SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene19215

Length: 1083 nt

Origin of reads: Biogeco_1 Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene19215

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Cellulase domain containing protein	-	-	0.0001	22%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	50%
Blast2go	serine-threonine protein plant-	-	-	0.0	75%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Glycosylases, Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds.	EC:3.2.1.-	-	0.0	75%

Source	KEGGs	Term	Identity
Blast2go	Ascorbate and aldarate metabolism	00053	75%
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130	75%
Blast2go	Amino sugar and nucleotide sugar metabolism	00520	75%
Blast2go	Glycosaminoglycan degradation	00531	75%
Blast2go	Metabolic pathways	01100	75%

Source	GOs	Term	Type	Identity
Blast2go	primary metabolic process	GO:0044238	Biological Process	75%
Blast2go	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	75%
Blast2go	binding	GO:0005488	Molecular Function	75%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	0.0	75%
Blast2go	Glycoside hydrolase, superfamily	IPR017853	-	0.0	75%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A9Z9

Fln msg: Separated hits, possible frame ERROR between 655 and 799, Distance to subject end: 149 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 299 - 429

Fln protein: LSSASCSERKWVCEDKGHPLYCQWETSVCQWVQLLLVNDCSNGYNTEK*SYLCFQQAAAHRLNVARTWAFNDGQYNALQTFPGVYDEQVFQALDFVVFQARKYGIRLILSLANNYVNFERLAQYVQWARNAGQNLNSDDDFFTNPTVKAYYKNHVxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFLQTVLTRVNSITGVAYKDDPTIFAWELMNEPRCQSDPSGRTIQGWIEEMASHVKSIDGNHLLEAGFGRFLWRIQKRDQSRLLTKVGTDFITNNQ

Protein Length: 300

Fln nts: ATGGGGACCCTTCTCTAGTGTGCCAATGGGGCTCAAAGTTTTTTCCTGGTTAGCAGATCTGCTAGCTAATGCAGAGACGCTCCTTAAGTAGTTTTCACTTCTTTATGAAGTCTAGAGCTTTTTCCAATCAAGTGGGTGGTGCCATGAAGATGAGACTTTGGGTTCTTTCATGTCTGCTCTTTCTTAGCTTTCTTCAGCAAGCTGCAGCGAGAGGAAGTGGGTTTGTGAGGACAAGGGGCACCCACTTTACTGTCAATGGGAAACCTCTGTTTGTCAATGGGTTCAACTCTTACTGGTTAATGACTGTAGCAACGGATATAACACAGAGAAATAAAGTTACCTCTGTTTTCAACAAGCTGCAGCTCATAGGCTTAATGTTGCCAGGACTTGGGCTTTCAACGATGGCCAGTACAACGCATTGCAGACTTTTCCTGGTGTATATGATGAACAAGTATTTCAGGCTCTGGACTTTGTGGTCTTTCAAGCCAGAAAGTATGGTATCAGACTAATACTTAGTCTGGCAAACAATTATGTGAACTTTGAAAGATTGGCCCAATATGTACAATGGGCTCGCAATGCTGGACAGAATCTCAACTCTGATGATGATTTCTTCACCAATCCCACTGTTAAAGCCTATTATAAGAACCATGTTAAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTTCTTGCAGACTGTGTTGACTAGAGTGAACAGCATTACTGGAGTAGCCTACAAGGATGACCCTACAATCTTTGCATGGGAGCTCATGAACGAGCCTCGTTGTCAAAGTGATCCTTCTGGGAGGACCATCCAGGGATGGATAGAGGAGATGGCTAGCCATGTCAAATCCATCGATGGGAACCATCTTCTGGAGGCTGGTTTTGGAAGGTTTTTATGGAGAATACAAAAGAGAGATCAATCCCGGCTTCTAACTAAAGTGGGTACAGATTTCATCACCAATAACCAA

Fln Alignment:

sp_v1.1_unigene19215___DCSNGYNTEK*SYLCFQQAAAHRLNVARTWAFNDGQYNALQTFPGVYDEQVFQALDFVVFQARKYGIRLILSLANNYVNFERLAQYVQWARNAGQNL-NSDDDFFTNPTVKAYYKNHVKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFLQTVLTRVNSITGVAYKDDPTIFAWELMNEPRCQSDPSGRTIQGWIEEMASHVKSIDGNHLLEAGFGRFLWRIQ----KRDQSRLLTKVGTDFITNNQ
D5A9Z9_______________DQSVGDSTRHRVKTMLRRGAEMGLTVVRTWAFNDGGYNALQQFPGTFDERVLRALDFVIVEARRNGLRLIFSLVNNLNAYGGKVQYVEWAHAAGINVGSSNDSFFSDPTIRGYYKEYVKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYVKSILVRKNSISGVEYRNEPAIFAWELVNEPRCTSDASGQTLQTWISEMAKFIKSLDSRHLLTVGLEGFYGKTTPNKIELNPGDWAVSLGSDFIWNSQ

SNPs (tot: 3)

Position	A %	C %	G %	T %	Probability
770		33	66		0.997452
782			66	33	0.997452
808	25	75			0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain