SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene14231

Length: 1917 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene14231

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	tRNA(Ile)-lysidine synthetase; K04075 tRNA(Ile)-lysidine synthase [EC:6.3.4.-]	-	-	37%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	85%
Blast2go	trna -lysidine synthetase	-	-	59%

Source	ECs	Term	Type	e value	Identity
Blast2go	Protein-synthesizing GTPase.	EC:3.6.5.3	-	0.0	59%

Source	ECs	Term	Type	e value	Identity
AutoFact	Ligases, Forming carbon-nitrogen bonds, Other carbon--nitrogen ligases.	EC:6.3.4.-	-	0.0	37%

Source	KEGGs	Term	Identity
AutoFact	Purine metabolism	00230	37%
AutoFact	Metabolic pathways	01100	37%
AutoFact	Biosynthesis of secondary metabolites	01110	37%

Source	GOs	Term	Type	Identity
Blast2go	translation	GO:0006412	Biological Process	59%
Blast2go	tRNA processing	GO:0008033	Biological Process	59%
Blast2go	ligase activity, forming carbon-nitrogen bonds	GO:0016879	Molecular Function	59%
Blast2go	ATP binding	GO:0005524	Molecular Function	59%
Blast2go	cytoplasm	GO:0005737	Cellular Component	59%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LL25

Fln msg:

Fln protein: MGHLWCFCDMQTLGLPFHHAFLQTPTPPLSPLQSPTPQLSPLLFTPKILLSYPKPHPIHKISALAGFSRELKSNSRANTNIRDHDLAQILVKRLARNLSDRHLLQAGERILVAVSGGQDSICLLWMLSQLQQSWEWKLGVVHCDHQWNPQSSAAQASHVAQVAREMGHNFYQSLPTMDAFGETRARAWRYGVFHRIALRHGYDVVITAHTASDRVETMLHNLFRGTGLQGMQSLTWARELPVRIPSHKPYKKVRLVRPLLDTTRTELRKLCETLQLPLWPDPSNFSLEINRNRIRHELLPYLRENFGSGIDRNLARWVEILHGEESFLHSLCENIRSKAEKSMDHGVMKLDVSIVESLPLAVQRRVLKQFIESHTGKSACFDTIERLRLAYGQTSNGNKNVRVSMRGGFEVHLREKMMLLIKKGVQNPQ*

Protein Length: 431

Fln nts: ATGGGGACAGAAACTTTACAGAATTTACTGCCACACGGCTATCTGCATGCGGAAGCTCTAGTGGGCTTTCTGC_-_ATGGGGCATCTTTGGTGCTTCTGTGACATGCAAACGCTTGGCCTGCCATTTCACCATGCCTTCCTTCAAACCCCAACACCACCATTATCACCGCTTCAAAGCCCGACACCACAATTATCACCGCTTCTGTTTACACCCAAAATCCTCTTGTCATACCCCAAACCTCATCCTATTCACAAAATTTCAGCACTTGCAGGGTTTTCTCGGGAGCTCAAATCAAATTCTCGAGCAAATACAAACATAAGAGACCACGACCTCGCTCAAATCTTGGTGAAGCGACTGGCCCGAAATTTATCGGACAGACACCTATTGCAGGCGGGGGAGAGAATCCTGGTGGCCGTTTCAGGTGGGCAGGACTCCATATGCCTTCTGTGGATGCTCTCTCAGTTGCAGCAAAGCTGGGAATGGAAGTTGGGTGTGGTTCACTGCGACCATCAATGGAATCCTCAATCATCAGCAGCACAGGCTTCCCACGTTGCCCAAGTCGCCAGGGAAATGGGCCACAATTTCTATCAGAGCTTGCCCACCATGGACGCCTTCGGCGAAACCAGAGCTCGGGCATGGAGGTATGGGGTTTTTCATAGGATTGCATTACGCCATGGTTATGATGTTGTTATCACTGCACACACTGCAAGTGACAGAGTAGAGACTATGCTTCACAATTTATTCCGAGGGACTGGGCTCCAGGGCATGCAGTCCCTTACTTGGGCTAGGGAGTTGCCGGTTCGAATTCCATCCCACAAACCCTATAAAAAAGTGAGGTTAGTGAGGCCTCTACTCGATACAACTAGGACTGAGCTTAGAAAACTCTGTGAAACCCTGCAGCTTCCACTGTGGCCTGATCCCTCTAATTTTAGCCTTGAGATAAACAGAAACCGCATTCGCCATGAACTGCTGCCATACCTTAGGGAAAATTTTGGAAGTGGGATTGATAGGAATCTTGCTAGGTGGGTAGAAATTCTTCATGGAGAGGAATCATTCTTGCATTCTCTGTGCGAGAACATAAGGTCCAAGGCCGAGAAGTCGATGGATCATGGTGTGATGAAGCTCGATGTTTCCATTGTAGAGTCTCTGCCCTTGGCGGTTCAGCGACGGGTGCTCAAGCAGTTTATTGAGTCGCATACTGGGAAAAGCGCGTGCTTCGATACAATCGAGAGGTTGCGACTTGCCTATGGACAGACTAGTAATGGCAACAAGAATGTTAGGGTGTCTATGCGAGGTGGTTTTGAGGTGCATTTGCGAGAGAAGATGATGCTTCTTATAAAAAAAGGTGTCCAAAATCCTCAGTGA___GGTACTAAAAGGTTATGATAGTAACAGCAAGTTTTATGGTAGTTAACATTTTTATATTGAATTTCGATGGGTTTAATGGCTACGTTGAGAATTCAGGAGACACAGGTCTCGATTCTGCTATTGCTGCAGAGCATAACAAGGGCAGCTATCATTTGGATCTTGTGCTCAGAAGGCGGTAGTGATGATCCAGCCAGCCCTAGATGCTGTTGTATTCTGGTACTTGGGTGCCTACTTCAACTCTGTTCACTATGAATTTGATGGCTTTGAAAAATATGACTTTGTTTCTATCATACTGCACTTTACTCTTCAGTATGGAAAGTATCACATTTGTTTTATCTTATGTCTTATGGGCAAGCATGGATCGTTTGTGTATTCTTGAATTATAAATTTCTACGGATGCAAAAACTTGTGAACCGTGATAGAGGACAAAAATGAAACATCTATTGAAACAAAAATAGCAAGATGATCTTCAACCCACTGGTACTCCAACCTGTATCTTCATTCAAGGCTTACAATGAATCTGCAAAGAGCAGAACCACTCTCGAGTCTTATTC

Fln Alignment:

sp_v1.1_unigene14231___MQTLGLPFHHAFXXXXXXXXXXXXXXXXXXXXXXFTPKILLSYPKPHPIHKISALAGFSRELKSNSRANTNIRDHDLAQILVKRLARNLSDRHLLQAGERILVAVSGGQDSICXXXXXXXXXXXXEWKLGVVHCDHQWNPXXXXXXXXXXXXXXREMGHNFYQSLPTMDAFGETRARAWRYGVFHRIALRHGYDVVITAHTASDRVETMLHNLFRGTGLQGMQSLTWARELPVRIPSHKPYKKVRLVRPLLDTTRTELRKLCETLQLPLWPDPSNFSLEINRNRIRHELLPYLRENFGSGIDRNLARWVEILHGEESFLHSLCENIRSKAEKSMDHGVMKLDVSIVESLPLAVQRRVLKQFIESHTGKSACFDTIERLRLAYGQTSNGNKNVRVSMRGGFEVHLREKMMLLIKKGVQNPQ
B8LL25_______________MQTLGLPLHHVFPQTPTPPFRPLLV----------TPKVLLSNPKPHPLHKISAASGFSQEPKSNSSANANRRDHDIPQILVKRLARNLSDRHLVQAGERILVAVSGGQDSICLLWMLSQLQQSWEWKVGVVHCDHQWNPQSSAAQASHVAHVAREMGYDFYQSLPTMDAFGETRARTWRYGVLHRIALRHAYDVVITAHTASDRIETLLHNLFRGTGLQGMQSLTWTREFRVRVPYHKPYKHVRLVRPLLDTTRTELRKLCETLQLPLWPDPSNFNLEINRNRIRHELLPYLRENFSSGIDRNLARWVEILHGEESFLHSLCENIRSKAETSMDHGVMKLDVSIVESLPLALQRRVLKQFIESHTGKSACFDTIEKLRLAYGHTSNGNKRVRVSLRGGFEVHLQEKMILLLRKDVPNAQ

SNPs (tot: 83)

Position	A %	C %	G %	T %	Probability
118		83		16	0.994512
123	66		33		1.0
124		33		66	1.0
147		83	16		0.994512
152	16	83			0.994512
170			30	70	1.0
174	70			30	1.0
175	70		30		1.0
177		30	70		1.0
179		70		30	1.0
180		70		30	1.0
181	20		80		0.999996
182	70		30		1.0
185	70		30		1.0
186		70		30	1.0
190	50	20			1.0
191	70			30	1.0
192		30		70	1.0
193	30			70	1.0
202			20	80	1.0
207		6		80	0.999961
209			13	86	0.999973
212	80	13	6		0.999961
213		86		13	0.999973
214		80		20	1.0
215		80	20		1.0
216	80		13	6	0.999961
217	80	20			1.0
218	86			13	0.999973
219	80	6	13		0.999961
233		60		40	1.0
287	46		53		1.0
312	58		41		1.0
369		13		86	0.999973
374	73		26		1.0
543		10	90		0.90298
550	90		10		0.90298
579		80		20	0.995493
617	25	50			0.995533
643			75	25	0.996473
666			80	20	0.995493
704		80		20	0.995493
715	80		20		0.995493
729	75			25	0.996473
771	25	75			0.996473
942		16	83		0.994512
943	83	16			0.994512
945	83			16	0.994512
946	83		16		0.994512
947	83		16		0.994512
949	83			16	0.994512
951	83			16	0.994512
952	83	16			0.994512
960	16			66	0.988813
973		83		16	0.994512
978	83	16			0.994512
1018		83	16		0.994512
1019			16	83	0.994512
1020			16	83	0.994512
1180	85		14		0.993533
1201	85		14		0.993533
1288			12	87	0.999964
1291	87		12		0.999964
1298		12	87		0.999964
1403		10		90	0.90298
1404	90		10		0.90298
1405		10		90	0.90298
1406	10		90		0.90298
1412			10	90	0.90298
1416	70		20		0.999998
1417	20			80	0.999996
1418			10	90	0.90298
1419			10	90	0.90298
1421		10		90	0.90298
1423		10		90	0.90298
1424	90		10		0.90298
1425	10			90	0.90298
1426		10		90	0.90298
1427			70	30	1.0
1469		80	20		1.0
1471		80	20		1.0
1532			89	10	1.0
1543	16		83		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain