SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene15299

Length: 1671 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene15299

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Monodehydroascorbate reductase n=1 Tax=Mesembryanthemum crystallinum RepID=Q93YG1_MESCR	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	90%
Blast2go	monodehydroascorbate reductase	-	-	83%

Source	ECs	Term	Type	e value	Identity
Blast2go	Monodehydroascorbate reductase (NADH).	EC:1.6.5.4	-	0.0	83%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Ascorbate and aldarate metabolism	00053		0.0	83%
Blast2go	Metabolic pathways	01100		0.0	83%

Source	GOs	Term	Type	Identity
Blast2go	electron transport chain	GO:0022900	Biological Process	83%
Blast2go	flavin adenine dinucleotide binding	GO:0050660	Molecular Function	83%
Blast2go	disulfide oxidoreductase activity	GO:0015036	Molecular Function	83%
Blast2go	monodehydroascorbate reductase (NADH) activity	GO:0016656	Molecular Function	83%
Blast2go	cytoplasm	GO:0005737	Cellular Component	83%

Source	InterPros	Term	Type	Identity
Blast2go	Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain	IPR001327	-	83%
Blast2go	FAD-dependent pyridine nucleotide-disulphide oxidoreductase	IPR013027	-	83%
Blast2go	FAD/NAD-linked reductase, dimerisation	IPR016156	-	83%
Blast2go	Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain	IPR023753	-	83%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUH7

Fln msg: Overlapping hits, possible frame ERROR between 602 and 595,

Fln protein: MAAKSFKYIILGGGVAAGYAAREFVKLGVKSGELAIISKEAVAPYERPALSKAYLFPQGAARLPSFHVCVGSGGERQLPEWYTEKGIELILSTEVVKAEIATKTLTSAEGTTFKYDTLIIATGSTVINLSDFGTQGADAxxxxFYLREIAEADTLVSAIQSKPEGTAVVVGGGYIGLELAAVLKINKYKVKMVCPEPGFMPRLFTADIASFYEGYYKGKTVDIIKGRATGFSTDEHGNVKMVKLKSGRELEADIVVVGVGARPLTGLFKGQLQEEKGGIKTDGSFRTSVPDVYAVGDVAAFPMKIYGDIRRVEHVDHARKSAAQAVQAIKAKEEGRIVEEYDYLPYFYSRSFDLSWQFYGDNVGESVIFGDNNPATSKAKFGSYWIKDGRVVGVFLESGTPEENKAIAKVARVKPLVESKEELIKDGLAFASKISSAFT*

Protein Length: 441

Fln nts: TTCCTTTAGAAGGAATTGATTCCGAGTCTGACTGCTTCATCGCATCTTCTGCCCAGCCACAGCCGCTACGGTCAACTCTATTCAAGAGTTTTGTTTGCTCTCAATCTTCTTCGACCTTAGCAAACAGTTCTTGAGTTCCCTCGGTTCTGTGCAGATTCATTCGATCAAACATTGCA_-_ATGGCGGCAAAATCTTTCAAGTATATTATTCTGGGAGGTGGCGTTGCAGCTGGTTATGCAGCCAGAGAATTTGTTAAGTTGGGTGTCAAATCTGGAGAGCTCGCAATTATATCAAAAGAGGCTGTAGCTCCATATGAAAGACCTGCACTTAGCAAAGCTTATCTCTTTCCGCAAGGAGCTGCGCGACTTCCGAGCTTCCATGTCTGTGTTGGGAGTGGAGGAGAGAGGCAGCTCCCGGAATGGTATACTGAGAAAGGTATAGAGTTGATTCTCAGCACTGAAGTGGTGAAAGCTGAAATTGCCACCAAAACTCTCACTAGCGCAGAAGGGACTACTTTCAAATATGACACTTTAATCATTGCAACTGGTTCCACGGTTATCAACTTATCTGACTTCGGCACACAAGGAGCTGATGCCxxxxxxxxxxxxTTTTATCTGAGAGAGATTGCTGAAGCTGATACCTTGGTATCTGCAATACAGTCAAAACCAGAAGGCACAGCTGTGGTTGTTGGTGGAGGATATATTGGCCTTGAACTTGCTGCAGTCCTAAAAATCAACAAATATAAAGTTAAAATGGTGTGCCCCGAGCCAGGGTTCATGCCTCGTCTTTTCACTGCAGACATAGCTTCTTTCTATGAGGGCTATTATAAAGGAAAGACTGTGGATATTATCAAGGGAAGGGCCACAGGATTCAGCACTGATGAGCATGGAAATGTTAAGATGGTAAAGCTGAAGAGTGGCAGGGAACTGGAGGCAGATATTGTTGTTGTTGGTGTTGGAGCAAGACCTTTGACAGGCCTTTTCAAAGGTCAGCTTCAGGAGGAAAAGGGTGGCATTAAGACGGATGGTTCCTTCAGAACTAGTGTTCCTGATGTTTATGCGGTAGGAGACGTGGCAGCTTTCCCTATGAAGATTTATGGGGACATTAGAAGAGTCGAACATGTTGATCATGCTAGGAAATCAGCAGCACAAGCCGTACAGGCAATTAAAGCCAAAGAAGAAGGAAGGATCGTTGAAGAATATGACTATCTTCCATATTTTTATTCACGTTCATTTGATTTATCATGGCAATTCTATGGAGACAATGTAGGTGAATCCGTTATTTTCGGTGACAATAATCCTGCGACATCGAAAGCCAAATTTGGATCATATTGGATAAAAGATGGTAGGGTGGTAGGTGTATTCTTGGAGAGTGGTACTCCTGAAGAAAACAAAGCTATTGCTAAGGTAGCACGTGTGAAGCCTTTGGTTGAAAGCAAGGAGGAACTGATAAAGGATGGGCTGGCATTTGCTTCTAAGATATCCTCGGCTTTCACTTAA___ATCGGCTTTCTGTTAATTCATCAGCATGCTGCATTACTGTTGGTCTCTGTTCACCTTGGATATATCTGATTGACTTTTGTTATGAGATATTGGATTTGACTTTTGTTATGAGGCATTGGACTTTTAGTCAGGGTGTAGCTACTTCGTTAGTAAATCAAACTCGCTACATATTTTTGC

Fln Alignment:

sp_v1.1_unigene15299___MAAKSFKYIILGGGVAAGYAAREFVKLGVKSGELAIISKEAVAPYERPALSKAYLFPQGAARLPSFHVCVGSGGERQLPEWYTEKGIELILSTEVVKAEIATKTLTSAEGTTFKYDTLIIATGSTVINLSDFGTQGADAxxxFYLREIAEADTLVSAIQSKPEGTAVVVGGGYIGLELAAVLKINKYKVKMVCPEPGFMPRLFTADIASFYEGYYKGKTVDIIKGR-ATGFSTDEHGNVKMVKLKSGRELEADIVVVGVGARPLTGLFKGQLQEEKGGIKTDGSFRTSVPDVYAVGDVAAFPMKIYGDIRRVEHVDHARKSAAQAVQAIKAKEEGRIVEEYDYLPYFYSRSFDLSWQFYGDNVGESVIFGDNNPATSKAKFGSYWIKDGRVVGVFLESGTPEENKAIAKVARVKPLVESKEELIKDGLAFASKI
A9NUH7_______________MAEKFFKYVIVGGGVAAGYAAREFVKLGLKSGELAIISKEAVAPYERPALSKAYLFPEGAARLPGFHVCVGIGGERQLPEWYSEKGIELILSTEVVKAEIASKTLTTAEGTTFKYDTLIIATGSTVIKLSDFGVQGADAxxxFYLREIAEADALVSAIQSKPEGTAVIVGGGYIGLELAAVLRINKYKVKMVYPEPWCMPRLFTADIAAFYEGYYRGKGVEIIKGTVATGFVTDEHENVKIVKLKDGRELEADIVVVGVGGRPLTGLFKGQLEEEKGGIKTDGFFKTSVPDVYAVGDVATFPMKIYGDIRRVEHVDHARKSAAQAVQAIKAKEEGKSFEEYDYLPYFYSRSFDLSWQFYGDNVGEAIIFGDNNPATPKAKFGSYWIKDGRVVGVFLENGTPEENKAIAKVARLKPLVESKEELIKDGLAFASKI

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
1177		33	66		0.997452
1179	66			33	0.997452
1180	66	33			0.997452
1184		33		66	0.997452
1186			66	33	0.997452
1188			33	66	0.997452
1190	66	33			0.997452
1191	66			33	0.997452
1193	66	33			0.997452
1195		33		66	0.997452
1196	66			33	0.997452
1197		33		66	0.997452
1198	33		66		0.997452
1484	33	66			1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
935	946	TTG TTG TTG TTG	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain