SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene19412

Length: 1811 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene19412

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Acyl-protein thioesterase, putative (EC:3.1.1.1); K06130 lysophospholipase II [EC:3.1.1.5]	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	acyl-protein thioesterase 2	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on ester bonds, Carboxylic ester hydrolases.	EC:3.1.1.-	-	0.0	86%

Source	KEGGs	Term	Type	Identity
Blast2go	Ascorbate and aldarate metabolism	00053		86%
Blast2go	Bisphenol degradation	00363		86%
Blast2go	Glycerophospholipid metabolism	00564		86%
Blast2go	Toluene degradation	00623		86%
Blast2go	Aminobenzoate degradation	00627		86%
Blast2go	Retinol metabolism	00830		86%
Blast2go	Tropane, piperidine and pyridine alkaloid biosynthesis	00960		86%
Blast2go	Carboxylesterase.	EC:3.1.1.1	-	86%

Source	KEGGs	Term	Identity
Blast2go	Tropane, piperidine and pyridine alkaloid biosynthesis	00960	86%
Blast2go	Drug metabolism - other enzymes	00983	86%
Blast2go	Metabolic pathways	01100	86%
Blast2go	Biosynthesis of secondary metabolites	01110	86%

Source	ECs	Term	Type	e value	Identity
AutoFact	Lysophospholipase.	EC:3.1.1.5	-	0.0	73%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Glycerophospholipid metabolism	00564		0.0	73%

Source	GOs	Term	Type	e value	Identity
Blast2go	carboxylesterase activity	GO:0004091	Molecular Function	0.0	86%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Phospholipase/carboxylesterase/thioesterase	IPR003140	-	0.0	86%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKX3

Fln msg:

Fln protein: MSSGSTSNFSSGSRSQRRTFEYGRTHVVKPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVGDISEDAPADLEGLDSSAAHVANLLSTEPAEIKLGVGGFSMGAAISLYSATCCVYGKYSNGDPYPVNISAAVGLSGWLPSAKDLQNKLRESQEAVKRAQTMPIFMCHGKADDVVIYKHGEKSAQVLEASGFGNLTFKSYKGLGHYTIPEETEDVCRWLSVNLGLEGTRS*

Protein Length: 260

Fln nts: ATGGGATAAATTAAAATTCAACAACAATCTCATGTTGATGGTTTAATGTTTACCATGACTTTGACATATTCCTCTACCATATTTGTCCCAACTTCCATCTTTATTGGAGGAGGGTTTTGATCTTCCATCTTTATTGGAGGAGGGTTTTAATTTTGATCAAAACCCACGATGCTCCTCAGCGCACATTTAGTTGGTGGTGGGAGCTATCAGCTTTATGCGCTGATTCCCTGGGAGCTTGTGAAAAACGAGTCGTTTTCAGTCCCGAAGTGGCTCTGAAATCGAACAAACCCTCTTCGATTCGGACTAGATTTGTAACAAATTTAAAGCTATTTATATAGTTCTTGGTTTTGCGTTTTGTAAATCAGATCAGGTCTGTATTATCTCAGGTGGGCAAGAAGAGCTATTGTTCGCTGTTCAGTACGGGAAATTATATCCGGAAATTCACA_-_ATGAGTAGCGGAAGTACCTCCAATTTTAGCTCTGGAAGTAGGTCACAAAGGCGCACATTTGAGTATGGGAGAACACATGTAGTCAAACCAAAGGGGAAACATCAGGCCACCATTGTGTGGCTTCATGGGCTCGGTGACAATGGTTCAAGTTGGTCCCAGCTCTTGGAAACGCTTCCTCTTCCTAATATAAAATGGATATGCCCAACAGCTCCTACTCGCCCAATAGCTCTTTTTGGTGGTTTTCCCTCCACTGCTTGGTTTGATGTTGGTGACATTTCTGAAGATGCTCCTGCTGACTTGGAAGGCTTAGATTCTTCAGCAGCACATGTAGCAAACCTATTATCAACCGAGCCCGCTGAAATCAAACTTGGTGTTGGAGGTTTCAGTATGGGTGCAGCAATTTCCTTGTATTCAGCAACTTGTTGTGTTTATGGGAAGTACTCTAATGGCGATCCATATCCAGTTAATATTAGTGCAGCTGTTGGCTTAAGTGGGTGGCTTCCTTCTGCCAAGGACTTACAGAACAAGTTACGAGAGTCTCAGGAGGCTGTAAAGCGTGCACAAACGATGCCTATTTTTATGTGTCATGGTAAAGCGGATGATGTTGTCATTTACAAACATGGAGAAAAGTCTGCTCAAGTTTTGGAGGCCTCTGGATTTGGCAATTTGACTTTCAAAAGTTACAAAGGGTTGGGTCATTATACAATTCCTGAAGAAACGGAGGATGTTTGTCGATGGTTGTCAGTAAACCTAGGACTTGAGGGCACACGCTCTTAA___TTACAAAACCTATCTGCAAATAAGGATAGATGTGGAAACATGCGATTTGATTCAAGCATATCGATGTTCACATGAGAGTCAAGGTTCATTCAAAGGGTTTATAATTCGGGGCAAGCAACTTTAGATCTGTGATTCATTTTCATATGTAATTAAAGGGTATTATGGTGAGAGCTTTACCAGGTGCATCTGAATTTTTTGATTGTTCACAAGAATCTGCCGCTGGCTGATCAACTCATTATATTTTCTAATGCTAACAAGGATTCTTGGTTTGCGTTCAAATTTATAACTCCAAATTATGATGTGAAATTAATTGGTGTGGAGATGTGGTGGAAACTTAAGTTAATTTTTAAGATTTTGAGTACAAACAACTCTGCATTCTATTTTTTGGCCCTCGAGTGCATCCGACAACAGTTGGATGCTATAGTATAGTTGGGAGAAGCAGCGTTAAGATATGATCTGAGGAAAGCTAAGCTTTCATTATGTGCTGTTATCTACTACATAATTTTTTCCATCTCACACATTTTCCCTCTTATTTTTCTGCTGAATAGGTGACAGATTCTGACAAGTCTTACATATCTAGTCACGTGG

Fln Alignment:

sp_v1.1_unigene19412___QRRTFEYGRTHVVKPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVGDISEDAPADLEGLDSSAAHVANLLSTEPAEIKLGVGGFSMGAAISLYSATCCVYGKYSNGDPYPVNISAAVGLSGWLPSAKDLQNKLRESQEAVKRAQTMPIFMCHGKADDVVIYKHGEKSAQVLEASGFGNLTFKSYKGLGHYTIPEETEDVCRWLSVNLGLEGTRS
B8LKX3_______________QRRTFEYGRTHVVKPKGKHQATIVWLHGLGDNGSSWSQLLEMLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVGDLSEDAPADLEGLDSSAAHVANLLSTEPAEIKLGVGGFSMGAAISLYSATCCVHGKYSNGDPYLVDISAAVGLSGWLPCAKDLQNKLRVSQEAVKRAQTMPLLLCHGKVDDVVIYKHGEKSAQALEASGFSNMTFKSYKGLGHYTIPEEMEDVCRWLSVNLGLEGTRS

SNPs (tot: 54)

Position	A %	C %	G %	T %	Probability
52		50		50	1.0
53	50		50		1.0
54		50		50	1.0
56	50		50		1.0
61		50		50	1.0
65	50		50		1.0
69	50		50		1.0
71		50		50	1.0
72	50	50			1.0
76	50			50	1.0
80	50		50		1.0
166		75		25	0.996473
229		83	16		0.994512
232			83	16	0.994512
235		16	83		0.994512
242	83			16	0.994512
243	83	16			0.994512
245	83	16			0.994512
246	83			16	0.994512
253	16		83		0.994512
333		11		88	0.923564
334	88		11		0.923564
336	88	11			0.923564
337			11	88	0.923564
338	88			11	0.923564
340	11			88	0.923564
341		11		88	0.923564
349		14		85	0.993533
351		14	85		0.993533
352	14	85			0.993533
795		12		87	0.992554
884	66			33	0.997452
886			33	66	0.997452
887	66		33		0.997452
1014	17		82		1.0
1030		3		96	0.986325
1034	96		3		0.986325
1037		3	96		0.986325
1038		3		96	0.986325
1039	96			3	0.986325
1040	96			3	0.986325
1041	96		3		0.986325
1044			96	3	0.986325
1048		3	96		0.986325
1119			17	82	1.0
1163	96		3		0.988113
1339			97	2	0.977151
1379			95	1	0.918242
1441	3	96			0.991825
1444		96		3	0.991825
1465			1	94	0.951749
1499		13		86	1.0
1650		11		88	0.923564
1787		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain