SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene17319

Length: 2756 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene17319
CGTGAATCATTGTCATTTTGGGTGAAAGCCTTCTCTGAGGATACTGCTAGGAGGCTTGAAAACTTCGGCTTTATACAGTTTAGACCTGGAGGTGAGTATCACGGCAATAATCCAGAGATGTCAAAATTGTTACATAAAGCTGTTCGTGAGAAAAATGAAAGCGCATTTTCAGTCTACCAACAACACCTCGCTAACCGGCCAGTAAATGTCCTTCGTGACCTCCTAGAATTTGCAAGTGATCGGCCTTCTATTCCTGTTGGGAAGGTCGAACCAGCTTCAGAAATTGTGCAGCGCTTTTGCACTGGGGGCATGTCACTTGGTGCTATTTCAAGGGAAACACATGAATCGATTGCCGTGGCAATGAACAGAATAGGAGGAAAATCAAATTCAGGGGAAGGTGGTGAGGATCCTGTGCGTTGGAGACCTCTATCAGATGTAGTTGATGGATATTCATCAACATTTCCACACCTTAAAGGTCTTCGAAATGGTGACACTGCCACTAGTGCAATTAAACAGGTTGCTTCAGGTCGGTTTGGTGTTACTCCAACATTTCTAGTTAATGCAGATCAGTTGGAGATCAAGATTGCACAAGGAGCAAAGCCAGGCGAAGGAGGGCAACTGCCTGGAAAGAAAGTCAGCACTTACATAGCAAGATTGCGTAACTCTAAACCTGGTGTACCTCTGATATCACCTCCTCCACATCATGATATCTACTCTATCGAGGACCTTGCGCAATTGATTTTTGACCTTCACCAGGTCAATCCAACGGCAAAGGTTTCTGTCAAGCTTGTAGCAGAGGCTGGAATTGGAACTGTTGCCTCTGGAGTTGCAAAAGGCAATGCTGATATAATACAGATATCAGGACATGATGGGGGCACTGGAGCAAGTCCAATAAGTTCTATCAAACACGCAGGTGGTCCTTGGGAGCTTGGTCTTACAGAGACACATCAGACACTTATTGAGAATGGGCTGCGCGAAAGGGTTGTTCTAAGGGTTGATGGTGGTTTCAAGAGTGGAGTTGATGTCATGTTGGCAGCAGCAATGGGTGCAGATGAGTATGGATTTGGTTCAGTAGCCATGATAGCTACCGGTTGTATTATGGCTAGGATCTGCCACACGAACAATTGTCCTGTTGGTGTTGCTAGCCAGAGAGAGGAATTGCGGGCACGGTTTCCTGGTGTACCTGGAGACTTGGTCAACTTTTTCTTGTATGTTGCAGAAGAGGTTAGAGGTATTCTTGCACAGCTGGGTTATGCGAAGTTGGATGATATTATTGGAAGAACAGATTTGCTAAGACCAAGGCACATATCTCTCATGAAGACTCAGCAACTGGATCTCAGTTATCTCCTTTCTAATGTTGGACGACCAAAATGGAGCAGCACAAAGATACGACAACAAAACGTTCATACAAATGGGCCTGTTCTAGATGACATTTTGTTGGCGGATCCTCAGATTTCAGATGCAATAGAGAAGGAAAGGGAAGTGAAGAAAACTGTTCAAATATACAATGTAGACCGTTCAGTCTGTGGTCGAATTGCTGGTGTCATTGCTAAAAAATATGGAGATACAGGTTATGCAGGACGACTGGAGATACTATTTACTGGCAGTGCAGGGCAGTCCTTTGGATGTTTTTTGACACCTGGCATGAATATTCGGCTTGTAGGAGAGGCAAATGATTATGTTGCCAAGGGCATGGCTGGTGGAGAAATTGTCATAACTCCAAGTGACAAAACGGGTTTTGTACCTGAGGATGCAACTATAGTTGGGAATACTTGCTTATATGGTGCAACAGGTGGTCGACTCTTTGTCCGAGGAAAAGCAGGTGAGCGTTTCGCGGTACGAAATTCTCTCGCTGAAGCCATTGTTGAAGGGACTGGTGATCATTGCTGCGAGTACATGACTGGTGGTTGTGTAGTTGTCCTTGGAAAAGTGGGTCGCAATGTTGCTGCTGGTATGACTGGTGGACTAGCATACCTTCTTGATGAAGATGATACTCTGATTCCAAAGGTAAACAAAGAGATAGTAAAGATCCAAAGAGTAGTGGCTCCTGTTGGTCAACTACAACTAAAACAACTAATAGAGGCTCACGTGGACAAGACTGGAAGTACCAAGGGCACTGCAGTTCTGAAAGAATGGGAAAAATATTTACCTTTGTTTTGGCAACTTGTACCGCCCAGTGAAGAAGACTCCCCTGAAGCCAATCCTGAGTTTGGAGACCGAACTGTTGAAAGGATTACTGTGAAGTCTGCTTAAACTGAAGATACTATCAATGCTGATATATTGCTATTATCAGCAAGTAATCATTTGTTGCCATAGTGTGAGCGAAAAAAATTGTCGCTCTTTTGTAATTATTCTGTACATCCTCTAGACCTGGAGACCATCGTCAGAATACTCTGCCATCAAGGTTTTAGATGCAAGGAAGACAGACTTGAGATGCATAGCAGAAGCACAATTACATTCTATCAACTCCATTACTGATTCCGTTATAAAGTTTTGAATGGCAGTTTATTGTCACTGCATGTAAAGTTCTAGCAGTGATCATATCATTACTTCTCCATGATGTTATGTGCTCAATCATTGTATTGTAATAATGACTTAATGCTCTTGGGTCTCTAGTCAATTGGCATTTTATATATTGCCAAGCATCTGTGGCCCTAGCACCCATTTGTTTTTTAACTGGAATCAAAATATGGAAAGATAAGGTCTAGCATGTATTTTGAGAAGCCTCGAGTACATGAATTTTCTCCTTTCTAAAA

Ace file of the UniGene sp_v1.1_unigene17319 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	glutamate synthase (ferredoxin); K00284 glutamate synthase (ferredoxin) [EC:1.4.7.1]	-	-	87%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	ferredoxin-dependent glutamate synthase	-	-	93%

Source	ECs	Term	Type	e value	Identity
Blast2go	Glutamate synthase (ferredoxin).	EC:1.4.7.1	-	0.0	93%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Nitrogen metabolism	00910		0.0	93%

Source	ECs	Term	Type	e value	Identity
AutoFact	Glutamate synthase (ferredoxin).	EC:1.4.7.1	-	0.0	87%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Nitrogen metabolism	00910		0.0	87%

Source	GOs	Term	Type	Identity
Blast2go	response to light stimulus	GO:0009416	Biological Process	93%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	93%
Blast2go	glutamine metabolic process	GO:0006541	Biological Process	93%
Blast2go	glutamate biosynthetic process	GO:0006537	Biological Process	93%
Blast2go	photorespiration	GO:0009853	Biological Process	93%
Blast2go	positive regulation of glycine hydroxymethyltransferase activity	GO:0080114	Biological Process	93%
Blast2go	glutamate synthase (ferredoxin) activity	GO:0016041	Molecular Function	93%
Blast2go	metal ion binding	GO:0046872	Molecular Function	93%
Blast2go	3 iron, 4 sulfur cluster binding	GO:0051538	Molecular Function	93%
Blast2go	protein binding	GO:0005515	Molecular Function	93%
Blast2go	mitochondrion	GO:0005739	Cellular Component	93%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	93%
Blast2go	membrane	GO:0016020	Cellular Component	93%
Blast2go	apoplast	GO:0048046	Cellular Component	93%

Source	InterPros	Term	Type	Identity
Blast2go	Glutamate synthase, alpha subunit, C-terminal	IPR002489	-	93%
Blast2go	Glutamate synthase, central-C	IPR002932	-	93%
Blast2go	Aldolase-type TIM barrel	IPR013785	-	93%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NSR4

Fln msg: your sequence is longer than subject: 712 - 202

Fln protein: MSKLLHKAVREKNESAFSVYQQHLANRPVNVLRDLLEFASDRPSIPVGKVEPASEIVQRFCTGGMSLGAISRETHESIAVAMNRIGGKSNSGEGGEDPVRWRPLSDVVDGYSSTFPHLKGLRNGDTATSAIKQVASGRFGVTPTFLVNADQLEIKIAQGAKPGEGGQLPGKKVSTYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIFDLHQVNPTAKVSVKLVAEAGIGTVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIENGLRERVVLRVDGGFKSGVDVMLAAAMGADEYGFGSVAMIATGCIMARICHTNNCPVGVASQREELRARFPGVPGDLVNFFLYVAEEVRGILAQLGYAKLDDIIGRTDLLRPRHISLMKTQQLDLSYLLSNVGRPKWSSTKIRQQNVHTNGPVLDDILLADPQISDAIEKEREVKKTVQIYNVDRSVCGRIAGVIAKKYGDTGYAGRLEILFTGSAGQSFGCFLTPGMNIRLVGEANDYVAKGMAGGEIVITPSDKTGFVPEDATIVGNTCLYGATGGRLFVRGKAGERFAVRNSLAEAIVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGMTGGLAYLLDEDDTLIPKVNKEIVKIQRVVAPVGQLQLKQLIEAHVDKTGSTKGTAVLKEWEKYLPLFWQLVPPSEEDSPEANPEFGDRTVERITVKSA*

Protein Length: 713

Fln nts: CGTGAATCATTGTCATTTTGGGTGAAAGCCTTCTCTGAGGATACTGCTAGGAGGCTTGAAAACTTCGGCTTTATACAGTTTAGACCTGGAGGTGAGTATCACGGCAATAATCCAGAG_-_ATGTCAAAATTGTTACATAAAGCTGTTCGTGAGAAAAATGAAAGCGCATTTTCAGTCTACCAACAACACCTCGCTAACCGGCCAGTAAATGTCCTTCGTGACCTCCTAGAATTTGCAAGTGATCGGCCTTCTATTCCTGTTGGGAAGGTCGAACCAGCTTCAGAAATTGTGCAGCGCTTTTGCACTGGGGGCATGTCACTTGGTGCTATTTCAAGGGAAACACATGAATCGATTGCCGTGGCAATGAACAGAATAGGAGGAAAATCAAATTCAGGGGAAGGTGGTGAGGATCCTGTGCGTTGGAGACCTCTATCAGATGTAGTTGATGGATATTCATCAACATTTCCACACCTTAAAGGTCTTCGAAATGGTGACACTGCCACTAGTGCAATTAAACAGGTTGCTTCAGGTCGGTTTGGTGTTACTCCAACATTTCTAGTTAATGCAGATCAGTTGGAGATCAAGATTGCACAAGGAGCAAAGCCAGGCGAAGGAGGGCAACTGCCTGGAAAGAAAGTCAGCACTTACATAGCAAGATTGCGTAACTCTAAACCTGGTGTACCTCTGATATCACCTCCTCCACATCATGATATCTACTCTATCGAGGACCTTGCGCAATTGATTTTTGACCTTCACCAGGTCAATCCAACGGCAAAGGTTTCTGTCAAGCTTGTAGCAGAGGCTGGAATTGGAACTGTTGCCTCTGGAGTTGCAAAAGGCAATGCTGATATAATACAGATATCAGGACATGATGGGGGCACTGGAGCAAGTCCAATAAGTTCTATCAAACACGCAGGTGGTCCTTGGGAGCTTGGTCTTACAGAGACACATCAGACACTTATTGAGAATGGGCTGCGCGAAAGGGTTGTTCTAAGGGTTGATGGTGGTTTCAAGAGTGGAGTTGATGTCATGTTGGCAGCAGCAATGGGTGCAGATGAGTATGGATTTGGTTCAGTAGCCATGATAGCTACCGGTTGTATTATGGCTAGGATCTGCCACACGAACAATTGTCCTGTTGGTGTTGCTAGCCAGAGAGAGGAATTGCGGGCACGGTTTCCTGGTGTACCTGGAGACTTGGTCAACTTTTTCTTGTATGTTGCAGAAGAGGTTAGAGGTATTCTTGCACAGCTGGGTTATGCGAAGTTGGATGATATTATTGGAAGAACAGATTTGCTAAGACCAAGGCACATATCTCTCATGAAGACTCAGCAACTGGATCTCAGTTATCTCCTTTCTAATGTTGGACGACCAAAATGGAGCAGCACAAAGATACGACAACAAAACGTTCATACAAATGGGCCTGTTCTAGATGACATTTTGTTGGCGGATCCTCAGATTTCAGATGCAATAGAGAAGGAAAGGGAAGTGAAGAAAACTGTTCAAATATACAATGTAGACCGTTCAGTCTGTGGTCGAATTGCTGGTGTCATTGCTAAAAAATATGGAGATACAGGTTATGCAGGACGACTGGAGATACTATTTACTGGCAGTGCAGGGCAGTCCTTTGGATGTTTTTTGACACCTGGCATGAATATTCGGCTTGTAGGAGAGGCAAATGATTATGTTGCCAAGGGCATGGCTGGTGGAGAAATTGTCATAACTCCAAGTGACAAAACGGGTTTTGTACCTGAGGATGCAACTATAGTTGGGAATACTTGCTTATATGGTGCAACAGGTGGTCGACTCTTTGTCCGAGGAAAAGCAGGTGAGCGTTTCGCGGTACGAAATTCTCTCGCTGAAGCCATTGTTGAAGGGACTGGTGATCATTGCTGCGAGTACATGACTGGTGGTTGTGTAGTTGTCCTTGGAAAAGTGGGTCGCAATGTTGCTGCTGGTATGACTGGTGGACTAGCATACCTTCTTGATGAAGATGATACTCTGATTCCAAAGGTAAACAAAGAGATAGTAAAGATCCAAAGAGTAGTGGCTCCTGTTGGTCAACTACAACTAAAACAACTAATAGAGGCTCACGTGGACAAGACTGGAAGTACCAAGGGCACTGCAGTTCTGAAAGAATGGGAAAAATATTTACCTTTGTTTTGGCAACTTGTACCGCCCAGTGAAGAAGACTCCCCTGAAGCCAATCCTGAGTTTGGAGACCGAACTGTTGAAAGGATTACTGTGAAGTCTGCTTAA___ACTGAAGATACTATCAATGCTGATATATTGCTATTATCAGCAAGTAATCATTTGTTGCCATAGTGTGAGCGAAAAAAATTGTCGCTCTTTTGTAATTATTCTGTACATCCTCTAGACCTGGAGACCATCGTCAGAATACTCTGCCATCAAGGTTTTAGATGCAAGGAAGACAGACTTGAGATGCATAGCAGAAGCACAATTACATTCTATCAACTCCATTACTGATTCCGTTATAAAGTTTTGAATGGCAGTTTATTGTCACTGCATGTAAAGTTCTAGCAGTGATCATATCATTACTTCTCCATGATGTTATGTGCTCAATCATTGTATTGTAATAATGACTTAATGCTCTTGGGTCTCTAGTCAATTGGCATTTTATATATTGCCAAGCATCTGTGGCCCTAGCACCCATTTGTTTTTTAACTGGAATCAAAATATGGAAAGATAAGGTCTAGCATGTATTTTGAGAAGCCTCGAGTACATGAATTTTCTCCTTTCTAAAA

Fln Alignment:

sp_v1.1_unigene17319___MNIRLVGEANDYVAKGMAGGEIVITPSDKTGFVPEDATIVGNTCLYGATGGRLFVRGKAGERFAVRNSLAEAIVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGMTGGLAYLLDEDDTLIPKVNKEIVKIQRVVAPVGQLQLKQLIEAHVDKTGSTKGTAVLKEWEKYLPLFWQLVPPSEEDSPEANPEFGDRTVERITVKSA
A9NSR4_______________MNIRLLGEANDYVAKGMAGGEIVITPSEKTGFVPEDATIVGNTCLYGATGGRLFVRGKAGERFAVRNSLAEAVVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGMTGGLAYLLDEDDTLVPKVNKEIVKIQRVVAPVGQLQLKQLIEAHVNKTGSTKGTAVLKEWEKYLPLFWQLVPPSEEDTPEANAEFGDRTVERITVKSA

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
413		88		11	0.923564
414	77		11		0.968132
416	87		12		0.992554
417	87			12	0.992554
420		12	87		0.992554
421	12	87			0.992554
423	87	12			0.992554
425	87			12	0.992554
428		14	14	71	0.984337
429	14	14		71	0.984337
430	14	14	14	57	0.979185
431	14			85	0.993533
432		16	16	66	0.988813
433	16		16	66	0.988813
434	16	83			0.994512
440	11	88			0.923564
441	88		11		0.923564
442		88	11		0.923564
445		11		88	0.923564
447	11		88		0.923564
449	88		11		0.923564
457	87	12			0.992554
458		12		87	0.992554
459		12		87	0.992554
477	42			57	1.0
640	88		11		0.999952
1567	16		83		0.994512
1754	14	85			0.993533
1918	75		25		0.996473
2157		50		50	1.0
2161			25	75	0.996473
2167		75		25	0.996473
2169	25			75	0.996473
2170			25	75	0.996473
2639	75			25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain