SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene11465

Length: 563 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene11465
CGTCGTGACCTTGGCTGTCACTCATTCATTAGAATTTAAGAAAATGGTCAAGGCCTTGCATAGTGCAGGAATTGAGGTTATTTTGGATGTGGTTTACAACCATACAAATGAAGCGGATGATGAGCATCCTTTTACCACCTCATTTCGAGGTATAGATAATAAGGTTTATTACATGGTGGACACAAATAGCTATGTTCAATTGCTTAACTTTTCAGGATGTGGAAATACATTCAATTGTAACCATCCCGTGGTAATGGAATTAATATTGGATAGTCTAAGATATTGGGTCACAGAATATCATGTCAATGGATTCCGATTTGACCTTGCAAGTGTTCTATGTCGTGGACCTGATGGCACTCCTCTTAATGCTCCTCCTGTTATTAAGGCCATTGCAAAGGATCCAGTGCTTTCAAAATGCAAAATAATTGCAGAGCCATGGGACTGTGGGGGCCTATATTTAGTTGGCAGATTTCCCAATTGGGATAGGTGGGCTGAATGGAATGGCAAGTATCGAGATGATATTAGAAGGTTTATAAAGGGTGCTTCGCAGTGAGTGACAGGCC

Ace file of the UniGene sp_v1.1_unigene11465 (antisense)

If you push the Download ace button, you will download an ace file of this UniGene.

To watch this ACE file you will need an ACE viewer program as Tablet:

Go to the Tablet ACE viewer Web

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Isoamylase isoform 3 n=1 Tax=Solanum tuberosum RepID=Q84YG5_SOLTU	-	-	86%
FL-Next	sp=Isoamylase 3, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	85%
Blast2go	isoamylase isoform 3	-	-	90%

Source	ECs	Term	Type	e value	Identity
Blast2go	Alpha-amylase.	EC:3.2.1.1	-	0.0	90%

Source	KEGGs	Term	Type	Identity
Blast2go	Starch and sucrose metabolism	00500		90%
Blast2go	Metabolic pathways	01100		90%
Blast2go	Isoamylase.	EC:3.2.1.68	-	90%

Source	GOs	Term	Type	Identity
Blast2go	starch catabolic process	GO:0005983	Biological Process	90%
Blast2go	cation binding	GO:0043169	Molecular Function	90%
Blast2go	alpha-amylase activity	GO:0004556	Molecular Function	90%
Blast2go	isoamylase activity	GO:0019156	Molecular Function	90%
Blast2go	chloroplast starch grain	GO:0009569	Cellular Component	90%

Source	InterPros	Term	Type	Identity
Blast2go	Glycosyl hydrolase, family 13, catalytic domain	IPR006047	-	90%
Blast2go	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	90%
Blast2go	Alpha amylase	IPR015902	-	90%
Blast2go	Glycoside hydrolase, superfamily	IPR017853	-	90%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: sp_plants

Fln subject: Q9M0S5

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 256 aas, your sequence is shorter than subject: 184 - 764

Fln protein: VVTLAVTHSLEFKKMVKALHSAGIEVILDVVYNHTNEADDEHPFTTSFRGIDNKVYYMVDTNSYVQLLNFSGCGNTFNCNHPVVMELILDSLRYWVTEYHVNGFRFDLASVLCRGPDGTPLNAPPVIKAIAKDPVLSKCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDIRRFIKGASQ*

Protein Length: 185

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTCATTAGAATTTAAGAAAATGGTCAAGGCCTTGCATAGTGCAGGAATTGAGGTTATTTTGGATGTGGTTTACAACCATACAAATGAAGCGGATGATGAGCATCCTTTTACCACCTCATTTCGAGGTATAGATAATAAGGTTTATTACATGGTGGACACAAATAGCTATGTTCAATTGCTTAACTTTTCAGGATGTGGAAATACATTCAATTGTAACCATCCCGTGGTAATGGAATTAATATTGGATAGTCTAAGATATTGGGTCACAGAATATCATGTCAATGGATTCCGATTTGACCTTGCAAGTGTTCTATGTCGTGGACCTGATGGCACTCCTCTTAATGCTCCTCCTGTTATTAAGGCCATTGCAAAGGATCCAGTGCTTTCAAAATGCAAAATAATTGCAGAGCCATGGGACTGTGGGGGCCTATATTTAGTTGGCAGATTTCCCAATTGGGATAGGTGGGCTGAATGGAATGGCAAGTATCGAGATGATATTAGAAGGTTTATAAAGGGTGCTTCGCAGTGAGTGACAGGCC

Fln Alignment:

sp_v1.1_unigene11465___SLEFKKMVKALHSAGIEVILDVVYNHTNEADDEHPFTTSFRGIDNKVYYMVDTNSYVQLLNFSGCGNTFNCNHPVVMELILDSLRYWVTEYHVNGFRFDLASVLCRGPDGTPLNAPPVIKAIAKDPVLSKCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDIRRFIKGAS
Q9M0S5_______________SKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNN--QLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
409		90	10		0.90298
410	10			90	0.90298
411	90		10		0.90298
415	10	90			0.90298
416			10	90	0.90298
418		10	90		0.90298
421	90		10		0.90298
423		10	90		0.90298
425		10	90		0.90298
428		10	90		0.90298
435			88	11	0.923564
470	57		42		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain