SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15735

Length: 1533 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene15735 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Palmitoyl-protein thioesterase 1 precursor, putative (EC:3.1.2.22); K01074 palmitoyl-protein thioesterase [EC:3.1.2.22]	-	-	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	86%
Blast2go	palmitoyl-protein thioesterase 1	-	-	81%

Source	ECs	Term	Type	e value	Identity
Blast2go	Palmitoyl-protein hydrolase.	EC:3.1.2.22	-	0.0	81%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Fatty acid elongation in mitochondria	00062		0.0	81%
Blast2go	Metabolic pathways	01100		0.0	81%

Source	ECs	Term	Type	e value	Identity
AutoFact	Palmitoyl-protein hydrolase.	EC:3.1.2.22	-	0.0	69%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Fatty acid elongation in mitochondria	00062		0.0	69%
AutoFact	Metabolic pathways	01100		0.0	69%

Source	GOs	Term	Type	Identity
Blast2go	protein modification process	GO:0006464	Biological Process	81%
Blast2go	palmitoyl-(protein) hydrolase activity	GO:0008474	Molecular Function	81%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	81%
Blast2go	vacuole	GO:0005773	Cellular Component	81%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Palmitoyl protein thioesterase	IPR002472	-	0.0	81%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NRL7

Fln msg: Unexpected STOP codon in 5 prime region,

Fln protein: PWLLDCCYCSVLWHFFHLRFHSPSSFCMALVTSAKNRGVNHFTELLSNWSGSSGSCIEIGDGSMTSWTMPLEKQAECVCEKVKAMPELKNGYNMVGLSQGNVIGRAVIEFCDGGPPVYNMISLGGPHAGTASVPLCGTSFICALVDALIKGELYSDFVQEHLAPSGYLKIPTDISEYLKGCKFLPKLNNEKPDERNSTYKERFSSLKNLVLIMFEHDTVLIPRETAWFGYYPDGNFSVILPVNETALYKEDWIGLKTLDDAGKVKYFSLPGNHLGIRPSDMLKYVVPYLEEDAVIREMAQQPVLNS*

Protein Length: 308

Fln nts: CGTCGTGACCTTGGCTGTCAGTGGATACGCATTTCCCACTCTCCACGAAACTCGAGATTCTTACAGGTCCCCACTACCATTCCGGTTCGGCCAGACGAACAAAAGACTACGTCGTGACCTTGGCTGTCACTCAATTTATTCCCCATCCACGATTGGTGTGGAGATTGTTCAATAATCCCAAAACTGCAAAATCCTAACGGATTCGATCGTGAACCGGAGCTGGAGGCAGATGACCATGGCTTCTCGATTGTTGCTACTGCTCTGTACTCTGGCACTTCTTCCACTTGCGTTTTCACTCCCCTTCATCGTTTTGCATGGCATTGGTGACAAGTGCAAAAAACAGAGGTGTTAACCACTTCACTGAGCTACTGAGCAATTGGTCTGGATCTTCAGGATCATGCATAGAAATTGGAGACGGTTCAATGACATCCTGGACTATGCCTCTAGAGAAGCAGGCTGAATGTGTGTGTGAGAAGGTGAAAGCCATGCCCGAGTTGAAGAATGGGTACAACATGGTTGGTCTATCCCAGGGCAATGTGATAGGTCGTGCAGTCATTGAATTTTGTGATGGAGGGCCTCCTGTTTACAACATGATATCATTAGGGGGGCCACATGCTGGAACTGCTTCTGTACCTCTTTGTGGGACATCATTTATCTGTGCGCTTGTCGATGCTTTAATTAAGGGCGAACTATATTCTGATTTTGTGCAGGAACATCTGGCTCCGAGTGGTTATCTAAAGATTCCGACGGATATATCTGAATATTTAAAAGGTTGTAAGTTTCTCCCAAAGCTTAACAATGAAAAGCCAGATGAGCGAAATTCCACATACAAGGAGCGCTTCAGTAGCCTTAAAAACTTGGTTCTAATCATGTTTGAGCACGATACTGTTTTGATTCCACGCGAGACAGCATGGTTTGGATATTATCCTGATGGAAATTTCAGTGTGATATTGCCTGTAAACGAGACTGCTCTGTATAAGGAAGATTGGATTGGCTTGAAGACCTTGGATGATGCCGGAAAAGTGAAATACTTCAGTTTACCTGGGAATCACCTTGGTATCCGTCCTAGTGACATGCTCAAGTACGTTGTTCCCTACTTAGAAGAAGATGCAGTTATCCGTGAAATGGCTCAACAGCCAGTCCTGAATTCATAA___AATGTTTACTGGAGAGAAGGCCATACAATTCGTTAGTAGTTTTTCTTGCAATTTATGAAAGAATCATGTAGTAAATTGTCAATTGTGAAAGCTTCCCTGTAATATTACAGCATAGTCTTTTGGATGGCTGACTACATCAAAACTTAAATGGGATCAAGAATATCTGTTATGTATAAAACATCAAAGATTGTTTATCGCGGGTTATTATAATGAATTGTATGCCTCAACCTCACTTTTACTCGAACAAGGGTGAAAGAGATCCATCTCTTCATATTGCTCCATTCTTACGTGATTTTCCAATATAAAATGTCTAGTACATGGATTTTGTAATAGAGTTATTCGGTTAAAATTTTAATGTAAATTGTACTTAAATATGTTT

Fln Alignment:

sp_v1.1_unigene15735___LRFHSPSSFCMALVTSAKNRGVNHFTELLSNWSGSSGSCIEIGDGSMTSWTMPLEKQAECVCEKVKAMPELKNGYNMVGLSQGNVIGRAVIEFCDGGPPVYNMISLGGPHAGTASVPLCGTSFICALVDALIKGELYSDFVQEHLAPSGYLKIPTDISEYLKGCKFLPKLNNEKPDERNSTYKERFSSLKNLVLIMFEHDTVLIPRETAWFGYYPDGNFSVILPVNETALYKEDWIGLKTLDDAGKVKYFSLPGNHLGIRPSDMLKYVVPYLEEDAVIREMAQQPVLNS
A9NRL7_______________LAFSLPFIVLHGIGDKCKNKGVSHFTELLSNWSGSSGSCIEIGDGYMTSWTMPLEKQAEIVCEQVKAMPDLEDGYNIVGLSQGNVIGRGIIEFCDGGPPVYNMISLGGPHAGTASVPLCGTSFICELVDALIKGELYSDFVQEHLAPSGYLKIPTDISKYLKGCRFLPKLNNELPDERNSTYKERFSSLKNLILIMFEHDTVLIPRETAWFGYYPDGNFSVILPANETALYKEDWIGLKTLDDAGRVKYISVPGNHLGITPSDMLKHVVPYLEEDAVIREMAQQLVPNA

SNPs (tot: 80)

Position	A %	C %	G %	T %	Probability
353	9			90	0.999872
362	9			90	0.999872
364		90	9		0.999872
513			16	83	0.999991
515		16		83	0.999991
516	16	83			0.999991
542	88		3	7	0.998862
543	92		7		0.999602
544	7		3	88	0.998862
545		88	7	3	0.998862
546		88	3	7	0.998862
547	92		7		0.999602
551	11			88	1.0
552			11	88	1.0
555	10			90	1.0
556	10			90	1.0
557	83		10		1.0
558			10	90	1.0
559	83		6	10	1.0
563	93			6	0.999364
565	7	92			0.999602
568		7		92	0.999602
570	7			92	0.999602
571	7	92			0.999664
573			7	92	0.999664
575		7		92	0.999664
579		7	92		0.999664
615		90	9		0.999872
624			84	15	1.0
625	16	61	16		1.0
626	16			61	1.0
629		66	33		1.0
630	38			61	1.0
632	33		5		1.0
635		33	66		1.0
636	35			64	1.0
637	35		64		1.0
639	64		35		1.0
644	62	37			1.0
645	62		37		1.0
648	62		37		1.0
767		25		75	0.996473
768		25		75	0.996473
769	75	25			0.996473
771	75			25	0.996473
774		25		75	0.996473
792	66			33	0.997452
1231			88	11	0.923564
1232	88		11		0.923564
1235			88	11	0.923564
1239		11	88		0.923564
1240	11			88	0.923564
1242	88	11			0.923564
1243	88	11			0.923564
1246		88	11		0.923564
1348	22		72		1.0
1356	13		86		1.0
1369	86	4		9	0.999642
1370	86		9	4	0.999642
1371	13		4	81	1.0
1372	4	81	9		0.999363
1374	4	81	9	4	0.999363
1375		86	4		0.999642
1383			13	86	1.0
1384	4	80			1.0
1387	5	15	80		1.0
1389	78		21		1.0
1390		15		84	1.0
1391		10	89		0.999919
1392	16	11	72		1.0
1393		29	70		1.0
1394		11	88		0.999952
1402	33			66	1.0
1403	33		66		1.0
1408	75	25			1.0
1413	25	75			1.0
1416	25		75		1.0
1417		25	75		1.0
1423	75			25	1.0
1425	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain