SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15348

Length: 1020 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene15348

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Probable glutathione S-transferase n=1 Tax=Picea mariana RepID=O65056_PICMA	-	-	75%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	78%
Blast2go	tau class glutathione s-transferase	-	-	79%

Source	GOs	Term	Type	e value	Identity
Blast2go	transferase activity	GO:0016740	Molecular Function	0.0	79%

Source	InterPros	Term	Type	Identity
Blast2go	Glutathione S-transferase, N-terminal	IPR004045	-	79%
Blast2go	Glutathione S-transferase, C-terminal-like	IPR010987	-	79%
Blast2go	Thioredoxin fold	IPR012335	-	79%
Blast2go	Thioredoxin-like fold	IPR012336	-	79%
Blast2go	Glutathione S-transferase/chloride channel, C-terminal	IPR017933	-	79%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9P2P9

Fln msg: Overlapping hits, possible frame ERROR between 516 and 512,

Fln protein: MEGCGEDGQVKLLGVAYSYFVVRVRIALALKGIHYEFIEEDLQNKSELLLQSNPVHKKIPVLIHNGKPVCESMIIVQYIEEAWDRKAPFLMPRDPYDRAIARFWADFIDDKVIPSILRAFKGQGEEQQKAVEESVANLLLLEEVLRSTGKAYFGGDGIxxxVDIALGGQLVPIQTVEKVTNIVLIDAQKTPLLSAWADQFCKADKVKKALPDRAEIFVLLSARRAKLSITTPGQLERRRLTDS*

Protein Length: 245

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTCATAGCG_-_ATGGAGGGTTGTGGAGAGGATGGGCAAGTAAAGCTGTTAGGTGTGGCTTACAGTTACTTCGTGGTGCGGGTTCGCATAGCACTTGCTCTTAAAGGTATCCATTATGAGTTCATTGAAGAGGACCTTCAAAATAAGAGCGAGCTGCTGCTGCAATCGAACCCTGTTCACAAGAAGATTCCGGTGCTCATACACAACGGGAAGCCTGTTTGTGAATCGATGATTATTGTGCAATATATCGAGGAGGCATGGGACAGGAAAGCGCCATTTCTGATGCCCAGAGATCCATATGACCGAGCCATTGCCCGCTTCTGGGCTGACTTCATAGACGATAAGGTCATTCCAAGTATTCTAAGAGCTTTCAAGGGCCAGGGTGAGGAGCAACAGAAAGCGGTGGAAGAATCGGTGGCAAACCTTCTTTTGTTGGAGGAAGTGTTGCGAAGTACCGGAAAAGCGTATTTTGGAGGAGATGGGATAxxxxxxxxxGTTGACATCGCACTGGGCGGGCAATTGGTACCCATCCAAACAGTCGAGAAGGTTACCAATATAGTTTTAATCGACGCACAAAAAACGCCGCTATTAAGTGCATGGGCTGACCAATTTTGTAAAGCCGACAAAGTGAAAAAAGCGTTGCCTGATCGGGCCGAGATCTTTGTATTACTGTCCGCGAGGAGAGCGAAGCTGAGCATCACCACCCCTGGCCAATTAGAAAGGAGGCGGCTGACAGATAGCTAA___AAATAATATTTATAAGATAGCTAAAATAATATTGGTCTTCTTGTGTGTTGTGTTTCCATGTTGTGAGACACAACGCTGGATTTATGTTATCAGGTGTGTTTATACTCCTGCAGATTGGTGGGTAATAAAGCTGCTGTTTTATACATGTGCATTTAATTTCTTAAATGATATGTATTTTGCTCCAAAGTGTTTTGAATCATGAATATCTTTCAGGGTTCTTACATCCCTAAAAGTATTATTAAATAAATTTTCTG

Fln Alignment:

sp_v1.1_unigene15348___MEGCGEDGQVKLLGVAYSYFVVRVRIALALKGIHYEFIEEDLQNKSELLLQSNPVHKKIPVLIHNGKPVCESMIIVQYIEEAWDRKAPFLMPRDPYDRAIARFWADFIDDKVIPSILRAFKGQGEEQQKAVEESVANLLLLEEVLRSTGKAYFGGDGIxxVDIALGGQLVPIQTVEKVTNIVLIDAQKTPLLSAWADQFCKADKVKKALPDRAEIFVLLSARRAKLSITTPG
A9P2P9_______________MEACGEEGQVKLLGNTRSSFVVRVRIALALKGIHYEFIEENTPTKSQLLLQSNPVHKKIPVLIHNGRPVCESMIIVQYIEETWDTKAPNLMPKDPYDRAIARFWTAFVEDKLVLSILGVFNGHGEQQLKAAEESVANFLLLEEVLQTTGKAYFGGDEIxxVDISLGGILVFTKTLEKVTNIVLIDTDKMPQLSAWVDRFSNADGVKEVLPDRGEIFELITGWRAKLTSPPAG

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
120			16	83	0.994512
123	16	83			0.994512
124		16		83	0.994512
130	83		16		0.994512
132		16	83		0.994512
134	83		16		0.994512
137	14	85			0.993533
138	85	14			0.993533
140		14		85	0.993533
374	85	14			0.993533
494		20		80	0.995493
498			83	16	0.994512
500		16	83		0.994512
503		25	75		0.996473
504	75	25			0.996473
507			75	25	0.996473
510	75	25			0.996473
512	60	20			0.99255
514			60	40	1.0
515		40	60		1.0
516	20		80		0.995493
517		20		80	0.995493
518	20		20	60	0.99255
519		40	60		1.0
520	20	20	60		0.99255
524	80		20		0.995493
528		80	20		0.995493
529			80	20	0.995493
534		20	80		0.995493
535	20		80		0.995493
609	25		75		0.996473
813	12			87	0.992554
838		25	75		1.0
862			12	87	0.992554
910		80	20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain