SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene18926

Length: 1536 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene18926

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	AMI3; amidase family protein	-	-	53%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	90%
Blast2go	amidase family protein	-	-	75%

Source	ECs	Term	Type	e value	Identity
Blast2go	Ligases, Forming carbon-nitrogen bonds, Carbon--nitrogen ligases with glutamine as amido-N-donor.	EC:6.3.5.-	-	0.0	75%

Source	KEGGs	Term	Type	Identity
Blast2go	Tetracycline biosynthesis	00253		75%
Blast2go	Biosynthesis of secondary metabolites	01110		75%
Blast2go	Amidase.	EC:3.5.1.4	-	75%

Source	KEGGs	Term	Identity
Blast2go	Arginine and proline metabolism	00330	75%
Blast2go	Phenylalanine metabolism	00360	75%
Blast2go	Tryptophan metabolism	00380	75%
Blast2go	Cyanoamino acid metabolism	00460	75%
Blast2go	Aminobenzoate degradation	00627	75%
Blast2go	Styrene degradation	00643	75%
Blast2go	Biosynthesis of plant hormones	01070	75%

Source	GOs	Term	Type	Identity
Blast2go	indoleacetic acid biosynthetic process	GO:0009684	Biological Process	75%
Blast2go	carbon-nitrogen ligase activity, with glutamine as amido-N-donor	GO:0016884	Molecular Function	75%
Blast2go	protein binding	GO:0005515	Molecular Function	75%
Blast2go	indoleacetamide hydrolase activity	GO:0043864	Molecular Function	75%
Blast2go	amidase activity	GO:0004040	Molecular Function	75%
Blast2go	cytoplasm	GO:0005737	Cellular Component	75%

Source	InterPros	Term	Type	Identity
Blast2go	Amidase	IPR000120	-	75%
Blast2go	Amidase, conserved site	IPR020556	-	75%
Blast2go	Amidase signature domain	IPR023631	-	75%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PSB6

Fln msg: Distance to subject end: 27 aas, your sequence is shorter than subject: 430 - 458

Fln protein: MSAANLWVLLGLGLAGIFLASRRRKKGIKEDFGAFIERIKLLPPPQPSPPKAPHPLTGLTFAIKELFDIEGYVTGFGNPDWRRTHEPAVRTAPVVTFVVQGGATCVGRTVMDEMAYSISGENKHYGTPTNPAAPSRIPGGSSSGSAVAVAAELVDFALGTDTGGSVRVPAAFCGILGFRPSHGAVSTIGVVPMAQSFDTVGLFTRDPNILRHVGHILLQLPFMEYRQPRRIIIADDCFQVTDIPNDRTVNVVTRSTEKLFGRQVLNHMSLGNYLATKVPSLKYFQNEEESRNGEGGVSALKALCSALRLLQRYEFKMNHEEWINSVKPDFGPGISTRVRAALETNNENIGHCLKARDEAREAINGLLKDDAILIIPTTPGPAPKLNMKENLLEDFRGRAFTLLSISGMSGCCQVSIPVGQHDKCPLAVSM

Protein Length: 431

Fln nts: ATGGGGAGGAGGATTGGATTTTTGCCATATGTGCGTCTATAAGCATCCACTCCATTTGGCACTGGTTTTATCCAAACCCTGAGAGGGTTCGGCACAAATTTAGAAAGCTTTGATAAACACTCATAGGCAGATGAGCCAGTGGTTCCAATACTTCTATGAATTAATGCAAGAGTTTTGCCTGTAATTTATTCTTTATTTTTTAAGGGTTCCTGGATTTGGATTCGGGGGTGATTTATCGATAACA_-_ATGTCTGCTGCAAACCTCTGGGTGCTGCTGGGATTGGGGTTAGCGGGAATATTTCTAGCTAGCAGGAGACGGAAGAAGGGAATCAAGGAAGATTTTGGCGCATTTATTGAGCGGATTAAGCTCCTTCCGCCTCCTCAGCCCTCACCTCCTAAAGCACCGCACCCTCTTACGGGCCTTACTTTCGCAATTAAAGAACTATTTGATATAGAAGGATATGTAACTGGCTTTGGAAATCCAGACTGGCGAAGGACCCATGAACCTGCAGTTCGAACGGCACCTGTGGTTACATTTGTTGTACAAGGAGGAGCAACATGTGTTGGGAGAACTGTCATGGATGAGATGGCATACAGCATTAGTGGAGAAAACAAACACTATGGAACTCCAACTAATCCAGCTGCACCTTCACGAATACCTGGAGGATCTTCTAGTGGGTCTGCTGTGGCGGTTGCAGCTGAGCTTGTTGACTTTGCTCTTGGTACAGATACAGGAGGAAGTGTCAGAGTGCCTGCTGCATTCTGTGGTATTCTAGGGTTCCGTCCTTCCCATGGGGCTGTTTCCACCATTGGTGTTGTGCCAATGGCTCAGAGTTTTGATACTGTTGGTTTGTTTACAAGGGATCCAAATATTTTGCGTCATGTTGGTCATATACTACTACAGTTGCCATTCATGGAGTACAGGCAACCAAGGCGTATTATAATTGCAGATGATTGCTTTCAGGTTACAGATATTCCTAATGATCGAACAGTGAATGTAGTCACTCGGTCCACTGAAAAATTATTTGGACGTCAAGTTTTAAATCACATGAGCCTCGGCAATTATCTTGCTACCAAAGTTCCAAGCTTAAAGTATTTTCAAAATGAAGAAGAGAGCAGAAATGGTGAAGGTGGGGTTTCTGCTTTGAAGGCTCTTTGCAGTGCATTACGCCTTCTTCAGAGGTATGAGTTCAAAATGAATCATGAAGAGTGGATCAACAGTGTAAAGCCTGATTTTGGACCTGGTATATCTACTCGTGTACGTGCAGCTCTAGAGACAAATAATGAAAATATTGGACATTGTTTGAAGGCCAGGGATGAGGCACGTGAAGCAATTAATGGGCTTTTGAAGGACGATGCAATCCTAATCATTCCCACAACTCCAGGTCCTGCACCTAAGCTCAATATGAAAGAAAATTTGTTGGAGGACTTTCGTGGGAGGGCTTTCACACTTTTGAGTATATCTGGAATGTCTGGATGCTGTCAGGTGAGCATACCTGTAGGGCAACATGATAAGTGTCCTTTGGCTGTGTCAATGAT

Fln Alignment:

sp_v1.1_unigene18926___MSAANLWVXXXXXXXXIFLASRRRKKGIKEDFGAFIERIKXXXXXXXXXXXXXXXXTGLTFAIKELFDIEGYVTGFGNPDWRRTHEPAVRTAPVVTFVVQGGATCVGRTVMDEMAYSISGENKHYGTPTNXXXXXXXXXXXXXXXXXXXXXELVDFALGTDTGGSVRVPAAFCGILGFRPSHGAVSTIGVVPMAQSFDTVGLFTRDPNILRHVGHILLQLPFMEYRQPRRIIIADDCFQVTDIPNDRTVNVVTRSTEKLFGRQVLNHMSLGNYLATKVPSLKYFQNEEESRNGEGGVSALKALCSALRLLQRYEFKMNHEEWINSVKPDFGPGISTRVRAALETNNENIGHCLKARDEAREAINGLLKDDAILIIPTTPGPAPKLNMKENLLEDFRGRAFTLLSISGMSGCCQVSIPVGQHDKCPLAVSM
C0PSB6_______________MSATKICVLLGLGLAGIFLASRRRKKEIKEDFGAFTERIQLLPPPQPSPPESPYPLTGLTFAIKEIFDIEGYVTGFGNPDWQQTHEPAAQTAPVVTFVVQGGATCVGRTVMDEMAYSINGENKHYGTPTNPAAPSRIPGGSSSGSAVAVAAELVDFALGTDTGGSVRVPASFCGILGFRPSHGAVSTVGVVPMAQSFDTVGLFTRDPNILRHVGHILLQLPFMEYRQPRGIIIADDCFQLTKIPNDQTVNVVTRSTEKLFGRQVLNHISLGEYIATEVPSLKYFQNEEESRDGECGISALKALCSALRLLQRYEFKMNHEEWINSVKPDLGPGISGRVRAALETNNENIEHCLKAKDEAREAVNSLLKDDAILIIPTTPGPAPKLNMKEMLLEEFRIRAFTLLCISGMSGCCQVSIPVGQHDKCPLAVSM

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
66	40	60			1.0
72			40	60	1.0
73			40	60	1.0
131			66	33	1.0
346			83	16	0.994512
395		14		71	0.984337
492	14	85			0.993533
493		14	85		0.993533
508		88		11	0.923564
565		12		87	0.992554
566	12			87	0.992554
568		12	87		0.992554
569			87	12	0.992554
677		12		87	0.992554
798	16		83		0.994512
807	83		16		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain