SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene13784

Length: 3041 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene13784
CGTCGTGACCTTGGCTGTCACTCAGACCCAGGGCTTCTGAAAATTCGGTAGAAGATATACAGTCTGCCGCTGCTGCCACAATTTCCTTTGCAGTGCAATTCAGAGCACCTGTAAGTGTGGGCGATCTCAATGTATGTGCCAGAGTGTGGCAGAAAAACAGTGTGTTGACTCTAGATGATGCATTGGGTAGATTGCAGGATTCAAAGTCCTTATTCATGGCTTCCCATGTGGTTGGTTATCCCCGAATTGCCCCCAAGAGAGAACTCAAGTTTGCACTTGAATCCTTTTGGGATGGCAAGAGCAGTGCAACTGAGTTGCAGAATGTAGCAAAGAATTTGAGAGCTGCAACATGGAAACAAATGGCTGATGCTGGTCTAACATACATCCCAAGCAACACCTTTTCCTACTATGATCAGGTCCTTGACACAACTGCCATGGTTGGGGCTGTTCCTCCACGATATGGGTGGACTGGTGGTGAGATTGGATTCGACACTTACTTCTCAATGGCACGTGGAAATGCTAAGCTTCCTGCTATGGAGATGACCAAGTGGTTTGACACTAACTACCACTACATCGTTCCAGAGCTGGGACCAGAAATTAAATTCAAGTACTCTTCACACAAAGCTGTGGATGAATACAAAGAGGCTAAAGAGATTGGTATTGAGACTGTACCTGTCATTATAGGTCCTGTGTCATACTTGTTGTTATCAAAGCCTGCAAAGGGTGTTCCTAAAACATTTGACCTTCTTTCTCTGCTTGATTCGATTCTGCCGGTCTACAAGGAGGTCTTAGGTGAGTTGAAGGAGGCTGGGGCAACATGGATACAGTTTGATGAACCCTTGCTTGTAATGGACCTTGAGGCTCACCACCTGAATGCTTTCTGTAAAGCTTATTCTTTCTTAGAAGAAGTGTTTGTTGGGACCAAATTGCTTGTAGAAACATACTTTGCTGATATTCCTGTTGAGACATATAGGACCATCATTCAGTTGAAGGCTATATCTGCTATTGGGTTGGACCTAGTACGTGGAAGTAAAACACTCAACCTTGTCAAAACTTTTGGCTTTCCTGGAGACAAATACCTTTTTGCTGGAGTAATTGATGGTAGAAACATCTGGGCTAATGATTTGGCAGCATCGTTTAGCATTTTAAAGGATCTCGAAAAGAGTGTTGTTAAAGACAAAATAGTTGTCTCCACCTCCTGCTCTCTTCTCCATACAGCTGTGGATTTAGTAAATGAGACAAAGCTTGATAATGAGATTAAGTCCTGGCTTGCATTTGCTGCACAAAAAGTACTTGAAGTAGTAGCTCTGGCAAAAGCATTGATTGGTCAAGCAGATCGGGCATATTTTGCTGCCAATGCAGCTGCTATATCTTCAAGAAAGACCTCTCCTAGAGTGAACAATAAGGCAGTGCAAAAAGCTGCTGAAGCATTAAAAGGCTCTGATCACCGGCGAGCTACACCAGTAGTTGTTCGTTTAGATAAGCAGCAGAAAAAGTTGAACCTCCCAATTCTTCCAACGACCACAATTGGGTCCTTTCCTCAGACAGTAGAACTTCGCAGAGTTCGCCGTGAATACAAATCAAATAAGATTTCAGAAGCGGATTACATTCAGTCCATTCAGCAGGAAATTGCAAAAGTGGTTAAACTGCAGGAAGATCTGGATATCGATGTTCTAGTGCATGGTGAACCTGAGAGAAACGACATGGTAGAATATTTTGGGGAACAGCTTCAGGGATTTGCATTCACAGTTAATGGCTGGGTACAGTCTTATGGTTCTCGTTGTGTGAAGCCACCGATTATTTATGGAGATGTCAGCCGCCCGAAACCAATGACTGTGTTTTGGTCAAAGTATGCCCAGAGTTTGACTGCCCGCCCTATGAAGGGAATGCTTACAGGTCCTGTGACAATTTTGAATTGGTCCTTTGTAAGAGATGATCAGCCCAGGTCAGAAACCAGCATGCAAATTGCACTAGCTATCAAAGACGAGGTTGAAGATTTGGAGGCAGCTGGTATTCAGGTGATCCAAATCGATGAGGCAGCCCTGAGGGAAGGATTACCTTTGCGTAAGGCAGAACAGGCTCACTATCTGGAATGGGCTGTTCATGGTTTCCGTATCACCAACTGTGGTGTAAAGGATACTACTCAGATCCACACCCACATGTGTTACTCCAACTTCAACGACATTATTCATTCCATTATTGATATGGATGCCGATGTCATTACCATTGAGAATTCCCGCTCCGATGAGAAATTACTCAGAGTTTTCCAGGAGGGTGTGAAATATGGGGCCGGAATCGGCCCTGGGGTTTATGATATACACTCTCCTCGCATTCCAGACACAGAAGAAATTGCAGACCGTATCCGCAAGTTACTCGCTGTGTTGGAGACAAACATCCTGTGGGTAAATCCAGATTGTGGTTTGAAAACCAGGAAGTATGATGAAGTTGTTCCTGCGTTGACCAACATGGTTGCATCTGCCAAGTTGCTGCGAACTGAGCTTGCCTCTGCAAAATGATCAAGAAAGCAATTCAACATCAGCATTTTAGTGCAATAAAGAGGTGGAAGAATAATTTTGGATTACTGAATAAGGGACATATTACGGCCTCCTGTGAAGGGTTAGTTGGTTTGTGGTCTAGCGGTAGATCAGTGGAAGTTCACCATAACTGCCCAAGGTTTGATTCTCAGTGCATATCATATGGCTGTACCTGAGAATCACCGAAGTGACACATCCCAGAGATTGAGCCCACTCTGGCCAAAAATGGAGAGTTTCTGTTAAAAGGAAAAGCCTTAATGTTGATGTAAAGAGACACTAGTTTTTAAAGAATTTTCAGAGTTGTCAGTTGACTGGCCTGATCATTCTTCTAGATATTTCAGTAGACATTTTTCTTCCTGCTATTACAAAAGCATCATGCGCATGGAAAATGTCTCATCTAAAGTTGTTATAACTTATAACATATGGAGTTTCTCATTTCTGCATGCCTGTTGTGCCTACTATTGATATAAAAATTTAAAAATTTTCAGCATGGTTTCTGAGTTTGTT

Ace file of the UniGene sp_v1.1_unigene13784 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Vitamin-b12 independent methionine synthase, 5-methyltetrahydropteroyltriglutamate-homocysteine n=3 Tax=Malpighiales RepID=B9I615_POPTR	-	-	79%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	vitamin-b12 independent methionine 5-methyltetrahydropteroyltriglutamate-homocysteine	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase.	EC:2.1.1.14	-	0.0	89%

Source	KEGGs	Term	Identity
Blast2go	Cysteine and methionine metabolism	00270	89%
Blast2go	Selenocompound metabolism	00450	89%
Blast2go	Biosynthesis of plant hormones	01070	89%
Blast2go	Metabolic pathways	01100	89%
Blast2go	Biosynthesis of secondary metabolites	01110	89%

Source	GOs	Term	Type	Identity
Blast2go	methionine biosynthetic process	GO:0009086	Biological Process	89%
Blast2go	zinc ion binding	GO:0008270	Molecular Function	89%
Blast2go	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity	GO:0003871	Molecular Function	89%
Blast2go	cytoplasm	GO:0005737	Cellular Component	89%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKF4

Fln msg:

Fln protein: MASHVVGYPRIAPKRELKFALESFWDGKSSATELQNVAKNLRAATWKQMADAGLTYIPSNTFSYYDQVLDTTAMVGAVPPRYGWTGGEIGFDTYFSMARGNAKLPAMEMTKWFDTNYHYIVPELGPEIKFKYSSHKAVDEYKEAKEIGIETVPVIIGPVSYLLLSKPAKGVPKTFDLLSLLDSILPVYKEVLGELKEAGATWIQFDEPLLVMDLEAHHLNAFCKAYSFLEEVFVGTKLLVETYFADIPVETYRTIIQLKAISAIGLDLVRGSKTLNLVKTFGFPGDKYLFAGVIDGRNIWANDLAASFSILKDLEKSVVKDKIVVSTSCSLLHTAVDLVNETKLDNEIKSWLAFAAQKVLEVVALAKALIGQADRAYFAANAAAISSRKTSPRVNNKAVQKAAEALKGSDHRRATPVVVRLDKQQKKLNLPILPTTTIGSFPQTVELRRVRREYKSNKISEADYIQSIQQEIAKVVKLQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSKYAQSLTARPMKGMLTGPVTILNWSFVRDDQPRSETSMQIALAIKDEVEDLEAAGIQVIQIDEAALREGLPLRKAEQAHYLEWAVHGFRITNCGVKDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLRVFQEGVKYGAGIGPGVYDIHSPRIPDTEEIADRIRKLLAVLETNILWVNPDCGLKTRKYDEVVPALTNMVASAKLLRTELASAK*

Protein Length: 768

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGACCCAGGGCTTCTGAAAATTCGGTAGAAGATATACAGTCTGCCGCTGCTGCCACAATTTCCTTTGCAGTGCAATTCAGAGCACCTGTAAGTGTGGGCGATCTCAATGTATGTGCCAGAGTGTGGCAGAAAAACAGTGTGTTGACTCTAGATGATGCATTGGGTAGATTGCAGGATTCAAAGTCCTTATTC_-_ATGGCTTCCCATGTGGTTGGTTATCCCCGAATTGCCCCCAAGAGAGAACTCAAGTTTGCACTTGAATCCTTTTGGGATGGCAAGAGCAGTGCAACTGAGTTGCAGAATGTAGCAAAGAATTTGAGAGCTGCAACATGGAAACAAATGGCTGATGCTGGTCTAACATACATCCCAAGCAACACCTTTTCCTACTATGATCAGGTCCTTGACACAACTGCCATGGTTGGGGCTGTTCCTCCACGATATGGGTGGACTGGTGGTGAGATTGGATTCGACACTTACTTCTCAATGGCACGTGGAAATGCTAAGCTTCCTGCTATGGAGATGACCAAGTGGTTTGACACTAACTACCACTACATCGTTCCAGAGCTGGGACCAGAAATTAAATTCAAGTACTCTTCACACAAAGCTGTGGATGAATACAAAGAGGCTAAAGAGATTGGTATTGAGACTGTACCTGTCATTATAGGTCCTGTGTCATACTTGTTGTTATCAAAGCCTGCAAAGGGTGTTCCTAAAACATTTGACCTTCTTTCTCTGCTTGATTCGATTCTGCCGGTCTACAAGGAGGTCTTAGGTGAGTTGAAGGAGGCTGGGGCAACATGGATACAGTTTGATGAACCCTTGCTTGTAATGGACCTTGAGGCTCACCACCTGAATGCTTTCTGTAAAGCTTATTCTTTCTTAGAAGAAGTGTTTGTTGGGACCAAATTGCTTGTAGAAACATACTTTGCTGATATTCCTGTTGAGACATATAGGACCATCATTCAGTTGAAGGCTATATCTGCTATTGGGTTGGACCTAGTACGTGGAAGTAAAACACTCAACCTTGTCAAAACTTTTGGCTTTCCTGGAGACAAATACCTTTTTGCTGGAGTAATTGATGGTAGAAACATCTGGGCTAATGATTTGGCAGCATCGTTTAGCATTTTAAAGGATCTCGAAAAGAGTGTTGTTAAAGACAAAATAGTTGTCTCCACCTCCTGCTCTCTTCTCCATACAGCTGTGGATTTAGTAAATGAGACAAAGCTTGATAATGAGATTAAGTCCTGGCTTGCATTTGCTGCACAAAAAGTACTTGAAGTAGTAGCTCTGGCAAAAGCATTGATTGGTCAAGCAGATCGGGCATATTTTGCTGCCAATGCAGCTGCTATATCTTCAAGAAAGACCTCTCCTAGAGTGAACAATAAGGCAGTGCAAAAAGCTGCTGAAGCATTAAAAGGCTCTGATCACCGGCGAGCTACACCAGTAGTTGTTCGTTTAGATAAGCAGCAGAAAAAGTTGAACCTCCCAATTCTTCCAACGACCACAATTGGGTCCTTTCCTCAGACAGTAGAACTTCGCAGAGTTCGCCGTGAATACAAATCAAATAAGATTTCAGAAGCGGATTACATTCAGTCCATTCAGCAGGAAATTGCAAAAGTGGTTAAACTGCAGGAAGATCTGGATATCGATGTTCTAGTGCATGGTGAACCTGAGAGAAACGACATGGTAGAATATTTTGGGGAACAGCTTCAGGGATTTGCATTCACAGTTAATGGCTGGGTACAGTCTTATGGTTCTCGTTGTGTGAAGCCACCGATTATTTATGGAGATGTCAGCCGCCCGAAACCAATGACTGTGTTTTGGTCAAAGTATGCCCAGAGTTTGACTGCCCGCCCTATGAAGGGAATGCTTACAGGTCCTGTGACAATTTTGAATTGGTCCTTTGTAAGAGATGATCAGCCCAGGTCAGAAACCAGCATGCAAATTGCACTAGCTATCAAAGACGAGGTTGAAGATTTGGAGGCAGCTGGTATTCAGGTGATCCAAATCGATGAGGCAGCCCTGAGGGAAGGATTACCTTTGCGTAAGGCAGAACAGGCTCACTATCTGGAATGGGCTGTTCATGGTTTCCGTATCACCAACTGTGGTGTAAAGGATACTACTCAGATCCACACCCACATGTGTTACTCCAACTTCAACGACATTATTCATTCCATTATTGATATGGATGCCGATGTCATTACCATTGAGAATTCCCGCTCCGATGAGAAATTACTCAGAGTTTTCCAGGAGGGTGTGAAATATGGGGCCGGAATCGGCCCTGGGGTTTATGATATACACTCTCCTCGCATTCCAGACACAGAAGAAATTGCAGACCGTATCCGCAAGTTACTCGCTGTGTTGGAGACAAACATCCTGTGGGTAAATCCAGATTGTGGTTTGAAAACCAGGAAGTATGATGAAGTTGTTCCTGCGTTGACCAACATGGTTGCATCTGCCAAGTTGCTGCGAACTGAGCTTGCCTCTGCAAAATGA___TCAAGAAAGCAATTCAACATCAGCATTTTAGTGCAATAAAGAGGTGGAAGAATAATTTTGGATTACTGAATAAGGGACATATTACGGCCTCCTGTGAAGGGTTAGTTGGTTTGTGGTCTAGCGGTAGATCAGTGGAAGTTCACCATAACTGCCCAAGGTTTGATTCTCAGTGCATATCATATGGCTGTACCTGAGAATCACCGAAGTGACACATCCCAGAGATTGAGCCCACTCTGGCCAAAAATGGAGAGTTTCTGTTAAAAGGAAAAGCCTTAATGTTGATGTAAAGAGACACTAGTTTTTAAAGAATTTTCAGAGTTGTCAGTTGACTGGCCTGATCATTCTTCTAGATATTTCAGTAGACATTTTTCTTCCTGCTATTACAAAAGCATCATGCGCATGGAAAATGTCTCATCTAAAGTTGTTATAACTTATAACATATGGAGTTTCTCATTTCTGCATGCCTGTTGTGCCTACTATTGATATAAAAATTTAAAAATTTTCAGCATGGTTTCTGAGTTTGTT

Fln Alignment:

sp_v1.1_unigene13784___MASHVVGYPRIAPKRELKFALESFWDGKSSATELQNVAKNLRAATWKQMADAGLTYIPSNTFSYYDQVLDTTAMVGAVPPRYGWTGGEIGFDTYFSMARGNAKLPAMEMTKWFDTNYHYIVPELGPEIKFKYSSHKAVDEYKEAKEIGIETVPVIIGPVSYLLLSKPAKGVPKTFDLLSLLDSILPVYKEVLGELKEAGATWIQFDEPLLVMDLEAHHLNAFCKAYSFLEEVFVGTKLLVETYFADIPVETYRTIIQLKAISAIGLDLVRGSKTLNLVKTFGFPGDKYLFAGVIDGRNIWANDLAASFSILKDLEKSVVKDKIVVSTSCSLLHTAVDLVNETKLDNEIKSWLAFAAQKVLEVVALAKALIGQXXXXXXXXXXXAISSRKTSPRVNNKAVQKAAEALKGSDHRRATPVVVRLDKQQKKLNLPILPTTTIGSFPQTVELRRVRREYKSNKISEADYIQSIQQEIAKVVKLQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSKYAQSLTARPMKGMLTGPVTILNWSFVRDDQPRSETSMQIALAIKDEVEDLEAAGIQVIQIDEAALREGLPLRKAEQAHYLEWAVHGFRITNCGVKDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLRVFQEGVKYGAGIGPGVYDIHSPRIPDTEEIADRIRKLLAVLETNILWVNPDCGLKTRKYDEVVPALTNMVASAKLLRTELASAK
B8LKF4_______________MASHVVGYPRIAPKRELKFALESFWDGKSSAAELQNVAKDLRVATWKQMADAGLTYIPSNTFSYYDQVLDTTAMVGAVPPRYGWTGGEIGFDTYFSMARGNAKLPAMEMTKWFDTNYHYIVPELGPEIKFKYSSHKAVDEYKEAKKIGIETVPVIIGPVSYLLLSKPAKGVPKTFDLLSLLDAILPVYKEVLGELKQAGATWIQFDEPALVMDLEAHHLDAFSKAYSFLEEVLVGIKLLVETYFADIPVETYRTIIQLKAISAIGLDLIRGTKTLSLVKTVGFPGDKYLFAGVIDGRNIWANDLAASFSILKDLETIVGKGKIVVSTSCSLLHTAVDLVNETKLDNEIKSWLAFAAQKVLEVVALAKALIGQADQPYFAANAAAISSRKTSPRVNNEAVQKAAEALKGSDHRRATPVIARLDEQQKKLNLPILPTTTIGSFPQTVELRRVRREYKSNKISEADYIQSIQQEIAKVVKMQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPVIYGDVSRPKPMTVFWSKYAQSLTARPMKGMLTGPVTILNWSFVRDDQPRSETSMQIALAIKEEVEDLEASGIQVIQIDEAALREGLPLRKAEQAHYLEWAVHGFRITNCGVKDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLRVFQEGVKYGAGIGPGVYDIHSPRIPDTEEIADRIRKILAVLETNILWVNPDCGLKTRKYNEVVPALTNMVASAKLLRTELASAK

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
2062			14	85	0.994477
2068		85		14	0.994477
2092	83			16	0.995451
2128	20	80			0.995493
2130	80		20		0.995493
2131		80		20	0.995493
2159		20	80		0.995493
2167	80		20		0.995493
2198	80			20	0.995493
2201	60		20		0.99255
2204			80	20	0.995493
2207	20			80	0.995493
2210			20	80	0.995493
2212	80	20			0.995493
2216			20	80	0.995493
2219	80	20			0.995493
2220	20			80	0.995493
2222		75	25		0.996473
2224		25		75	0.996473
2225			75	25	0.996473
2226			33	66	0.997452
2227	33			66	0.997452
2229		66		33	0.997452
2230		66	33		0.997452
2231	33	66			0.997452
2237	25			75	0.996473
2238	25	75			0.996473
2239		75	25		0.996473
2240			20	80	0.995493
2247		80	20		0.995493
2250		80	20		0.995493
2260		25		75	0.996473
2263	75		25		0.996473
2278		25		75	0.996473
2297	75		25		0.996473
2301		25		75	0.996473
2302	25		75		0.996473
2320		25		75	0.996473
2361		80		20	0.995493
2362	20		80		0.995493
2412		20	80		0.995493
2455	66	33			0.997452
2646		25		75	0.996473
2671	25		75		0.996473
2686		75		25	0.996473
2723		20		80	0.995493
2792		66	33		0.997452
2793	66	33			0.997452
2795	33			66	0.997452
2799		66	33		0.997452
2847			22	77	0.999999
3012	16			83	0.994512
3018		42		57	1.0
3019		42	57		1.0
3020	50	50			1.0
3021	50		50		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain