SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene13401

Length: 1395 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene13401 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Hydroxyethylthiazole kinase, putative (EC:2.7.1.50); K00878 hydroxyethylthiazole kinase [EC:2.7.1.50]	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	hydroxyethylthiazole kinase family protein	-	-	78%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:2.7.1.5-	-	0.0	78%

Source	ECs	Term	Type	e value	Identity
AutoFact		EC:2.7.1.5-	-	0.0	65%

Source	GOs	Term	Type	Identity
Blast2go	thiamine biosynthetic process	GO:0009228	Biological Process	78%
Blast2go	binding	GO:0005488	Molecular Function	78%
Blast2go	hydroxyethylthiazole kinase activity	GO:0004417	Molecular Function	78%
Blast2go	mitochondrion	GO:0005739	Cellular Component	78%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NPS1

Fln msg:

Fln protein: MEDLKEWKQNEIWGLEAWKSLCRLRELCPLIQCITNYVSMDIMANTLLAAGASPAMVHAVEEIADFTPHAKGLCINIGTLTPDWTVSMKAAAAKAAEAQNPWVLDPVAAGASKYRMNRCLELVDLKPTVIRGNASEILALAGASIGSAKGVDSSHVSTDALEAAKELACRTQAIVAVSGAVDLVTDGKRVLGVSNGVPLMQKITATGCAVTALIAAFVAIQPNLSLEATSFALAVFGIAGEIGMEKANGPASLRMHLIDALYGLDKESVLSKLKISEYH*

Protein Length: 281

Fln nts: AAGGAGGGGGTAAGGTGCTCGCGAGGACGAAACTGTTTTGAAACGCATATATTTGATGGGAATATATCTTTGTGGAAAAGAACTGCTTTGGATTCAGTGAAAAAATCTGCAGTTGGTAGATTGGAAGGGGTAAGATGCTTGGGTAAGGTGCTTGCGAGGAAGCCAGGAGCAAAGGCAAAGCCAGTAGAAAGCATAAGAATGGCGATCCGTGTTAGTCGAATTTCAGTGTAAGGGAAATCCCATAAGGACTTACAAAGGGAAGTGACATTCACAAAAAGTTGAGGTGACCTTTATGCA_-_ATGGAGGATTTGAAAGAGTGGAAACAGAATGAAATATGGGGGTTGGAGGCTTGGAAATCCCTGTGCAGGTTGAGGGAATTATGTCCTCTGATCCAGTGCATAACCAATTATGTGTCCATGGACATAATGGCTAATACCCTTCTGGCAGCAGGGGCTTCTCCGGCCATGGTTCATGCCGTTGAAGAGATTGCAGATTTTACTCCTCATGCTAAGGGCTTGTGCATCAATATTGGGACTCTGACCCCTGATTGGACTGTTTCAATGAAGGCTGCTGCAGCCAAGGCTGCAGAGGCTCAGAATCCTTGGGTATTGGATCCAGTGGCTGCAGGGGCTTCTAAATATAGAATGAATAGATGTCTGGAGCTTGTTGATCTTAAACCCACAGTTATACGAGGAAATGCTTCAGAAATCTTGGCATTAGCTGGTGCTTCTATTGGCTCAGCCAAGGGAGTAGACAGCAGCCATGTATCAACTGATGCCTTAGAAGCTGCAAAAGAATTGGCTTGTAGAACTCAAGCTATAGTTGCAGTATCGGGAGCTGTAGATCTTGTCACTGATGGCAAAAGAGTACTGGGAGTGAGCAATGGAGTGCCTTTGATGCAGAAGATCACTGCAACAGGTTGTGCAGTTACTGCACTTATTGCAGCTTTTGTTGCAATACAACCAAACCTTTCCTTGGAAGCAACTTCTTTCGCTCTTGCTGTCTTTGGGATCGCTGGCGAAATTGGGATGGAAAAAGCAAATGGACCGGCCTCATTGCGTATGCACTTGATAGATGCTTTATATGGTTTAGACAAGGAATCGGTACTATCAAAATTAAAGATTTCAGAGTATCATTAA___TTTTTGTAATTCTTACTGCAAACTTTCCCATGTAATTTCTGATAAAGGCTTCAACAGGCTTTCAAACATCAGCAACTTTTTGAAGCTCACCTCGTAATCCTATTGAATTTTAAAACAGAACATTGCATATTGAGGACCATTTCCCTTTGCTTGCTAAATCACGATAACTTTCAAAGATTTCGAAGCATGATTAATTTTTATATCTCTTACATCAAACTTATGTACATTCTGATAAAGGCTTTAACTGGTTTTCAAAAC

Fln Alignment:

sp_v1.1_unigene13401___MEDLKEWKQNEIWGLEAWKSLCRLRELCPLIQCITNYVSMDIMANTLLAAGASPAMVHAVEEIADFTPHAKGLCINIGTLTPDWTVSMXXXXXXXXXAQNPWVLDPVAAGASKYRMNRCLELVDLKPTVIRGNASEILALAGASIGSAKGVDSSHVSTDALEAAKELACRTQAIVAVSGAVDLVTDGKRVLGVSNGVPLMQKITATGCAVTALIAAFVAIQPNLSLEATSFALAVFGIAGEIGMEKANGPASLRMHLIDALYGLDKESVLSKLKISEY
A9NPS1_______________MEDLKEWKNNEIWGLEAWKSLCRVRELCPLIQCITNYVSMDIMANTLLAAGASPAMVHAVEEIADFTPHAKGLCINIGTLTPDWTVSMKAAAAKAAESQKPWVLDPVAAGASTYRMERCLELVDLKPTVIRGNASEILALAGASIGSTKGVDSSHVSTDALEAAKELACRTQAVVAVSGAVDLVTDGERVLGVSNGVPLMQKITATGCAVTALIAAFVALQPNLALEATCFALAVFGMAGEIGMEKANGPASLRMHLIDALYGLDKESVLSKINISKY

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
1174		16	66	16	0.988813
1175	50	16	16		0.988986
1176	83	16			0.994512
1177	66	16	16		0.988813
1178	33			50	1.0
1180		60	20	20	0.99255
1182	80	20			0.995493
1184	20	20		60	0.99255
1185	60		20		0.99255
1186	60	40			1.0
1189	20	60		20	0.99255
1195	25		75		0.996473
1197		75	25		0.996473
1199		25		75	0.996473
1200			25	75	0.996473
1296		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain