SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene16801

Length: 1726 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene16801 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	aconitase, putative (EC:4.2.1.3); K01681 aconitate hydratase 1 [EC:4.2.1.3]	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	at4g26970 f10m23_310	-	-	78%

Source	ECs	Term	Type	e value	Identity
Blast2go	Aconitate hydratase.	EC:4.2.1.3	-	0.0	78%

Source	KEGGs	Term	Identity
Blast2go	Citrate cycle (TCA cycle)	00020	78%
Blast2go	Glyoxylate and dicarboxylate metabolism	00630	78%
Blast2go	Carbon fixation pathways in prokaryotes	00720	78%
Blast2go	Biosynthesis of phenylpropanoids	01061	78%
Blast2go	Biosynthesis of terpenoids and steroids	01062	78%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	78%
Blast2go	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064	78%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	78%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	78%
Blast2go	Biosynthesis of plant hormones	01070	78%
Blast2go	Metabolic pathways	01100	78%
Blast2go	Biosynthesis of secondary metabolites	01110	78%

Source	ECs	Term	Type	e value	Identity
AutoFact	Aconitate hydratase.	EC:4.2.1.3	-	0.0	73%

Source	KEGGs	Term	Identity
AutoFact	Citrate cycle (TCA cycle)	00020	73%
AutoFact	Glyoxylate and dicarboxylate metabolism	00630	73%
AutoFact	Carbon fixation pathways in prokaryotes	00720	73%
AutoFact	Biosynthesis of phenylpropanoids	01061	73%
AutoFact	Biosynthesis of terpenoids and steroids	01062	73%
AutoFact	Biosynthesis of alkaloids derived from shikimate pathway	01063	73%
AutoFact	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064	73%
AutoFact	Biosynthesis of alkaloids derived from histidine and purine	01065	73%
AutoFact	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	73%
AutoFact	Biosynthesis of plant hormones	01070	73%
AutoFact	Metabolic pathways	01100	73%
AutoFact	Biosynthesis of secondary metabolites	01110	73%

Source	GOs	Term	Type	Identity
Blast2go	response to oxidative stress	GO:0006979	Biological Process	78%
Blast2go	glyoxylate cycle	GO:0006097	Biological Process	78%
Blast2go	response to salt stress	GO:0009651	Biological Process	78%
Blast2go	response to abscisic acid stimulus	GO:0009737	Biological Process	78%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	78%
Blast2go	copper ion binding	GO:0005507	Molecular Function	78%
Blast2go	4 iron, 4 sulfur cluster binding	GO:0051539	Molecular Function	78%
Blast2go	ATP binding	GO:0005524	Molecular Function	78%
Blast2go	aconitate hydratase activity	GO:0003994	Molecular Function	78%
Blast2go	cell wall	GO:0005618	Cellular Component	78%
Blast2go	mitochondrion	GO:0005739	Cellular Component	78%
Blast2go	chloroplast	GO:0009507	Cellular Component	78%
Blast2go	plasma membrane	GO:0005886	Cellular Component	78%

Source	InterPros	Term	Type	Identity
Blast2go	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha	IPR001030	-	78%
Blast2go	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3	IPR015931	-	78%
Blast2go	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2	IPR015932	-	78%
Blast2go	Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase	IPR015934	-	78%
Blast2go	Aconitase/isopropylmalate dehydratase	IPR015937	-	78%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NX49

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 458 aas, your sequence is shorter than subject: 507 - 565

Fln protein: MHIIGATALLRATRTSFHPSIARAVSTSNFSSASSPFSPRSQSVAALRGFPTGSQAVKSLNTSSFAGDQSRFRSLLGFHRWNLNHGLRAHARSSAAVLSPVERKMSTMATENAFKHILTGLPKPGGGEFGKYYSLTALNDPRTDRLPYSVRILLESAIRNCDNFQVTKNDVEKILDWENTSPKQVEIPFKPARVILQDFTGVPAVVDLASMRDGIKSLGGDPNKINPLVPVDLVVDHSVQVDVARSENALLANMEYEFNRNTERFAFLKWGSTAFENMLVVPPGSGIVHQVNLEYLGRVVFNTNGLLYPAGVVGFKLTGTLNNGVTATDLVLTVTQILRKHGVVGKFVEFYGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLRLTGRSDETVKMIEAYLRANKMFVDYNEPQIERSYSSYLELDLSSVEPCISGPKRPHDRVTLKEMKSDWHACLDNKVGFKGFAIPKEKQEKVVKFTYNGTPAELRHGDVVIAAITS*

Protein Length: 508

Fln nts: ATGGGGGCAGTAGGGGACGGCAGGCAAGCGCTGTTAGGTTTTTTTTATTCAATTATTTTAGTGGTGTCTTTAGCAGGCGTAGGGCGGGGCGGTTTTAAATACGTCGTGACCTTGGCTGTCACTCAAATTTGTCCCCAAGCCTGGGCGATTAGTGGGGTTTTCCGTGTGGTTGTTGTGTGTTGTGCTTATCCATGAGCCAAT_-_ATGCATATAATCGGCGCCACTGCTCTCCTTAGAGCGACTCGAACCTCGTTCCACCCCTCCATAGCTCGAGCTGTTTCGACATCCAATTTTTCTTCGGCATCTTCCCCGTTTTCTCCTCGATCTCAGTCAGTTGCAGCCCTCAGAGGGTTTCCAACTGGTTCCCAGGCCGTAAAATCTTTAAATACCTCGTCTTTCGCAGGCGATCAGTCGCGGTTTCGGAGTTTGCTAGGGTTTCACCGCTGGAATTTAAATCACGGGCTCAGAGCGCACGCGAGATCATCCGCTGCCGTTCTCAGCCCTGTCGAACGGAAAATGTCCACCATGGCGACTGAGAATGCTTTCAAGCATATTTTGACTGGCCTTCCTAAACCTGGTGGAGGAGAGTTTGGAAAGTATTACAGCCTTACAGCTCTCAATGATCCAAGGACAGATAGACTGCCTTATTCTGTCAGAATTCTTCTCGAGTCAGCAATTCGCAACTGTGATAATTTTCAAGTAACGAAGAATGACGTGGAGAAGATTCTTGATTGGGAGAATACTTCCCCAAAACAAGTTGAAATTCCTTTTAAACCTGCTCGTGTTATTCTGCAGGATTTCACTGGTGTGCCAGCAGTTGTTGATCTCGCTAGCATGCGTGATGGTATTAAAAGTCTCGGTGGTGATCCTAATAAGATCAATCCTTTGGTACCTGTGGACCTTGTTGTTGATCATTCAGTACAAGTAGATGTAGCTAGATCTGAAAATGCATTGCTTGCAAACATGGAGTATGAATTTAATCGTAACACAGAAAGGTTTGCTTTCCTCAAGTGGGGCTCAACTGCTTTTGAGAACATGCTCGTTGTTCCTCCGGGTTCTGGTATTGTACACCAGGTGAATTTGGAATATCTGGGAAGGGTGGTATTTAATACCAATGGTCTACTTTATCCGGCTGGTGTTGTTGGTTTCAAATTAACTGGCACTTTGAACAATGGAGTGACAGCTACGGACTTAGTTCTAACGGTGACTCAAATTTTACGAAAGCATGGTGTGGTTGGTAAATTTGTTGAGTTCTATGGTGATGGCATGGGTGAATTATCACTTGCCGATCGAGCAACTATTGCAAACATGTCCCCAGAGTATGGGGCAACCATGGGATTTTTCCCTGTAGATCATGTTACTCTGCAGTATTTGAGGTTAACAGGTCGCAGTGATGAGACTGTGAAGATGATTGAGGCCTACCTCCGTGCCAACAAAATGTTTGTGGACTATAATGAGCCACAAATAGAGAGGTCTTATTCGTCCTACTTGGAATTGGATCTTTCATCTGTTGAACCTTGTATATCAGGACCAAAACGACCTCATGATCGGGTTACTCTCAAGGAAATGAAATCTGATTGGCATGCTTGCCTAGACAACAAGGTTGGGTTTAAGGGTTTTGCCATTCCGAAAGAGAAACAAGAAAAGGTTGTGAAGTTTACATACAATGGTACACCTGCTGAACTAAGACATGGTGATGTTGTTATAGCGGCTATCACCAGTTGACAAC

Fln Alignment:

sp_v1.1_unigene16801___MIEAYLRANKMFVDYNEPQIERXXXXXXXXXXXSVEPCISGPKRPHDRVTLKEMKSDWHACLDNKVGFKGFAIPKEKQEKVVKFTYNGTPAELRHGDVVIAAITS
A9NX49_______________MIESYLRANKMFVDYNEPQIERTYSSYLELDLSSVEPCISGPKRPHDRVTLKEMKADWHSCLDNKVGFKGFAIPKEKQEKVVKFTYNGTPAELRHGDVVIAAITS

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
1549		93		6	0.999153
1550	90		9		1.0
1569	8		92		0.999718
1593	11			88	0.999937
1597		11	83	5	0.999887
1613		78		21	1.0
1614	71		7		1.0
1616	21	78			1.0
1617	7	71	21		1.0
1618	78		21		1.0
1623		78	21		1.0
1624	78			21	1.0
1625	21	78			1.0
1626			78	21	1.0
1629			78	21	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain