SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15953

Length: 2442 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene15953
GCTGCAATTGCAATTTCATTAGGATTCGTCTCTAATTCCAAATGATGTAACAGAAGAGACACGCAACCTCCTTTGTGTTTAACACAAACTATGGCATTAACATTTTTAATTAAAATAATTGCATACCTTTTCCATTGGTTAATGCGTAATGGACTCACATTTTCCCCTGTTANNTAGGTGCGAAGAGAATAGAATGGGGATAGGAACTTTTGCGATTGATTAGCATAGTTTTCTCGAAGTGCAGGGCATAGTCGGTGGAACATATTGTTGCCATTCTGCCTGCTGTTACTAGGGGGCAACTTAACAACTCAGTTGTGATCTTTTTCATTCGAAAAAGAAAATGGTGCTCAGTGCTGCAGTTGAGTCTGAAGACTCAGTCCATTCCTGCATGTTTGCTTCTCGTTATATGCAGTCCTCGTTACCTAAGTTTCGATTGCCAGAAAGATCTATCCCTAAGGATGCTGCCTACCAGATCATTAATGATGAATTGATGTTGGATGGGAATCCCAGATTGAATCTGGCCTCTTTTGTCACTACCTGGATGGAGCCTGAATGTGATAAGCTTATGATGTCTGCTATCAATAAAAATTATGTTGATATGGATGAATACCCTGTCACTACAGATTTACAGAATCGATGTGTGAATATGATAGCTCGGTTGTTCCATGCCGAAGTGGAGGATGGAGATCAGGCTGTTGGCGTTGCAACAGTGGGATCCTCTGAAGCCATCATGCTGGCAGGTCTGGCATTTAAACGCAAATGGCAGAACAAGATGAAGGCACAGGGGAAGCCCTCTGATAAACCCAACATTGTTACTGGTTCCAATGTTCAGGTTTGCTGGGAAAAATTTGCCAGATATTTTGAAGTAGAGTTAAAGGAAGTTAAACTGACGGAAGACTATTATGTAATGAATCCCAAGAAGGCTGTGGAGATGGTGGATGAGAATACCATCTGGTGTGTGCGCCATTCTGGGCTCCACTTACAATGGAGAATTTGAAGATGTTAAGATGCTCAATGACTTACTGTTGCAGAAAAATAAACAAACCGGGTGGGATACTCCTATACATGTGGATGCTGCAAGTGGAGGATTCGTTGCACCTTTTATATATCCTGATTTGGAATGGGACTTCAGTTTACCACTAGTGAGAAGCATCAATGTGAGTGGGCACAAATATGGACTTGTATATGCTGGAGTTGGCTGGGTGATTTGGAGAAGCAAGCAAGACTTGCCTGAAGAACTCATTTTCCACATTAATTATTTAGGAACTGATCAGCCCACATTCACTCTGAATTTCTCCAAAGGGGCAAGTCAGATTATTGGGCAGTACTACCAACTCATTCGCCTTGGTTTCGAGGGATACAAATTGATTATGGAAAATTGCAGAGCGAATGCTAGATATCTGACACAAATCCTGGAAAAGACTGGACGTTTCAAAATTCTGTCGAAAGACATGGGCGTGCCTGTGGTTGCATTCTCGCTCAAGGACAAGAGCCTGGGACACGATGAATACGAGATCTCCGATCATTTGCGGAAGTTCGGGTGGGTTGTTCCCGCTTATACAATGGCTCCCGATGCTCAGCATGTCTTGCTGTTACGCGTTGTTGTTAGGGAGGACTTCAGTAGAAGTCTGGCGGAGCGACTGGCTCAGAATATTGAGCAGGTTTTGCAGGAATTAGAAGCTGCTCATCCTCAAACCGCTACAGTAGTGAAGGAGGTCTTGATAACAGAAGATGGGCAGAATGATCAGGTGGGGGCGAAGGAAAATTCTAAAAAGACTCATGACAAGAGAAGACGGGGACGCCATGCTCGCCATCCTGATTCCGTGGACAAAACAAAGGGCATTTGTTAAATCTTCTTATTTCTATGTTGACCGGTCTTTTCGTTCTGGGTTTACTTACTGAAATAAAGTTTCAAGAAATGTGTAAGCAATTTCCTATAATCATCGGAAAAGAGCGTGTAATTTGTGGCGTGACCGAATAATTATGTGTCGAAAACGGGAAGAAGATCAGAAATTGTGTGACTGCGCAAAATTTCTCCACTGGAGAGGTCCCACCAAGATTTGAACCTGGGTTACTGGTTCAGACAACAATGCCCCTTCACTGGACCTTTGGACCTTTATGTTTGGTTTGTTCCAGAAACGCATTAGCTGGATAATTCGTTAAAAAGATATTCAGGAAAATGGAAAATAGTTGTTGAACTTGTAATATTGAAAATATTACTCTTAATCGTTGCCTACCAGATTAGGTTGAGTCCTTAAAATAAGATCTCGTTTCATATTTAGCGTATATCTGTGGATGTAGATTGCTGTGGAAGTAAGACGAAAATACAAATCCATTTGAAGTCAGTTTTTTTCCGAATTAGGTAGAGTAGGGGATAGGATTAGTTTTAATGTTGGATAGAAAAATGATCTGTTTACAAATAAGAATGTTATCATTAAAAAA

Ace file of the UniGene sp_v1.1_unigene15953

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glutamate decarboxylase n=2 Tax=Pinaceae RepID=A0EJ89_PINPS	-	-	78%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	glutamate decarboxylase	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	Glutamate decarboxylase.	EC:4.1.1.15	-	0.0	87%

Source	KEGGs	Term	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250	87%
Blast2go	beta-Alanine metabolism	00410	87%
Blast2go	Butanoate metabolism	00650	87%
Blast2go	Metabolic pathways	01100	87%
Blast2go	Biosynthesis of secondary metabolites	01110	87%

Source	GOs	Term	Type	Identity
Blast2go	glutamate metabolic process	GO:0006536	Biological Process	87%
Blast2go	pyridoxal phosphate binding	GO:0030170	Molecular Function	87%
Blast2go	glutamate decarboxylase activity	GO:0004351	Molecular Function	87%
Blast2go	calmodulin binding	GO:0005516	Molecular Function	87%
Blast2go	plastid	GO:0009536	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Blast2go	Pyridoxal phosphate-dependent decarboxylase	IPR002129	-	87%
Blast2go	Glutamate decarboxylase	IPR010107	-	87%
Blast2go	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	IPR015421	-	87%
Blast2go	Pyridoxal phosphate-dependent transferase, major domain	IPR015424	-	87%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNM2

Fln msg: Overlapping hits, possible frame ERROR between 961 and 957,

Fln protein: MVLSAAVESEDSVHSCMFASRYMQSSLPKFRLPERSIPKDAAYQIINDELMLDGNPRLNLASFVTTWMEPECDKLMMSAINKNYVDMDEYPVTTDLQNRCVNMIARLFHAEVEDGDQAVGVATVGSSEAIMLAGLAFKRKWQNKMKAQGKPSDKPNIVTGSNVQVCWEKFARYFEVELKEVKLTEDYYVMNPKKAVEMVDENTIWxxxAILGSTYNGEFEDVKMLNDLLLQKNKQTGWDTPIHVDAASGGFVAPFIYPDLEWDFSLPLVRSINVSGHKYGLVYAGVGWVIWRSKQDLPEELIFHINYLGTDQPTFTLNFSKGASQIIGQYYQLIRLGFEGYKLIMENCRANARYLTQILEKTGRFKILSKDMGVPVVAFSLKDKSLGHDEYEISDHLRKFGWVVPAYTMAPDAQHVLLLRVVVREDFSRSLAERLAQNIEQVLQELEAAHPQTATVVKEVLITEDGQNDQVGAKENSKKTHDKRRRGRHARHPDSVDKTKGIC*

Protein Length: 505

Fln nts: GCTGCAATTGCAATTTCATTAGGATTCGTCTCTAATTCCAAATGATGTAACAGAAGAGACACGCAACCTCCTTTGTGTTTAACACAAACTATGGCATTAACATTTTTAATTAAAATAATTGCATACCTTTTCCATTGGTTAATGCGTAATGGACTCACATTTTCCCCTGTTAacTAGGTGCGAAGAGAATAGAATGGGGATAGGAACTTTTGCGATTGATTAGCATAGTTTTCTCGAAGTGCAGGGCATAGTCGGTGGAACATATTGTTGCCATTCTGCCTGCTGTTACTAGGGGGCAACTTAACAACTCAGTTGTGATCTTTTTCATTCGAAAAAGAAA_-_ATGGTGCTCAGTGCTGCAGTTGAGTCTGAAGACTCAGTCCATTCCTGCATGTTTGCTTCTCGTTATATGCAGTCCTCGTTACCTAAGTTTCGATTGCCAGAAAGATCTATCCCTAAGGATGCTGCCTACCAGATCATTAATGATGAATTGATGTTGGATGGGAATCCCAGATTGAATCTGGCCTCTTTTGTCACTACCTGGATGGAGCCTGAATGTGATAAGCTTATGATGTCTGCTATCAATAAAAATTATGTTGATATGGATGAATACCCTGTCACTACAGATTTACAGAATCGATGTGTGAATATGATAGCTCGGTTGTTCCATGCCGAAGTGGAGGATGGAGATCAGGCTGTTGGCGTTGCAACAGTGGGATCCTCTGAAGCCATCATGCTGGCAGGTCTGGCATTTAAACGCAAATGGCAGAACAAGATGAAGGCACAGGGGAAGCCCTCTGATAAACCCAACATTGTTACTGGTTCCAATGTTCAGGTTTGCTGGGAAAAATTTGCCAGATATTTTGAAGTAGAGTTAAAGGAAGTTAAACTGACGGAAGACTATTATGTAATGAATCCCAAGAAGGCTGTGGAGATGGTGGATGAGAATACCATCTGGxxxxxxxxxGCCATTCTGGGCTCCACTTACAATGGAGAATTTGAAGATGTTAAGATGCTCAATGACTTACTGTTGCAGAAAAATAAACAAACCGGGTGGGATACTCCTATACATGTGGATGCTGCAAGTGGAGGATTCGTTGCACCTTTTATATATCCTGATTTGGAATGGGACTTCAGTTTACCACTAGTGAGAAGCATCAATGTGAGTGGGCACAAATATGGACTTGTATATGCTGGAGTTGGCTGGGTGATTTGGAGAAGCAAGCAAGACTTGCCTGAAGAACTCATTTTCCACATTAATTATTTAGGAACTGATCAGCCCACATTCACTCTGAATTTCTCCAAAGGGGCAAGTCAGATTATTGGGCAGTACTACCAACTCATTCGCCTTGGTTTCGAGGGATACAAATTGATTATGGAAAATTGCAGAGCGAATGCTAGATATCTGACACAAATCCTGGAAAAGACTGGACGTTTCAAAATTCTGTCGAAAGACATGGGCGTGCCTGTGGTTGCATTCTCGCTCAAGGACAAGAGCCTGGGACACGATGAATACGAGATCTCCGATCATTTGCGGAAGTTCGGGTGGGTTGTTCCCGCTTATACAATGGCTCCCGATGCTCAGCATGTCTTGCTGTTACGCGTTGTTGTTAGGGAGGACTTCAGTAGAAGTCTGGCGGAGCGACTGGCTCAGAATATTGAGCAGGTTTTGCAGGAATTAGAAGCTGCTCATCCTCAAACCGCTACAGTAGTGAAGGAGGTCTTGATAACAGAAGATGGGCAGAATGATCAGGTGGGGGCGAAGGAAAATTCTAAAAAGACTCATGACAAGAGAAGACGGGGACGCCATGCTCGCCATCCTGATTCCGTGGACAAAACAAAGGGCATTTGTTAA___ATCTTCTTATTTCTATGTTGACCGGTCTTTTCGTTCTGGGTTTACTTACTGAAATAAAGTTTCAAGAAATGTGTAAGCAATTTCCTATAATCATCGGAAAAGAGCGTGTAATTTGTGGCGTGACCGAATAATTATGTGTCGAAAACGGGAAGAAGATCAGAAATTGTGTGACTGCGCAAAATTTCTCCACTGGAGAGGTCCCACCAAGATTTGAACCTGGGTTACTGGTTCAGACAACAATGCCCCTTCACTGGACCTTTGGACCTTTATGTTTGGTTTGTTCCAGAAACGCATTAGCTGGATAATTCGTTAAAAAGATATTCAGGAAAATGGAAAATAGTTGTTGAACTTGTAATATTGAAAATATTACTCTTAATCGTTGCCTACCAGATTAGGTTGAGTCCTTAAAATAAGATCTCGTTTCATATTTAGCGTATATCTGTGGATGTAGATTGCTGTGGAAGTAAGACGAAAATACAAATCCATTTGAAGTCAGTTTTTTTCCGAATTAGGTAGAGTAGGGGATAGGATTAGTTTTAATGTTGGATAGAAAAATGATCTGTTTACAAATAAGAATGTTATCATTAAAAAA

Fln Alignment:

sp_v1.1_unigene15953___MVLSAAVESEDSVHSCMFASRYMQSSLPKFRLPERSIPKDAAYQIINDELMLDGNPRLNLASFVTTWMEPECDKLMMSAINKNYVDMDEYPVTTDLQNRCVNMIARLFHAEVEDGDQAVGVATVGSSEAIMLAGLAFKRKWQNKMKAQGKPSDKPNIVTGSNVQVCWEKFARYFEVELKEVKLTEDYYVMNPKKAVEMVDENTIWCxCAILGSTYNGEFEDVKMLNDLLLQKNKQTGWDTPIHVDAASGGFVAPFIYPDLEWDFSLPLVRSINVSGHKYGLVYAGVGWVIWRSKQDLPEELIFHINYLGTDQPTFTLNFSKGASQIIGQYYQLIRLGFEGYKLIMENCRANARYLTQILEKTGRFKILSKDMGVPVVAFSLKDKSLGHDEYEISDHLRKFGWVVPAYTMAPDAQHVLLLRVVVREDFSRSLAERLAQNIEQVLQELEAAHPQTATVVKEVLITEDGQNDQVGAKENSKKTHDKRRRGRHARHPDSVDKTKGIC
B8LNM2_______________MVLTTATESNDSINSCMFASRYMQTPLPRFRMPEKSIPKDAAYQIINDELMLDGNPRLNLASFVTTWMEPECDKLMMSAVNKNYVDMDEYPVTTDLQNRCVNMIARLFHAEVEDGDQAVGCATVGSSEAIMLAGLAFKRKWQNKRKAQGKPYDKPNIVTGSNVQVCWEKFARYFEVELKEVKLREGYYVMDPKKAAAMVDENTI-CxCAILGSTYNGEFEDVKMLNDLLVQKNKETGWNTPIHVDAASGGFIAPFIYPDLEWDFRLPLVRSINVSGHKYGLVYAGVGWVVWRSKQDLPEELIFHINYLGADQPTFTLNFSKGASQIIGQYYQLIRLGFEGYKLIMENCRANARYLTEILEKTGRFSILSKDVGVPVVAFSLKDKSLGHDEYEISDHLRKFGWVVPAYTMAPDAQHVLLLRVVVREDFSRTLAERLAHNIEQVLQEFEALHPKINKVVEEILITEDDKNDQVEANENSKKTHEKRRTGRHARHPDSVAKTKGIC

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
2004			83	16	1.0
2077		80		20	1.0
2162		42		42	1.0
2164	80			20	0.995493
2278		10		90	0.90298
2287			12	87	0.992554
2346		50		50	1.0
2374			50	50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain