SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene16676

Length: 1520 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene16676

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	calcium-dependent protein kinase, putative (EC:2.7.11.17); K13412 calcium-dependent protein kinase [EC:2.7.11.1]	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	50%
Blast2go	calcium-dependent protein	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go	Calcium/calmodulin-dependent protein kinase.	EC:2.7.11.17	-	0.0	86%

Source	ECs	Term	Type	e value	Identity
AutoFact	Non-specific serine/threonine protein kinase.	EC:2.7.11.1	-	0.0	72%

Source	GOs	Term	Type	Identity
Blast2go	auxin biosynthetic process	GO:0009851	Biological Process	86%
Blast2go	protein phosphorylation	GO:0006468	Biological Process	86%
Blast2go	calmodulin-dependent protein kinase activity	GO:0004683	Molecular Function	86%
Blast2go	protein binding	GO:0005515	Molecular Function	86%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	86%
Blast2go	ATP binding	GO:0005524	Molecular Function	86%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PQ38

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 26 aas, your sequence is shorter than subject: 269 - 529

Fln protein: VVTLAVTQFVVTLAVTHFWAESEQGIFDAILNGHIDFESDPWPSISNSAKDLVRRMLKFEPKERLTAIQVLNHSWIREDGEAPDTPIDNAVLNRMKQFRAMNKLKKVALKVIAENLSEEEIMGLKEMFKSMDTDNSGTITLEELKIGLARQGSKLSETEVRQLMEAADVDGNGSIDYIEFITATMHMNRMERADHIYRAFQYFDKDSSGYITVDELEQALKKYGIDDDRTIKEIIAEVDTDHDGRINYDEFAAMMRKGTPDGNPKKRR*

Protein Length: 270

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTCGTCGTGACCTTGGCTGTCACTCATTTTTGGGCAGAGTCAGAGCAAGGTATTTTCGATGCTATCTTGAATGGACATATTGACTTTGAAAGTGATCCATGGCCATCAATATCTAATAGCGCTAAGGACTTGGTCAGAAGGATGCTCAAGTTTGAACCAAAGGAGCGACTTACAGCTATCCAAGTCTTAAACCACTCTTGGATCAGAGAAGATGGTGAAGCACCAGATACACCTATTGACAATGCAGTTTTGAATAGAATGAAGCAATTCAGAGCAATGAACAAGCTCAAAAAAGTTGCTCTGAAGGTAATTGCTGAAAACTTGTCAGAAGAGGAAATAATGGGTTTGAAGGAGATGTTCAAGAGCATGGACACCGATAACAGTGGCACAATAACTTTGGAAGAGTTAAAGATAGGTCTTGCAAGGCAAGGCTCAAAACTCTCTGAAACTGAAGTCAGGCAACTGATGGAGGCTGCTGATGTAGATGGGAATGGGTCAATTGATTACATTGAGTTTATCACAGCAACGATGCATATGAACAGAATGGAGAGAGCAGATCATATATACAGGGCCTTTCAATACTTTGACAAAGACAGCAGTGGGTATATCACAGTGGATGAGTTGGAACAAGCTTTGAAAAAGTATGGAATAGATGATGACAGAACAATTAAGGAAATTATTGCCGAGGTGGACACAGACCATGATGGACGGATAAACTATGATGAGTTTGCAGCAATGATGAGAAAAGGAACCCCGGACGGAAATCCCAAAAAGAGACGCTGA___GATATTTATATGACGGGGCTCCAAGACATAAATGCTTTCCGAGTAAATCGCTTCTATGCCGTTCTAGGTTCCTGTGATGAGTTCTTTATTGTTGTGATCACAATTGTGATCAGTCACAACATGAACCCATTACATAAATGTTCTCAAGGATAACAAGTTAGAAGTCAGAATTTATGAAATAATTGATTCCCTCTTTTGTACAGCGTGTTCTGGGGGCATTTGCAAAGGTTGTTACAGGGCAACCTCTCCTCCGGGATCATTTAAGCCTTTGCTGCTTGTTTGTTGAGAGAGGGTATTCTTGGTGCATATTAACCATGAAGCTGCAACATGAAGCTAATTTCTTTTTCTAAATCAAAGAGCTTGGCGAGATCATTTTGTTCACTATAGTTGTATTGCAGTTGGCTAATAAAGTGTTTGTAATTATGTTTTGGCCTGATTGCTGTGGAAGGTGTCCAGCCATATTGTAAGGTCGCTCTTTCTTTGGGAATATAAGCGCATCGATGAATCAATGTTGTGGCATATTTTCAAAATGCGTGTTACTATTTCTAATCAGGATTATGGAAGAGTAATAAGTTTTGGTTTTGGAAGCTCTCGTTGGTGGCCAAGTTAGATTAATGATCTGTTAATTTCAGCCCCAATCTTTGAATTTTCTGAATAATGTTATTTGAACCTTCTGTAGCAACATTCCAGAGTCATTCAATATTTCTCTTGA

Fln Alignment:

sp_v1.1_unigene16676___VVTLAVTQFVVTLAVTHFWAESEQGIFDAILNGHIDFESDPWPSISNSAKDLVRRMLKFEPKERLTAIQVLNHSWIREDGEAPDTPIDNAVLNRMKQFRAMNKLKKVALKVIAENLSEEEIMGLKEMFKSMDTDNSGTITLEELKIGLARQGSKLSETEVRQLMEAADVDGNGSIDYIEFITATMHMNRMERADHIYRAFQYFDKDSSGYITVDELEQAL-KKYGIDDDRTIKEIIAEVDTDHDGRINYDEFAAMMRKGT
C0PQ38_______________VWSAGVILYILLCGVPPFWAESEQGVAQAILRGFVDFKREPWPKISETAKSLVRQMLEPDPKRRLTAQQVIEHPWLQNAKKAPNVPLGDVVKTRLKQFSMTNKFKKRALRVIADHLSVEETKGIKDMFKMMDTDNSGSISYDKLKAGLHKLGSQMDESEVQILMDAADADGNGMLDYREFVAASLHMQSIDNDDYLRKAFLFFDKDGSGYIEIEEFREALADDFGPNDIDVVNSIFNEVDADKDGRISYEEFASMMKTGT

SNPs (tot: 48)

Position	A %	C %	G %	T %	Probability
456	33		66		0.997452
601		11		88	0.923564
604	88		11		0.923564
606	88		11		0.923564
607	11	88			0.923564
608			11	88	0.923564
615	88		11		0.923564
618	88	11			0.923564
638			88	11	0.923564
789	83		16		0.994512
825		22	77		0.999999
827		55	22	22	0.999999
828	22			77	0.999999
831	66			33	1.0
832	77			22	0.999999
834	77		22		0.999999
836	77			22	0.999999
838	77	22			0.999999
840	77	22			0.999999
844	22			77	0.999999
850	81			18	0.999994
853	81		18		0.999994
855	69	30			1.0
857		69		30	1.0
859		76	7	15	0.999983
860		7	15	76	0.999983
861	15			84	0.999986
863	7	15		76	0.999983
864	69	15		15	0.999985
867		76	15	7	0.999983
871		15		84	0.999986
966		14		85	0.999981
1048		90		9	0.999872
1049	90		9		0.999872
1054		90		9	0.999872
1055			9	90	0.999872
1056		90	9		0.999872
1062			90	9	0.999872
1063		9	90		0.999872
1162	80		20		1.0
1285		17		82	1.0
1293		12	87		0.999964
1333		10		90	0.90298
1359		88	11		0.923564
1360	88		11		0.923564
1381	20		80		0.995493
1382	20			80	0.995493
1490	66			33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain