SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15677

Length: 1876 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene15677 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	cysteine desulfurylase, putative (EC:2.8.1.7); K04487 cysteine desulfurase [EC:2.8.1.7]	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	protein	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Cysteine desulfurase.	EC:2.8.1.7	-	0.0	89%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Thiamine metabolism	00730		0.0	89%
Blast2go	Transferases, Transferring nitrogenous groups, Transaminases (aminotransferases).	EC:2.6.1.-	-	0.0	89%

Source	KEGGs	Term	Identity
Blast2go	Cysteine and methionine metabolism	00270	89%
Blast2go	Lysine biosynthesis	00300	89%
Blast2go	Lysine degradation	00310	89%
Blast2go	Arginine and proline metabolism	00330	89%
Blast2go	Phenylalanine metabolism	00360	89%
Blast2go	Tryptophan metabolism	00380	89%
Blast2go	Phosphonate and phosphinate metabolism	00440	89%
Blast2go	Selenocompound metabolism	00450	89%
Blast2go	Glucosinolate biosynthesis	00966	89%
Blast2go	Metabolic pathways	01100	89%
Blast2go	Biosynthesis of secondary metabolites	01110	89%

Source	ECs	Term	Type	e value	Identity
AutoFact	Cysteine desulfurase.	EC:2.8.1.7	-	0.0	73%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Thiamine metabolism	00730		0.0	73%

Source	GOs	Term	Type	Identity
Blast2go	cysteine metabolic process	GO:0006534	Biological Process	89%
Blast2go	iron-sulfur cluster assembly	GO:0016226	Biological Process	89%
Blast2go	pyridoxal phosphate binding	GO:0030170	Molecular Function	89%
Blast2go	cysteine desulfurase activity	GO:0031071	Molecular Function	89%
Blast2go	transaminase activity	GO:0008483	Molecular Function	89%
Blast2go	zinc ion binding	GO:0008270	Molecular Function	89%
Blast2go	mitochondrion	GO:0005739	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Blast2go	Aminotransferase, class V/Cysteine desulfurase	IPR000192	-	89%
Blast2go	Cysteine desulfurase	IPR010240	-	89%
Blast2go	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	IPR015421	-	89%
Blast2go	Pyridoxal phosphate-dependent transferase, major region, subdomain 2	IPR015422	-	89%
Blast2go	Pyridoxal phosphate-dependent transferase, major domain	IPR015424	-	89%
Blast2go	Cysteine desulfurase, NifS	IPR016454	-	89%
Blast2go	Aminotransferase class-V pyridoxal-phosphate binding site	IPR020578	-	89%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AA74

Fln msg:

Fln protein: MASKLLSVASRRAARTLTSGSPAARSLVNGHTAASFSTAAARAEEETVEETVEGITMKGVKIAGRPLYLDMQATSPVDPRVLDTMLPYYMYQYGNPHSRTHLFGWESDRAVEKAREQVAALIGASPKEIIFTSGATESNNISVKGVMHFFKDKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVKKDGLIDIDELRAAIRPDTGLVSVMTVHNEIGVIQPIAEIGEICRQNKIFLHTDAAQAAGKIPVNVDEMKVDLMSLSGHKIYGPKGIGALYMRRRPRVRVEPQMNGGGQERGIRSGTVATPLAVGMGAACELAMKEMAYDQSHVSALQERLLSGITAQLDGVEINGSAERRYAGNLNLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTPQEIDRTIKLTVDQVQKLREMSPLWEMVKEGIDIKNIQWSQH*

Protein Length: 467

Fln nts: CGTCGTGACCTTGGCTGTCATTGATGGAACCTAGTTTATAGTGGACAACGGACTGATAAACTTAATAAAGAAACCTCACAAATACATTTTGTTGCAGCACTGTTCAAACCCAACTCTAATCTTCGAATCCCGACACTTCATTTTTTCAGACTCCACTTCGGCAGCTCGGCA_-_ATGGCGTCGAAGCTCCTATCCGTGGCGTCAAGAAGGGCCGCCAGAACTCTCACCAGTGGCAGCCCGGCCGCCAGATCTCTTGTCAATGGCCACACGGCTGCCTCTTTCTCAACGGCGGCAGCGAGAGCAGAAGAAGAAACGGTGGAGGAAACTGTGGAAGGAATCACAATGAAGGGCGTCAAGATAGCAGGCCGGCCCCTGTATCTCGACATGCAGGCAACATCCCCAGTCGATCCTAGGGTTCTGGACACCATGCTGCCGTATTACATGTACCAATACGGAAACCCTCATTCTCGAACCCATCTCTTCGGCTGGGAATCCGATAGAGCCGTGGAAAAGGCCAGAGAGCAGGTGGCCGCCCTTATCGGCGCGAGCCCTAAAGAAATAATCTTTACCTCGGGTGCCACTGAATCGAACAACATATCCGTCAAGGGTGTGATGCATTTCTTCAAAGACAAGAAAAGACACGTCATCACTACGCAGACGGAGCACAAGTGCGTCCTGGACTCATGCCGACACTTGCAGCAGGAGGGTTTCGATGTCACCTACTTACCCGTGAAAAAAGACGGCCTCATAGACATCGACGAGCTGAGGGCCGCCATCAGGCCCGACACAGGTTTGGTCTCTGTCATGACTGTTCATAACGAAATTGGTGTGATTCAGCCCATTGCAGAAATAGGTGAAATATGTAGGCAAAACAAAATTTTCCTTCACACTGATGCGGCTCAGGCCGCTGGTAAAATTCCTGTCAATGTCGATGAGATGAAAGTGGATTTGATGTCATTGAGTGGGCACAAGATTTATGGGCCTAAGGGAATTGGGGCTCTGTATATGCGCCGCAGGCCCAGGGTTCGAGTCGAGCCGCAAATGAACGGCGGAGGGCAGGAGAGGGGGATCAGAAGTGGCACAGTAGCGACGCCGTTGGCTGTCGGCATGGGCGCTGCCTGTGAGTTGGCGATGAAAGAGATGGCTTATGACCAGAGTCATGTATCGGCGCTGCAGGAGCGATTGTTGAGTGGGATTACGGCGCAGCTTGATGGTGTTGAGATCAATGGGAGCGCGGAGCGTCGTTATGCGGGGAATCTCAATCTGTCTTTTGCTTATGTAGAGGGGGAGAGCCTTCTCATGGGTCTCAAGGAGGTTGCTGTCTCGAGCGGCAGCGCGTGCACGAGTGCCAGTCTTGAGCCCTCTTATGTTTTGAGGGCTTTGGGGGTTGAAGAAGACATGGCTCATACTTCTATTAGATTTGGGATTGGAAGGTTCACCACGCCACAAGAGATCGATAGAACCATTAAGCTTACTGTTGATCAGGTTCAGAAGCTGAGAGAGATGAGTCCGTTGTGGGAAATGGTGAAAGAAGGAATTGATATAAAAAACATCCAATGGTCGCAGCATTAG___AATGACTTTGTATTCACATGATCAAAAGGGCATATTTGCAACCATTGATTAATTCTCCATTATAAATGCTCTCCAGTTTGTAATATTCAAAACTCGACATATCGGTGGGTCACCTTTAACGTCCTTACAGATGCCAAGTTGTACACTCATCAAGCAAGTTTGATCTAGACAAGTATAGCCCCGCTGAGTTTAATGTATTTGTATAATCTTTGGTTCATATGTAAAATATGTTGTTTAATAGCGTGTCGGATTTTCAAGTCCAACTAAGCACTGTTTGGTATTGAGAAACTATAGAAATTATAGATCT

Fln Alignment:

sp_v1.1_unigene15677___MASKLLSVASRRAARTLTSGSPAARSLVNGHXXXXXXXXXXXXXXXXXXXXXEGITMKGVKIAGRPLYLDMQATSPVDPRVLDTMLPYYMYQYGNPHSRTHLFGWESDRAVEKAREQVAALIGASPKEIIFTSGATESNNISVKGVMHFFKDKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVKKDGLIDIDELRAAIRPDTGLVSVMTVHNEIGVIQPIAEIGEICRQNKIFLHTDAAQAAGKIPVNVDEMKVDLMSLSGHKIYGPKGIGALYMRRRPRVRVEPQMNGGGQERGIRSGTVATPLAVGMGAACELAMKEMAYDQSHVSALQERLLSGITAQLDGVEINGSAERRYAGNLNLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTPQEIDRTIKLTVDQVQKLREMSPLWEMVKEGIDIKNIQWSQH
D5AA74_______________MASKLLSMASRRAARTL----------VNGRLAASFSTAAARAVEETVEETVEGITMKGVKIAGRPLYLDMQATSPVDPRVLDAMMPFYMYQYGNPHSRTHLFGWESDTAVEKAREQVASLIGASPKEIIFTSGATESNNISIKGVMHFFKDKKRHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKKDGLIDIDELKAAIRPDTGLVSVMTVHNEIGVIQPIEEIGEICRQNKIFLHTDAAQAAGKIPVNVDEMKVDLMSLSGHKIYGPKGIGALYMRRRPRVRVEPQMNGGGQERGIRSGTVPTPLAVGMGAACELAMKEMAYDQRHVSALQERLLSGIKGQLDGVEINGSAERRYAGNLNLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTPQEIDRTIKLTVDQVQKLREMSPLWEMVKEGIDIKNIQWSQH

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
585	80	20			0.995493
586		20	80		0.995493
587		20	80		0.995493
588	20	80			0.995493
611	75			25	0.996473
618	25			75	0.996473
619			25	75	0.996473
621	75	25			0.996473
625	33			66	0.997452
627	33	66			0.997452
1035			85	14	0.999981
1040	14		85		0.999981
1049		85		14	0.999981
1130		7	14	78	0.999974
1154		11		88	0.923564
1166	88	11			0.923564
1184		87		12	0.992554
1185	87		12		0.992554
1190	12	12		75	0.975346
1193		12	87		0.992554
1196	12			87	0.992554
1204		12	87		0.992554
1217		85		14	0.993533
1229	85		14		0.993533
1268		82	17		1.0
1279		83		16	1.0
1295		11		88	0.999952
1307		86	13		0.999973
1313	85		14		0.999981
1343	85			14	0.999981
1352		85	14		0.999981
1364	29		70		1.0
1379		11		88	0.999952
1382	88		11		0.999952
1406			86	13	0.999973
1455			83	16	1.0
1457	16		83		1.0
1478		12		87	0.999964
1487			13	86	0.999973
1569			15	84	0.999986
1570	15	84			0.999986
1571			15	84	0.999986
1574			15	84	0.999986
1575	15			84	0.999986
1577	15			84	0.999986
1582	15			84	0.999986
1583	15	76		7	0.999983
1585		84	15		0.999986
1587		15	84		0.999986
1588	15	84			0.999986
1591	15	84			0.999986
1854	33			66	0.997452
1855		33	33	33	0.997753
1856	33	33	33		0.997753
1857	33	33	33		0.997753
1858	33		33		0.997753

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain