SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene19127

Length: 1103 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene19127

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	DUF761 multi-domain protein	-	-	1.4013e-45	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	43%
Blast2go	protein	-	-	8.65612e-27	59%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AA37

Fln msg: Unexpected stop codon in the beginning of your sequence,

Fln protein: S*PWLSLNLLLPMSSFPPCCPECARHSRRG*RLQFCMC*LTL*FLPYSPIRDFNTNSTYWAGLLRSITLRLSIRSGSRRSQFMRKNRRKDPV*RNRAGLLRSHNPSAVNQKQQQEEPIYEEESTRRCGITEFEEAEVNSVARPERIRTPDLRRLERPLATSRFNANYKKPASSKSAAEAKSLGVSRSKKKADTLEATWKAITEGRGPPLTRHLRKSDTWETVHRGDATEAASRDSSSSSSSAVFRKSDTWSNGRNRPENLSGKPELGAGLRKDPSLNQDELNKRVEAFIARMNNEMKLQREESLARYMEMVNRGASE*

Protein Length: 319

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTGTTGCTTCCTATGAGTTCTTTTCCGCCGTGTTGCCCCGAGTGCGCGAGACATTCTCGGCGTGGCTGACGCCTACAGTTCTGTATGTGTTAGTTAACTTTATAATTCTTGCCATACTCGCCAATTCGAGATTTCAACACAAATTCGACATATTGGGCAGGTCTCTTGAGGAGCATAACCCTTCGGCTGTCCATCAGAAGCGGCAGCAGGAGGAGCCAATTTATGAGGAAGAATCGACGGAAAGATCCGGTATAACGGAATCGGGCAGGTCTCTTGAGGAGTCATAACCCTTCGGCTGTCAATCAGAAGCAGCAGCAGGAGGAGCCAATTTATGAGGAAGAATCGACGAGAAGATGCGGTATAACGGAATTCGAAGAGGCGGAGGTGAACTCTGTAGCGAGACCTGAAAGGATTCGAACCCCGGACCTCCGCAGATTGGAAAGGCCTTTGGCTACCTCGCGCTTTAACGCCAATTACAAGAAACCCGCAAGTTCGAAGTCGGCCGCGGAGGCCAAATCGCTGGGGGTTTCGCGGTCCAAGAAAAAGGCGGACACACTGGAGGCGACGTGGAAGGCCATCACGGAAGGCCGAGGCCCTCCCCTGACGCGGCACCTTCGGAAATCTGATACCTGGGAGACGGTCCACCGTGGGGACGCTACAGAGGCCGCATCCCGCGATTCATCATCATCTTCTTCTTCTGCAGTTTTCCGAAAATCCGACACATGGAGCAACGGGAGGAATCGACCCGAGAACCTCTCCGGAAAACCTGAATTAGGTGCGGGGTTGAGAAAAGATCCCTCTCTGAACCAAGACGAGCTGAATAAACGAGTGGAGGCCTTCATTGCCAGGATGAACAATGAGATGAAGCTTCAGAGAGAAGAGTCGCTGGCTCGTTACATGGAAATGGTGAATCGAGGAGCCAGCGAGTAA___TTTTTTTTCCGTTACTGTCTGTCACTGGATTGTAATTACTTACTTAGTTACTGAGTCTTTAATTGCAGTATTGAATGTGCGGGTTGTTTAATTATTTTCTTGATAGATAGTTGATTTGCAGCTGCATTTCCTTCCAGGCATGACCAG

Fln Alignment:

sp_v1.1_unigene19127___ERPLATSRFNANYXXXXXXXXXXXXXXLGVSRSKKKADTLEATWKAITEGRGPPLTRHLRKSDTWETVHRGDATEAASRDXXXXXXXAVFRKSDTWSNGRNRPENLSGKPELGAGLRKDPSLNQDELNKRVEAFIARMNNEMKLQREESLARYMEMVNRG
D5AA37_______________EKPLVSSRFTHK---RYAKASPDAKSPLRIARPKK-GETLEYTWKTLTERRHPPLTRHLRKSETW------DSSSPSRANADHSSPARNLRKSETMKH-RGREDSPASPC-----LKREPSLSQDELNRRVEAFISKFNMEMRLQRQNSFTSYMEMINRG

SNPs (tot: 63)

Position	A %	C %	G %	T %	Probability
12		16		83	0.994512
15	16	83			0.994512
16	16			83	0.994512
23		83	16		0.994512
24	83	16			0.994512
37		85	14		0.993533
39	85	14			0.993533
43	28	14	57		1.0
46		85	14		0.993533
47			14	85	0.993533
48		14		85	0.993533
50			14	85	0.993533
51		85		14	0.993533
53	14		85		0.993533
56	11		88		0.923564
58		11	88		0.923564
59			11	88	0.923564
61			66	33	1.0
62	11	88			0.923564
65		88		11	0.923564
66			88	11	0.923564
67	88		11		0.923564
71		88		11	0.923564
76		11	88		0.923564
77	88			11	0.923564
88			55	44	1.0
136		36		63	1.0
180		40		60	1.0
228	40		60		1.0
402			66	33	0.997452
422	83	16			0.994512
426	83			16	0.994512
433	83		16		0.994512
434	83	16			0.994512
435	83			16	0.994512
437			83	16	0.994512
439		16		83	0.994512
442	16		83		0.994512
473			25	75	1.0
475		25		75	1.0
477			75	25	1.0
478		75	25		1.0
484		75		25	1.0
485	12		87		0.992554
490			25	75	1.0
492	75	25			1.0
495			75	25	1.0
748		90		10	0.90298
749	90		10		0.90298
750			10	90	0.90298
879	90			10	0.90298
880	90			10	0.90298
881		90	10		0.90298
882	90		10		0.90298
883	90	10			0.90298
886	90			10	0.90298
887		10	90		0.90298
889		10		90	0.90298
890			90	10	0.90298
891	90	10			0.90298
902	60		40		1.0
953			50	50	1.0
958	20			60	0.99255

SSRs (tot: 1)

Start position	End position	Sequence	Length
714	725	TCT TCT TCT TCT	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain