SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene17381

Length: 1708 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene17381

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Protein kinase APK1B, chloroplast precursor, putative (EC:2.7.10.2)	-	-	80%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	69%
Blast2go	protein kinase chloroplast	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	Non-specific protein-tyrosine kinase.	EC:2.7.10.2	-	0.0	87%
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	87%

Source	GOs	Term	Type	Identity
Blast2go	auxin biosynthetic process	GO:0009851	Biological Process	87%
Blast2go	protein phosphorylation	GO:0006468	Biological Process	87%
Blast2go	non-membrane spanning protein tyrosine kinase activity	GO:0004715	Molecular Function	87%
Blast2go	ATP binding	GO:0005524	Molecular Function	87%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	87%
Blast2go	plasma membrane	GO:0005886	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Blast2go	Protein kinase, catalytic domain	IPR000719	-	87%
Blast2go	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	87%
Blast2go	Serine/threonine-protein kinase, active site	IPR008271	-	87%
Blast2go	Protein kinase-like domain	IPR011009	-	87%
Blast2go	Serine/threonine-protein kinase-like domain	IPR017442	-	87%
Blast2go	Tyrosine-protein kinase, catalytic domain	IPR020635	-	87%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKU3

Fln msg: Warning!, your query overlaps and the subject is separated, Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 350 - 440

Fln protein: GFH*STHPPSPSTTHKPIPAESNGGNSSPHEVEDLRQNSGSDAYVFTFNELKTITKNFRVHHELGEQAFGTVYKGHIDEDLKPGLKAQPVAVKVLDPEGHQGHREWLAEVIFLGKLSHPHLVKLIGYCCxxxKIALGAAKGLAVLHGAEKPVIYRDFKTSNILLDTDYTAKLSDFGLAKGGPEGDQTHVSTRVMGTYGYAAPEYVMTGHLTARSDVYSFGVVLLELLTGRRSMDKTRPSREYNLAEWARPLLNDKRKLLRILDPRMEGEYSVKGAQKAANLAYHCLSQNPKARPLMRDVVETLEPLQIMDMENTSFTYSVGSGVTTYEVPSQSPGNDVQANDSKHHPDE*

Protein Length: 351

Fln nts: GGGTTTCCATTAATCAACCCACCCGCCATCCCCGTCAACAACCCACAAGCCAATTCCAGCAGAGAGCAATGGAGGAAATTCATCCCCACATGAGGTTGAAGACCTGAGGCAGAACTCTGGTTCCGATGCGTATGTATTCACATTTAATGAGCTCAAGACAATTACCAAAAACTTCAGGGTGCACCATGAACTGGGCGAACAAGCATTTGGTACAGTTTACAAGGGTCACATTGATGAGGACCTGAAGCCAGGCCTCAAGGCCCAGCCAGTTGCTGTCAAGGTCCTTGATCCGGAAGGTCACCAGGGGCACAGAGAATGGCTGGCAGAGGTCATCTTTCTAGGGAAGCTGAGTCATCCCCATTTGGTGAAACTCATTGGATACTGTTGCxxxxxxxxxAAGATTGCACTCGGGGCTGCCAAAGGGCTAGCTGTTCTTCATGGGGCAGAAAAGCCTGTTATCTATCGTGATTTCAAGACGTCCAATATACTACTAGACACAGACTATACAGCAAAGCTTTCAGACTTTGGACTTGCAAAAGGGGGACCAGAAGGGGACCAGACTCATGTTTCCACTCGCGTGATGGGTACATATGGTTATGCAGCTCCTGAGTATGTCATGACTGGTCATTTGACAGCCCGAAGTGATGTATACAGCTTTGGTGTTGTATTGCTTGAGCTGCTTACTGGCAGGCGGTCTATGGATAAGACTAGGCCTAGCAGAGAATATAACTTGGCTGAGTGGGCTCGTCCACTTCTCAACGACAAGCGGAAGTTGTTACGGATACTTGATCCTAGAATGGAGGGAGAGTATTCTGTCAAGGGAGCTCAGAAAGCTGCTAACTTGGCCTATCACTGTCTGAGCCAAAATCCTAAAGCAAGGCCACTTATGAGGGATGTTGTTGAGACGTTGGAACCTCTGCAAATTATGGATATGGAAAACACTTCTTTCACATATTCTGTCGGTAGTGGTGTGACAACATATGAGGTTCCCAGTCAGTCACCAGGAAATGATGTCCAAGCAAATGACTCCAAGCATCATCCAGATGAATAACTTGCTGTTTTAAAAGAAGTTGAAATGTTGCAAGATTGGTCCTGATTCATATAACCAATCTTGGAAGCAACCATATAAACGATTATAAATGCGTGGATGTGCAGAGTTTCCCATTCCTCTGAAGAGTATTATCTCTTCCTGTTGTATGATGTAAACTACTTACAGCATTTCCATTACATCAACTCATTAATCTGCTGAAGATCCCCTTCAACTTTCATAGACAATAATGTCATATCTTGATCACGTCGGTCATGAATGGCTTATTGCACTCGGAATAAAATGAAAGTACTTATCTTTGCTGACTTAGTAGGCAAGCGACATGATACTTGTAAACTGCAAGTTTGCACATCGTTCTCGGATCACAGTGCGGCCCTAGTCAATCATTTTATTTTTTTCAGAAAGTGGTAGATTATTTGATCCTTCATCTGCTATCTGTAAATTATAAATTTTGTATTTGCAACTGCTAGATAAATACGAGTAGCTTTGTATTATCTGCAGAGTATAGTTTTCCATGAAATGAACTGTGGTGGAGGCTTCACATTCAATTCTACAAATACAAGTTGCCATGGACAACTGGTTTGGGACTGCCCATGAAAGGCCATTTTCTGTATTTGAGCTAGAACGTTTTACTGTTAATAAGCATTACAATTTGCTAGAC

Fln Alignment:

sp_v1.1_unigene17381___SSPHEVEDLRQNSGSDAYVFTFNELKTITKNFRVHHELGEQAFGTVYKGHIDEDLKPGLKAQPVAVKVLDPEGHQGHREWLAEVIFLGKLSHPHLVKLIGYCCEExxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRMKIALGAAKGLAVLHGAEKPVIYRDFKTSNILLDTDYTAKLSDFGLAKGGPEGDQTHVSTRVMGTYGYAAPEYVMTGHLTARSDVYSFGVVLLELLTGRRSMDKTRPSREYNLAEWARPLLNDKRKLLRILDPRMEGEYSVKGAQKAANLAYHCLSQNPKARPLMRDVVETLEPLQ-IMDMENTSFTYSVGSGVTTYEVPSQSPGNDVQANDSKHH
B8LKU3_______________STPRAVEDLRQDSGSNVLVFTLFELEIITKSFRSDYILGEGGFGTVYKGYIDENVRAGLKPLPVAVKVLNKNGFQGHREWLTEVIFLGQLSHPHLVKLIGYCCEDxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRMMIALGAAKGLAFLHGAEKPVIYRDFKTSNILLDSDYAAKLSDFGLAKDGPQGDETHVSTRVMGTYGYAAPEYVMTGHLTARSDVYSFGVVLLELLTGRRSVDKTRPNREHNLVDWARPLLNDKRRLLQIIDPRLEGQYSMKGAQKACSLAYHCLNQNPKARPLMGDVVETLEPLQNRKDMVNASLMYTLNNGIAAYKMQNRLKENGSNINGCHVH

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
289	11		88		0.923564
325	10		90		0.90298
342	70		30		1.0
1230	80		20		0.995493
1275	85	14			0.993533
1276	85		14		0.993533
1278	85	14			0.993533
1279	85		14		0.993533
1282		14		85	0.993533
1284	85			14	0.993533
1286	85		14		0.993533
1287			14	85	0.993533
1291		14	85		0.993533
1407		16		83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain