SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene16175

Length: 1997 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene16175
AGCCTTTGTATTTATATAGATCCAAGCTTCCCGATATTGAAATCTCCAGCCATCTTACTCTGCACGATTATTGCTTCGAGAAATTGGGGGAATTTGCAGAGAGGCCATGCATTGTACAGGGATCTAATGGGAAAGTTTATTCCTATGGCGAAGTTGATTTGATTTCTCGCAGGGTCGGCTCTGGTTTGGCCAAGCTCGGCATCAGGCATGGAGATGTCGTTATGCTTTTGCTGCCCAATTGCCCAGAGTTCGCTTTCGTTTTCTTGGGTTCGTCTCGAATTGGCGCCGTTGCGACTACTGCCAATCCTTTCTATACGCCGGGCGAGATTGCGAAGCAGGCAATGGCGTCCCAGGCTCGGGTGATCGTCACTCAGGCTTCGTATGTCGAGAAATTGTGCAATCTCGAGAGGGAAAAAAGTTCAATTCTGGTTATTACAATTGACCCAGGGGCTCCGGAGGGGTGCCTGCATTTCTCGGTTCTGGCAGAGGCCGACGAGAATGAGATACCCGTTGTCGAGATTCGACCTGACGACGTCATGGCTTTGCCCTATTCCTCTGGCACCACCGGGCTTCCCAAGGGCGTCATGCTCACGCACGGGGCGCTCGTTTCTTCTGTTGCGCAGCAGGTGGACGGCGAGAATCCCAATCTGTATCTGCATTGCGAGGACGTGGTTCTGTGCGTGCTGCCGCTGTTTCATATTTATTCTCTCAATTCTGTGCTGCTCTGTTCTCTCAGAGCGGGCGCGGCTGTGTTGATTATGCAGAGATTCGAAATCCATGCGATGCTGGAGCTGATACAGAAATTCAAGGTCACTGTGGCTCCGATCGTGCCTCCGATTGTTCTGCCCATCGCCAAGAATCCAGTAGTAGAAAAATATGATATGTCGTCCATCAGAATGGTCTTGTCAGGCGCTGCGCCTCTCGGGAAGGAGCTGGAAGACGCCCTTAGGGCTAGGATTCCCAACGCAACTTTGGCCCAGGGTTATGGTATGACAGAGGCTGGACCAGTTCTGGCCATGTCTCTGGGTTTCGCAAAAGTGCCCTTTACTATAAAGTCTGGCTCCTGCGGTACCGTGGTTAGAAATGCGGAGATGAAGATAATCGAACCCGAGACCGGAGTCTCCCTGCCACATAACCACCGGGGAGAGATCTGTATCCGCGGCCCTCAAATTATGAAAGGGTATCTGAATGATCCGGAGGCGACTGAGATGACGATTGATAAAGATGGATGGCTGCATACCGGAGACGTTGGTTACATTGACGATGATGAAGAGCTTTTCATAGCGGACCGTGTCAAGGAACTGATCAAATACAAAGGGTTCCAGGTGCCACCGGCGGAACTGGAAGCGATTCTTTTGAGCCATCCATCCATAGCCGACGCAGCAGTTGTGTCGCAGCAGGATGAAATTGCTGGAGAGGTGCCGGTAGCCTTTGTGGTGAGATCTGGTGATGGGATTGATTTAACGGTGAATGAGATTAAAGATTTCGTTGCCAAACAGGTGGTGTTCTACAAGAAGCTGCACAACGTTTATTTTATAGAAGCCATACCCAAATCTCCATCTGGCAAGATACTGCGCAAGGATTTAAGAGCCAAGCTTATGGCTGTCGCTGCAGGCATTCAATAATTTCAGCGGCCATGGCAGAAGGAGCCTTATCTGCTTTTATATTAAAATATATACAGATTTTGTGTATTTTCTGGTGTCTCCCCAGCAGGATTGACCTGGTAGTGTAAGTTGTGAAAGTTTACTTATTCATTTATTTCCTCGTTCATTTTTAGCTGGCCTGAGATGACTCTGGACATGTTTCTAATTATAGTGTTGCTGCTGCCATGGTATGCATGGCATGACATGACAGAGTTGTTGTCAGTTTATTCCATTTTTAAAAATGGGTAAACGGCCTTTGGTTGTTGGTAACACATTCATCGTGTGACGATGAATGGCAAATCAGTGTGACTCAGCTCATGTAATACTGTTTTTTATAAATGTTGGTTCCAAATC

Ace file of the UniGene sp_v1.1_unigene16175

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	4-coumarate--CoA ligase n=4 Tax=Pinaceae RepID=4CL_PINTA	-	-	69%
FL-Next	tr=4-coumarate:CoA ligase; Pinus massoniana.	-	-	72%
Blast2go	4-coumarate: ligase	-	-	84%

Source	ECs	Term	Type	e value	Identity
Blast2go	Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing).	EC:1.13.12.7	-	0.0	84%
Blast2go	4-coumarate--CoA ligase.	EC:6.2.1.12	-	0.0	84%

Source	KEGGs	Term	Identity
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130	84%
Blast2go	Phenylalanine metabolism	00360	84%
Blast2go	Phenylpropanoid biosynthesis	00940	84%
Blast2go	Biosynthesis of phenylpropanoids	01061	84%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	84%
Blast2go	Metabolic pathways	01100	84%
Blast2go	Biosynthesis of secondary metabolites	01110	84%

Source	GOs	Term	Type	Identity
Blast2go	phenylpropanoid metabolic process	GO:0009698	Biological Process	84%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	84%
Blast2go	ATP binding	GO:0005524	Molecular Function	84%
Blast2go	Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity	GO:0047077	Molecular Function	84%
Blast2go	4-coumarate-CoA ligase activity	GO:0016207	Molecular Function	84%

Source	InterPros	Term	Type	e value	Identity
Blast2go	AMP-dependent synthetase/ligase	IPR000873	-	0.0	84%
Blast2go	AMP-binding, conserved site	IPR020845	-	0.0	84%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: C1KBY2

Fln msg: atg_distance in limit (1-15): atg_distance = 8, W2: There is no M at the beginning,

Fln protein: PLYLYRSKLPDIEISSHLTLHDYCFEKLGEFAERPCIVQGSNGKVYSYGEVDLISRRVGSGLAKLGIRHGDVVMLLLPNCPEFAFVFLGSSRIGAVATTANPFYTPGEIAKQAMASQARVIVTQASYVEKLCNLEREKSSILVITIDPGAPEGCLHFSVLAEADENEIPVVEIRPDDVMALPYSSGTTGLPKGVMLTHGALVSSVAQQVDGENPNLYLHCEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLIMQRFEIHAMLELIQKFKVTVAPIVPPIVLPIAKNPVVEKYDMSSIRMVLSGAAPLGKELEDALRARIPNATLAQGYGMTEAGPVLAMSLGFAKVPFTIKSGSCGTVVRNAEMKIIEPETGVSLPHNHRGEICIRGPQIMKGYLNDPEATEMTIDKDGWLHTGDVGYIDDDEELFIADRVKELIKYKGFQVPPAELEAILLSHPSIADAAVVSQQDEIAGEVPVAFVVRSGDGIDLTVNEIKDFVAKQVVFYKKLHNVYFIEAIPKSPSGKILRKDLRAKLMAVAAGIQ*

Protein Length: 542

Fln nts: AGCCTTTGTATTTATATAGATCCAAGCTTCCCGATATTGAAATCTCCAGCCATCTTACTCTGCACGATTATTGCTTCGAGAAATTGGGGGAATTTGCAGAGAGGCCATGCATTGTACAGGGATCTAATGGGAAAGTTTATTCCTATGGCGAAGTTGATTTGATTTCTCGCAGGGTCGGCTCTGGTTTGGCCAAGCTCGGCATCAGGCATGGAGATGTCGTTATGCTTTTGCTGCCCAATTGCCCAGAGTTCGCTTTCGTTTTCTTGGGTTCGTCTCGAATTGGCGCCGTTGCGACTACTGCCAATCCTTTCTATACGCCGGGCGAGATTGCGAAGCAGGCAATGGCGTCCCAGGCTCGGGTGATCGTCACTCAGGCTTCGTATGTCGAGAAATTGTGCAATCTCGAGAGGGAAAAAAGTTCAATTCTGGTTATTACAATTGACCCAGGGGCTCCGGAGGGGTGCCTGCATTTCTCGGTTCTGGCAGAGGCCGACGAGAATGAGATACCCGTTGTCGAGATTCGACCTGACGACGTCATGGCTTTGCCCTATTCCTCTGGCACCACCGGGCTTCCCAAGGGCGTCATGCTCACGCACGGGGCGCTCGTTTCTTCTGTTGCGCAGCAGGTGGACGGCGAGAATCCCAATCTGTATCTGCATTGCGAGGACGTGGTTCTGTGCGTGCTGCCGCTGTTTCATATTTATTCTCTCAATTCTGTGCTGCTCTGTTCTCTCAGAGCGGGCGCGGCTGTGTTGATTATGCAGAGATTCGAAATCCATGCGATGCTGGAGCTGATACAGAAATTCAAGGTCACTGTGGCTCCGATCGTGCCTCCGATTGTTCTGCCCATCGCCAAGAATCCAGTAGTAGAAAAATATGATATGTCGTCCATCAGAATGGTCTTGTCAGGCGCTGCGCCTCTCGGGAAGGAGCTGGAAGACGCCCTTAGGGCTAGGATTCCCAACGCAACTTTGGCCCAGGGTTATGGTATGACAGAGGCTGGACCAGTTCTGGCCATGTCTCTGGGTTTCGCAAAAGTGCCCTTTACTATAAAGTCTGGCTCCTGCGGTACCGTGGTTAGAAATGCGGAGATGAAGATAATCGAACCCGAGACCGGAGTCTCCCTGCCACATAACCACCGGGGAGAGATCTGTATCCGCGGCCCTCAAATTATGAAAGGGTATCTGAATGATCCGGAGGCGACTGAGATGACGATTGATAAAGATGGATGGCTGCATACCGGAGACGTTGGTTACATTGACGATGATGAAGAGCTTTTCATAGCGGACCGTGTCAAGGAACTGATCAAATACAAAGGGTTCCAGGTGCCACCGGCGGAACTGGAAGCGATTCTTTTGAGCCATCCATCCATAGCCGACGCAGCAGTTGTGTCGCAGCAGGATGAAATTGCTGGAGAGGTGCCGGTAGCCTTTGTGGTGAGATCTGGTGATGGGATTGATTTAACGGTGAATGAGATTAAAGATTTCGTTGCCAAACAGGTGGTGTTCTACAAGAAGCTGCACAACGTTTATTTTATAGAAGCCATACCCAAATCTCCATCTGGCAAGATACTGCGCAAGGATTTAAGAGCCAAGCTTATGGCTGTCGCTGCAGGCATTCAATAA___TTTCAGCGGCCATGGCAGAAGGAGCCTTATCTGCTTTTATATTAAAATATATACAGATTTTGTGTATTTTCTGGTGTCTCCCCAGCAGGATTGACCTGGTAGTGTAAGTTGTGAAAGTTTACTTATTCATTTATTTCCTCGTTCATTTTTAGCTGGCCTGAGATGACTCTGGACATGTTTCTAATTATAGTGTTGCTGCTGCCATGGTATGCATGGCATGACATGACAGAGTTGTTGTCAGTTTATTCCATTTTTAAAAATGGGTAAACGGCCTTTGGTTGTTGGTAACACATTCATCGTGTGACGATGAATGGCAAATCAGTGTGACTCAGCTCATGTAATACTGTTTTTTATAAATGTTGGTTCCAAATC

Fln Alignment:

sp_v1.1_unigene16175___YLYRSKLPDIEISSHLTLHDYCFEKLGEFAERPCIVQGSNGKVYSYGEVDLISRRVGSGLAKLGIRHGDVVMLLLPNCPEFAFVFLGSSRIGAVATTANPFYTPGEIAKQAMASQARVIVTQASYVEKLCNLEREKSSILVITIDPGAPEGCLHFSVLAEADENEIPVVEIRPDDVMALPYSSGTTGLPKGVMLTHGALVSSVAQQVDGENPNLYLHCEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLIMQRFEIHAMLELIQKFKXXXXXXXXXXXXXXAKNPVVEKYDMSSIRMVLSGAAPLGKELEDALRARIPNATLAQGYGMTEAGPVLAMSLGFAKVPFTIKSGSCGTVVRNAEMKIIEPETGVSLPHNHRGEICIRGPQIMKGYLNDPEATEMTIDKDGWLHTGDVGYIDDDEELFIADRVKELIKYKGFQVPPAELEAILLSHPSIADAAVVSQQDEIAGEVPVAFVVRSGDGIDLTVNEIKDFVAKQVVFYKKLHNVYFIEAIPKSPSGKILRKDLRAKLMA
C1KBY2_______________HLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYSFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSH--DVLVITIDDAPKEGCQHISVLTEADETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSEDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEETGEVPVAFVVKSS---EISEQEIKEFVAKQVVFYKKIHRVYFVDAIPKSPSGKILRKDLRSRLAA

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
1469			66	33	1.0
1536	80		20		0.995493
1570	10			90	0.90298
1588	11		88		0.923564
1598		11		88	0.923564
1602		11	88		0.923564
1648	88	11			0.923564
1721		83	16		0.994512
1722	33			66	1.0
1792		77	22		0.999999
1812	44			55	1.0
1846	87		12		0.992554
1883	20			80	0.995493
1885	20			80	0.995493
1893	80		20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain