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Fln status: Internal

Fln database: tr_plants

Fln subject: B9S8J3

Fln msg: Overlapping hits, possible frame ERROR between 280 and 231, and possible frame ERROR between 875 and 877, Unexpected stop codon in the beginning of your sequence, Distance to subject end: 464 aas, your sequence is shorter than subject: 613 - 1594

Test code: 1

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: WETGLSS*PWLSLKYAILDDDKSGLTLHFMEVTVKDPTVGGTSEGRSNGELDPNSFSESSSVNDRAPLQFIFETEVxxxxxxxxxxxxxxxxxxGSHIGLAKLFASTYRISSDHGQYLSTKLEPGKSIKLKSNETVLQVQWQETRTGQVAGILTTHRVMIASANIEILSCSSTSFDKGFPPFRSLLWVGPALLFSTATAVGILGWDGLVRTVVTINTPYAALVGTLNDRLLLACTMDANPRQKQSVEIKTRLVGLLEPLLIGWATMQQKFESKLNLSEILYQLTSRFDSLRxFTPRSVDVLANGPPVCGDLAVALAQAGPQFTQVLRCNYAIKSHRFSMALSVLKDEFLRSRDYPQCPPTSRLFQRFRQLGQACIKYGQFDSAKETFEVISDFESMLDLFICHLNPSALRRLAQKLEDMDAESELRRQCERILRVRSSGWTQGIFANFAAESMVPKGPEWGGGNWEIKTPPESKNIPQWELSGEVMPYMKTSAGFIPSIIPDHIGVYLGTMKGRGTIIDVRDDTSLKRFTQSVNGESKTEELPLALINSESSNLKRSDDGIVKGPPATASQSLSKAFQGDAAADKQARAAEEFKKPLYGGDDSSSEEDDTSSN

Protein Length: 614

Fln nts: ATGGGAGACGGGGCTTTCGTCGTGACCTTGGCTGTCACTCAAATATGCAATTCTCGATGATGATAAGTCTGGGCTTACTTTGCATTTCATGGAAGTGACTGTCAAAGATCCTACTGTGGGAGGTACAAGTGAGGGAAGGAGTAATGGAGAATTAGATCCAAACTCATTTTCCGAGTCTAGTTCTGTGAATGATCGAGCTCCATTGCAGTTTATCTTTGAAACTGAAGTTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGGAAGCCATATTGGGCTTGCCAAATTGTTTGCTTCTACCTACCGGATTTCTAGTGATCATGGTCAATATCTCTCTACCAAACTGGAACCTGGGAAATCTATCAAGCTTAAATCCAATGAGACAGTACTCCAGGTTCAATGGCAGGAAACTCGGACAGGGCAAGTTGCAGGAATTTTGACAACACATAGAGTCATGATTGCATCTGCAAATATTGAGATTTTATCATGCAGTTCAACCAGCTTTGATAAGGGTTTTCCTCCATTCAGATCTTTGTTGTGGGTTGGTCCTGCACTTCTATTTTCCACTGCAACTGCAGTAGGGATTCTTGGATGGGATGGGCTAGTACGGACTGTTGTTACCATTAATACACCATATGCAGCACTGGTTGGTACATTGAATGACCGCTTATTATTGGCATGCACAATGGATGCAAACCCTCGGCAGAAGCAATCAGTGGAGATCAAAACTCGCCTTGTGGGACTTCTTGAACCTCTTCTGATTGGATGGGCTACGATGCAACAAAAATTTGAGTCAAAGCTTAATCTTTCAGAGATACTGTACCAGCTTACCTCCAGGTTTGATAGTCTACGAxxxTTTACTCCAAGGTCAGTTGATGTCCTTGCAAATGGTCCTCCAGTTTGTGGAGATCTTGCTGTGGCCTTGGCACAAGCTGGTCCTCAGTTTACCCAGGTGCTGCGGTGCAACTATGCAATTAAATCACACCGCTTTTCCATGGCTCTATCAGTATTGAAGGATGAATTCTTGCGTTCAAGGGATTATCCACAGTGCCCCCCAACTTCTCGGCTGTTCCAACGTTTTCGGCAACTGGGGCAAGCCTGTATCAAGTATGGTCAGTTTGACAGTGCAAAGGAAACATTTGAGGTCATCTCTGATTTTGAGAGCATGCTTGATTTATTCATCTGCCATCTAAATCCTAGTGCTTTGAGACGCCTTGCACAGAAGTTGGAAGATATGGATGCAGAATCTGAACTGAGGCGCCAGTGTGAAAGGATTCTTAGAGTTCGTTCAAGTGGATGGACACAAGGAATATTTGCAAATTTTGCAGCAGAAAGTATGGTCCCAAAGGGTCCAGAATGGGGAGGAGGCAACTGGGAGATCAAGACTCCTCCAGAGTCCAAGAATATACCACAATGGGAACTCTCTGGAGAAGTCATGCCATACATGAAGACTTCTGCTGGGTTCATTCCATCCATTATTCCTGATCATATTGGAGTCTACCTGGGCACAATGAAAGGTAGGGGCACCATTATAGATGTTAGAGATGACACTTCACTGAAGAGATTCACTCAAAGCGTGAATGGTGAAAGCAAGACTGAAGAATTACCTCTGGCTTTGATTAATTCTGAATCAAGCAACTTGAAGAGATCTGATGACGGAATTGTGAAAGGACCTCCTGCAACTGCTTCACAGAGCCTTAGTAAGGCATTCCAAGGAGATGCTGCTGCAGATAAGCAGGCAAGAGCTGCAGAAGAATTTAAGAAGCCCCTATATGGAGGTGATGATAGTAGCAGTGAGGAGGATGATACATCATCAAACC

Fln Alignment:

sp_v1.1_unigene17563___KYAILDDDKSGLTLHFMEVTVKDPTVGGTSE--GRSNGELDPNSFSESSSVNDRAPLQFIFETEVExxxxxxxxxxxxxxxxTGSHIGLAKLFASTYRISSDHGQYLSTKLEPGKSIKLKSNETVLQVQWQETRTGQVAGILTTHRVMIASANIEILSCSSTSFDKGFPPFRSLLWVGPALLFSTATAVGILGWDGLVRTVVTINTPYAALVGTLNDRLLLACTMDANPRQKQSVEIKTRLVGLLEPLLIGWATMQQKFESKLNLSEILYQLTSRFDSLRLxTPRSVDVLANGPPVCGDLAVALAQAGPQFTQVLRCNYAIKSHRFSMALSVLKDEFLRSRDYPQCPPTSRLFQRFRQLGQACIKYGQFDSAKETFEVISDFESMLDLFICHLNPSALRRLAQKLEDMDAESELRRQCERILRVRSSGWTQGIFANFAAESMVPKGPEWGGGNWEIKTPPESKNIPQWELSGEVMPYMKTSAGFIPSIIPDHIGVYLGTMKGRGTIIDVRDDTSLKRFTQSVNGESKTEELPLALINSESSNLKRSDDGIVKGPPATASQSLSKAFQGDXXXXXXXXXXEEFKKPLYG
B9S8J3_______________QFAFLDEDKTGLALYILP--------GGASKAAGEKNLLVEENQSVETNANSLRGPMQFMFESEVDxxxxxxxxxxxxxxxx-GSQIGLAKLLQG-YRLPTSDGHYIPTKTEGKKSIKLKKNEIVLQVHWQETARGYVAGILTTQRVLMVSADLDILASSSTKFDKGRPSFRSLLWVGPALLFSTATAVRVLGWDGIVRTIVSISMPYAVLIGALNDRLLFANPTEINPRQKKGVEIRSCLVGLLEPLLIGFATMQQTFEQKLDLSEVLYQITSRFDSLRIxTPRSLDILARGPPVCGDLAVSLSQAGPQFTQVLRGIYAIKALRFATALSVLKDEFLRSRDYPKCPPTSQLFHRFRQLGYACIKYGQFDSAKETFEVIADYESMLDLFICHLNPSAMRRLAQKLEDEGADPELRRYCERILRVRSSGWTQGIFANFAAESMVPKGPEWGGGNWEIKTPTNLKSIPQWELAAEVMPYMKTDDGTVPAIITDHIGVYLGSIKGRGNVVEVREGSLVKAFKSAV--DDKPNGLPNPLAKSSSNESKGLHEGNSKGDSLMGLETLIKQNASSSAADEQAKAQEEFKKTMYG