SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene16162

Length: 1266 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene16162 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	MAP7 multi-domain protein	-	-	1.0e-09	27%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	66%
Blast2go	unknown [Picea sitchensis]	-	-	0.0	75%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5ADZ5

Fln msg: Unexpected stop codon in the beginning of your sequence,

Fln protein: KES*ETEA*KRRETCRAPSNSKTGKGVTQKDGIL*KAGGGKVDQAQGRGRSSQA*GGRTKAQTRCRKKAKLDEIAEKQKQREKELEEKERLRKEALVKEEPRVIKPSEPNSTKFVPRALRKAAESVGTEGDNDSWRRLDRPIHIIDRRSFFSDDVKQSTSPAHGLSSRDGNPMPRQREADVVPPREKYAPPGKYEPPTRHTQGKYEAPFSGRRENQYEPPRGRDSQPPIGQRPKYGGFGIRDNDRDRWGGGPDREKQRDEDRSVGADKWRKPQVAT*

Protein Length: 278

Fln nts: CCAAGGAGAGTTGAGAGACTGAAGCGtGAAAGAGAAGAGAGACTTGCAGAGCTCCAAGCAATTCGAAAACAGGAAAGGGAGTTACGCAGAAAGATGGAATTTTATAGAAGGCAGGAGGAGGCAAGGTTGATCAAGCTCAAGGAAGAGGAAGAAGCTCGCAAGCGTGAGGAGGAAGAACTAAAGCGCAAACAAGATGCAGAAAGAAAGCCAAGCTGGATGAGATTGCTGAAAAACAGAAGCAGCGTGAAAAGGAACTGGAGGAGAAGGAGAGGCTACGAAAAGAAGCTTTGGTCAAGGAAGAACCAAGAGTTATAAAGCCCTCAGAGCCAAATTCAACCAAATTTGTACCCCGAGCTCTGCGCAAGGCAGCAGAATCAGTTGGTACTGAAGGAGATAATGATAGTTGGCGACGCCTAGATCGACCAATCCATATTATTGATAGGAGATCATTTTTTTCAGATGATGTTAAACAATCTACTAGTCCAGCTCATGGTTTATCCAGCAGAGATGGGAATCCCATGCCTAGACAACGTGAAGCAGATGTTGTGCCTCCTAGGGAGAAGTATGCTCCACCTGGTAAATATGAGCCACCTACAAGACACACACAGGGTAAATATGAAGCTCCATTCTCTGGAAGGCGTGAAAATCAATATGAGCCACCTCGTGGGCGGGATTCTCAACCCCCTATTGGGCAACGACCCAAGTATGGTGGATTTGGTATTAGGGATAATGATCGTGATCGTTGGGGAGGTGGTCCTGACCGTGAAAAGCAACGTGATGAAGACCGATCTGTAGGTGCTGATAAGTGGAGGAAGCCTCAAGTTGCCACTTAG___GCATGTGGAGGAACAGATTTAGTTTCCACCTAGGCATGTAGAGGAACAGGCTGCTTAAAAGTCATGCAGCTGTCTTTCCTACTGCACTATGGCTTACATGGACCAGGTTTTTTGCTGGCCTTGCCTGGATATTGAGCAGTGGAATTTAGTTGAGTACTCAACTTTGGTGCCGTCAAGTGACACGGAATTGAGATATGTCAGTTTTTGGTTGCAGAGAATATTGGAAGTTTTCAATTTGCCTATGAAGACTATATGTAGAAATAAAAATATAGTTACTTTTAAAATTTTATTCTGGTATTTGAACTAAGTTTTGACCTATATTATTTTTTTAACTTCTTTCTGTTAATTAAGGGAACATACCTCTGGTATCTTGCGTGCAAATTTGTTATGGACATTGCAAGGGATGTACAAATATTCGGGCACCGGTTTTATA

Fln Alignment:

sp_v1.1_unigene16162___KAKLDEIXXXXXXXXXXXXXXXXXXXXXXXXXEPRVIKPSEPNSTKFVPRALRKAAESVGTEGDNDSWRRLDRPIHIIDRRSFFSDDVKQSTSPAHGLSSRDGNPMPRQREADVVPPREKYAPPG-KYEPPTRHTQGKYEAPFSGRRENQYEPPRGRDSQPPIG---QRPKYGGFGIRDNDRDRWGGGPDREKQRDEDRSVGADKWRKP
D5ADZ5_______________KAKLDEMTEKQKQRERELEEKERLRKEALIKEEPRVSRPTEPVPNKFVPRAMRKAAESAGTEADNDRWRRGDRQTHV---------------------DGRDGLPIPRQREADV-PARDKYAPPSRKYEPPTRTTQGKYEPPVSGRRDN-YEAPRGRDAQPPIGRFEQRPKYGGFGNNKDERDRWGAAPDRERQRDEDRSGGADNWRRP

SNPs (tot: 48)

Position	A %	C %	G %	T %	Probability
272	14		85		0.994477
330			85	14	0.994477
368	14	85			0.994477
369	85		14		0.994477
374	14			85	0.994477
375			14	85	0.994477
376		14		85	0.994477
377		14		85	0.994477
384	14	85			0.994477
409	75	25			0.996473
418	25	75			0.996473
423		80	20		0.995493
424	20	80			0.995493
425			16	83	0.994512
426		83		16	0.994512
427		83	16		0.994512
435	12			87	0.992554
437	87		12		0.992554
440		12	87		0.992554
441	12		87		0.992554
443	87		12		0.992554
446			12	87	0.992554
466		85	14		0.993533
471		85	14		0.993533
473		14		85	0.993533
478	85		14		0.993533
481		14	85		0.993533
483	14			85	0.993533
485			14	85	0.993533
498		16		83	0.994512
499		16		83	0.994512
500	16			83	0.994512
636			75	25	0.996473
665			75	25	0.996473
877		25	75		1.0
978		66		33	1.0
1082	25			75	0.996473
1083			25	75	0.996473
1088	25		25		0.995533
1089			75	25	0.996473
1092		33		66	0.997452
1093	33			66	0.997452
1095			66	33	0.997452
1096			33	66	0.997452
1100	33			66	0.997452
1102		66	33		0.997452
1103	33			66	0.997452
1105		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain