SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene17810

Length: 1613 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene17810

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cinnamoyl-CoA reductase n=1 Tax=Camellia oleifera RepID=C3VPA5_9ERIC	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	91%
Blast2go	cinnamoyl- reductase	-	-	82%

Source	ECs	Term	Type	e value	Identity
Blast2go	Cinnamoyl-CoA reductase.	EC:1.2.1.44	-	0.0	82%

Source	KEGGs	Term	Type	Identity
Blast2go	Phenylpropanoid biosynthesis	00940		82%
Blast2go	Biosynthesis of phenylpropanoids	01061		82%
Blast2go	Metabolic pathways	01100		82%
Blast2go	Biosynthesis of secondary metabolites	01110		82%
Blast2go	Dihydrokaempferol 4-reductase.	EC:1.1.1.219	-	82%

Source	KEGGs	Term	Identity
Blast2go	Flavonoid biosynthesis	00941	82%
Blast2go	Biosynthesis of phenylpropanoids	01061	82%
Blast2go	Metabolic pathways	01100	82%
Blast2go	Biosynthesis of secondary metabolites	01110	82%

Source	GOs	Term	Type	Identity
Blast2go	lignin biosynthetic process	GO:0009809	Biological Process	82%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	82%
Blast2go	cinnamoyl-CoA reductase activity	GO:0016621	Molecular Function	82%
Blast2go	coenzyme binding	GO:0050662	Molecular Function	82%
Blast2go	dihydrokaempferol 4-reductase activity	GO:0045552	Molecular Function	82%

Source	InterPros	Term	Type	e value	Identity
Blast2go	NAD-dependent epimerase/dehydratase	IPR001509	-	0.0	82%
Blast2go	NAD(P)-binding domain	IPR016040	-	0.0	82%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NU33

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 37 aas, putative chimeric sequence, your sequence is shorter than subject: 286 - 322

Fln protein: MGSKETVCVTGASGFIGSWVVRLLLERGYSVHGTVQDLGNEKETKHLEAMEGAKERLKLFQIDLLDYGSIETAINGSVGVFHLASPCIVEEVKNPQAQLLDPAVKGTMHVLQAAQKAKVKRLVLTSSISAIIPSPNWPADLPKDENCWTDLDYCKENGIWYPASKTLAEKAAWDFAKETGLDVVVINPGTVMGPILPPAINASMAMFLQLLQGGTEAYQNFYMGCVHVKDVAEGHILLYETPSASGRHLCVEATNHWSDFAEMVAKLYPEYKIHRTSMYIQRNDQ*

Protein Length: 287

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATGTCGAAATGTTTATCTGGAACTTTGAGGCGAAATTATGTCCACCTTAGGCTAGTCAAATTTAAATATCTAAGATGACTCACACTATCAGCACTTGCAGTGATATTTGTATGTATGGTGATGATACTATCCACTTAATCTTACAGTGGAACCCGCATCGTTTTCTTAGTTTCTGGAACAAAATAACATCCTAAAATTTCCAGAAATTTGGCCACATCGCAGAGGACCAGCACCAGCATTATAGTCACAGTGGAGAGCAATTAAATACACTATCGGGATTTTAACATAAGGTTTTAAGCATTTATTATCATTTCAAGTCTGCAGGCAGGGAATTACCAGTGTGAAA_-_ATGGGTAGCAAAGAAACTGTATGTGTAACCGGAGCTAGTGGATTCATCGGGTCATGGGTTGTTCGTTTGCTGCTAGAACGAGGCTACTCTGTGCACGGAACTGTTCAAGATCTCGGAAATGAGAAGGAGACTAAGCACCTAGAGGCCATGGAAGGAGCCAAGGAGAGACTGAAATTGTTTCAAATAGACCTGCTGGATTATGGTTCTATCGAGACTGCCATTAATGGGTCTGTTGGTGTTTTCCATTTAGCGTCTCCATGTATTGTCGAAGAAGTCAAGAACCCACAAGCCCAGCTTTTGGATCCTGCAGTTAAGGGTACCATGCATGTCTTGCAAGCAGCGCAGAAAGCCAAAGTGAAACGTCTGGTGTTAACCTCCTCAATCTCTGCAATTATTCCCAGTCCGAACTGGCCTGCAGATTTACCGAAGGATGAGAACTGTTGGACTGATCTTGATTACTGCAAAGAAAATGGGATTTGGTATCCTGCATCAAAAACACTCGCTGAAAAAGCAGCTTGGGATTTTGCCAAAGAGACTGGTTTGGATGTTGTTGTTATCAATCCAGGAACTGTGATGGGACCGATTCTGCCTCCGGCCATTAATGCTAGTATGGCCATGTTTCTTCAGCTTCTTCAAGGTGGCACAGAAGCATATCAGAATTTTTACATGGGCTGTGTACATGTAAAAGATGTTGCGGAAGGACATATATTACTATATGAAACCCCATCAGCATCAGGAAGGCACCTTTGTGTTGAAGCAACAAACCATTGGAGTGACTTTGCTGAGATGGTGGCAAAACTATACCCTGAATATAAAATACACAGGactAGCATGTACATACAAAGGAATGATCAATAA___CgaTcGCTtACCTgactTgTaActCATGTTTGATCGGGGTTTGCGGTGGCAGTGACTGGTTTCTGTGATGGGTAACAATGTTTGAATTGGTAGATTGATGAGTGGCTATGCATTGGTgTGATTTGTTCTGAATTGATCAAGTTGCATTGGTTACATTAGAAGATTTGATGATCCAGTATTTGAGTAGTTGATTTCAGAATTGTTGAGTTTGGATCTAGTTTGAAACATTTTGAACAGTGATCCACAAAGAGCCGAGGATAATAGTCTTAATAAAAGGGGGTTGTGGCTCCCTTCCAGTAGGAGTTGGAAAACGAGAGAAGTTTGTAGTTTTCTGCAGGTAGGAGCCTGCGTACTGGCTAAAGTTTGCGTCAGTACAAGATTGTAA

Fln Alignment:

sp_v1.1_unigene17810___MGSKETVCVTGASGFIGSWVVRLLLERGYSVHGTVQDLGNEKETKHLEAMEGAKERLKLFQIDLLDYGSIETAINGSVGVFHLASPCIVEEVKNPQAQLLDPAVKGTMHVLQAAQKAKVKRLVLTSSISAIIPSPNWPADLPKDENCWTDLDYCKENGIWYPASKTLAEKAAWDFAKETGLDVVVINPGTVMGPILPPAINASMAMFLQLLQGGTEAYQNFYMGCVHVKDVAEGHILLYETPSASGRHLCVEATNHWSDFAEMVAKLYPEYKIHR
A9NU33_______________MGSKETVCVTGASGFIGSWIVRLLLERGYSVQGTVQDLGNEKETKHLEAMEGAKERLKLFQMDLLDYGSIEAAINGSVGVFHLASPNIIEEVENPQAQLLDPAVKGTKHVLQAAQKAKVKRLVLTSSTSAIIPSPNWPANVPKDENCWTDLDYCKENGIWYPVSKTLAEKTAGDFAKETGLDVVVINPGPVMGPILPPAINASMTMFRQLLQGGTEGYQNIYMGCVHVKDVAEGHILLYETSSASGRHLCIEAITHWSDFAEMVAKLYPEYKIHR

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
314		20	80		0.995493
317		20		80	0.995493
318			20	80	0.995493
320	80		20		0.995493
322		20	80		0.995493
327			20	80	0.995493
328	80		20		0.995493
329		20		80	0.995493
331	80		20		0.995493
335		20		80	0.995493
340	80		20		0.995493
641	33	66			0.997452
773		16		83	0.994512
1231	50	50			1.0
1232	50		50		1.0
1234	50	50			1.0
1238			50	50	1.0
1243	50			50	1.0
1244		50	50		1.0
1245	50		50		1.0
1246	50	50			1.0
1248			50	50	1.0
1250		50	50		1.0
1252		50		50	1.0
1253			50	50	1.0
1347	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain