SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15441

Length: 2411 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene15441
CGTCGTGACCTTGGCTGTCACTCAATTTGGCGATCCCCTATAGAATATCGTCGTGACCTTGGCTGTCACTCAGATTTGCCCTTGCAGTGTCGTGTGAAACTTACAAAGATTGTGCCTCTATAAATACAAGGACCTGGGTTTTAATTTCTTCGATTGCTTCGTAGCGTGAGATTCAGGGTTTGTAAGCTTAGGGCTTCTTTTCCGAAGGGTTGGATGCGCTGATCAATCGCTGCTGTGCGGGACTTCTTCCGAAGAAGATTTCAAAGGTGAGTCCTATTGGTTGTCAATCGCATTCAAAGTGGAGGGTGTACAGGTTGAATATTCTTTTATGTCTGAAGCCGTTTGTTCATATTTGGGGCTGATTGGGCTGCTTGGCGAATACTGTCCTACATCAAAGAAAAAGAATAAACAGCTTCGTCTTTGTAGCAAAAAATTTTGGCTGTATGCAGCCAAAGCCAAGTCAGAAGTCGAGGTTGATTTGGGTTTGCAGTTGGATGCACCCATTTTTCCACGTGCAAGGTGGAGTGTTAAAACATCATAGAGAAAGTCCCTTTTCCCCGCAGGCATTTGGTGGCCTTTTACATACTCTAGTATCTAACTCTTGATAGAAACGGTTCTTCAAATTCCAAATTCACTCTTGCATTCCACTTAATCACTAATTAGAAAATATGGCTCTTCTAAATATTGGCGAGGGCAACAAAGATGATGCCTTTTACAGGTATAAGATGCCTAAGCTGATTACAAAAATTGAGGGACGAGGTAATGGCATAAAGACAAATCTTGTCAATATGGTAGATGTGGCTAAAGCTCTTGCCAGACCCCCTTCATATACAACAAAGTATTTCGGCTGTGAACTGGGTGCGCAATCAAAGTTTGATGAAAAATCTGGGACTGCTCTTGTCAATGGTGCTCATGATAGTTCTAAACTTGTAGCTTTGTTGGAGATTTTTATCAAGAAGTATGTGCAGTGCTATGGATGTGGCAATCCTGAGACAGAGATCCTTATTTCAAAAACACAAATGATTTCACTGAAGTGTGCTGCATGTGGGTATGTTTCAGATGTTGATATGAGGGACAAGCTTACTACGTTTATTTTGAAGAATCCGCCAGAGCAGAAGAAGGGAGGCAAAGATAAGAAGGCCATGAGAAGAGCAGAAAAGGAACGTTTGAAAGCAGGAGAGGCTATTGATGAAGAAGCTAAAAAATCCAAGAAAGAATCCAAGAAGAAGGGCCACGAGGTTGCATCCAAGGTTGCTAAGAAGAAAACTAGTTCTGACGAAGACCGTTCTCCTCCTGAAAGCCATGTTGATGAAAACGAGGAGCTGGATGAGAATGATGATGTGCAATGGGCTACTGATACTTCTCTTGAAGCCGCACAGCAGCGTATCAGAGAGCAGTTGACAGCTGCTACAAGTGAAATGGTCATGTTGGACAGCCAGGAAGAACAAAAGTCGAAGTCACCAAAGAAGCCTTCAGGCAAGAATGAAAAGGTTGAGCTTGATAAGAAATCTCAAGCTGAAAACGGTGATGGGGTGTCTGAGAAGCCCGTCAAGGATGAAAAGGTTGAGCTTGATAACAATACTCAAGCTGAAAACGGTGATGAGGTATTTGAGAAGCCCATTCAACAGGATATTGAGGAGGAGACTGTTCTGACCAAATTGAACACATTGAGCATTCATGATGAGCTTGTCCTTGCTTTCAAGGAGCGCTTGAAGATTGGAGATTCTCCGAAAAAATTACAATCCTACGTACAATCTTCAAATGGTTCTCCACAGGAAATCATGAATGCCTATTTTGAAGCTCTTTTTGAGGGCGTCGGAAGAGGATTTTCAAAAGAGGCAGTTATGAAGAAAGGCTATCTATCCAGAGTTGTGCAAGATGAAAATTCTCAGTCGATGCTGCTTGGTGCTATTGAGGCATTTTGTAACCATGCACGAGCTGAAGCGGTCAAAGAGGTTGCTTTAGTTCTTAAATTTTTATATGATGAGGACATCTTGGAGGAAGACATCATTTTCCAATGGTATGATAAAAGTTCAGCTGGAAACACCTCACAAATATGGAAAAGTGTCAAACCATTTGTTGAGTGGCTGAAGAGTGCCGAGGCTGAATCAGATGAAGAATAAGTCTTTATCCTCTTTGGAAAGTTACCTGCATTCTAGGAATCTTACAGTACTGCTAGGAACTTATGATTCTCCAATATAAGGCTGAGGACTGAAGCTACGAGTGATATATGCCGTTTATATATTTGAGTATTTGCTGTGCTATCACTAGTGATATAACGTTCATATATTTTCAGTATTTGGGGTGCTGTCATGAGTGATATTTTATTTTACAGTTTTGTGTGTTTGGTTTATGGAGTTGAATAGATAGGAACCTACATTCGATATTATTTTTAGTTTTTGGTGTTGAAT

Ace file of the UniGene sp_v1.1_unigene15441 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	EIF5, eIF-5; Eukaryotic initiation factor-5; K03262 translation initiation factor eIF-5	-	-	56%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	82%
Blast2go	eukaryotic translation initiation factor 5	-	-	69%

Source	GOs	Term	Type	e value	Identity
Blast2go	binding	GO:0005488	Molecular Function	0.0	69%

Source	InterPros	Term	Type	Identity
Blast2go	Translation initiation factor IF2/IF5	IPR002735	-	69%
Blast2go	eIF4-gamma/eIF5/eIF2-epsilon	IPR003307	-	69%
Blast2go	MIF4-like, type 1/2/3	IPR016021	-	69%
Blast2go	Armadillo-type fold	IPR016024	-	69%
Blast2go	Translation initiation factor IF2/IF5, N-terminal	IPR016189	-	69%
Blast2go	Translation initiation factor IF2/IF5, zinc-binding	IPR016190	-	69%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A851

Fln msg: your sequence is longer than subject: 485 - 454

Fln protein: MALLNIGEGNKDDAFYRYKMPKLITKIEGRGNGIKTNLVNMVDVAKALARPPSYTTKYFGCELGAQSKFDEKSGTALVNGAHDSSKLVALLEIFIKKYVQCYGCGNPETEILISKTQMISLKCAACGYVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKAGEAIDEEAKKSKKESKKKGHEVASKVAKKKTSSDEDRSPPESHVDENEELDENDDVQWATDTSLEAAQQRIREQLTAATSEMVMLDSQEEQKSKSPKKPSGKNEKVELDKKSQAENGDGVSEKPVKDEKVELDNNTQAENGDEVFEKPIQQDIEEETVLTKLNTLSIHDELVLAFKERLKIGDSPKKLQSYVQSSNGSPQEIMNAYFEALFEGVGRGFSKEAVMKKGYLSRVVQDENSQSMLLGAIEAFCNHARAEAVKEVALVLKFLYDEDILEEDIIFQWYDKSSAGNTSQIWKSVKPFVEWLKSAEAESDEE*

Protein Length: 486

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTGGCGATCCCCTATAGAATATCGTCGTGACCTTGGCTGTCACTCAGATTTGCCCTTGCAGTGTCGTGTGAAACTTACAAAGATTGTGCCTCTATAAATACAAGGACCTGGGTTTTAATTTCTTCGATTGCTTCGTAGCGTGAGATTCAGGGTTTGTAAGCTTAGGGCTTCTTTTCCGAAGGGTTGGATGCGCTGATCAATCGCTGCTGTGCGGGACTTCTTCCGAAGAAGATTTCAAAGGTGAGTCCTATTGGTTGTCAATCGCATTCAAAGTGGAGGGTGTACAGGTTGAATATTCTTTTATGTCTGAAGCCGTTTGTTCATATTTGGGGCTGATTGGGCTGCTTGGCGAATACTGTCCTACATCAAAGAAAAAGAATAAACAGCTTCGTCTTTGTAGCAAAAAATTTTGGCTGTATGCAGCCAAAGCCAAGTCAGAAGTCGAGGTTGATTTGGGTTTGCAGTTGGATGCACCCATTTTTCCACGTGCAAGGTGGAGTGTTAAAACATCATAGAGAAAGTCCCTTTTCCCCGCAGGCATTTGGTGGCCTTTTACATACTCTAGTATCTAACTCTTGATAGAAACGGTTCTTCAAATTCCAAATTCACTCTTGCATTCCACTTAATCACTAATTAGAAAAT_-_ATGGCTCTTCTAAATATTGGCGAGGGCAACAAAGATGATGCCTTTTACAGGTATAAGATGCCTAAGCTGATTACAAAAATTGAGGGACGAGGTAATGGCATAAAGACAAATCTTGTCAATATGGTAGATGTGGCTAAAGCTCTTGCCAGACCCCCTTCATATACAACAAAGTATTTCGGCTGTGAACTGGGTGCGCAATCAAAGTTTGATGAAAAATCTGGGACTGCTCTTGTCAATGGTGCTCATGATAGTTCTAAACTTGTAGCTTTGTTGGAGATTTTTATCAAGAAGTATGTGCAGTGCTATGGATGTGGCAATCCTGAGACAGAGATCCTTATTTCAAAAACACAAATGATTTCACTGAAGTGTGCTGCATGTGGGTATGTTTCAGATGTTGATATGAGGGACAAGCTTACTACGTTTATTTTGAAGAATCCGCCAGAGCAGAAGAAGGGAGGCAAAGATAAGAAGGCCATGAGAAGAGCAGAAAAGGAACGTTTGAAAGCAGGAGAGGCTATTGATGAAGAAGCTAAAAAATCCAAGAAAGAATCCAAGAAGAAGGGCCACGAGGTTGCATCCAAGGTTGCTAAGAAGAAAACTAGTTCTGACGAAGACCGTTCTCCTCCTGAAAGCCATGTTGATGAAAACGAGGAGCTGGATGAGAATGATGATGTGCAATGGGCTACTGATACTTCTCTTGAAGCCGCACAGCAGCGTATCAGAGAGCAGTTGACAGCTGCTACAAGTGAAATGGTCATGTTGGACAGCCAGGAAGAACAAAAGTCGAAGTCACCAAAGAAGCCTTCAGGCAAGAATGAAAAGGTTGAGCTTGATAAGAAATCTCAAGCTGAAAACGGTGATGGGGTGTCTGAGAAGCCCGTCAAGGATGAAAAGGTTGAGCTTGATAACAATACTCAAGCTGAAAACGGTGATGAGGTATTTGAGAAGCCCATTCAACAGGATATTGAGGAGGAGACTGTTCTGACCAAATTGAACACATTGAGCATTCATGATGAGCTTGTCCTTGCTTTCAAGGAGCGCTTGAAGATTGGAGATTCTCCGAAAAAATTACAATCCTACGTACAATCTTCAAATGGTTCTCCACAGGAAATCATGAATGCCTATTTTGAAGCTCTTTTTGAGGGCGTCGGAAGAGGATTTTCAAAAGAGGCAGTTATGAAGAAAGGCTATCTATCCAGAGTTGTGCAAGATGAAAATTCTCAGTCGATGCTGCTTGGTGCTATTGAGGCATTTTGTAACCATGCACGAGCTGAAGCGGTCAAAGAGGTTGCTTTAGTTCTTAAATTTTTATATGATGAGGACATCTTGGAGGAAGACATCATTTTCCAATGGTATGATAAAAGTTCAGCTGGAAACACCTCACAAATATGGAAAAGTGTCAAACCATTTGTTGAGTGGCTGAAGAGTGCCGAGGCTGAATCAGATGAAGAATAA___GTCTTTATCCTCTTTGGAAAGTTACCTGCATTCTAGGAATCTTACAGTACTGCTAGGAACTTATGATTCTCCAATATAAGGCTGAGGACTGAAGCTACGAGTGATATATGCCGTTTATATATTTGAGTATTTGCTGTGCTATCACTAGTGATATAACGTTCATATATTTTCAGTATTTGGGGTGCTGTCATGAGTGATATTTTATTTTACAGTTTTGTGTGTTTGGTTTATGGAGTTGAATAGATAGGAACCTACATTCGATATTATTTTTAGTTTTTGGTGTTGAAT

Fln Alignment:

sp_v1.1_unigene15441___MALLNIGEGNKDDAFYRYKMPKLITKIEGRGNGIKTNLVNMVDVAKALARPPSYTTKYFGCELGAQSKFDEKSGTALVNGAHDSSKLVALLEIFIKKYVQCYGCGNPETEILISKTQMISLKCAACGYVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKAGEAIDXXXXXXXXXXXXKGHEXXXXXXXXXXXSDEDRSPPESHVXXXXXXXXXXDVQWATDTSLEAAQQRIREQLTAATSEMVMLDXXXXXXXXXXXXXSGKNEKVELDKKSQAENGDGVSEKPVKDEKVELDNNTQAENGDEVFEKPIQQDIEEETVLTKLNTLSIHDELVLAFKERLKIGDSPKKLQSYVQSSNGSPQEIMNAYFEALFEGVGRGFSKEAVMKKGYLSRVVQDENSQSMLLGAIEAFCNHARAEAVKEVALVLKFLYDEDILEEDIIFQWYDKSSAGNTSQIWKSVKPFVEWLKSAEAESDEE
D5A851_______________MALLNIGEGNRDDAFYRYKMPKLMTKIEGRGNGIKTNLVNMVDVAKALARPPSYTTKYFGCELGAQSKFDEKTGIALVNGAHDNSKLVALLEIFIKKYVQCYGCGNPETEILISKTQMISLKCAACGHISDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKAGEAIDEENKKVKKESKKKGHEVVSKVSKKKTGSDEDRSPPGSHV---DENEDDDEVQWETNTSLEAAQQRIREQLTSATSEMVMLD--TLEEPKPTKKSSGKNEKVEVDKKSKAENGD------------------------EVAEKPMEKDV-EETVLSKLNTISIHDELLVAFKERLKNGDTPKQLQNYMQSSDGSPQEIMNAYFEALFEGVGKGFSKEVSSKKGYLSGVVQDENSQSMLLGAIEAFCNHARAEAVKEVALVLKTLYDEDILEEDIIFQWYDKGSTGNASQIWKNVKPFVEWLKSAEAESDEE

SNPs (tot: 67)

Position	A %	C %	G %	T %	Probability
448		88	11		1.0
451	88	11			1.0
457		88	11		1.0
458		85	14		1.0
461			2	85	1.0
462	12			87	1.0
463		12		87	1.0
466	2	88			0.999999
487			5	94	0.998744
491	91	5		2	0.995208
498	5	2		91	0.99441
500	94		5		0.998578
503	91	2	5		0.995208
514			90	9	1.0
515		93	6		0.999153
517			6	90	0.997073
518		93		6	0.999153
522	6			90	0.996531
524	93	6			0.999153
525		6	93		0.999153
528			6	93	0.999153
530		6		93	0.999153
531	6			93	0.999153
533	93		6		0.999153
534	6			93	0.999153
536			6	93	0.999153
652			13	86	1.0
654		8	91		0.999807
1208	11			66	0.999999
1209	11			88	0.923564
1211		11		88	0.923564
1925		33	66		1.0
2266	2	4		90	0.960255
2268		25	47		1.0
2269	44	34			1.0
2270	55	10		31	1.0
2271		13	76	10	1.0
2272	75	3			1.0
2273	65	6	25	3	1.0
2274	58		12		1.0
2276			46	53	1.0
2278	63	33		2	1.0
2280	66		33		1.0
2283	33	61		5	1.0
2288		94	5		0.998578
2289	30	47	2		1.0
2290	11	38	5	44	1.0
2291	8	13	22	55	1.0
2292	22	2	5	11	1.0
2294		8	91		1.0
2295	11			76	1.0
2297		11	11	76	1.0
2298			20	80	1.0
2300	86	13			1.0
2301	13			86	1.0
2302	86	13			1.0
2305		13	86		1.0
2347	25			75	0.996473
2348	25	50		25	0.995533
2349	25			75	0.996473
2353			25	75	0.996473
2354	25		75		0.996473
2356		75		25	0.996473
2358			75	25	0.996473
2359		75	25		0.996473
2364		25	75		0.996473
2365			25	75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain