SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene19181

Length: 3412 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene19181
CGTCGTGACCTTGGCTGTCACTCACTGGAACAGGGTTTCGATTCGCTACCTGTTTAGTGTCCAAGTATTTCCTCAATCCACGGCATCTTATTCTGCCCCTCACAGAATATTCGATCACCGTCCTGCTCCACCAAAAACCCTAACGGAGTTACGGTCTTCAACCAAAAGCCTACTCCATCGAGCAAAGTCCATCCTTTTGTTTGTCTCAGGCCGCCGGGTCATTCTTTTATCTTAGTACTCGCATGCCTTGCCTGAGCCGCGAGAGTCCGTGATTTGAAGGTTTCAGAGGTCTGCAACTCGAATCCATTCTGTGCCGTTCTCCTTTGGCGGGTGCTCATTGGTATTCTTCTGTTTTGGCTGAGAAGAGTTTTGGTGAATACAAAAGTTAGGAAAAATGGTGAAGTTCACGGCTGAAGAGCTTCGTAAAATCATGGACAGGAAGCATAACATACGAAACATGTCTGTTATTGCCCATGTTGATCACGGAAAATCCACTCTCACTGATTCTTTGGTAGCAGCAGCTGGTATCATTGCTCAAGAAGTTGCTGGAGATGTCAGAATGACTGATACTCGGGCAGATGAAGCTGAACGTGGCATCACCATCAAGTCAACTGGAATTTCGTTGTACTATGTGATGGAGGATTCAGCGGTGAAAAATTTCAAGGGGGATAGAGAAGGACACGAATATTTGATAAATCTGATTGATTCTCCAGGGCATGTTGACTTTTCTTCAGAAGTTACAGCAGCCCTGCGAATCACAGATGGTGCACTGGTTGTGGTAGATTGTATTGAAGGGGTTTGTGTCCAGACTGAGACTGTTCTTCGTCAAGCTCTTGGAGAAAGGATTAGGCCTGTTCTCACTGTGAACAAGATGGACAGGTGCTTTCTTGAGTTGCAGGTTGATGGAGAGGAAGCTTACCAAACCTTCTCACGTGTCATTGAAAATGCAAATGTGATTATGGCTACTTATGAAGATCCTCTTCTTGGTGACTGCCAAGTTTATCCTGAAAAAGGTACAGTTGCATTCTCGGCAGGTCTGCACGGGTGGGCATTTACCCTCACAAATTTTGCAAAAATGTATGCTTCAAAGTTTGGAGTGGATGAAGCAAAGATGATGGAGAGGCTCTGGGGTGAGAACTTTTTTGATCCAGCTACAAAGAAATGGACAACCAAGAATACAGGTTCTGCAACTTGCAAAAGAGGATTTGTTCAATTCATATATGAACCTATTAAGCAAATTATCAATATTTGTATGAATGATCAAAAGGATAAGTTGTGGCCCATGTTGCAGAAATTGGGAGTTGTAATGAAAAGTGACGAGAAGGAGCTGGTTGCAAAAGCACTCATGAAGCGTGTCATGCAAACCTGGCTACCTGCAAGTAGTGCTTTACTTGAAATGATGATTTTCCATCTTCCTTCTCCAGCGAAGGCACAGAGATATCGTGTGGAAAACTTGTATGAAGGACCTCTTGATGATGTATATGCAACTGCAATAAGAAACTGTGACCCTGAGGGTCCACTGATGTTGTATGTTTCAAAAATGATTCCAGCTTCGGACAAAGGACGTTTCTTTGCCTTTGGTCGTGTTTTTGCTGGCAGAGTTGCAACAGGTATGAAGGTAAGGATCATGGGTCCCAACTATGTGCCAGGTCAGAAGAAAGATTTATATACAAAGAGTGTTCAAAGGACTGTGATATGGATGGGAAAGAGACAAGAATCTGTTGAGGATGTGCCTTGTGGAAATACAGTGGCTTTGGTTGGGTTAGACCAGTTCATAACCAAAAATGCTACACTGACAAACGAGAAGGAGGCTGATGCTCATCCGATCAGAGCAATGAAGTTCTCTGTGTCCCCTGTTGTACGTGTTGCTGTGCAGTGCAAGGTGGCATCTGACCTTCCAAAACTTGTTGAAGGGCTGAAGCGTCTTGCTAAGTCGGATCCTATGGTTGTTTGTACAATTGAGGAATCTGGAGAACATATTATTGCAGGTGCAGGAGAGCTTCATCTCGAGATTTGCTTAAAAGATCTCCAAGATGATTTTATGGGTGGAGCTGAGATTGTTGTTTCTGATCCTGTTGTCTCATTTAGAGAAACTGTTTTGGAGAAATCATGCCGTACTGTTATGAGCAAGTCTCCAAATAAACATAACAGGCTTTACTTTGAAGCAAGACCTTTAGAAGAAGGTCTTGCAGAAGCAATTGATGATGGTCGCATTGGCCCAAAGGATGATCCAAAGGCTCGCGCAAAAATCCTTTCTGAGGAATTTGGATGGGACAAAGATTTGGCTAAGAAGATCTGGTGTTTTGGACCTGAAACCACTGGTCCCAATATGGTGGTGGATATGTGTAAGGGAGTTCAATACTTGAATGAAATTAAAGATTCTGTGGTTGCTGCATTCCAATGGGCATCTAAGGAAGGTGCTCTTGCAGAAGAAAATATGCGTGGTATTTGCTTTGAGGTTTGTGACGTGGTTTTGCACACTGATGCTATCCATAGAGGAGGTGGCCAGGTTATCCCAACGGCCAGAAGGGTTATGTATGCATCTCAGCTTACTGCCAAACCACGGCTTCTTGAGCCAGTATATCTCGTTGAGATTCAGGCTCCTGAGAATGCCCTTGGTGGTATTTATGGTGTGCTGAACCAGAAGAGAGGTCATGTTTTTGAAGAAATGCAGAGGCCAGGTACTCCACTATACAACATCAAGGCATATCTGCCAGTTGTTGAATCATTCGGATTCTCAAGCACTCTCCGTGCTGCAACTTCTGGCCAGGCTTTCCCTCAATGTGTGTTTGACCACTGGGATATGATGGGCTCTGATCCATTGGAACCTGGTTCCCAAGCTGGGCAGCTTGTGACTGATATCCGTAAGAGGAAGGGTCTTAAGGAGAGTATGACTCCCTTGTCAGAGTTCGAAGACAAGCTGTAGAGCTTTGCTATGTTTGCATGTCGGATGCTGTCAAGATTGAGGAACCTCCGAGTATTAAAACACAGTTTTGTGTGCTAGGACTAGTTGAATTTATGCTATTCAGGTATTTTTGTGATCTGTTATTTATGTTATTCAGGTATTTTTGTGATTTGAAAATACTTTTTACAAGGTACATCCATTAATCTTTTAAATGTTACATAATTCTCCCTTGTCAATTTCACCGTATGTGCGGTAGTTTGAATTGCTTGCATAATTATGTATGTCACAGAAAAGCCGAGCTGAGATTTGAGTAGACGTTGGTTTAAAAATTGTTGTATATATTTGTGCTCCATCTATTATTCTCCTCTCTAGTTTCCCAATCCATCCACGTAGTATTGTGCCTGTTGTGTCTTTTGACGTCAATGGGTTTTCCCTGTTAGTCTTATGTTATCCATCTGTGTCCAGTTCAAAGAGAAGCAAAACGAATCCACACGATAACCTGATTAA

Ace file of the UniGene sp_v1.1_unigene19181

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Elongation factor 2 n=16 Tax=Magnoliophyta RepID=EF2_BETVU	-	-	89%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	98%
Blast2go	protein	-	-	95%

Source	ECs	Term	Type	Identity
Blast2go	Protein-synthesizing GTPase.	EC:3.6.5.3	-	95%
Blast2go	Heterotrimeric G-protein GTPase.	EC:3.6.5.1	-	95%
Blast2go	Small monomeric GTPase.	EC:3.6.5.2	-	95%
Blast2go	Signal-recognition-particle GTPase.	EC:3.6.5.4	-	95%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	95%
Blast2go	translation	GO:0006412	Biological Process	95%
Blast2go	translation elongation factor activity	GO:0003746	Molecular Function	95%
Blast2go	GTP binding	GO:0005525	Molecular Function	95%
Blast2go	copper ion binding	GO:0005507	Molecular Function	95%
Blast2go	GTPase activity	GO:0003924	Molecular Function	95%
Blast2go	plasma membrane	GO:0005886	Cellular Component	95%
Blast2go	chloroplast	GO:0009507	Cellular Component	95%
Blast2go	cytosol	GO:0005829	Cellular Component	95%

Source	InterPros	Term	Type	Identity
Blast2go	Translation elongation factor EFG/EF2, C-terminal	IPR000640	-	95%
Blast2go	Protein synthesis factor, GTP-binding	IPR000795	-	95%
Blast2go	Translation elongation factor EFTu/EF1A, domain 2	IPR004161	-	95%
Blast2go	Small GTP-binding protein domain	IPR005225	-	95%
Blast2go	Translation elongation factor EFG/EF2, domain IV	IPR005517	-	95%
Blast2go	Translation elongation/initiation factor/Ribosomal, beta-barrel	IPR009000	-	95%
Blast2go	Elongation factor G/III/V	IPR009022	-	95%
Blast2go	Ribosomal protein S5 domain 2-type fold, subgroup	IPR014721	-	95%
Blast2go	Ribosomal protein S5 domain 2-type fold	IPR020568	-	95%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACX5

Fln msg: your sequence is longer than subject: 844 - 267

Fln protein: MVKFTAEELRKIMDRKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYVMEDSAVKNFKGDREGHEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSRVIENANVIMATYEDPLLGDCQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASKFGVDEAKMMERLWGENFFDPATKKWTTKNTGSATCKRGFVQFIYEPIKQIINICMNDQKDKLWPMLQKLGVVMKSDEKELVAKALMKRVMQTWLPASSALLEMMIFHLPSPAKAQRYRVENLYEGPLDDVYATAIRNCDPEGPLMLYVSKMIPASDKGRFFAFGRVFAGRVATGMKVRIMGPNYVPGQKKDLYTKSVQRTVIWMGKRQESVEDVPCGNTVALVGLDQFITKNATLTNEKEADAHPIRAMKFSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCTIEESGEHIIAGAGELHLEICLKDLQDDFMGGAEIVVSDPVVSFRETVLEKSCRTVMSKSPNKHNRLYFEARPLEEGLAEAIDDGRIGPKDDPKARAKILSEEFGWDKDLAKKIWCFGPETTGPNMVVDMCKGVQYLNEIKDSVVAAFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQVIPTARRVMYASQLTAKPRLLEPVYLVEIQAPENALGGIYGVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSTLRAATSGQAFPQCVFDHWDMMGSDPLEPGSQAGQLVTDIRKRKGLKESMTPLSEFEDKL*

Protein Length: 845

Fln nts: CGTCGTGACCTTGGCTGTCACTCACTGGAACAGGGTTTCGATTCGCTACCTGTTTAGTGTCCAAGTATTTCCTCAATCCACGGCATCTTATTCTGCCCCTCACAGAATATTCGATCACCGTCCTGCTCCACCAAAAACCCTAACGGAGTTACGGTCTTCAACCAAAAGCCTACTCCATCGAGCAAAGTCCATCCTTTTGTTTGTCTCAGGCCGCCGGGTCATTCTTTTATCTTAGTACTCGCATGCCTTGCCTGAGCCGCGAGAGTCCGTGATTTGAAGGTTTCAGAGGTCTGCAACTCGAATCCATTCTGTGCCGTTCTCCTTTGGCGGGTGCTCATTGGTATTCTTCTGTTTTGGCTGAGAAGAGTTTTGGTGAATACAAAAGTTAGGAAAA_-_ATGGTGAAGTTCACGGCTGAAGAGCTTCGTAAAATCATGGACAGGAAGCATAACATACGAAACATGTCTGTTATTGCCCATGTTGATCACGGAAAATCCACTCTCACTGATTCTTTGGTAGCAGCAGCTGGTATCATTGCTCAAGAAGTTGCTGGAGATGTCAGAATGACTGATACTCGGGCAGATGAAGCTGAACGTGGCATCACCATCAAGTCAACTGGAATTTCGTTGTACTATGTGATGGAGGATTCAGCGGTGAAAAATTTCAAGGGGGATAGAGAAGGACACGAATATTTGATAAATCTGATTGATTCTCCAGGGCATGTTGACTTTTCTTCAGAAGTTACAGCAGCCCTGCGAATCACAGATGGTGCACTGGTTGTGGTAGATTGTATTGAAGGGGTTTGTGTCCAGACTGAGACTGTTCTTCGTCAAGCTCTTGGAGAAAGGATTAGGCCTGTTCTCACTGTGAACAAGATGGACAGGTGCTTTCTTGAGTTGCAGGTTGATGGAGAGGAAGCTTACCAAACCTTCTCACGTGTCATTGAAAATGCAAATGTGATTATGGCTACTTATGAAGATCCTCTTCTTGGTGACTGCCAAGTTTATCCTGAAAAAGGTACAGTTGCATTCTCGGCAGGTCTGCACGGGTGGGCATTTACCCTCACAAATTTTGCAAAAATGTATGCTTCAAAGTTTGGAGTGGATGAAGCAAAGATGATGGAGAGGCTCTGGGGTGAGAACTTTTTTGATCCAGCTACAAAGAAATGGACAACCAAGAATACAGGTTCTGCAACTTGCAAAAGAGGATTTGTTCAATTCATATATGAACCTATTAAGCAAATTATCAATATTTGTATGAATGATCAAAAGGATAAGTTGTGGCCCATGTTGCAGAAATTGGGAGTTGTAATGAAAAGTGACGAGAAGGAGCTGGTTGCAAAAGCACTCATGAAGCGTGTCATGCAAACCTGGCTACCTGCAAGTAGTGCTTTACTTGAAATGATGATTTTCCATCTTCCTTCTCCAGCGAAGGCACAGAGATATCGTGTGGAAAACTTGTATGAAGGACCTCTTGATGATGTATATGCAACTGCAATAAGAAACTGTGACCCTGAGGGTCCACTGATGTTGTATGTTTCAAAAATGATTCCAGCTTCGGACAAAGGACGTTTCTTTGCCTTTGGTCGTGTTTTTGCTGGCAGAGTTGCAACAGGTATGAAGGTAAGGATCATGGGTCCCAACTATGTGCCAGGTCAGAAGAAAGATTTATATACAAAGAGTGTTCAAAGGACTGTGATATGGATGGGAAAGAGACAAGAATCTGTTGAGGATGTGCCTTGTGGAAATACAGTGGCTTTGGTTGGGTTAGACCAGTTCATAACCAAAAATGCTACACTGACAAACGAGAAGGAGGCTGATGCTCATCCGATCAGAGCAATGAAGTTCTCTGTGTCCCCTGTTGTACGTGTTGCTGTGCAGTGCAAGGTGGCATCTGACCTTCCAAAACTTGTTGAAGGGCTGAAGCGTCTTGCTAAGTCGGATCCTATGGTTGTTTGTACAATTGAGGAATCTGGAGAACATATTATTGCAGGTGCAGGAGAGCTTCATCTCGAGATTTGCTTAAAAGATCTCCAAGATGATTTTATGGGTGGAGCTGAGATTGTTGTTTCTGATCCTGTTGTCTCATTTAGAGAAACTGTTTTGGAGAAATCATGCCGTACTGTTATGAGCAAGTCTCCAAATAAACATAACAGGCTTTACTTTGAAGCAAGACCTTTAGAAGAAGGTCTTGCAGAAGCAATTGATGATGGTCGCATTGGCCCAAAGGATGATCCAAAGGCTCGCGCAAAAATCCTTTCTGAGGAATTTGGATGGGACAAAGATTTGGCTAAGAAGATCTGGTGTTTTGGACCTGAAACCACTGGTCCCAATATGGTGGTGGATATGTGTAAGGGAGTTCAATACTTGAATGAAATTAAAGATTCTGTGGTTGCTGCATTCCAATGGGCATCTAAGGAAGGTGCTCTTGCAGAAGAAAATATGCGTGGTATTTGCTTTGAGGTTTGTGACGTGGTTTTGCACACTGATGCTATCCATAGAGGAGGTGGCCAGGTTATCCCAACGGCCAGAAGGGTTATGTATGCATCTCAGCTTACTGCCAAACCACGGCTTCTTGAGCCAGTATATCTCGTTGAGATTCAGGCTCCTGAGAATGCCCTTGGTGGTATTTATGGTGTGCTGAACCAGAAGAGAGGTCATGTTTTTGAAGAAATGCAGAGGCCAGGTACTCCACTATACAACATCAAGGCATATCTGCCAGTTGTTGAATCATTCGGATTCTCAAGCACTCTCCGTGCTGCAACTTCTGGCCAGGCTTTCCCTCAATGTGTGTTTGACCACTGGGATATGATGGGCTCTGATCCATTGGAACCTGGTTCCCAAGCTGGGCAGCTTGTGACTGATATCCGTAAGAGGAAGGGTCTTAAGGAGAGTATGACTCCCTTGTCAGAGTTCGAAGACAAGCTGTAG___AGCTTTGCTATGTTTGCATGTCGGATGCTGTCAAGATTGAGGAACCTCCGAGTATTAAAACACAGTTTTGTGTGCTAGGACTAGTTGAATTTATGCTATTCAGGTATTTTTGTGATCTGTTATTTATGTTATTCAGGTATTTTTGTGATTTGAAAATACTTTTTACAAGGTACATCCATTAATCTTTTAAATGTTACATAATTCTCCCTTGTCAATTTCACCGTATGTGCGGTAGTTTGAATTGCTTGCATAATTATGTATGTCACAGAAAAGCCGAGCTGAGATTTGAGTAGACGTTGGTTTAAAAATTGTTGTATATATTTGTGCTCCATCTATTATTCTCCTCTCTAGTTTCCCAATCCATCCACGTAGTATTGTGCCTGTTGTGTCTTTTGACGTCAATGGGTTTTCCCTGTTAGTCTTATGTTATCCATCTGTGTCCAGTTCAAAGAGAAGCAAAACGAATCCACACGATAACCTGATTAA

Fln Alignment:

sp_v1.1_unigene19181___MSKSPNKHNRLYFEARPLEEGLAEAIDDGRIGPKDDPKARAKILSEEFGWDKDLAKKIWCFGPETTGPNMVVDMCKGVQYLNEIKDSVVAAFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQVIPTARRVMYASQLTAKPRLLEPVYLVEIQAPENALGGIYGVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSTLRAATSGQAFPQCVFDHWDMMGSDPLEPGSQAGQLVTDIRKRKGLKESMTPLSEFEDKL
D5ACX5_______________MSKSPNKHNRLYFEARPLEEGLAEAIDDGRIGPKDDPKARAKILSEEFGWDKDLAKKIWCFGPETTGPNLVVDMCKGVQYLNEIKDSVVAAFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQIIPTARRVMYASQLTAKPRLLEPVYLVEIQAPENALGGIYGVLNQKRGHVFEEMQRQGTPLYNIKAYLPVVESFGFSSTLRAATSGQAFPQCVFDHWDMMGSDPLETGSQAGQLVTDIRKRKGLKESMTPLSDFEDKL

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
135	66	33			0.997452
179	60		40		1.0
180		20		80	0.995493
183	60			40	1.0
187	60	40			1.0
188	60			40	1.0
189	60			40	1.0
191			40	60	1.0
193		20		40	1.0
194	60		40		1.0
197	40	60			1.0
198		40		60	1.0
200		40		60	1.0
201	40			60	1.0
212	75		25		1.0
213			75	25	1.0
215	28	71			1.0
219		71		28	1.0
220			71	28	1.0
225	71			28	1.0
226			28	71	1.0
229		28		71	1.0
231		28		71	1.0
232	28			71	1.0
262		85		14	0.993533
322		16		83	0.994512
325		83	16		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain