SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15587

Length: 2033 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene15587
CGTCGTGACCTTGGCTGTCACTCAATCTTCTTTCGGCTATTGATTGCCCTCCTCCCCGCTCCTCCACTTTGCCCACTTGAAAAAACGTTAAAGGGATTTGTTGGTCCAGCTACCCTCTTTGTATATATCTCGTTTAGCAGGCAGAGTGACCAGAGCATTACAAATCTTCGTCTTCAAGATTAGATTTTTCTCCGAACAGTTTGTTCGGCTCCCGAGTTCTTTGCTCTGTGTTTTAGTGACATTCTTCCTACCCAACCCAAACAAATATGCCGGGGCACTGTAGCCGGGTGGACTACTACTACTCAAATCCATCCTCTTCCTGGATTGAAGAAACCCACAAACGCATATTTGAAAAATCGATGGTTGCAGACAAAGACCTAGAGTGGAATCCTTTTCCATTGGAGGATGATTTCTTTGCCATCAGAAAGAAATGGCTCAGCGATTTCAATTCGAATGTGAAGAAGACCATAGAGAAGGAGCCCTTCACCTTTGCCTGCACAGATCGTATAGCAATGGAGATGGAGACAACTCCTTGTCACAAAACCATTCGCAGGTTTCGACAGACCACAGGAGGACGCGGGGATTCATTCGCTGTTCAAAGAATAGTAGAGAAAGCTCAGTTCCGCAGGGATGAATTCGTGAAGGACCTGGCTGATCGAATGACAGTGATGGAGATGAGTGTGGAAAAGCTCAAGGATTTGAAGGCCAAGAAGGAAACTAAACCTGAAAAAGCAAAGCCACTGCCATGTGCATGTACTGTGAAGAAGTCCTTAAAGCAAGAATGGAAGGGGGATAATGGTGTGAAGGGCAAGATTTCATACAAATGGGAAGAGTCTAAGAATAAAGTCACCGATCCCATCACAATAACAATTCCTGGAGCGCGGCAGGGGGAGTTCAAAACCATTCGAGTTGAAAAATATACTGTTATCAATGATACCAAGTCTTCAGATGCTAAGATCCGAGTCATTCCACTTGAAGAAGGCGGTGGGAGCAGCAGTAGCAGCAGCAGCCACTCAGATGATGATGAGGCGTGTAAGAAAATCCAGGGTGTGTTGGCCAAAAGGAGAGCTGCAGCAACTGGTCAACTCAGTTGTAAGGGGAAGCAGAAGGAGTTGTCTCCCCAGGATGCTGCTGTGATCATTCAGACCTGCTTTCGGGAATATCTGGTTCGTCGTTCCCAAAACCTTCGCAGCCTACGAGACCTTGCTATAGCAAAGGCCAAGCTGAAGGAGCTCAGATCTCTATTCACAAACTATGCCTATCGTCGGCATCTGTGCAGGGATGCAGAGGAGAAACAGAGATTTTCCGAGAAAACTATTGTCCTCTTACTTACAGTCGATTCCATTGAGGGTTCAAACCATTTGGTCAGGGAAGCAAGGAGGTCAATGATTCAAGAGTTGGAATCAATGCTGGATGCCGTGGATCCTCAGTCAGCCAACAAGGGTGCTGTTGGTTCCAGTAGAAGAAAATTTGATTTGCCTGAAGCTGGACCCCCTCAGAAGGAAATGGACGCGGAAGTTGCACAGGTTGTCACTGTGGTGGAAGAGGAGAATCTAGGAAGTCTGTTTTCATAATGTTACCTTGTGAAGGAACTGTGAGCCGAATTTCTACACAGAAAAATTCAATACCGGAATGAATGTTGGTCCAGTGTTATGATTTTCAATTCAGTTAGCTCAGTACATTTCCCAAGGTGATCTGGCAGACACATGGAAACTGAATTTCCAGTAGGGATTTTCTATCCAATTTTCACAATGTCTGTTTCCAGTACTAATCTGTTTTTCCATTAAATTCCAGTATGAATCCTTTCTTTAACTTTGGAAACTGAATTTCCAGCAGGGATTTTCTATCCAATTCTCACAATGTCTGTTTACAATACTAGTGTGTCTTTCCAATACTAAATTCCAGTATGAATCCTTCTTTAACTTTAGGGGGTACTGTCTTGTANTTAATACTTTAATGCAATTTAAGTTTGCACTAATAATTCTCTTTTCAATGATGTGGGACTGGGTGGGCTTTTGGCNCAATGAATTTCC

Ace file of the UniGene sp_v1.1_unigene15587

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	IQ calmodulin-binding motif family protein	-	-	5.0e-39	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	84%
Blast2go	iq calmodulin-binding motif family protein	-	-	0.0	76%

Source	GOs	Term	Type	Identity
Blast2go	protein folding	GO:0006457	Biological Process	76%
Blast2go	cellular response to heat	GO:0034605	Biological Process	76%
Blast2go	cellular response to unfolded protein	GO:0034620	Biological Process	76%
Blast2go	cellular response to cold	GO:0070417	Biological Process	76%
Blast2go	calmodulin binding	GO:0005516	Molecular Function	76%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	76%

Source	InterPros	Term	Type	e value	Identity
Blast2go	IQ motif, EF-hand binding site	IPR000048	-	0.0	76%
Blast2go	BAG domain	IPR003103	-	0.0	76%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABG1

Fln msg:

Fln protein: MPGHCSRVDYYYSNPSSSWIEETHKRIFEKSMVADKDLEWNPFPLEDDFFAIRKKWLSDFNSNVKKTIEKEPFTFACTDRIAMEMETTPCHKTIRRFRQTTGGRGDSFAVQRIVEKAQFRRDEFVKDLADRMTVMEMSVEKLKDLKAKKETKPEKAKPLPCACTVKKSLKQEWKGDNGVKGKISYKWEESKNKVTDPITITIPGARQGEFKTIRVEKYTVINDTKSSDAKIRVIPLEEGGGSSSSSSSHSDDDEACKKIQGVLAKRRAAATGQLSCKGKQKELSPQDAAVIIQTCFREYLVRRSQNLRSLRDLAIAKAKLKELRSLFTNYAYRRHLCRDAEEKQRFSEKTIVLLLTVDSIEGSNHLVREARRSMIQELESMLDAVDPQSANKGAVGSSRRKFDLPEAGPPQKEMDAEVAQVVTVVEEENLGSLFS*

Protein Length: 437

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATCTTCTTTCGGCTATTGATTGCCCTCCTCCCCGCTCCTCCACTTTGCCCACTTGAAAAAACGTTAAAGGGATTTGTTGGTCCAGCTACCCTCTTTGTATATATCTCGTTTAGCAGGCAGAGTGACCAGAGCATTACAAATCTTCGTCTTCAAGATTAGATTTTTCTCCGAACAGTTTGTTCGGCTCCCGAGTTCTTTGCTCTGTGTTTTAGTGACATTCTTCCTACCCAACCCAAACAAAT_-_ATGCCGGGGCACTGTAGCCGGGTGGACTACTACTACTCAAATCCATCCTCTTCCTGGATTGAAGAAACCCACAAACGCATATTTGAAAAATCGATGGTTGCAGACAAAGACCTAGAGTGGAATCCTTTTCCATTGGAGGATGATTTCTTTGCCATCAGAAAGAAATGGCTCAGCGATTTCAATTCGAATGTGAAGAAGACCATAGAGAAGGAGCCCTTCACCTTTGCCTGCACAGATCGTATAGCAATGGAGATGGAGACAACTCCTTGTCACAAAACCATTCGCAGGTTTCGACAGACCACAGGAGGACGCGGGGATTCATTCGCTGTTCAAAGAATAGTAGAGAAAGCTCAGTTCCGCAGGGATGAATTCGTGAAGGACCTGGCTGATCGAATGACAGTGATGGAGATGAGTGTGGAAAAGCTCAAGGATTTGAAGGCCAAGAAGGAAACTAAACCTGAAAAAGCAAAGCCACTGCCATGTGCATGTACTGTGAAGAAGTCCTTAAAGCAAGAATGGAAGGGGGATAATGGTGTGAAGGGCAAGATTTCATACAAATGGGAAGAGTCTAAGAATAAAGTCACCGATCCCATCACAATAACAATTCCTGGAGCGCGGCAGGGGGAGTTCAAAACCATTCGAGTTGAAAAATATACTGTTATCAATGATACCAAGTCTTCAGATGCTAAGATCCGAGTCATTCCACTTGAAGAAGGCGGTGGGAGCAGCAGTAGCAGCAGCAGCCACTCAGATGATGATGAGGCGTGTAAGAAAATCCAGGGTGTGTTGGCCAAAAGGAGAGCTGCAGCAACTGGTCAACTCAGTTGTAAGGGGAAGCAGAAGGAGTTGTCTCCCCAGGATGCTGCTGTGATCATTCAGACCTGCTTTCGGGAATATCTGGTTCGTCGTTCCCAAAACCTTCGCAGCCTACGAGACCTTGCTATAGCAAAGGCCAAGCTGAAGGAGCTCAGATCTCTATTCACAAACTATGCCTATCGTCGGCATCTGTGCAGGGATGCAGAGGAGAAACAGAGATTTTCCGAGAAAACTATTGTCCTCTTACTTACAGTCGATTCCATTGAGGGTTCAAACCATTTGGTCAGGGAAGCAAGGAGGTCAATGATTCAAGAGTTGGAATCAATGCTGGATGCCGTGGATCCTCAGTCAGCCAACAAGGGTGCTGTTGGTTCCAGTAGAAGAAAATTTGATTTGCCTGAAGCTGGACCCCCTCAGAAGGAAATGGACGCGGAAGTTGCACAGGTTGTCACTGTGGTGGAAGAGGAGAATCTAGGAAGTCTGTTTTCATAA___TGTTACCTTGTGAAGGAACTGTGAGCCGAATTTCTACACAGAAAAATTCAATACCGGAATGAATGTTGGTCCAGTGTTATGATTTTCAATTCAGTTAGCTCAGTACATTTCCCAAGGTGATCTGGCAGACACATGGAAACTGAATTTCCAGTAGGGATTTTCTATCCAATTTTCACAATGTCTGTTTCCAGTACTAATCTGTTTTTCCATTAAATTCCAGTATGAATCCTTTCTTTAACTTTGGAAACTGAATTTCCAGCAGGGATTTTCTATCCAATTCTCACAATGTCTGTTTACAATACTAGTGTGTCTTTCCAATACTAAATTCCAGTATGAATCCTTCTTTAACTTTAGGGGGTACTGTCTTGTAaTTAATACTTTAATGCAATTTAAGTTTGCACTAATAATTCTCTTTTCAATGATGTGGGACTGGGTGGGCTTTTGGCcCAATGAATTTCC

Fln Alignment:

sp_v1.1_unigene15587___MVADKDLEWNPFPLEDDFFAIRKKWL---------SDFNSNVKKTIEKEPFTFACTDRIAMEMETTPCHKTIRRFRQTTGGRGDSFAVQRIVEKAQFRRDEFVKDLADRMTVMEMSVXXXXXXXXXXXXXXXXXXXXXCACTVKKSLKQEWKGDNGVKGKISYKWEESKNKVTDPITITIPGARQGEFKTIRVEKYTVINDTKSSDAKIRVIPLXXXXXXXXXXXXXXXXXXACKKIQGVLAKRRAAATGQLSCKGKQKELSPQDAAVIIQTCFREYLVRRSQNLRSLRDLAIAKAKLKELRSLFTNYAYRRHLCRDAEEKQRFSEKTIVLLLTVDSIEGSNHLVREARRSMIQELESMLDAVDPQSANKGAVGSSRRKFDLPEAGPPQKEMDXXXXXXXXXXXXXNLGSLFS
D5ABG1_______________MVSDIDLEWNPFPLEDDFFASRKKWLCERELSRWISDFNLNVKKAIEKEPFTFTFTDRIATEMEISPFHKTIRRLRSTTGRRGDSFAVQRIVEKAQFHRDEFVHDLTERVAAMEMSVEKLMDLKAKKDTKFEKAKPLPCACNVKKSFKKEWKGENGVKGKFSYKYEESKKKITDPITIIIPGAREGESKTIRVEKYTVINDTKSSDAKIRVIPLEECDGSSSSSSSHTDDDEASKKIQGVLARRRAAANAQLSWKGKKKELSPHDAAAIIQTCFREYLARRSQNLRSLRDLAVAKAKLKELRSLFTNYAYRRHLCRDAEEKQRFSEKTIVLLLTVDSIEGSNHLVREARRSMIQELESMLDAVDPQSPNKGAAGSNRRKFDLPESAPPQKEMETEVAQVVTVVDEENVGSLFS

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
1445	10			90	0.90298
1446			90	10	0.90298
1447		90		10	0.90298
1554		47	52		1.0
1563		47		52	1.0
1581		87	12		1.0
1583		12		87	1.0
1588	87	12			1.0
1589			87	12	1.0
1590			87	12	1.0
1591	87		12		1.0
1592	87		12		1.0
1597		12	87		1.0
1599		8	87		0.999414
1600		91		8	0.999766
1602	8	4	87		0.999414
1748		88		11	0.999937
1762		90		10	0.999898
1773		90	10		0.999898
1776		5		77	1.0
1885		84		15	0.999986
1891	72	27			1.0
1945			40	60	1.0

SSRs (tot: 2)

Start position	End position	Sequence	Length
292	303	ACT ACT ACT ACT	12
999	1010	AGC AGC AGC AGC	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain