SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene16619

Length: 1879 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene16619

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glucan endo-1,3-beta-glucosidase, putative n=1 Tax=Ricinus communis RepID=B9RYW1_RICCO	-	-	57%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	86%
Blast2go	glucan endo- -beta-glucosidase	-	-	77%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Glycosylases, Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds.	EC:3.2.1.-	-	0.0	77%

Source	KEGGs	Term	Identity
Blast2go	Ascorbate and aldarate metabolism	00053	77%
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130	77%
Blast2go	Amino sugar and nucleotide sugar metabolism	00520	77%
Blast2go	Glycosaminoglycan degradation	00531	77%
Blast2go	Metabolic pathways	01100	77%

Source	GOs	Term	Type	Identity
Blast2go	carbohydrate metabolic process	GO:0005975	Biological Process	77%
Blast2go	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	77%
Blast2go	cation binding	GO:0043169	Molecular Function	77%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	77%

Source	InterPros	Term	Type	Identity
Blast2go	Glycoside hydrolase, family 17	IPR000490	-	77%
Blast2go	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	77%
Blast2go	Glycoside hydrolase, superfamily	IPR017853	-	77%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LRW3

Fln msg: Separated hits, possible frame ERROR between 634 and 646,

Fln protein: MGASVMGNGDLRMFCLSALVLLPGLALSNSASSTSSSATGINYGQVADDLPSPEVVVSLLQSRNINKVKIFSANETVLKAFANTGIELIVAIGNENVGNMTDPTKATEWVNENIQAYLPATxxxxxIAVGNEVYTGTDTQLMENLVPAMKNIQSALVSIGADTDIKITTPHSLGVLGNSYPPSAGSFTSDLTSLMKPLLDFLSQIDSPFFVNVYPFFAYKANPSQISLDYVLFQPNAGIVDPNNNISYNNMLYAQVDAVYSALSALGYPNLEVTVSETGWPSMGDADEAGATLQNAQTYNGNLLKLLAQNQGTPLRPEVVLQAYLFALFNEDMKPGPTSERNYGLYKPDGTAVYNLVSTGTLSTASTRTVYTASYPTAADGPSSFAKETYSVVSFYNHLQLLGLSTFFVFLLFRP*

Protein Length: 417

Fln nts: CGTCGTGACCTTGGCTGTCACTCACACTATCACCGGAAATCTTCGTCGGGAAAAGAAAAGCTGATGTCACCCAAGACGGCAGAAGGCCACATTCGGAAATTGACGGGACACCAACAGCAAAAGCCACAGTATTAATTTGTGCTATCCTGGAAAACCCACAAATTGTGAGTATGCACATGTGCCATGCACAGCAACAGCGAGAACCACAGCATTAATTTCTCGCCATTCTTTGCACGCGAGGAGATTTGTAGGTGAGGAGGCTGCTGCA_-_ATGGGAGCTTCAGTAATGGGCAATGGAGACCTCAGAATGTTCTGCTTGTCGGCTCTGGTTTTGCTCCCTGGACTGGCTCTTTCAAATTCTGCGTCCAGCACAAGTTCCTCCGCAACAGGAATCAACTATGGACAAGTTGCAGACGATTTGCCATCACCGGAAGTAGTAGTGAGCCTTTTGCAGTCTAGGAATATCAACAAAGTAAAAATCTTTAGTGCAAATGAGACAGTCCTCAAAGCATTTGCAAACACTGGTATAGAATTGATTGTTGCAATTGGGAATGAAAATGTTGGCAACATGACAGATCCAACTAAAGCCACTGAATGGGTGAATGAAAACATTCAGGCCTACTTACCAGCCACTAAxxxxxxxxxxxxCATAGCAGTGGGGAACGAAGTTTACACAGGGACTGATACACAGTTAATGGAAAACCTGGTTCCTGCAATGAAAAACATTCAGTCGGCCCTTGTCAGCATCGGTGCAGACACGGATATTAAAATTACTACTCCCCATTCTCTTGGTGTACTTGGGAATTCATATCCACCATCTGCTGGTTCCTTTACATCAGACCTGACGAGCCTAATGAAACCACTTTTGGATTTCCTGTCTCAGATTGATTCTCCTTTCTTCGTAAATGTTTATCCATTCTTTGCATACAAAGCTAATCCAAGCCAGATATCCCTCGATTATGTCCTATTCCAACCAAATGCAGGTATTGTTGACCCCAACAATAATATTAGCTATAACAACATGTTATATGCACAGGTTGATGCTGTTTATTCTGCACTGTCTGCACTGGGGTATCCTAATCTAGAAGTCACAGTCTCTGAAACTGGATGGCCTTCAATGGGTGATGCTGACGAAGCAGGTGCAACTCTTCAGAATGCCCAAACTTATAATGGGAATCTACTTAAACTGCTGGCTCAGAACCAAGGGACTCCTTTGAGACCCGAAGTTGTTTTGCAGGCTTACCTATTTGCATTATTCAATGAGGACATGAAGCCTGGCCCAACCTCGGAGAGGAACTATGGGCTCTATAAGCCAGATGGAACAGCAGTTTACAATCTGGTATCAACTGGAACATTGTCTACTGCTTCTACTAGAACTGTGTATACTGCTTCTTATCCCACTGCTGCTGATGGTCCATCCTCTTTTGCCAAGGAAACCTATTCTGTTGTTTCCTTCTACAATCATCTCCAGCTTTTGGGCCTCTCCACTTTCTTTGTTTTTCTACTGTTTCGGCCATGA___TTGGAGCCAGGGAAGTCCATCAGAGATTTACATGTTTCCCTTCATTCGACATCAGCTCCAAATTATGACGAAAATTCAGACGAAATCCTATGACATGCCACTAAATATACAGTGCTGACAGTGAATCAGCAAGCAGCACCTGCATCTATACTAAAACATGACAGCTTGAGCATCCTCACTTATTAACGAGTAACTGTTGTCACATACATGGTGATGGCAATGAATCTCAGATTCATGGTGATGACATTGAATCTCAGATTTGTAACGTTACATTCATCAACATCAGCTCTGTTGCCATTACATGTAGGCAAAATCTTATTCTGCTTCTATAACTCTGTTACCCTATTATGCATCGTTTGATCTC

Fln Alignment:

sp_v1.1_unigene16619___MGASVMGNGDLRMFCXXXXXXXXXXXXXXXXXXXXXXXXGINYGQVADDLPSPEVVVSLLQSRNINKVKIFSANETVLKAFANTGIELIVAIGNENVGNMTDPTKATEWVNENIQAYLPATKxxxxIAVGNEVYTGTDTQLMENLVPAMKNIQSALVSIGADTDIKITTPHSLGVLGNSYPPSAGSFTSDLTSLMKPLLDFLSQIDSPFFVNVYPFFAYKANPSQISLDYVLFQPNAGIVDPNNNISYNNMLYAQVDAVYSALSALGYPNLEVTVSETGWPSMGDADEAGATLQNAQTYNGNLLKLLAQNQGTPLRPEVVLQAYLFALFNEDMKPGPTSERNYGLYKPDGTAVYNLVSTGTLSTASTRTVYTASYPTAADGPSSF---AKETYSVVSF
B8LRW3_______________MGTSVTSNGDLRMRCLWALVLLSGLALSNSASNTSASTTGINYGQVADNLPSPEVVVGLLQANNINKVKLFSANETVLKAFANTGIELIVGIGNENVGNMTDPTKATEWVNENIRTYLPATKxxxxIAVGNEVYTGTDTQLMANLVPAMKNIHSALVSIGADMNIQITTPHSLAVLGNSYPPSAGSFVSDLNSLMKPLLDFLSQIGSPFFINIYPYFAYKSNPSQVSLDYVLFQPNAGVIDPNNNIRYNNMLYAQVDAVYSALSALGYANLEVTVSETGWPSMGDASEAGATLQNAQTYNGNLLQLLAQNQGTPLRPKLVLQAYLFALFNEDMKTGPTSERNFGLFKPDGTAVYNLGLTGTLSTGSTRTV-TASYPNAADGPSSYFSSAKVSFSIIFF

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
922	75		25		0.996473
928	83		16		0.994512
952		83	16		0.994512
983	80		20		0.995493
991		20		80	0.995493
1042			20	80	0.995493
1089		14	85		0.993533
1120		28		71	1.0
1129		14		85	0.993533
1156	12	87			0.992554
1157		87		12	0.992554
1158	87		12		0.992554
1159	12		87		0.992554
1160	87		12		0.992554
1171	16		83		0.994512
1220	23		76		1.0
1241		78		21	1.0
1387	14			85	0.993533
1422		16		83	0.994512
1435		83		16	0.994512
1437	83		16		0.994512
1489	10	90			0.90298
1490		10		90	0.90298
1491			10	90	0.90298
1493	10		90		0.90298
1585		88		11	0.923564
1594	81	18			0.999994
1596	81			18	0.999994
1599	81			18	0.999994
1600	81		18		0.999994
1609	81		18		0.999994
1614		81		18	0.999994
1625	81		18		0.999994
1672	20			80	0.999996
1702	88		11		0.923564
1736	80			20	0.999996

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain