SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene14225

Length: 1025 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene14225

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Chloroplast stromal ascorbate peroxidase n=1 Tax=Solanum lycopersicum RepID=D2SLU7_SOLLC	-	-	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	85%
Blast2go	thylakoid-bound ascorbate peroxidase	-	-	79%

Source	ECs	Term	Type	e value	Identity
Blast2go	L-ascorbate peroxidase.	EC:1.11.1.11	-	0.0	79%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Ascorbate and aldarate metabolism	00053		0.0	79%
Blast2go	Glutathione metabolism	00480		0.0	79%

Source	GOs	Term	Type	Identity
Blast2go	response to oxidative stress	GO:0006979	Biological Process	79%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	79%
Blast2go	heme binding	GO:0020037	Molecular Function	79%
Blast2go	L-ascorbate peroxidase activity	GO:0016688	Molecular Function	79%
Blast2go	chloroplast stromal thylakoid	GO:0009533	Cellular Component	79%
Blast2go	chloroplast thylakoid membrane	GO:0009535	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Blast2go	Haem peroxidase, plant/fungal/bacterial	IPR002016	-	79%
Blast2go	Plant ascorbate peroxidase	IPR002207	-	79%
Blast2go	Haem peroxidase	IPR010255	-	79%
Blast2go	Peroxidases heam-ligand binding site	IPR019793	-	79%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9P0R1

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 18 aas, your sequence is shorter than subject: 268 - 334

Fln protein: MAMALGFAAGSSLTLQHNSTRFPKISTSRSSRGRCRVGYVSTVICFASDPQQLKQARQDLNDLIKTTRCNPLLIRLGWHDAGTYDKNIQEWPKCGGANGSLHFEIELNHKANAGLVNGLKLLQPIKDKYPNITYADLFQLASATAIEDAGGPEIPMKYGRLDAPRPEDCPPEGKLPDAGPPSPAAHLRDIFYRMGLTDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTVEWLKFDNSYFKEIKKKGSRSAGITN*

Protein Length: 269

Fln nts: CGTCGTGACCTTGGCTGTGCAAAATTCTCCCCTGCTGGAGCAGCTTATCAACAAGCTGCGAATCCTTTTGTGGAAAATCA_-_ATGGCTATGGCTTTGGGGTTTGCAGCAGGTTCCTCTCTGACCCTCCAGCACAACTCCACACGTTTTCCGAAGATATCAACTAGCAGAAGCAGCAGGGGAAGGTGCCGAGTAGGCTATGTGTCTACAGTCATTTGCTTTGCATCCGATCCTCAGCAACTCAAACAGGCCAGACAGGACCTGAACGACCTCATTAAAACCACTCGCTGTAATCCACTTCTAATTCGATTGGGATGGCATGATGCTGGAACATATGACAAGAACATTCAAGAGTGGCCAAAGTGTGGTGGAGCAAATGGGAGCTTGCACTTTGAGATAGAACTAAACCACAAAGCAAATGCTGGTCTTGTAAATGGATTGAAGCTCTTACAGCCCATCAAAGACAAGTATCCAAATATTACCTATGCAGATTTGTTCCAGTTAGCCAGCGCCACAGCTATTGAGGATGCTGGAGGCCCTGAAATTCCTATGAAATATGGGCGACTGGATGCACCTAGACCTGAAGATTGTCCACCAGAAGGAAAGCTCCCAGATGCTGGTCCTCCTTCACCAGCTGCTCATCTGCGTGATATCTTCTACAGAATGGGGTTGACAGATAAGGAAATTGTTGCATTATCAGGTGCGCACACTCTTGGAAGATCCAGGCCAGATCGTAGTGGCTGGGGCAAGCCAGAAACCAAATACACAAAAGATGGTCCAGGAGCACCAGGAGGTCAATCCTGGACAGTAGAGTGGCTGAAATTTGATAATTCTTACTTCAAGGAGATAAAGAAAAAGGGATCAAGATCTGCTGGTATTACCAACTGATGCTGTTCTTTATGGTATGATTCCTTCAAGCTACATGCGCAAAATCTGAGGCGTTCATCCTCCGCTCTCTTGCTTCTCTTCACCACCTGATGAACCACTAGTGCAGGTATCGAAGCCCCTACAACTCCCCACCACCACTCC

Fln Alignment:

sp_v1.1_unigene14225___MAMALGFAAGSSLTLQHNSTRFPKISTSRSSRGRCRVGYVSTVICFASDPQQLKQARQDLNDLIKTTRCNPLLIRLGWHDAGTYDKNIQEWPKCGGANGSLHFEIELNHKANAGLVNGLKLLQPIKDKYPNITYADLFQLASATAIEDAGGPEIPMKYGRLDAPRPEDCPPEGKLPDAGPPSPAAHLRDIFYRMGLTDKEIVALSGAHTLGRSRPDRSGWGKPETKYTKDGPGAPGGQSWTVEWLKFDNSYFKEIKKKGSRSAGITN*CCSLWYD-SFKLHAQ
A9P0R1_______________MALALGFAAASSLFLPHNFTRFPKISTSSSSRGRCRVRHVSTVICFASDPQQLKQARQDLNDLIKTTRCNPLLIRVGWHDAGTYDKNIQEWPKRGGANGSLHFEIELSHKANAGLVNALKLLRPIKDKYPNITYADLFQLASATAIEEAGGPVIPMKYGRLDAPKPEDCPPEGKLPDAGPPSPAAHLRDVFYRMGLTDKEIVALSGAHTLGRSRPERSGWGKPETKYTKDGPGAPGGQSWTVEWLKFDNSYFKEIKEQRDQDLLVLPTDAVLFEDPGFKVYAE

SNPs (tot: 64)

Position	A %	C %	G %	T %	Probability
710		80		20	0.995493
844	66			33	0.997452
851	33		66		0.997452
852	66		33		0.997452
853	66		33		0.997452
857	66			33	0.997452
858	66			33	0.997452
859	33		66		0.997452
860	33		66		0.997452
866	66		33		0.997452
867	33		66		0.997452
869			33	66	0.997452
870		66	33		0.997452
871	33			66	0.997452
872	33		66		0.997452
873	33	66			0.997452
874			33	66	0.997452
875		33	66		0.997452
876	33		66		0.997452
878	66	33			0.997452
879	33			66	0.997452
880			33	66	0.997452
881	66			33	0.997452
882		66	33		0.997452
883	33	66			0.997452
884	66	33			0.997452
886	33	66			0.997452
887	33			66	0.997452
888		33	66		0.997452
889	66		33		0.997452
890		33		66	0.997452
892		66	33		0.997452
893		33		66	0.997452
895			33	66	0.997452
896		33		66	0.997452
897	33	66			0.997452
898	33			66	0.997452
899		33		66	0.997452
900	33			66	0.997452
901	33		33	33	0.997753
902	33			66	0.997452
903		33	66		0.997452
904	33		66		0.997452
905	33			66	0.997452
907	66			33	0.997452
908		33		66	0.997452
909		33	66		0.997452
910	66	33			0.997452
911	33			66	0.997452
912			33	66	0.997452
913		33	33	33	0.997753
914		66		33	0.997452
915		33		66	0.997452
916	33			66	0.997452
917	33	66			0.997452
918	33		33	33	0.997753
919	33	33	33		0.997753
920		33	66		0.997452
921		66	33		0.997452
922			33	66	0.997452
923	66			33	0.997452
924		66		33	0.997452
925	66	33			0.997452
926			33	66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain