SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene11689

Length: 1159 nt

Origin of reads: Uagpf

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene11689

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Polygalacturonase precursor, putative (EC:3.2.1.67)	-	-	51%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	61%
Blast2go	protein	-	-	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Polygalacturonase.	EC:3.2.1.15	-	0.0	72%

Source	KEGGs	Term	Identity
Blast2go	Pentose and glucuronate interconversions	00040	72%
Blast2go	Starch and sucrose metabolism	00500	72%
Blast2go	Metabolic pathways	01100	72%

Source	GOs	Term	Type	Identity
Blast2go	carbohydrate metabolic process	GO:0005975	Biological Process	72%
Blast2go	cellular cell wall organization	GO:0007047	Biological Process	72%
Blast2go	polygalacturonase activity	GO:0004650	Molecular Function	72%
Blast2go	cell part	GO:0044464	Cellular Component	72%

Source	InterPros	Term	Type	Identity
Blast2go	Glycoside hydrolase, family 28	IPR000743	-	72%
Blast2go	Parallel beta-helix repeat	IPR006626	-	72%
Blast2go	Pectin lyase fold/virulence factor	IPR011050	-	72%
Blast2go	Pectin lyase fold	IPR012334	-	72%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PQB8

Fln msg: Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 271 - 440

Fln protein: RRDLGCHSLQEG*EKELQTSPCRSMTIDSSTHVIISNLKFQNSQQMHLVISGSTNVQVQNLQIVAPEDSPNTDGIHISTSQHVVVQNCKIGTGDDCVSIVSQSSDIKISKLACGPGHGISIGSLGKDNAKGTVSDVVVDGAYLTGTENGVRIKTWQGGSGYAKRIIFKNVIMNRVSNPIIIDQYYCDSRSPCQNQSSGVQVSDVTYTDISGTSATKEAIRLACSKSVPCQNIVMDNINLIHMDNGDPAIAFCENVHVLSRGRVDPPPLQR*

Protein Length: 272

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTGCAAGAGGGATAAGAGAAAGAGTTGCAGACAAGCCCCTGCCGGTCAATGACCATTGATTCTAGCACACATGTCATCATAAGCAATTTGAAGTTCCAGAACAGTCAACAGATGCATCTCGTAATATCTGGTTCAACAAATGTTCAAGTCCAAAACCTGCAAATCGTTGCACCGGAAGACAGTCCAAATACTGATGGAATTCACATATCTACCTCCCAACATGTAGTGGTTCAGAATTGTAAAATCGGAACAGGAGACGACTGTGTTTCTATTGTTTCACAGAGCTCAGATATTAAAATCAGCAAGCTAGCGTGCGGTCCTGGGCACGGAATAAGCATTGGAAGTCTGGGCAAGGATAATGCAAAAGGTACCGTTTCGGACGTGGTTGTTGATGGAGCTTATCTGACTGGCACAGAAAATGGAGTGCGTATAAAAACATGGCAGGGCGGTTCCGGATATGCCAAACGAATTATTTTCAAAAATGTGATAATGAATAGGGTCTCGAATCCCATCATTATAGACCAATATTACTGTGACTCTCGGAGTCCATGCCAAAATCAGAGTTCAGGGGTGCAAGTTAGCGACGTGACGTACACAGACATAAGCGGCACTTCGGCAACAAAAGAGGCAATAAGATTGGCGTGTAGCAAGAGTGTTCCATGTCAAAATATCGTAATGGACAACATAAATCTAATTCACATGGATAACGGAGATCCAGCCATTGCTTTCTGCGAGAATGTGCACGTTTTAAGCCGTGGTCGGGTCGACCCACCTCCTCTTCAGCGGTGA___TAAAATTATTTTCTTTGAATTTATTTGAATGGTAGATTTCAAAAATATGTTCCAGTTTACTGAGAAAAGCATGATAAATTGTTTCACTCTACCCTTATTTGTAGCCAAATATTTCTTGAAGCCCATTGCTCGATCCTGCTGCTATCTGTGAGATTCTGGCTTTATAGAAATAGTATGGTTGTGAAGGAAATCCTTAAATTTAACAACTATATTTGCTGGAATATTTGCAAGTTGGGGTTCTAGATATCAACACCCAGCCTTCTTCGTGGCCGTGTAATAATGGATCGGATCAGCTAATAACAATCCCACTATGATTGTTTGATGAGTTAATTAAATCTATTCTATT

Fln Alignment:

sp_v1.1_unigene11689___SMTIDSSTHVIISNLKFQNSQQMHLVISGSTNVQVQNLQIVAPEDSPNTDGIHISTSQHVVVQNCKIGTGDDCVSIVSQSSDIKISKLACGPGHGISIGSLGKDNAKGTVSDVVVDGAYLTGTENGVRIKTWQGGSGYAKRIIFKNVIMNRVSNPIIIDQYYCDSRS------PCQNQSSGVQVSDVTYTDISGTSATKEAIRLACSKSVPCQNIVMDNINLIHMDNGDPAIAFCENVHVLSRGRVDPP
C0PQB8_______________AFVVNSSSNVHLRDLTFQNSQKFHVVISLSKHVEVENLQIVAPGDSPNTDGIHIFTSEHVMIRNCTIATGDDCVSIIAQSSHIQVNDIICGPGHGISIGSLGKYNATDTVSDVVVNRASLSGTQNGLRIKTWQGGSGYAQGIIFQHVKMINVSNPIIIDQYYCAGSSRCNQPAPCNNQTSAVQVNNVTYTGITGTSATQEAIKLACSATVPCTNIVLEDISL-QLNNGDTPSSLSANVQGSSTGQVIPP

SNPs (tot: 94)

Position	A %	C %	G %	T %	Probability
53	60	40			1.0
55	60			40	1.0
57	60		40		1.0
58	60			40	1.0
60	40	60			1.0
63		60		40	1.0
66			40	60	1.0
73	60	40			1.0
78	40	60			1.0
114	25	12		62	1.0
115		25	75		1.0
116	62			37	1.0
117	62	37			1.0
118	25		75		1.0
120	12			87	0.992554
123	87			12	0.992554
124			87	12	0.992554
125	87		12		0.992554
126	87		12		0.992554
128	87			12	0.992554
135	87			12	0.992554
136		12	87		0.992554
277	81			18	0.999994
278		18	81		0.999994
280	81			18	0.999994
284		18	81		0.999994
285	81			18	0.999994
286		81		18	0.999994
287			18	81	0.999994
292		18		81	0.999994
293	18			81	0.999994
297		18		81	0.999994
298	18			81	0.999994
429		14		85	0.993533
431	85			14	0.993533
435	14		85		0.993533
437	85	14			0.993533
438		85		14	0.993533
439	85			14	0.993533
441	83		16		0.994512
442	83	16			0.994512
443	83			16	0.994512
444	83		16		0.994512
447		14	85		0.993533
448	87	12			0.992554
449		12	87		0.992554
451		12	75		0.975346
452	12	87			0.992554
456		12		87	0.992554
457	87	12			0.992554
458	87			12	0.992554
459	87			12	0.992554
460	87		12		0.992554
461	87		12		0.992554
466			87	12	0.992554
470		12	87		0.992554
471			87	12	0.992554
473	12		87		0.992554
474		11	88		0.923564
475			11	88	0.923564
478		88	11		0.923564
479			88	11	0.923564
482		11		88	0.923564
483	88			11	0.923564
486		88	11		0.923564
488	88			11	0.923564
489	88		11		0.923564
490	88			11	0.923564
494	87	12			0.992554
496	14			85	0.993533
518	75	25			1.0
519	75		25		1.0
521	75	25			1.0
524	25		75		1.0
525		25		75	1.0
528		75		25	1.0
530	75		25		1.0
531	75	25			1.0
533		75	25		1.0
534		75		25	1.0
567	30		60		1.0
569	90			10	0.90298
571	10			90	0.90298
574	90			10	0.90298
602	36		63		1.0
653			66	33	1.0
789	7			92	0.999602
790		92		7	0.999602
794	3	81	3	11	1.0
796	92		7		0.999602
797		88	3		0.998862
802	9			90	0.999842
928	37			62	1.0
1063	45		54		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain