SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15547

Length: 1678 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene15547 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	dead box ATP-dependent RNA helicase, putative; K03257 translation initiation factor eIF-4A	-	-	86%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	protein	-	-	93%

Source	GOs	Term	Type	Identity
Blast2go	auxin biosynthetic process	GO:0009851	Biological Process	93%
Blast2go	ATP-dependent helicase activity	GO:0008026	Molecular Function	93%
Blast2go	ATP binding	GO:0005524	Molecular Function	93%
Blast2go	translation initiation factor activity	GO:0003743	Molecular Function	93%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PRI2

Fln msg: Overlapping hits, possible frame ERROR between 1407 and 1277,

Fln protein: MAAADQAGNNFDAKSYDKNMQSVLGGEELYSTWEEVHETFDSMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCQGLDVIQQAQSGTGKTATFCSGILQQLDYSMPECQALVLAPTRELAQQIEKVMRALGDYLQVRVHACVGGTSIREDLRILQAGVHVVVGTPGRVYDMLRRRALRPDSIKMFVLDEADEMLSRGFKDQIYDIFQLLPANLQVGVFSATMPPEALEITRKFMTNPVRILVKRDELTLEGIKQFYVNVEREDWKLDTLCDLYETLAITQSVIFINTRRKVDWLTDQMRARDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxICTMPCAVS*

Protein Length: 431

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAGTCCCGGATAGATAGATTTCCTCGCTTCCTTTAAGGACCTCTGTATTGTTTTTTGATCTTTGAGGGGGTTTATTACAATACTACTGCAGATCAAGGGTTTCTCAGCGAAAAGGTAAAACTAGGAA_-_ATGGCAGCTGCGGATCAGGCCGGAAACAACTTCGACGCCAAGAGCTATGACAAAAACATGCAGAGCGTCCTTGGAGGAGAAGAACTTTACTCTACCTGGGAGGAGGTTCATGAAACTTTTGACTCCATGGGGCTTCAGGAAAACCTTTTAAGAGGAATTTATGCATATGGTTTTGAGAAGCCATCTGCCATCCAGCAGAGAGGGATTGTTCCTTTCTGCCAAGGTCTTGATGTTATCCAGCAAGCACAATCAGGAACAGGAAAGACTGCAACTTTCTGTTCAGGAATTCTACAACAGTTGGACTACAGTATGCCTGAGTGTCAAGCTCTAGTTTTGGCCCCGACACGAGAACTAGCTCAGCAGATTGAGAAAGTTATGAGGGCACTTGGTGATTACCTTCAAGTAAGAGTACATGCATGTGTTGGTGGGACAAGCATCCGGGAGGACCTCCGTATTCTCCAAGCTGGTGTTCATGTTGTTGTGGGGACACCAGGTAGGGTCTATGATATGCTGCGGCGGCGTGCCTTGCGTCCAGACTCAATCAAAATGTTTGTGCTTGATGAAGCAGATGAAATGCTATCACGTGGATTCAAGGATCAGATATATGATATCTTTCAATTGCTACCTGCAAATCTCCAAGTTGGTGTTTTCTCTGCCACCATGCCTCCTGAGGCCTTGGAGATTACGAGGAAGTTCATGACTAATCCGGTCAGGATTCTTGTGAAAAGAGACGAGCTTACGCTGGAGGGTATCAAACAATTCTATGTTAATGTAGAGCGAGAGGATTGGAAGCTTGATACACTATGTGATCTGTATGAAACTTTGGCAATCACACAGAGTGTGATTTTCATCAATACCAGGAGGAAGGTAGATTGGCTGACAGATCAAATGCGAGCTCGGGATCACACAGTTTCTGCAACCCATGGTGACATGGATCAGAACACAAGAGACATCATTATGAGGGAATTCAGGTCTGGATCTTCTCGAGTCCTGATCACCACTGATCTATTGGCCCGAGGTATTGATGTACAACAAGTGTCTCTTGTTATCAACTATGACTTACCAACACAGCCTGAAAACTACCTTCACCGTATTGGTCGGAGTGGACGATTTGGCAGGAAGTGTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxATCTGTACTATGCCGTGTGCAGTGAGTTGA___GTACGTTATCTTTTGGGTTGAATACTCTTCCCCTCTTGATTCTGTAAATTGTCAACAACTATTTTGTTCGGTGAAACATTCTGTATTTCTTGGACCTTGCTTAACGGTATTGCTTTGTTGTAATCTTATCTGAGAGGGAGCCATTTTAAGCTCTTTGCTCGCCCTCTCAGGGTTTTATAAGTTTATTCATAATACAAGGTTGAGAGTGTAGTAACTGTTTTCTGCACTTGGATGTTATGC

Fln Alignment:

sp_v1.1_unigene15547___MAAADQAGNNFDAKSYDKNMQSVLGGEELYSTWEEVHETFDSMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCQGLDVIQQAQSGTGKTATFCSGILQQLDYSMPECQALVLAPTRELAQQIEKVMRALGDYLQVRVHACVGGTSIREDLRILQAGVHVVVGTPGRVYDMLRRRALRPDSIKMFVLDEADEMLSRGFKDQIYDIFQLLPANLQVGVFSATMPPEALEITRKFMTNPVRILVKRDELTLEGIKQFYVNVEREDWKLDTLCDLYETLAITQSVIFINTRRKVDWLTDQMRARDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKCGGHQLxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxICTMPCAVS
C0PRI2_______________MAGVDQAGNNFDAKSYDKNMQSVLGGEELYSTWEEVHETFDSMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCQGLDVIQQAQSGTGKTATFCSGILQQLNYNVPECQALVLAPTRELAQQIEKVMRALGDYLQVRVHACVGGTSIREDLRILQAGVHVVVGTPGRVYDMLRRRALRPDSIKMFVLDEADEMLSRGFKDQIYDIFQLLPTNLQVGVFSATMPPEALEITRKFMTNPVRILVKRDELTLEGIKQFYVNVEREDWKLDTLCDLYETLAITQSVIFINTRRKVDWLTDQMRARDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRK--GVAIxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxICTMPCAVN

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
1064		88		11	0.923564
1065		90	10		0.90298
1087	9	72			0.999994
1088		18		81	0.999994
1093		10	90		0.90298
1303	71		28		1.0
1619		20		80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain