTTCATCCTGTGACTGAGGACTTAACTCTCTATCATTAATAAATCCATAAATGTTATACGCGTGTATGCGATTCTGGGGTTTTGATTCCGTCGAGAGCAGACAGGATTGGTGTCCATTCAAAGCTCTCGTTGCTGCATTTGGGTATTTACCAGACTGAATTTCACAGGACGTAAAATTTTCTTTATGCTGGGAACGAAGAAATTCCGAATGCCCATGGCGCCATTGGTAGCACTTTCAATAGTTCTTGCTTGGCATTCTATGGACGAGTGGCTGACTCCGCAATGGATCTGCCGGGGTGGGTTGGTGAAACGTACAGAAAGCCATTGAGAACTACAAAGG_-_ATGCATCGGAATTTTCCATATTACAAACTGGTCCAACCTATGGCTTTGACCCAACCCGAGTTATTCAGTTGTCTTGGCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGATGCAGAGTGTGACCATCTTATGTTCTTGGCAAGGGACAAGTTACAGAAATCTATGGTTGCAGACAATGAATCGGGGAAAAGTGTTCAGAGTGAGATCAGAACAAGCTCAGGAATGTTTTTGAATAAAGCTCAGGACGAAATTGTTGCAAACATTGAGGATAGGATAGCAGCTTGGACTTTTCTTCCAAAAGAAAATGGCGAGGCTATGCAGGTTTTGCATTATGAACTTGGCCAGAAATATGAGCCTCATTTTGATTTTTTCCATGATAAAATTAATCAAGCCATGGGGGGCCATAGGATTGCTACAGTGTTAATGTATCTATCTGATGTTGTGAAGGGTGGTGAGACTGTCTTTCCTAACTCAGAGGCAAAGGACAGTCAACCCAAAGATGACTCTTGGTCAGAATGTGGCAAGAGTGGATATTCAGTGAAACCTAGCAAGGGAGACGCCCTACTTTTCTTCAGTCTTCGTCCAGGTGCTACAACTGATCCATCAAGTCTGCATGGTAGCTGCCCCGTTATTGAGGGGGAAAAATGGTCTGCAACAAAATGGATACATGTTAGGTCATTTGATGCTCCTATCAGACAAGTTTCAGGGGAGTGTATTGATGAAAATTCTAGTTGTGCTCATTGGGCTTCTATTGGGGAATGCAAGAAGAATCCTCTCTATATGGTTGGTAGTCCAGATTTGCCTGGTTCATGTAGAAAAAGTTGTCAAGTATGCTGA___CATTTTTTTTGAAGTTGAGCATATTGCCGGCTGTTTTTCAAAAATGCGATTTGACAGATATAATGTGACGGATTAAATTAAAATGTAATCTTCTCATATTTTGTTATCCACTAGCCCTTTTCTTCGTGATTTTTGATGTGTTGGTAAATGTTCTTAA

Fln Alignment:

sp_v1.1_unigene19454___MHRNFPYYKLVQPMALTQPELFSCLGVxxxxxxxxxxTDAECDHLMFLARDKLQKSMVADNESGKSVQSEIRTSSGMFLNKAQDEIVANIEDRIAAWTFLPKENGEAMQVLHYELGQKYEPHFDFFHDKINQAMGGHRIATVLMYLSDVVKGGETVFPNSEAKDSQPKDDSWSECGKSGYSVKPSKGDALLFFSLRPGATTDPSSLHGSCPVIEGEKWSATKWIHVRSFDAPIRQVSGECIDENSSCAHWASIGECKKNPLYMVGSPDLPGSCRKSCQVC
D5A9E9_______________MHRNFPYYKLVQLLALTRLELLSCLGIxxxxxxxxxxTDAECDHLIFLARDKLQKSMVADNESGKSVMSEIRTSSGMFLNKAQDEIVASVEDRIAAWTFLPIENGEAMQVLHYELGQKYEPHFDYFHDKINQAMGGHRIATVLMYLSDVVKGGETVFPNAETKDSQPKDDSWSECAKGGYSVKPNKGDALLFFSLRPDATTDQSSLHGSCPVIEGEKWSATKWIHVRSFEVSNRKISEGCVDENDSCTHWASIGECKKNPTYMVGSPDSPGACRKSCQVC

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
35	75	25			0.996473
39			25	75	0.996473
46			75	25	0.996473
49		75	25		0.996473
51			75	25	0.996473
52	75		25		0.996473
53		75		25	0.996473
57		11		88	0.923564
60	11	88			0.923564
566		12		87	0.999964
567	87		12		0.999964
568		87		12	0.999964
569	87	12			0.999964
571	87			12	0.999964
572	87		12		0.999964
574		12		87	0.999964
581		12		87	0.999964
586		12	87		0.999964
587	87			12	0.999964
1383		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain