SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene13581

Length: 1762 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene13581

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	HMA2; HMA2; cadmium-transporting ATPase; K01534 Cd2+/Zn2+-exporting ATPase [EC:3.6.3.3 3.6.3.5]	-	-	40%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	31%
Blast2go	cadmium-transporting atpase-like protein	-	-	63%

Source	ECs	Term	Type	e value	Identity
AutoFact	Cadmium-exporting ATPase.	EC:3.6.3.3	-	0.0	40%
AutoFact	Zinc-exporting ATPase.	EC:3.6.3.5	-	0.0	40%

Source	GOs	Term	Type	Identity
Blast2go	cellular biosynthetic process	GO:0044249	Biological Process	63%
Blast2go	zinc ion transport	GO:0006829	Biological Process	63%
Blast2go	cadmium ion transport	GO:0015691	Biological Process	63%
Blast2go	zinc ion transmembrane transporter activity	GO:0005385	Molecular Function	63%
Blast2go	ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism	GO:0015662	Molecular Function	63%
Blast2go	protein binding	GO:0005515	Molecular Function	63%
Blast2go	nucleotide binding	GO:0000166	Molecular Function	63%
Blast2go	cadmium ion transmembrane transporter activity	GO:0015086	Molecular Function	63%
Blast2go	membrane	GO:0016020	Cellular Component	63%

Source	InterPros	Term	Type	Identity
Blast2go	Cof protein	IPR000150	-	63%
Blast2go	ATPase, P-type, H+ transporting proton pump	IPR000695	-	63%
Blast2go	ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter	IPR001757	-	63%
Blast2go	Haloacid dehalogenase-like hydrolase	IPR005834	-	63%
Blast2go	ATPase, P-type, heavy metal translocating	IPR006416	-	63%
Blast2go	ATPase, P-type, ATPase-associated domain	IPR008250	-	63%
Blast2go	ATPase, P-type phosphorylation site	IPR018303	-	63%
Blast2go	HAD-like domain	IPR023214	-	63%
Blast2go	ATPase, P-type, cytoplasmic domain N	IPR023299	-	63%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQ20

Fln msg: STOP codon was not found. Distance to subject end: 158 aas, your sequence is shorter than subject: 587 - 998

Fln protein: KLVEEAQSQRSSTEEFVQKFAKYYTPVIVLTAMAMTLAPLIDHSLGFRKWVYKALVVLVTACPCALVISTPVATACGLAHAAKTGLLIKGGRYLEALGKLKAIAFDKTGTLTEGIFRVVDVKFVDSETIDEHKWLSWIASLESRSSHPMSVAIVSYCRQKGLEPSAEVKDFDILEGEGICGVVDNHKVYIGNVRLSNRLGWTEYVPDTWDIRGATVGYFGTDEKFVGAFSVADNSRPEAAEALDKLKKLGIQLAMLTGDTAASAALIETQVGQIQVYSQLLPQDKVRIIGDLKSVGKTAMIGDGINDAPALAAADIGIAMGVAGSAVAMETSDIALMSNDIRKIAGAIKIGRRTLRKIYINVAISIATKVVIFALTAVGLVYLWMAVLTDVGTGMLVTFNSMLLLKGAKRYGHVVCNRQDCDDGGHCHKDNDGDVYYHVHGGGNRHQVHRNIETSTSADCTHTVQEADTCGVVKCSACNFPSSIHHGCAKPRTSRCCQNSYPPQVDLCASGNCRGGKRQLGSSHINESRFEQRCSSSNNCCKSDKLGNSQMISGTSSSNCCNSSKQTGECTQSNAYEATIEELLVRK

Protein Length: 588

Fln nts: AAGCTTGTGGAGGAAGCACAGAGTCAACGCTCCTCCACCGAAGAGTTCGTTCAAAAATTTGCAAAATATTACACTCCAGTTATTGTATTGACAGCAATGGCGATGACATTGGCACCTTTGATTGACCACTCGCTTGGATTTCGCAAATGGGTTTACAAAGCCTTAGTTGTTCTGGTGACTGCTTGCCCATGTGCATTGGTTATCTCAACTCCAGTGGCCACAGCTTGTGGGTTAGCCCATGCTGCTAAAACAGGCCTCCTCATCAAAGGTGGGAGGTATCTGGAAGCCTTGGGGAAGCTTAAGGCGATAGCGTTTGATAAAACTGGAACACTCACAGAAGGAATATTCAGGGTGGTAGATGTAAAGTTTGTTGATTCCGAAACCATTGATGAGCACAAGTGGCTATCTTGGATAGCAAGCCTTGAAAGTAGATCAAGTCATCCCATGTCCGTTGCAATTGTATCATACTGTCGACAGAAAGGCCTAGAGCCATCAGCAGAGGTGAAGGATTTCGATATACTAGAAGGAGAAGGCATCTGCGGAGTGGTGGACAACCATAAAGTTTACATTGGCAATGTTCGTTTATCAAATCGGTTAGGTTGGACCGAATATGTACCAGACACCTGGGATATCCGAGGAGCAACAGTCGGATACTTTGGGACAGACGAAAAATTTGTGGGGGCCTTCAGCGTGGCCGACAACAGCCGGCCGGAAGCGGCAGAAGCTCTTGATAAGCTCAAGAAATTAGGAATTCAACTCGCTATGCTCACCGGGGATACAGCCGCCTCAGCTGCTCTCATTGAAACACAGGTAGGGCAGATTCAAGTTTATTCTCAACTACTGCCACAGGACAAGGTCCGAATTATTGGAGATTTAAAGTCTGTGGGCAAGACAGCCATGATAGGCGATGGAATCAACGATGCCCCAGCTTTAGCTGCAGCTGACATCGGCATAGCTATGGGTGTAGCAGGTTCGGCCGTGGCCATGGAGACTTCAGATATAGCTCTCATGTCTAATGATATCAGAAAAATTGCAGGAGCAATTAAGATTGGCAGAAGGACATTGAGGAAAATATACATAAACGTTGCCATCTCCATTGCCACAAAGGTTGTTATCTTTGCCCTCACTGCTGTAGGCTTGGTGTACCTCTGGATGGCAGTTCTGACAGATGTAGGGACCGGTATGCTTGTAACATTCAACAGTATGCTGCTCTTGAAAGGAGCAAAACGCTATGGTCATGTGGTCTGCAATCGCCAAGATTGTGATGATGGAGGTCACTGTCACAAGGATAATGATGGTGATGTTTATTACCATGTTCATGGTGGTGGCAACAGGCACCAGGTACACAGGAATATTGAGACATCAACATCAGCAGATTGCACTCATACAGTACAGGAGGCCGATACTTGTGGCGTTGTGAAATGCAGTGCATGCAATTTCCCGTCTTCGATTCATCATGGCTGCGCCAAGCCAAGGACCAGTCGCTGCTGTCAAAATTCTTATCCTCCTCAAGTTGATCTCTGTGCCTCCGGGAATTGCAGAGGCGGGAAAAGGCAGCTTGGAAGCAGTCACATCAACGAATCAAGATTCGAGCAACGCTGCAGTTCTTCCAATAACTGTTGTAAATCTGACAAGCTCGGAAACAGTCAAATGATCAGTGGTACTTCTTCCAGTAACTGCTGTAATTCTAGTAAACAGACAGGCGAATGTACTCAATCAAATGCTTATGAGGCAACAATAGAAGAATTGTTAGTTAGAAAGA

Fln Alignment:

sp_v1.1_unigene13581___KLVEEAQSQRSSTEEFVQKFAKYYTPVIVLTAMAMTLAPLIDHSLGF--RKWVYKA-----------LVVLVTACPCALVISTPVATACGLAHAAKTGLLIKGGRYLEALGKLKAIAFDKTGTLTEGIFRVVDVKFVDSETIDEHKWLSWIASLESRSSHPMSVAIVSYC------------------RQKGLEPSAEVKDFDILEGEGICGVVDNHKVYIGNVRLSNRLG------WTEYVPDTWDIRGATVGYFGTDEKFVGAFSVADNSRPEAAEALDKLKKLGIQLAMLTGDTAASAALIETQVGQIQVYSQLLPQDKVRIIGDLKSVG-KTAMIGXXXXXXXXXXXXXXXXXXXVAGSAVAMETSDIALMSNDIRKIAGAIKIGRRTLRKIYINVAISIATKVVIFALTAVGLVYLWMAV
B8LQ20_______________RLVETAQMAKAPIQKFADYIASVFVPVVVALAFVTWLGWYLAGVLGAYPEEWLPPASNHFVFALMFAISVLVIACPCALGLATPTAVMVATGIGATHGVLIKGGDALERAQKIQCVVFDKTGTLTKGKPSVTSAKVLSD--MQRGEFLRIVASAEASSEHPLARAVVDYAYHFHFFGEPPKDMDMQGLRTKNTAWLLTASKFEALPGKGVRCSIEGTEILVGNRKLMSEDGVFIPSVAEEYLKD-MEQHARTGILVAFDKELVGMLAISDPLKREAAVVIEGLKKMGILPIMVTGDNWTTARAVAQELGIEDVKAEVIPAGKAEVIRSLQKDGTMVAMVGDGINDSPALAAADVGMAIG-AGTDIAIEAADYVLMRNNLEDVITAIDLSKKTFARIRLNYVFAMGYNIFAIPL-AAGLFFPFLKI

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
1058	14		85		0.999981
1134	81		18		0.999994
1179		77		22	0.999999
1272	88		11		0.923564
1281	10			90	0.90298
1282		90		10	0.90298
1283	90		10		0.90298
1284		90	10		0.90298
1285	90		10		0.90298
1286	90		10		0.90298
1375			90	10	0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain