SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15744

Length: 1099 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene15744

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Elongation factor 2 n=16 Tax=Magnoliophyta RepID=EF2_BETVU	-	-	88%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	protein	-	-	91%

Source	ECs	Term	Type	Identity
Blast2go	Protein-synthesizing GTPase.	EC:3.6.5.3	-	91%
Blast2go	Heterotrimeric G-protein GTPase.	EC:3.6.5.1	-	91%
Blast2go	Small monomeric GTPase.	EC:3.6.5.2	-	91%
Blast2go	Signal-recognition-particle GTPase.	EC:3.6.5.4	-	91%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	91%
Blast2go	translation	GO:0006412	Biological Process	91%
Blast2go	translation elongation factor activity	GO:0003746	Molecular Function	91%
Blast2go	GTP binding	GO:0005525	Molecular Function	91%
Blast2go	copper ion binding	GO:0005507	Molecular Function	91%
Blast2go	GTPase activity	GO:0003924	Molecular Function	91%
Blast2go	plasma membrane	GO:0005886	Cellular Component	91%
Blast2go	chloroplast	GO:0009507	Cellular Component	91%
Blast2go	cytosol	GO:0005829	Cellular Component	91%

Source	InterPros	Term	Type	Identity
Blast2go	Translation elongation factor EFG/EF2, C-terminal	IPR000640	-	91%
Blast2go	Translation elongation factor EFG/EF2, domain IV	IPR005517	-	91%
Blast2go	Elongation factor G/III/V	IPR009022	-	91%
Blast2go	Ribosomal protein S5 domain 2-type fold, subgroup	IPR014721	-	91%
Blast2go	Ribosomal protein S5 domain 2-type fold	IPR020568	-	91%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACX5

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 250 - 267

Fln protein: KKVLPEAIDDGRIGPKDDPKARAKILSEEFGWDKDLAKKIWCFGPETTGPNMVVDMCKGVQYLNEIKDSVVAAFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQVIPTARRVMYASQLTAKPRLLEPVYLVEIQAPENALGGIYGVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSTLRAATSGQAFPQCVFDHWDMMGSDPLEPGSQAGQLVTDIRKRKGLKESMTPLSEFEDKL*

Protein Length: 251

Fln nts: TGAATGATCAAAAGGATAAGTTGTGGCCCATGTTGCAGAAATTGGGAGTTGTAATGAAAAGTGACGAGAAGGAGCTGGTTGCAAAAGCACTCATGAAGCGTGTCATGCAAACCTGGCTACCTGCAAGTAGTGCTTTACTTGAAGCAAGACCTTTAGAAGAAGGTCTTGCCAGAAGCAATTGATGATGGTCGCATTGGCCCAAAGGATGATCCAAAGGCTCGCGCAAAAATCCTTTCTGAGGAATTTGGATGGGACAAAGATTTGGCTAAGAAGATCTGGTGTTTTGGACCTGAAACCACTGGTCCCAATATGGTGGTGGATATGTGTAAGGGAGTTCAATACTTGAATGAAATTAAAGATTCTGTGGTTGCTGCATTCCAATGGGCATCTAAGGAAGGTGCTCTTGCAGAAGAAAATATGCGTGGTATTTGCTTTGAGGTTTGTGACGTGGTTTTGCACACTGATGCTATCCATAGAGGAGGTGGCCAGGTTATCCCAACGGCCAGAAGGGTTATGTATGCATCTCAGCTTACTGCCAAACCACGGCTTCTTGAGCCAGTATATCTCGTTGAGATTCAAGCTCCTGAGAATGCCCTTGGTGGTATTTACGGTGTGCTGAACCAGAAGAGAGGTCATGTTTTTGAAGAAATGCAGAGGCCAGGTACTCCACTATACAACATCAAGGCATATCTGCCAGTTGTTGAATCATTCGGATTCTCAAGCACTCTCCGTGCTGCAACTTCTGGCCAGGCTTTCCCTCAATGTGTGTTTGACCACTGGGATATGATGGGCTCTGATCCATTGGAACCTGGTTCCCAAGCTGGGCAGCTTGTGACTGATATCCGTAAGAGGAAGGGTCTTAAGGAGAGTATGACTCCCTTGTCAGAGTTCGAAGACAAGCTGTAG___AGCTTTGCTATGTTTGCATGTCGGATGCTGTCAAGATTGAGGAACCTCCGAGTATTAAAACACAGTTTTGTGTGCTAGGACTAGTTGAATTTATGCTATTCAGGTATTTTTGTGATCTGTTATTTATGTTATTCAGGTATTTTTGTGATTTGAAATTACTTTTTACAAGGTACTTCGCAGTGAGTGACAGGCC

Fln Alignment:

sp_v1.1_unigene15744___LYLKQDL*KKVLPEAIDDGRIGPKDDPKARAKILSEEFGWDKDLAKKIWCFGPETTGPNMVVDMCKGVQYLNEIKDSVVAAFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQVIPTARRVMYASQLTAKPRLLEPVYLVEIQAPENALGGIYGVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSTLRAATSGQAFPQCVFDHWDMMGSDPLEPGSQAGQLVTDIRKRKGLKESMTPLSEFEDKL
D5ACX5_______________LYFEARPLEEGLAEAIDDGRIGPKDDPKARAKILSEEFGWDKDLAKKIWCFGPETTGPNLVVDMCKGVQYLNEIKDSVVAAFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQIIPTARRVMYASQLTAKPRLLEPVYLVEIQAPENALGGIYGVLNQKRGHVFEEMQRQGTPLYNIKAYLPVVESFGFSSTLRAATSGQAFPQCVFDHWDMMGSDPLETGSQAGQLVTDIRKRKGLKESMTPLSDFEDKL

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
685	83	16			0.994512
686	83			16	0.994512
787	93		6		0.999999
983	10		90		0.90298
1012			90	10	0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain