SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene18266

Length: 1945 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene18266
TTCAACTGCAGTGAGCGACATTGCATCAGATGGGGGAGCCCAGAGGCGCGTGAGGTTGGCGTCATTCCATTCCCGTACATTTTACCCATCTGCAAAATGCAAATACCATATTTCTAGGACCATTCCAGGCTACAAATCCTCGTGAATGCAAGTGAATTCCTCTCTCTCATCACAATGATCTCTGTTCGAATCCTTTCGGATTCCATTCTCTGGAGTAGAAAACTGAAGACGCGTCTGTTCATTCTTCAGGAATTTCGGTTGCTGCTGCTGAGCTACATTATTGCAATAGGTTTCGGATTGGAGATACAGCAGCGTACAACATCCTGCAGCCTGAACAGAGCATGGTTATCCAGGCCTCACAGTGTGAGTATAACGGAGTTTGGAGCAGTGGGTGATGGGGTTACTCTGAACACACATGCTTTTCAGAATGCCATTTTCTACGTTAACACCTTTGCTGACAAAGGTGGGGCTCAGCTCTATGTTCCGGCAGGAAAATGGTTGACTGGTAGCTTTAATCTCACTAGTCATCTTACACTGTACTTGGATAGAGACTCTGTCATTCTCGGATCCCAGGAGTCGCGAGATTGGCCAGTTATTGATCCCTTACCATCTTATGGCCGAGGAAGAGAGCTACCTGGTGGAAGACACTGCAGCCTCCTACACGGTTACAACCTTACAGATGTTGTCATAACAGGGGATAATGGAACTGTTGACGGCCAGGGTGCCATTTGGTGGGATTGGTTTCATAACCATACGCTTAATTTCACTCGGCCTCACCTTGTGGAGCTGAGAAGCTCGACTGACATTGTGATTGCAAATTTGACATTTCTGAATTCACCATTTTGGAACATTCATCCAGTGTATAGCAGCAATGTGCGAATCCGGAATGTTACGATTCTTGCACCATCCGACTCACCTAATACGGATGGAATCGATCCAGATTCCTGCAATGATGTCTGCATAGAAGATTGTTACATCAGCACCGGTGATGATTCAATTGCGATAAAGAGTGGCTGGGATGAGTATGGTATTGCTTTTGGCCGTCCTAGTTTCAACATTATTATCCGCCACATAGTCAGTCAAAGCCATACAAGTGCAGGACTTGCATTGGGTAGTGAAATGTCTGGAGGGATCACAGATATTCATGTAGAAGACATTCTTGTAATCAACACAGAAACAGCTATCAGTGTAAAGACGTCTGCAGGCAGAGGAGGTTATGTGAAGGGCATCTTTATATCCAATGTGACCATGAACAATGTGAAGCGTGCCATTGGTTTTACTGGGTTCTATGGTGATCATCCTGATGATCGCTATAATCCTGATGCTCTCCCTGTTGTAGATAGGATCATCTTTAAGGACATTGTGGGTGAGAATATCACTATTGCCGGTGACTTTCATGGGATCGAGAAAGCTCCCTTCAGTAATATTTGTATGTCAAACATTTTCTTCAATGTCACCTCAGAAGCTGCATGGAGCTGTTCAAATGTGAAAGGCTTTTCAGAGTCAGTTTATCCTCAGCCTTGTGCAGATCTTCAGCATCGTATCTCCAATGAGTCCTCAGTTTGTTCATTTTCATCTGTGAGCAATTCATTCAAAGACCTCTAGGATGAAGCCAATTGTTCAAATTGCCTGTTATTGAGTTCAACCTTAGCTATTGCTAGGTTAATGCAAGCTCCCATTTTTCACGCATTTTTTTGGAGATCACAGTATCACAATCCTTGAGATAGTGGTTCATGTGCAATTCTCGGTAATGTCAAATGCCTACCTTACTATTCATCACTTTCCACATTTCTTTTTATCTCTGCAGTATTCACCCTCTATGGCCTGATCCCAATTTTGCGAGATGGCTGGTCCAAAATTTCCACATTCTTTATCCCTGCAGTATTCACCCTCTATGGCCTGATCCAAATTTTGCGAGATGGCTGGTCCAAACTTTCCACATTTC

Ace file of the UniGene sp_v1.1_unigene18266

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Polygalacturonase precursor, putative (EC:3.2.1.67)	-	-	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	59%
Blast2go	glycoside hydrolase family 28 expressed	-	-	79%

Source	ECs	Term	Type	e value	Identity
Blast2go	Galacturan 1,4-alpha-galacturonidase.	EC:3.2.1.67	-	0.0	79%

Source	KEGGs	Term	Type	Identity
Blast2go	Pentose and glucuronate interconversions	00040		79%
Blast2go	Starch and sucrose metabolism	00500		79%
Blast2go	Metabolic pathways	01100		79%
Blast2go	Polygalacturonase.	EC:3.2.1.15	-	79%

Source	KEGGs	Term	Identity
Blast2go	Pentose and glucuronate interconversions	00040	79%
Blast2go	Starch and sucrose metabolism	00500	79%
Blast2go	Metabolic pathways	01100	79%

Source	GOs	Term	Type	Identity
Blast2go	carbohydrate metabolic process	GO:0005975	Biological Process	79%
Blast2go	galacturan 1,4-alpha-galacturonidase activity	GO:0047911	Molecular Function	79%
Blast2go	polygalacturonase activity	GO:0004650	Molecular Function	79%
Blast2go	vacuole	GO:0005773	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Blast2go	Glycoside hydrolase, family 28	IPR000743	-	79%
Blast2go	Parallel beta-helix repeat	IPR006626	-	79%
Blast2go	Pectin lyase fold/virulence factor	IPR011050	-	79%
Blast2go	Pectin lyase fold	IPR012334	-	79%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQW0

Fln msg:

Fln protein: MISVRILSDSILWSRKLKTRLFILQEFRLLLLSYIIAIGFGLEIQQRTTSCSLNRAWLSRPHSVSITEFGAVGDGVTLNTHAFQNAIFYVNTFADKGGAQLYVPAGKWLTGSFNLTSHLTLYLDRDSVILGSQESRDWPVIDPLPSYGRGRELPGGRHCSLLHGYNLTDVVITGDNGTVDGQGAIWWDWFHNHTLNFTRPHLVELRSSTDIVIANLTFLNSPFWNIHPVYSSNVRIRNVTILAPSDSPNTDGIDPDSCNDVCIEDCYISTGDDSIAIKSGWDEYGIAFGRPSFNIIIRHIVSQSHTSAGLALGSEMSGGITDIHVEDILVINTETAISVKTSAGRGGYVKGIFISNVTMNNVKRAIGFTGFYGDHPDDRYNPDALPVVDRIIFKDIVGENITIAGDFHGIEKAPFSNICMSNIFFNVTSEAAWSCSNVKGFSESVYPQPCADLQHRISNESSVCSFSSVSNSFKDL*

Protein Length: 478

Fln nts: TTCAACTGCAGTGAGCGACATTGCATCAGATGGGGGAGCCCAGAGGCGCGTGAGGTTGGCGTCATTCCATTCCCGTACATTTTACCCATCTGCAAAATGCAAATACCATATTTCTAGGACCATTCCAGGCTACAAATCCTCGTGAATGCAAGTGAATTCCTCTCTCTCATCACA_-_ATGATCTCTGTTCGAATCCTTTCGGATTCCATTCTCTGGAGTAGAAAACTGAAGACGCGTCTGTTCATTCTTCAGGAATTTCGGTTGCTGCTGCTGAGCTACATTATTGCAATAGGTTTCGGATTGGAGATACAGCAGCGTACAACATCCTGCAGCCTGAACAGAGCATGGTTATCCAGGCCTCACAGTGTGAGTATAACGGAGTTTGGAGCAGTGGGTGATGGGGTTACTCTGAACACACATGCTTTTCAGAATGCCATTTTCTACGTTAACACCTTTGCTGACAAAGGTGGGGCTCAGCTCTATGTTCCGGCAGGAAAATGGTTGACTGGTAGCTTTAATCTCACTAGTCATCTTACACTGTACTTGGATAGAGACTCTGTCATTCTCGGATCCCAGGAGTCGCGAGATTGGCCAGTTATTGATCCCTTACCATCTTATGGCCGAGGAAGAGAGCTACCTGGTGGAAGACACTGCAGCCTCCTACACGGTTACAACCTTACAGATGTTGTCATAACAGGGGATAATGGAACTGTTGACGGCCAGGGTGCCATTTGGTGGGATTGGTTTCATAACCATACGCTTAATTTCACTCGGCCTCACCTTGTGGAGCTGAGAAGCTCGACTGACATTGTGATTGCAAATTTGACATTTCTGAATTCACCATTTTGGAACATTCATCCAGTGTATAGCAGCAATGTGCGAATCCGGAATGTTACGATTCTTGCACCATCCGACTCACCTAATACGGATGGAATCGATCCAGATTCCTGCAATGATGTCTGCATAGAAGATTGTTACATCAGCACCGGTGATGATTCAATTGCGATAAAGAGTGGCTGGGATGAGTATGGTATTGCTTTTGGCCGTCCTAGTTTCAACATTATTATCCGCCACATAGTCAGTCAAAGCCATACAAGTGCAGGACTTGCATTGGGTAGTGAAATGTCTGGAGGGATCACAGATATTCATGTAGAAGACATTCTTGTAATCAACACAGAAACAGCTATCAGTGTAAAGACGTCTGCAGGCAGAGGAGGTTATGTGAAGGGCATCTTTATATCCAATGTGACCATGAACAATGTGAAGCGTGCCATTGGTTTTACTGGGTTCTATGGTGATCATCCTGATGATCGCTATAATCCTGATGCTCTCCCTGTTGTAGATAGGATCATCTTTAAGGACATTGTGGGTGAGAATATCACTATTGCCGGTGACTTTCATGGGATCGAGAAAGCTCCCTTCAGTAATATTTGTATGTCAAACATTTTCTTCAATGTCACCTCAGAAGCTGCATGGAGCTGTTCAAATGTGAAAGGCTTTTCAGAGTCAGTTTATCCTCAGCCTTGTGCAGATCTTCAGCATCGTATCTCCAATGAGTCCTCAGTTTGTTCATTTTCATCTGTGAGCAATTCATTCAAAGACCTCTAG___GATGAAGCCAATTGTTCAAATTGCCTGTTATTGAGTTCAACCTTAGCTATTGCTAGGTTAATGCAAGCTCCCATTTTTCACGCATTTTTTTGGAGATCACAGTATCACAATCCTTGAGATAGTGGTTCATGTGCAATTCTCGGTAATGTCAAATGCCTACCTTACTATTCATCACTTTCCACATTTCTTTTTATCTCTGCAGTATTCACCCTCTATGGCCTGATCCCAATTTTGCGAGATGGCTGGTCCAAAATTTCCACATTCTTTATCCCTGCAGTATTCACCCTCTATGGCCTGATCCAAATTTTGCGAGATGGCTGGTCCAAACTTTCCACATTTC

Fln Alignment:

sp_v1.1_unigene18266___IIAIGFGLEIQQRTTSCSLNRAWLSRPHSVSITEFGAVGDGVTLNTHAFQNAIFYVNTFADKGGAQLYVPAGKWLTGSFNLTSHLTLYLDRDSVILGSQESRDWPVIDPLPSYGRGRELPGGRHCSLLHGYNLTDVVITGDNGTVDGQGAIWWDWFHNHTLNFTRPHLVELRSSTDIVIANLTFLNSPFWNIHPVYSSNVRIRNVTILAPSDSPNTDGIDPDSCNDVCIEDCYISTGDDSIAIKSGWDEYGIAFGRPSFNIIIRHIVSQSHTSAGLALGSEMSGGITDIHVEDILVINTETAISVKTSAGRGGYVKGIFISNVTMNNVKRAIGFTGFYGDHPDDRYNPDALPVVDRIIFKDIVGENITIAGDFHGIEKAPFSNICMSNIFFNVTSEA---AWSCSNVKGFSESVYPQPCADLQHR
B8LQW0_______________LIVLGVKVECQKGSWSFSYV-APSCRAQSVSIAEFGGVGDGTTVNTEAFRKAIEHLSEFSETGGGQLYIPPGRWLTGSFNLTDHFTLYLHKEAVILGSQDLEDWPLIPPLPSYGMGRDAPGPRYSSLINGYNLTDVVITGDNGTIDGQGAIWWQKFHKKILKNTRGYLVELMYSKDIIISNLTFLNSPAWNLHPVYSSNILIQYVTILAPLDSPNTDGIDPDSCSYVRIEDCYVVSGDDIVAIKSGWDEYGISFGMPSQHIVIRRLVGISPTSAIIALGSEMSGGIQDVRAEDIQAINSETGIRIKTSPGRGGFVKDIFVNRMTMVNMKWAFTMTGSYGSHPDNKYDPNALPAVERISYSNIVATNVSVAGKLDGIANAPFKDICLTNVTITMAAKSKKYPWNCTYIHGLSNAVYPQPCSLLEER

SNPs (tot: 67)

Position	A %	C %	G %	T %	Probability
132	75	25			0.996473
134			75	25	0.996473
141	50	25			0.995533
142	75			25	0.996473
143	75			25	0.996473
144			25	75	0.996473
145		75	25		0.996473
151		25	75		0.996473
153	75	25			0.996473
155		25	75		0.996473
156	25	75			0.996473
320		75		25	0.996473
421	85		14		0.993533
423	85		14		0.993533
429			14	85	0.993533
430			14	85	0.993533
432	85			14	0.993533
434	85			14	0.993533
435	85	14			0.993533
436	14			85	0.993533
437		14	85		0.993533
438		85		14	0.993533
669	83		16		0.994512
832	75		25		1.0
876			87	12	0.992554
878	87		12		0.992554
879	87	12			0.992554
880	12			87	0.992554
884	12	87			0.992554
981	88		11		0.923564
983	90		10		0.90298
986	90		10		0.90298
987			90	10	0.90298
988		90	10		0.90298
998		10	90		0.90298
1000		10		90	0.90298
1215	88		11		0.923564
1216		25	62	12	1.0
1217	62	12		25	1.0
1218		25	75		1.0
1219	12		75		0.975346
1221		12	87		0.992554
1222		12	87		0.992554
1225	87		12		0.992554
1226			12	87	0.992554
1227		18	72		0.999994
1229		11	88		0.923564
1230	80			20	0.999996
1231	60	20		10	0.999999
1232	20		80		0.999996
1233		30	70		1.0
1234		10	70	20	0.999998
1236	90			10	0.90298
1239	10			90	0.90298
1240		10		90	0.90298
1245	10			90	0.90298
1348	90		10		0.90298
1349		10		90	0.90298
1350	90			10	0.90298
1352			90	10	0.90298
1478	84		15		0.999986
1479		15		76	0.999983
1480		7	76	15	0.999983
1481		15	84		0.999986
1484	7	76			0.999983
1809		16		83	1.0
1840	18	81			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain