SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene11023

Length: 2339 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene11023
CGTCGTGACCTTGGCTGTCACTCAAATTTTTTATTTTGTTCTTATGGGTAGAACGTAGTCGCAGGCAGCAGCAGAAGTAGAAGCTGGAAATCTCGATAGGAAGATGCATTTGAGCGAGAATGAAGGGATCGAGGGGAAAACTTTCGTTGTTACTGGAGGGTCAGGGTTTGTGGGAAGCGCGCTATGCTTGGAATTAATGCGACGCGGAGCTGAAGAGGTTAGAACGCTCGACCCGCGTGAAGATTCTCCCTGGACCCCTACTTTGCGCAGGAATGGCGTTGTCTGCATTAGAGGTGATGTAAGCAGAAGTCAGGATGTTGAGAAAGCTCTGAGAGGAGCAGATTGTGTTTTCCACCTTGCTTCATTTGGCATGTCAGGCAAAGAAATGCTTCAAGCAGGTCGCATTGATGAAGTAAATATAAATGGAACTTGCCTTGTACTGGATGCCTGTATCAAGTACGGTGTGAACAGGCTTGTATATACGAGCACATATAACGTTGTATATGGTGGACGTGAGATTGTAAATGGCAACGAAACATTGAGATATTTTCCAATGGAAGATCATGTTGATCCATATGGTCGGAGTAAAGCTCTTGCTGAACAGTTAGTTATCAAAAGCAATGCTCGTCCACTGAAAAAAAGAGATGTGAAAGAAGCTGTTTCTACGTGTGCGATCCGACCTGCAGCAATTTATGGGCCTGGTGAGGAGCGCCATCTTCCAAGAATACTCAGCTTAGCACAAATGGGCCTTCTAACTTTCAAAATTGGAGATGCTAGTGTGAAAAATGACTGGGTTTATGTTGACAACCTAGTACATGCCCTTTTGCTGGCAAGTATGGGACTTCTAGATGATATTCCAGGAAGAGAAGGAGTTCCTATTGCTGCCGGCCAAACATATTTTATCTCTGATGGTGCTCCAGTGAATACATTTGAGTTTCTCCGTCCATTAGTTGTGAGCTTAGAGTACGACTTTCCTAAGCTATCAATCAGTGTTCCCACTGCTCTTGTTGTGGCAAAGATCTTTTGGGTCATATATTTTCTCTTTTATCCTTGGTTGAAAAAGTCATGGTTTCCTTCACCGTTTATCCTACCCGCAGAAGTATACAAAGTGGGCATCACGCACTATTTCTCTATCTTAAAAGCCAAGCAGGAACTGGGATACACTCCTATAGTAAAACCTCAAGAAGGCATGAAAGCAACAATTACATATTGGAAAGAGAAGAAAAGGAAGGACCTGGACATGCCTAATATCTTTGCATGGTTGTTTTCTGTAGGTGGTATGTTTCAACTGTTCTGTTGTGCATTTATACCACCACTGTATCTTGGTCCCTTTGTGTGGGTAAGGTCATTTGGCCTTCTCGTATTTCAGTCTATAAAAAATCTGCAGATCCTCTTTTACATTTCAGCACTGCTCCATATTATAGAAGCTTGTTATGCATGGCTCTTGGCCCGAAGAGTAGATCCATCAAACGTAAAAGGATGGTTTTGGCAAACTTTTGCTCTGGGCTATTTCTCACTTCGTCTGCTTTTGAAAAGGGGAAAACACTAAACCAGGGGATATGCTTTCTTATCAGAAGTACTGTGACTTCTGGGCATTTATTGCATTGAAAGCATGAGTGCAATTTAAATTTACGACATAAGGTGGGTTTTTTCTTTAGTAATAATGAAGAGTGTTAAACTCTGCACTTCAGACGTTTTTCTTGCTGGGATTTCTAAAATCCATTACTTGAATATATGATACATAAATTTAATCGGCCTTTGTTTTAGTGCATGTACGATAAATGGACAATCAATTACTCACTGACGAAACTAGTGTTTGATTACTGTAGAGTCGAGGTACCCAATTACTGGACGCGACACTGATACAGTACATAAAAACCATTATGTGCTGGTTGCTCTCTGTGTGTGCTCATGCGGGCCTCCAAATATTTATCAAACTTCGTTCTGCGCCCACTGGCCTTACCATACTTATAGTTTCAGAAAAGTTGAATGAATCCAACTATACTTCTCAGTGGATCTGTGGGACTTGTAGAATGATGAGCAATACAAAACTTNCGTGGTTTGTTATTTCATTTTGCACCTAGAAAGTCCCAAAGCTTGATACCCAGTGTGTGCGTGTGGTCCAATTTAGGCACCTGTATTTCATCTTAAGAAAATAACATTGCTAAGCTCATAATCAAGTAGCCATCTAAAAGATTACATTGCGATCCCAAGTTTAAACAATAATATGTACATATAAAGCCATTAAATCATTCTTTAAATTTAGGAAACAGATACGCCATACATCTCCATTTCCTATTATAAATACTTGTGTACGCAAAAATCAGTTTGGAAAGACA

Ace file of the UniGene sp_v1.1_unigene11023 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Hydroxysteroid dehydrogenase, putative n=1 Tax=Ricinus communis RepID=B9R8T2_RICCO	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	92%
Blast2go	3-beta hydroxysteroid dehydrogenase isomerase family protein	-	-	75%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:1.1.1.17-	-	0.0	75%
Blast2go	3-beta-hydroxy-Delta(5)-steroid dehydrogenase.	EC:1.1.1.145	-	0.0	75%

Source	KEGGs	Term	Identity
Blast2go	Steroid hormone biosynthesis	00140	75%
Blast2go	Biosynthesis of terpenoids and steroids	01062	75%
Blast2go	Biosynthesis of plant hormones	01070	75%
Blast2go	Metabolic pathways	01100	75%

Source	GOs	Term	Type	Identity
Blast2go	steroid biosynthetic process	GO:0006694	Biological Process	75%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	75%
Blast2go	sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity	GO:0047012	Molecular Function	75%
Blast2go	binding	GO:0005488	Molecular Function	75%
Blast2go	3-beta-hydroxy-delta5-steroid dehydrogenase activity	GO:0003854	Molecular Function	75%
Blast2go	membrane	GO:0016020	Cellular Component	75%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	75%

Source	InterPros	Term	Type	e value	Identity
Blast2go	3-beta hydroxysteroid dehydrogenase/isomerase	IPR002225	-	0.0	75%
Blast2go	NAD(P)-binding domain	IPR016040	-	0.0	75%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AE01

Fln msg: your sequence is longer than subject: 482 - 304

Fln protein: MHLSENEGIEGKTFVVTGGSGFVGSALCLELMRRGAEEVRTLDPREDSPWTPTLRRNGVVCIRGDVSRSQDVEKALRGADCVFHLASFGMSGKEMLQAGRIDEVNINGTCLVLDACIKYGVNRLVYTSTYNVVYGGREIVNGNETLRYFPMEDHVDPYGRSKALAEQLVIKSNARPLKKRDVKEAVSTCAIRPAAIYGPGEERHLPRILSLAQMGLLTFKIGDASVKNDWVYVDNLVHALLLASMGLLDDIPGREGVPIAAGQTYFISDGAPVNTFEFLRPLVVSLEYDFPKLSISVPTALVVAKIFWVIYFLFYPWLKKSWFPSPFILPAEVYKVGITHYFSILKAKQELGYTPIVKPQEGMKATITYWKEKKRKDLDMPNIFAWLFSVGGMFQLFCCAFIPPLYLGPFVWVRSFGLLVFQSIKNLQILFYISALLHIIEACYAWLLARRVDPSNVKGWFWQTFALGYFSLRLLLKRGKH*

Protein Length: 483

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTTTTTATTTTGTTCTTATGGGTAGAACGTAGTCGCAGGCAGCAGCAGAAGTAGAAGCTGGAAATCTCGATAGGAAG_-_ATGCATTTGAGCGAGAATGAAGGGATCGAGGGGAAAACTTTCGTTGTTACTGGAGGGTCAGGGTTTGTGGGAAGCGCGCTATGCTTGGAATTAATGCGACGCGGAGCTGAAGAGGTTAGAACGCTCGACCCGCGTGAAGATTCTCCCTGGACCCCTACTTTGCGCAGGAATGGCGTTGTCTGCATTAGAGGTGATGTAAGCAGAAGTCAGGATGTTGAGAAAGCTCTGAGAGGAGCAGATTGTGTTTTCCACCTTGCTTCATTTGGCATGTCAGGCAAAGAAATGCTTCAAGCAGGTCGCATTGATGAAGTAAATATAAATGGAACTTGCCTTGTACTGGATGCCTGTATCAAGTACGGTGTGAACAGGCTTGTATATACGAGCACATATAACGTTGTATATGGTGGACGTGAGATTGTAAATGGCAACGAAACATTGAGATATTTTCCAATGGAAGATCATGTTGATCCATATGGTCGGAGTAAAGCTCTTGCTGAACAGTTAGTTATCAAAAGCAATGCTCGTCCACTGAAAAAAAGAGATGTGAAAGAAGCTGTTTCTACGTGTGCGATCCGACCTGCAGCAATTTATGGGCCTGGTGAGGAGCGCCATCTTCCAAGAATACTCAGCTTAGCACAAATGGGCCTTCTAACTTTCAAAATTGGAGATGCTAGTGTGAAAAATGACTGGGTTTATGTTGACAACCTAGTACATGCCCTTTTGCTGGCAAGTATGGGACTTCTAGATGATATTCCAGGAAGAGAAGGAGTTCCTATTGCTGCCGGCCAAACATATTTTATCTCTGATGGTGCTCCAGTGAATACATTTGAGTTTCTCCGTCCATTAGTTGTGAGCTTAGAGTACGACTTTCCTAAGCTATCAATCAGTGTTCCCACTGCTCTTGTTGTGGCAAAGATCTTTTGGGTCATATATTTTCTCTTTTATCCTTGGTTGAAAAAGTCATGGTTTCCTTCACCGTTTATCCTACCCGCAGAAGTATACAAAGTGGGCATCACGCACTATTTCTCTATCTTAAAAGCCAAGCAGGAACTGGGATACACTCCTATAGTAAAACCTCAAGAAGGCATGAAAGCAACAATTACATATTGGAAAGAGAAGAAAAGGAAGGACCTGGACATGCCTAATATCTTTGCATGGTTGTTTTCTGTAGGTGGTATGTTTCAACTGTTCTGTTGTGCATTTATACCACCACTGTATCTTGGTCCCTTTGTGTGGGTAAGGTCATTTGGCCTTCTCGTATTTCAGTCTATAAAAAATCTGCAGATCCTCTTTTACATTTCAGCACTGCTCCATATTATAGAAGCTTGTTATGCATGGCTCTTGGCCCGAAGAGTAGATCCATCAAACGTAAAAGGATGGTTTTGGCAAACTTTTGCTCTGGGCTATTTCTCACTTCGTCTGCTTTTGAAAAGGGGAAAACACTAA___ACCAGGGGATATGCTTTCTTATCAGAAGTACTGTGACTTCTGGGCATTTATTGCATTGAAAGCATGAGTGCAATTTAAATTTACGACATAAGGTGGGTTTTTTCTTTAGTAATAATGAAGAGTGTTAAACTCTGCACTTCAGACGTTTTTCTTGCTGGGATTTCTAAAATCCATTACTTGAATATATGATACATAAATTTAATCGGCCTTTGTTTTAGTGCATGTACGATAAATGGACAATCAATTACTCACTGACGAAACTAGTGTTTGATTACTGTAGAGTCGAGGTACCCAATTACTGGACGCGACACTGATACAGTACATAAAAACCATTATGTGCTGGTTGCTCTCTGTGTGTGCTCATGCGGGCCTCCAAATATTTATCAAACTTCGTTCTGCGCCCACTGGCCTTACCATACTTATAGTTTCAGAAAAGTTGAATGAATCCAACTATACTTCTCAGTGGATCTGTGGGACTTGTAGAATGATGAGCAATACAAAACTTtCGTGGTTTGTTATTTCATTTTGCACCTAGAAAGTCCCAAAGCTTGATACCCAGTGTGTGCGTGTGGTCCAATTTAGGCACCTGTATTTCATCTTAAGAAAATAACATTGCTAAGCTCATAATCAAGTAGCCATCTAAAAGATTACATTGCGATCCCAAGTTTAAACAATAATATGTACATATAAAGCCATTAAATCATTCTTTAAATTTAGGAAACAGATACGCCATACATCTCCATTTCCTATTATAAATACTTGTGTACGCAAAAATCAGTTTGGAAAGACA

Fln Alignment:

sp_v1.1_unigene11023___MRRGAEEVRTLDPREDSPWTPTLRRNGVVCIRGDVSRSQDVEKALRGADCVFHLASFGMSGKEMLQAGRIDEVNINGTCLVLDACIKYGVNRLVYTSTYNVVYGGREIVNGNETLRYFPMEDHVDPYGRSKALAEQLVIKSNARPLKKRDVKEAVSTCAIRPAAIYGPGEERHLPRILSLAQMGLLTFKIGDASVKNDWVYVDNLVHALLLASMGLLDDIPGREGVPIAAGQTYFISDGAPVNTFEFLRPLVVSLEYDFPKLSISVPTALVVAKIFWVIYFLFYPWLKKSWFPSPFILPAEVYKV
D5AE01_______________MRRGAEEVRTLDPREDSPWTTTLRRNGVVCIRGDVSQSKDVEKSLRGADCVFHLASYGMSGKEMLQAGRIDEVNINGSCLVLDACIKYGVNRLVYTSTYNVVYGGREIVNGNETLRYFPLEDHVDPYGRSKSLAEQLVLKSNARPLKKRDGKK-LYTCAIRPAAIYGPGEERHLPRIFSLAQMGLLTFKIGDPSVKNDWVYVDNLVHALLLASMGLLDDIPGREGIPIAAGQTYFISDGAPVNTFEFLRPLIISLDYDFPRLSISVPTALVVAKIFWAIYFLFYPWLKKSWFPSPLILPAEVYKV

SNPs (tot: 87)

Position	A %	C %	G %	T %	Probability
468		14		85	0.993533
500	16		83		0.994512
590	14			85	0.993533
643	83			16	0.994512
644	83		16		0.994512
646	83		16		0.994512
647		83		16	0.994512
649	16			83	0.994512
652	16			83	0.994512
657			16	83	0.994512
658			83	16	0.994512
677	80	20			0.995493
678		20		80	0.995493
679		20		80	0.995493
1024	87		12		0.999964
1026	87			12	0.999964
1027		87	12		0.999964
1030	12			87	0.999964
1031		12	87		0.999964
1032	12		87		0.999964
1033		14		85	0.999981
1034	14		85		0.999981
1037	85	14			0.999981
1038		14		85	0.999981
1041	85		14		0.999981
1042		2	8	88	1.0
1043			91	8	1.0
1047	87		12		1.0
1050	87			3	1.0
1061	93		6		0.999453
1066	93		6		0.999453
1067	6	93			0.999453
1070		92	7		0.999532
1072		7		92	0.999532
1077	92		7		0.999532
1078	92			7	0.999532
1079	92	7			0.999532
1082	92		7		0.999532
1085		92	7		0.999532
1090	92			7	0.999664
1091	92		7		0.999664
1096	92		7		0.999664
1097	7			92	0.999664
1098		7		92	0.999664
1101		8	92		0.999718
1104	8			92	0.999718
1106			8	92	0.999718
1107		91		8	0.999766
1110		8	91		0.999807
1112	8			91	0.999807
1114		91	8		0.999807
1147		13		86	0.999973
1148	13		86		0.999973
1151			13	86	0.999973
1206		18		81	0.999994
1211	90		10		0.90298
2013		72		27	1.0
2147		84		15	0.999986
2187		90		10	0.90298
2239			81	18	0.999994
2240		81	18		0.999994
2242		9	18	72	0.999994
2245			18	81	0.999994
2246	18	81			0.999994
2248	18			81	0.999994
2249	81		18		0.999994
2250		18		81	0.999994
2252	18		81		0.999994
2255	81		18		0.999994
2257		18		81	0.999994
2258	18			81	0.999994
2260		81	18		0.999994
2267		11		88	0.923564
2268	88	11			0.923564
2270	12			87	0.992554
2274	14		85		0.993533
2277			83	16	0.994512
2283	20			80	0.995493
2285	20	80			0.995493
2286	25		50	25	0.995533
2296	25			25	0.995533
2297			25	75	0.996473
2298	25	25	25	25	0.997389
2299		50	25	25	0.995533
2300		50	25	25	0.995533
2301	25	75			0.996473
2303	25			75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain