SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15376

Length: 1870 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene15376

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glutamine synthetase n=6 Tax=Spermatophyta RepID=GLNA1_ALNGL	-	-	84%
FL-Next	sp=Glutamine synthetase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	cytosolic glutamine synthetase	-	-	92%

Source	ECs	Term	Type	e value	Identity
Blast2go	Glutamate--ammonia ligase.	EC:6.3.1.2	-	0.0	92%

Source	KEGGs	Term	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250	92%
Blast2go	Arginine and proline metabolism	00330	92%
Blast2go	Nitrogen metabolism	00910	92%
Blast2go	Metabolic pathways	01100	92%

Source	GOs	Term	Type	Identity
Blast2go	nitrogen fixation	GO:0009399	Biological Process	92%
Blast2go	glutamine biosynthetic process	GO:0006542	Biological Process	92%
Blast2go	glutamate-ammonia ligase activity	GO:0004356	Molecular Function	92%
Blast2go	ATP binding	GO:0005524	Molecular Function	92%
Blast2go	cytoplasm	GO:0005737	Cellular Component	92%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Glutamine synthetase, catalytic domain	IPR008146	-	0.0	92%
Blast2go	Glutamine synthetase/guanido kinase, catalytic domain	IPR014746	-	0.0	92%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NSW2

Fln msg: Overlapping hits, possible frame ERROR between 524 and 488,

Fln protein: MSLLTDLINLDLSDVTEKIIAEYIWIGGSGMDIRSKARTLSHPVTDPKDLPKWNYDGSSTGQAPGKDSEVILxxxxxxxxxxxxxxNILVICDTYTPAGEPIPTNKRANAAKIFSHPDVVVEEPWYGIEQEYTLLQKDVNWPLGWPVGGYPGPQGPYYCGTGADKAYGRDIVDAHYKACLYAGINISGINGEVMPGQWEFQVGPTVGISSGDQVWAARYLLERITEVAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMREEGGIKVIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEREGKGYFEDRRPASNMDPYIVTSMIAETTILLK*

Protein Length: 357

Fln nts: TCCTGTCTTCACAAAGAGAGGACATTGGTTATGGAGAATTTATATTTATAGACCTGCCTTTTTGTCTTAAAAACAGATAGAGGTTTCTTAATTCCCCATCTTCTTTCATTGGTTTCTATAAATTCTTCCTCAGGTTGGGGTTGCCCTTTGCATCAATTGCTATAAATTCTTATTTCAGTGGCCTTTATTTCGAAATAGCAGATCAAAGGCCTTCACTGCTTGCAGAATTATACTTGTGCGGGAGTCTGTGATTTTGTTGTACATCCAAG_-_ATGTCTCTACTGACGGATTTGATCAACTTGGACCTCTCTGATGTCACTGAGAAGATCATCGCTGAGTACATATGGATCGGAGGCTCTGGCATGGATATCCGCAGCAAGGCCAGGACCTTATCTCACCCAGTTACGGACCCCAAAGATCTACCCAAGTGGAATTATGATGGATCCAGTACTGGACAGGCTCCTGGAAAGGATAGTGAAGTCATCCTTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAACATCTTGGTGATTTGTGATACATATACCCCAGCTGGAGAACCTATTCCTACTAACAAGAGAGCAAATGCTGCAAAAATATTTAGCCATCCCGATGTTGTTGTCGAGGAACCATGGTACGGGATTGAACAAGAATACACTCTTCTGCAAAAGGATGTGAATTGGCCTCTTGGATGGCCCGTAGGTGGTTACCCTGGTCCTCAGGGTCCTTATTATTGTGGAACTGGAGCAGACAAAGCCTACGGCCGTGATATCGTCGATGCCCACTATAAGGCTTGCCTGTATGCAGGAATCAACATTAGTGGCATCAATGGAGAAGTCATGCCCGGTCAATGGGAATTTCAAGTTGGCCCGACGGTTGGTATTTCATCTGGTGATCAAGTCTGGGCTGCACGTTACCTTCTTGAGAGAATCACAGAAGTGGCTGGTGTTGTCCTCTCATTTGACCCCAAACCCATTCAGGGTGATTGGAATGGTGCTGGTGCTCACACTAACTACAGTACGAAATCAATGAGAGAAGAAGGGGGAATTAAAGTGATCAAAAAGGCCATTGAAAAGTTAGGGTTGAGGCATAAGGAACACATTGCTGCCTATGGAGAGGGCAACGAGAGGCGTTTGACTGGCCGACATGAGACAGCAGACATAAACACATTTTCATGGGGAGTTGCAAATCGTGGAGCTTCTATTCGAGTTGGCCGTGACACGGAACGTGAAGGCAAAGGGTACTTCGAAGACCGCAGGCCAGCTTCCAACATGGACCCCTATATTGTAACATCTATGATTGCTGAGACAACCATCCTTTTGAAGTGA___GAGTAACATTGTTTACTGAATGAATAAAGATGCCGATACGATTGAAGTGTTCTTGATGCTAGTCAAATTGCGAAGGGATCCCCAATTGTTTGTGGGGGATATTCTCATTTGAATTTCTCTCTGTGCCTGAAGTATTTCCCCTATTTCTGTTAATAAGAACATTCTAGAAATAGGACTTGAGATTTAGGGTGCATTATATTCAGTGTCTAGTTTGTCATTCAGATTTGCATTATTCCATGACTCTGATATGATTGGTGTGCAGTTGAATTTAATGAATTCAGAAGTTCTTTTATTGCTTGCGAACGTTGGAGTTATTTCTTGCTGTCTTCTACTTTTTGGAAACATTGACAGAGCTTGGAAGGCTCTTTGTTTCTTATGGTTAAGTAATGTGACAAGAAATCCTTAAGCAGAACATCTGAGGGGGTTTTCCTGGGTTATAATTCCCAAAATATGATAAAAGAAATTAACTAAATCTCTTTCTACTTCTTGTTTGAGATGTAGTTCCAAATATAATGACAAAAACCAACAAACCAT

Fln Alignment:

sp_v1.1_unigene15376___MSLLTDLINLDLSDVTEKIIAEYIWIGGSGMDIRSKARTLSHPVTDPKDLPKWNYDGSSTGQAPGKDSEVILYxxxxxxxxxxxxNNILVICDTYTPAGEPIPTNKRANAAKIFSHPDVVVEEPWYGIEQEYTLLQKDVNWPLXXXXXXXXXXXXXXXXXXXADKAYGRDIVDAHYKACLYAGINISGINGEVMPGQWEFQVGPTVGISSGDQVWAARYLLERITEVAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMREEGGIKVIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEREGKGYFEDRRPASNMDPYIVTSMIAETTILLK
A9NSW2_______________MSLLTDLINLNLSDVTEKIIAEYIWIGGSGTDIRSKARTLSHEVSDPKDLPKWNYDGSSTGQAPGKDSEVIIYxxxxxxxxxxxxKNILVICDTYTPAGEPIPTNKRANAAKIFSHPDVVAEEPWFGIEQEYTLLQKDVQWPLGWPIGGYPGPQGPYYCGTGADKAYGRDIVDAHYKACLYAGINISGINGEVMPGQWEFQVGPTVGISSGDQVWAARYILERITEVAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMREEGGIKVIKKAIEKLGLRHKEHISAYGEGNERRLTGRHETADINTFSWGVANRGASIRVGRDTEREGKGYFEDRRPASNMDPYIVTSMITETTILLK

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
1630	2	3	1	92	0.992777
1640	6		93		0.999999
1643	3	88	7		0.998862
1644	7		92		0.999664
1649	91		8		0.999766

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain