SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15812

Length: 2522 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene15812
TGCTGTCAATTCAGACCACATGAAAAGTCGCGAGTCCTTAAGCTAGTGTCCTATCGAATCACAAACCAGACAAAGACGATGACCCCAATTTGTGTTTATCTTAATAAATTTAATGTTATCAGTCGCAATGGTTGGATCCAATTACCCTAATATCTAGAGTTGCCTTTGTTTGGTAGGAGCCATTACCCTCAGATCTAGGGTGGCCTTGATCTGGGACACCACGCTTGTGTTCGCTGAAAGTGTGCCACCAAACTGGTTAGATGAAAAGTCAAAATTCCCCTAGCTGAGAAAGAAGGCACATATTTCTCAGTCGCTTACATTATTATAAAATCTTAGAACTCCAAAGGTTTCCCAAATACTTCCTCTACTGCTGCTCGTAAATATTTGCACCTTGAGAGGCTCCGGTGTACAAGAGGAAGAAGAGGAACGGGAGCCATATTACAGTAACAATGCAGGGAGCCAACTTATTGCTGGAGGAGCCCATCCGCATGGCATCCATTCTCGAGCCTTCCAAGGCTAATTTCTTTCCTGCAATGACAAAAATTATTGGTACTCTTGGTCCAAAGTCACGATCTGTTGAGGAGATTGAGGGGTGCCTTAAGGCTGGGATGTCTGTTGCTCGTTTTGATTTTTCTGGGGGAGACAATGACTATCACCAAGAAACTCTTGAAAATCTGAAGATTGCTGTGAAAGGAACTAAAAAACTTTGTGCTATCATGCTGGATACAGTTGGTCCAGAGTTGCAAGTTTTTAACAAGAGTGAGGAACCTATTGTGCTTGAGATTGATGCATTCGTGACTCTGACACCAGATCAGACCAAAGAAGCATCATCCGAAGTCTTGCCAATAAATTATGCTGGGCTAGCCAAGACTGTGACAAAAGGGGATACGATATTTGTTGGTCAATATCTCTTTACAGGAAGTGAGACCACTTCTGTCTGGCTTGAGGTTGCTGCAATTAAAGATCAAGATGTAGTTTGTTGTGTCAAAAACAGTGCAACTCTTGCTGGTTCATTGTTTACAATGCACATCTCTCAAGTTCATATTGATCTTCCAACACTAAGTGATACAGATAAGCATGTTATTGCAACTTGGGGAGTTCGCAACAACATTGACTTTCTTTCTTTGTCATACACACGACATGCTGAAGACATTAGACAAACCCGTGCTTATCTTGATGAGCTTGGGAATCTTCGACAGACACAAATATTTGCTAAGATTGAGACTATAGAGGGTCTGCAGCATTTTGATGAGATCTTGCAAGAGGCAGATGGTATTATTCTTTCACGGGGTAATTTAGGAATAGATCTTCAACCGGAGAAGGTGTTTTTATTCCAGAAAGCTGCTGTCCACAAATGTAACATGGCTGGAAAGCCTGCTGTAGTGACACGTGTTGTTGACAGTATGTCGGGTAATTTACGTCCAACACGTGCAGAAGCAACTGATGTCGCAAATGCTGTTCTGGATGGGACAGATGCAATTCTGTTAGGTGCAGAAACTTTCCGCGGATTATACCCCTTTGGGACTATCTCAATTGTAGGAAAGATCTGTGCAGAAGCAGAGAAAGTGTACAATCAAGCTGTTTATTTTAAGAGGACTATGAAACTTAGCATTGAACCAATGAGTCACTTGGAATCTATTGCAACCACGGCTGTAAGTGCAGCAATCAATGTGAAGGCTTCAGTTATTGTTGTGTTCACAGCATCTGGACGAACTGCTAGGTTAATAGCAAAATATCGTCCTACTATGCCAGTGTTATCTGTTGTCGTCCCAAGATTGACAACAAATCAGTTGAGATGGAGCTTTACTGGTGCATTTCAAGCAAGACAGACTCTTGTTGTTAGAGGACTTTTTCCTATGCTGGCAGATCCTCGACATCCAGCTGAATCAATTAATGCAACAAATGAGTCTGTATTGAAGATTGCATTGGATCATGGTAAAACAGTAGGGTTAATTAAGCCTCATGATCGAATTGTTGTATGCCAAAAGATAGGGGACTCGGCTGTTGTTAAGATACTTGAGCTTGAAGATTAGGACGATACAGAAGGCAGTTGGCAGATTTTAGACTTGTGTACAACATTTCCCTAGGTATATGCCAAATGTGCATAGTTATGCATGAACAGTGAATTTTGAAGAGGTTTTAATTTGCATTTAAAACTCATCAGCAAAGTTTATCTTTATGAGATCAACCAATAATTAGCCAAATTCAGCATGGTACACAACTTCGCAAGTTTAATGAAACAAGTGCTGTATTAGATTTTTTTCTAATACACATGATTTGATTTCATATGGATTTTATTCAGGAGCCTGTAATAGTGAAATCTTGAAATAATATTTACCTAGATTAATTTGTTTTAGATGAAGACAGATGAAATCCTGGACATATCAAGTTAGATCCATAATTTATGAAGTATTGGTTGTGAACCAAAATACAAGAATAAATGGTATAATTTTGTGAAAAATGAACAATTATGGTGTAATCCGAGAAATTGAAATTGACCGGTTAATAGATTGGGTTTTCAT

Ace file of the UniGene sp_v1.1_unigene15812

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pyruvate kinase n=1 Tax=Picea sitchensis RepID=A9NUL1_PICSI	-	-	95%
FL-Next	sp=Pyruvate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	pyruvate kinase	-	-	88%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:2.7.1.4-	-	0.0	88%

Source	GOs	Term	Type	Identity
Blast2go	response to cadmium ion	GO:0046686	Biological Process	88%
Blast2go	glycolysis	GO:0006096	Biological Process	88%
Blast2go	pyruvate kinase activity	GO:0004743	Molecular Function	88%
Blast2go	potassium ion binding	GO:0030955	Molecular Function	88%
Blast2go	magnesium ion binding	GO:0000287	Molecular Function	88%
Blast2go	plasma membrane	GO:0005886	Cellular Component	88%

Source	InterPros	Term	Type	Identity
Blast2go	Pyruvate kinase	IPR001697	-	88%
Blast2go	Pyruvate kinase-like, insert domain	IPR011037	-	88%
Blast2go	Pyruvate kinase, barrel	IPR015793	-	88%
Blast2go	Pyruvate kinase, alpha/beta	IPR015794	-	88%
Blast2go	Pyruvate kinase, C-terminal	IPR015795	-	88%
Blast2go	Pyruvate kinase, beta-barrel insert domain	IPR015806	-	88%
Blast2go	Pyruvate/Phosphoenolpyruvate kinase	IPR015813	-	88%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUL1

Fln msg:

Fln protein: MQGANLLLEEPIRMASILEPSKANFFPAMTKIIGTLGPKSRSVEEIEGCLKAGMSVARFDFSGGDNDYHQETLENLKIAVKGTKKLCAIMLDTVGPELQVFNKSEEPIVLEIDAFVTLTPDQTKEASSEVLPINYAGLAKTVTKGDTIFVGQYLFTGSETTSVWLEVAAIKDQDVVCCVKNSATLAGSLFTMHISQVHIDLPTLSDTDKHVIATWGVRNNIDFLSLSYTRHAEDIRQTRAYLDELGNLRQTQIFAKIETIEGLQHFDEILQEADGIILSRGNLGIDLQPEKVFLFQKAAVHKCNMAGKPAVVTRVVDSMSGNLRPTRAEATDVANAVLDGTDAILLGAETFRGLYPFGTISIVGKICAEAEKVYNQAVYFKRTMKLSIEPMSHLESIATTAVSAAINVKASVIVVFTASGRTARLIAKYRPTMPVLSVVVPRLTTNQLRWSFTGAFQARQTLVVRGLFPMLADPRHPAESINATNESVLKIALDHGKTVGLIKPHDRIVVCQKIGDSAVVKILELED*

Protein Length: 529

Fln nts: TGCTGTCAATTCAGACCACATGAAAAGTCGCGAGTCCTTAAGCTAGTGTCCTATCGAATCACAAACCAGACAAAGACGATGACCCCAATTTGTGTTTATCTTAATAAATTTAATGTTATCAGTCGCAATGGTTGGATCCAATTACCCTAATATCTAGAGTTGCCTTTGTTTGGTAGGAGCCATTACCCTCAGATCTAGGGTGGCCTTGATCTGGGACACCACGCTTGTGTTCGCTGAAAGTGTGCCACCAAACTGGTTAGATGAAAAGTCAAAATTCCCCTAGCTGAGAAAGAAGGCACATATTTCTCAGTCGCTTACATTATTATAAAATCTTAGAACTCCAAAGGTTTCCCAAATACTTCCTCTACTGCTGCTCGTAAATATTTGCACCTTGAGAGGCTCCGGTGTACAAGAGGAAGAAGAGGAACGGGAGCCATATTACAGTAACA_-_ATGCAGGGAGCCAACTTATTGCTGGAGGAGCCCATCCGCATGGCATCCATTCTCGAGCCTTCCAAGGCTAATTTCTTTCCTGCAATGACAAAAATTATTGGTACTCTTGGTCCAAAGTCACGATCTGTTGAGGAGATTGAGGGGTGCCTTAAGGCTGGGATGTCTGTTGCTCGTTTTGATTTTTCTGGGGGAGACAATGACTATCACCAAGAAACTCTTGAAAATCTGAAGATTGCTGTGAAAGGAACTAAAAAACTTTGTGCTATCATGCTGGATACAGTTGGTCCAGAGTTGCAAGTTTTTAACAAGAGTGAGGAACCTATTGTGCTTGAGATTGATGCATTCGTGACTCTGACACCAGATCAGACCAAAGAAGCATCATCCGAAGTCTTGCCAATAAATTATGCTGGGCTAGCCAAGACTGTGACAAAAGGGGATACGATATTTGTTGGTCAATATCTCTTTACAGGAAGTGAGACCACTTCTGTCTGGCTTGAGGTTGCTGCAATTAAAGATCAAGATGTAGTTTGTTGTGTCAAAAACAGTGCAACTCTTGCTGGTTCATTGTTTACAATGCACATCTCTCAAGTTCATATTGATCTTCCAACACTAAGTGATACAGATAAGCATGTTATTGCAACTTGGGGAGTTCGCAACAACATTGACTTTCTTTCTTTGTCATACACACGACATGCTGAAGACATTAGACAAACCCGTGCTTATCTTGATGAGCTTGGGAATCTTCGACAGACACAAATATTTGCTAAGATTGAGACTATAGAGGGTCTGCAGCATTTTGATGAGATCTTGCAAGAGGCAGATGGTATTATTCTTTCACGGGGTAATTTAGGAATAGATCTTCAACCGGAGAAGGTGTTTTTATTCCAGAAAGCTGCTGTCCACAAATGTAACATGGCTGGAAAGCCTGCTGTAGTGACACGTGTTGTTGACAGTATGTCGGGTAATTTACGTCCAACACGTGCAGAAGCAACTGATGTCGCAAATGCTGTTCTGGATGGGACAGATGCAATTCTGTTAGGTGCAGAAACTTTCCGCGGATTATACCCCTTTGGGACTATCTCAATTGTAGGAAAGATCTGTGCAGAAGCAGAGAAAGTGTACAATCAAGCTGTTTATTTTAAGAGGACTATGAAACTTAGCATTGAACCAATGAGTCACTTGGAATCTATTGCAACCACGGCTGTAAGTGCAGCAATCAATGTGAAGGCTTCAGTTATTGTTGTGTTCACAGCATCTGGACGAACTGCTAGGTTAATAGCAAAATATCGTCCTACTATGCCAGTGTTATCTGTTGTCGTCCCAAGATTGACAACAAATCAGTTGAGATGGAGCTTTACTGGTGCATTTCAAGCAAGACAGACTCTTGTTGTTAGAGGACTTTTTCCTATGCTGGCAGATCCTCGACATCCAGCTGAATCAATTAATGCAACAAATGAGTCTGTATTGAAGATTGCATTGGATCATGGTAAAACAGTAGGGTTAATTAAGCCTCATGATCGAATTGTTGTATGCCAAAAGATAGGGGACTCGGCTGTTGTTAAGATACTTGAGCTTGAAGATTAG___GACGATACAGAAGGCAGTTGGCAGATTTTAGACTTGTGTACAACATTTCCCTAGGTATATGCCAAATGTGCATAGTTATGCATGAACAGTGAATTTTGAAGAGGTTTTAATTTGCATTTAAAACTCATCAGCAAAGTTTATCTTTATGAGATCAACCAATAATTAGCCAAATTCAGCATGGTACACAACTTCGCAAGTTTAATGAAACAAGTGCTGTATTAGATTTTTTTCTAATACACATGATTTGATTTCATATGGATTTTATTCAGGAGCCTGTAATAGTGAAATCTTGAAATAATATTTACCTAGATTAATTTGTTTTAGATGAAGACAGATGAAATCCTGGACATATCAAGTTAGATCCATAATTTATGAAGTATTGGTTGTGAACCAAAATACAAGAATAAATGGTATAATTTTGTGAAAAATGAACAATTATGGTGTAATCCGAGAAATTGAAATTGACCGGTTAATAGATTGGGTTTTCAT

Fln Alignment:

sp_v1.1_unigene15812___MQGANLLLEEPIRMASILEPSKANFFPAMTKIIGTLGPKSRSVEEIEGCLKAGMSVARFDFSGGDNDYHQETLENLKIAVKGTKKLCAIMLDTVGPELQVFNKSEEPIVLEIDAFVTLTPDQTKEASSEVLPINYAGLAKTVTKGDTIFVGQYLFTGSETTSVWLEVAAIKDQDVVCCVKNSATLAGSLFTMHISQVHIDLPTLSDTDKHVIATWGVRNNIDFLSLSYTRHAEDIRQTRAYLDELGNLRQTQIFAKIETIEGLQHFDEILQEADGIILSRGNLGIDLQPEKVFLFQKAAVHKCNMAGKPAVVTRVVDSMSGNLRPTRAEATDVANAVLDGTDAILLGAETFRGLYPFGTISIVGKICAEAEKVYNQAVYFKRTMKLSIEPMSHLESIATTAVSAAINVKASVIVVFTASGRTARLIAKYRPTMPVLSVVVPRLTTNQLRWSFTGAFQARQTLVVRGLFPMLADPRHPAESINATNESVLKIALDHGKTVGLIKPHDRIVVCQKIGDSAVVKILELED
A9NUL1_______________MQGAHLLLEEPIRMASILEPSKANFFHAMTKIVGTLGPNSRSVEVIEGCLKAGMSVARFDFSGGDNDYHQETLENLKIAVKEIKKLCAIMLDTVGPELQVFNRSEEPIVLEIDAFVTLTPDQSKEASSEVLPINYAGLAKTVTKGDTIFVGQYLFTGSETTSVWLEVAEIKDQDVVCCVKNSATLAGALFTMHISQVHIDLPTLSDTDKHVIATWGVRNNIDFLSLSYTRHAEDIRQTRAYLDELGNLQQTQIFAKIETIEGLEHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAAVHKCNMAGKPAVVTRVVDSMSGNLRPTRAEATDVANAVLDGTDAILLGAETFRGLYPFETISIVGKICAEAEKVYNQAVYFKRTMKHSNEPMSHLESIATTAVSAAINVKASVIVVFTASGRTARLIAKYRPTMPVLSVVIPRLTTNQLRWSFTGAFQARQTLIVRGVFPMLADPRHSAESINATNESVLEIALDHGKTAGLIKPHDRIVVCQKVGDSAVVKILELED

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
1847	66		33		0.997452
1885		87	12		0.992554
1886			12	87	0.992554
1893	80		20		0.999996
2052	93			6	0.999264
2055		6		93	0.999264
2056	6		93		0.999264
2057		6	93		0.999264
2374	93		6		0.99903
2458			30	69	1.0
2492	18			81	0.999994
2493	18			81	0.999994
2495	90	10			0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain