SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene10982

Length: 2162 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene10982
ATGGGATCACTTTACCTGCTCTAGTAATAGCAGTTCTCAGAGTCTTTAAGAATCAGCTCTGTTAAGCTGCCCATAGATCTGTCGAAAATGTTTAGTCTCGCTTTTACGTGTTCTTCCAATTCTAGCAATAACATCCTCTGTCGCTCTGAATGTTTTGCGGAGCAAGTACACGGTCCGAAGATATGCCTGAAAATCCCAGGAAAGAAGTTTGTCGAATGAATACCATGAAGCTGCCTTCTTTGAGAATTTCACAGCAGCAGAGGACCGGGTTGTGTAAGGCCTACAGTGTGCAAACCCAGACTGTTGTTGAGAAAGAGTTAATGCACGAAAGCCTTGCAATTAGTGAGGTACCGATAAAAGGAGTGCCGGTTATTGTGATGCTGCCTCTGGATACTGTGAAACCCAATGGGACCCTTAATCGAAAGCGCGCCCTGAATGCTAGCCTCATGGCGCTCAAAAGTGGAGGAGTGAAAGACGTAATGGTGGAGGTGTGGTGGGGCCTGGTGGAGAAAGATGGCCCTGGTAACTACAACTGGTCCGGATATAAGGAGATCGTTGAAATGGTTAGCAAGCATGGGTTGAAGCTTCAGGCAGTAATGTCTTTCCATCAATGTGGAGGAAACGTTGGCGACACTTGCAATATTCCCTTACCTCGCTGGGCTTTGGAAGAAGTCGAGAGTAATCCAGATTTGGTATACACCGATAAGGCTGGAAGGAGAAACAAAGAGTATATCTCGTTAGGATGCGACACCCTGCCCGTGCTTAAGGGAAGAACGCCGATTCAGGTATACGCAGATTATATGAGAAGTTTCAGAGATACGTTCAGAGATATGCTCGGAAAAGTCATAGTGGAAATTCAAGTTGGAATGGGGCCTTGTGGGGAACTGAGATATCCCTCGTATCCTGAAAGCAACGGCACCTGGCGTTTCCCTGGGATTGGCGAATTTCAGTGCTATGACAAGTACATGAAGGCGTCCTTAAGAGCCCGTGCAGAGGCCGTTGGGAATGGCGAGTGGGGGCTAGACTGCCCTCGGGATTCAGGGAATTATAACCAGTTTCCGGAAGACACTGGCTTCTTTCGGAGAGAAGGAACATGGGACACCGATTATGGCAGATTCTTTCTCAATTGGTATTCGGAAATGTTACTTGCTCATGGTGACAGAATTCTGAGTGCTGCAGAAGGCGTTTTCAGGGGCAGCGGTGCCAAATTATCTGGAAAAGTTGCAGGGATCCACTGGCACTACGGCTCAAAATCTCACGCTGCAGAGCTGACTGCGGGATACTATAACACCAGAGAAAGCGATGGGTATCTCCCCATTGCACGCATGTTTAAGAAACACCGAACTGTTCTGAACTTCACGTGCTTCGAGATGAAAGATGATGAGCAGCCACAGCACGCACGGTGCAGTCCGGAGGGGCTTCTGAGACAGGTTACGGAGGCTACCAGGAAAACAGGTGTGGGCCTTGCTGGGGAGAATGCGTTGCAGAGGTTCGATGGAAGGGCATACGCCCAGATCATGCACAATTCAAATCTCAAGCCGCGTGATAGTAATGATAGCAAGTCCCAGATGTGTGCCTTCACGTATCTGAGGATGAACCAGCAAATGTTTCAGAGCGAGAATTGGCATCGCTTCGTGTGGTTTGTAAGGAACATGAGCGAGGGACGAACGCTGAGGCATGGAGAAGAGGATCGTTCTGAAACGGAGCTCAAGTTCAATGCCGCTGCGTATCTGAGAAATGATGCCGCAGCTCTGATGCACGCTTAGGTTTATTATTGATTCTGTCTACCAAAGTGTGTAATTAAGAGGGTGTATTATATGGTATCGGGATAAGTTGTGCTTATCAATAGGTTCGTGTGCAGTATGCTGAGAATAAAATGTCCGAGCATAGTTTTATTAGTCAAGCGATTCAGCCTCATTTCCACTCTAAAATGTCCAGAGCATAGTTTTATTAGTCAAGCGATTCAGCCTCATTTCCATTAGATGTGAGGTATTTATAGCAATGGAATCCTCTACATACTGTATAAAGATTTATGAGTAGAATACTCAATACTTTCTATAACATTCTTCCCATTTGTAATGTCGACTTTATATCATCAATAAAAGGTAATTGTAAATTGATATTTATCTGACATATAAAAAGTTCTGCATTTATCATGTGTG

Ace file of the UniGene sp_v1.1_unigene10982 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	beta-amylase (EC:3.2.1.2)	-	-	70%
FL-Next	sp=Beta-amylase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	62%
Blast2go	protein	-	-	78%

Source	ECs	Term	Type	e value	Identity
Blast2go	Beta-amylase.	EC:3.2.1.2	-	0.0	78%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Starch and sucrose metabolism	00500		0.0	78%
Blast2go	Metabolic pathways	01100		0.0	78%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	78%
Blast2go	maltose biosynthetic process	GO:0000024	Biological Process	78%
Blast2go	starch catabolic process	GO:0005983	Biological Process	78%
Blast2go	cation binding	GO:0043169	Molecular Function	78%
Blast2go	beta-amylase activity	GO:0016161	Molecular Function	78%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	78%

Source	InterPros	Term	Type	Identity
Blast2go	Glycoside hydrolase, family 14B, plant	IPR001371	-	78%
Blast2go	Glycoside hydrolase, family 14	IPR001554	-	78%
Blast2go	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	78%
Blast2go	Glycoside hydrolase, superfamily	IPR017853	-	78%
Blast2go	Glycoside hydrolase, family 14, conserved site	IPR018238	-	78%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NVE4

Fln msg: your sequence is longer than subject: 539 - 492

Fln protein: MFCGASTRSEDMPENPRKEVCRMNTMKLPSLRISQQQRTGLCKAYSVQTQTVVEKELMHESLAISEVPIKGVPVIVMLPLDTVKPNGTLNRKRALNASLMALKSGGVKDVMVEVWWGLVEKDGPGNYNWSGYKEIVEMVSKHGLKLQAVMSFHQCGGNVGDTCNIPLPRWALEEVESNPDLVYTDKAGRRNKEYISLGCDTLPVLKGRTPIQVYADYMRSFRDTFRDMLGKVIVEIQVGMGPCGELRYPSYPESNGTWRFPGIGEFQCYDKYMKASLRARAEAVGNGEWGLDCPRDSGNYNQFPEDTGFFRREGTWDTDYGRFFLNWYSEMLLAHGDRILSAAEGVFRGSGAKLSGKVAGIHWHYGSKSHAAELTAGYYNTRESDGYLPIARMFKKHRTVLNFTCFEMKDDEQPQHARCSPEGLLRQVTEATRKTGVGLAGENALQRFDGRAYAQIMHNSNLKPRDSNDSKSQMCAFTYLRMNQQMFQSENWHRFVWFVRNMSEGRTLRHGEEDRSETELKFNAAAYLRNDAAALMHA*

Protein Length: 540

Fln nts: ATGGGATCACTTTACCTGCTCTAGTAATAGCAGTTCTCAGAGTCTTTAAGAATCAGCTCTGTTAAGCTGCCCATAGATCTGTCGAAAATGTTTAGTCTCGCTTTTACGTGTTCTTCCAATTCTAGCAATAACATCCTCTGTCGCTCTGA_-_ATGTTTTGCGGAGCAAGTACACGGTCCGAAGATATGCCTGAAAATCCCAGGAAAGAAGTTTGTCGAATGAATACCATGAAGCTGCCTTCTTTGAGAATTTCACAGCAGCAGAGGACCGGGTTGTGTAAGGCCTACAGTGTGCAAACCCAGACTGTTGTTGAGAAAGAGTTAATGCACGAAAGCCTTGCAATTAGTGAGGTACCGATAAAAGGAGTGCCGGTTATTGTGATGCTGCCTCTGGATACTGTGAAACCCAATGGGACCCTTAATCGAAAGCGCGCCCTGAATGCTAGCCTCATGGCGCTCAAAAGTGGAGGAGTGAAAGACGTAATGGTGGAGGTGTGGTGGGGCCTGGTGGAGAAAGATGGCCCTGGTAACTACAACTGGTCCGGATATAAGGAGATCGTTGAAATGGTTAGCAAGCATGGGTTGAAGCTTCAGGCAGTAATGTCTTTCCATCAATGTGGAGGAAACGTTGGCGACACTTGCAATATTCCCTTACCTCGCTGGGCTTTGGAAGAAGTCGAGAGTAATCCAGATTTGGTATACACCGATAAGGCTGGAAGGAGAAACAAAGAGTATATCTCGTTAGGATGCGACACCCTGCCCGTGCTTAAGGGAAGAACGCCGATTCAGGTATACGCAGATTATATGAGAAGTTTCAGAGATACGTTCAGAGATATGCTCGGAAAAGTCATAGTGGAAATTCAAGTTGGAATGGGGCCTTGTGGGGAACTGAGATATCCCTCGTATCCTGAAAGCAACGGCACCTGGCGTTTCCCTGGGATTGGCGAATTTCAGTGCTATGACAAGTACATGAAGGCGTCCTTAAGAGCCCGTGCAGAGGCCGTTGGGAATGGCGAGTGGGGGCTAGACTGCCCTCGGGATTCAGGGAATTATAACCAGTTTCCGGAAGACACTGGCTTCTTTCGGAGAGAAGGAACATGGGACACCGATTATGGCAGATTCTTTCTCAATTGGTATTCGGAAATGTTACTTGCTCATGGTGACAGAATTCTGAGTGCTGCAGAAGGCGTTTTCAGGGGCAGCGGTGCCAAATTATCTGGAAAAGTTGCAGGGATCCACTGGCACTACGGCTCAAAATCTCACGCTGCAGAGCTGACTGCGGGATACTATAACACCAGAGAAAGCGATGGGTATCTCCCCATTGCACGCATGTTTAAGAAACACCGAACTGTTCTGAACTTCACGTGCTTCGAGATGAAAGATGATGAGCAGCCACAGCACGCACGGTGCAGTCCGGAGGGGCTTCTGAGACAGGTTACGGAGGCTACCAGGAAAACAGGTGTGGGCCTTGCTGGGGAGAATGCGTTGCAGAGGTTCGATGGAAGGGCATACGCCCAGATCATGCACAATTCAAATCTCAAGCCGCGTGATAGTAATGATAGCAAGTCCCAGATGTGTGCCTTCACGTATCTGAGGATGAACCAGCAAATGTTTCAGAGCGAGAATTGGCATCGCTTCGTGTGGTTTGTAAGGAACATGAGCGAGGGACGAACGCTGAGGCATGGAGAAGAGGATCGTTCTGAAACGGAGCTCAAGTTCAATGCCGCTGCGTATCTGAGAAATGATGCCGCAGCTCTGATGCACGCTTAG___GTTTATTATTGATTCTGTCTACCAAAGTGTGTAATTAAGAGGGTGTATTATATGGTATCGGGATAAGTTGTGCTTATCAATAGGTTCGTGTGCAGTATGCTGAGAATAAAATGTCCGAGCATAGTTTTATTAGTCAAGCGATTCAGCCTCATTTCCACTCTAAAATGTCCAGAGCATAGTTTTATTAGTCAAGCGATTCAGCCTCATTTCCATTAGATGTGAGGTATTTATAGCAATGGAATCCTCTACATACTGTATAAAGATTTATGAGTAGAATACTCAATACTTTCTATAACATTCTTCCCATTTGTAATGTCGACTTTATATCATCAATAAAAGGTAATTGTAAATTGATATTTATCTGACATATAAAAAGTTCTGCATTTATCATGTGTG

Fln Alignment:

sp_v1.1_unigene10982___GVPVIVMLPLDTVKPNGTLNRKRALNASLMALKSGGVKDVMVEVWWGLVEKDGPGNYNWSGYKEIVEMVSKHGLKLQAVMSFHQCGGNVGDTCNIPLPRWALEEVESNPDLVYTDKAGRRNKEYISLGCDTLPVLKGRTPIQVYADYMRSFRDTFRDMLGKVIVEIQVGMGPCGELRYPSYPESNGTWRFPGIGEFQCYDKYMKASLRARAEAVGNGEWGLDCPRDSGNYNQFPEDTGFFRREGTWDTDYGRFFLNWYSEMLLAHGDRILSAAEGVFRGSGAKLSGKVAGIHWHYGSKSHAAELTAGYYNTRESDGYLPIARMFKKHRTVLNFTCFEMKDDEQPQHARCSPEGLLRQVTEATRKTGVGLAGENALQRFDGRAYAQIMHNSNLKPRDSN--DSKSQMCAFTYLRMNQQMFQSENWHRFVWFVRNMSEGRTLRHGEEDR
A9NVE4_______________GVPVFVKLPLDSVTSKHTVNRRKAMDASLMALKSAGVEGVMVNVWWGLVEREAPGEYNWSAYRELLEMVRKRGLKVQAVMSFHKCGGNVGDSVSIPLPKWVVEEIDRDNDLAYTDQWERRNYEYISLGCDNLPVLKGRTPVQCYSDFMRSFKENFTDLMGETVVEIQVGMGPAGELRYPSYPESNGTWKFPGIGAFQCYDKYMLANLKATAETAGKKEWGCGGPTNAGYYNNWSEETEFFCSEGGWNSPYGEFFLQWYSNMLLNHGERILAEAESIFHKSGARLSGKVAGIHWHYLTRSHAPELTAGYYNTGDRDGYLPIAQMFGRHGVVFIFTCIEMKDVEQPADAKCSPEKLIKQVIKATRKARIHLAGENALPRFDEAAYTQVLNNSCLRLEQDNPDDKIEPMCAFTYLRMSQHLFQSKNWSTFVSFVRRMSQQNAVSISRDEK

SNPs (tot: 44)

Position	A %	C %	G %	T %	Probability
42	75			25	0.996473
202		14	85		0.993533
210		85		14	0.993533
261		83		16	0.994512
405		25		75	0.996473
508		25		75	0.996473
624		22		77	0.999999
669	16		83		0.994512
672	16		83		0.994512
690	20	80			0.995493
1485	77		22		0.999999
1486		88		11	0.923564
1541		85	14		0.993533
1542		14	71	14	0.984337
1543		14		85	0.993533
1544	14			85	0.993533
1546		83	16		0.994512
1547	16	83			0.994512
1548			16	83	0.994512
1549		83		16	0.994512
1550		83	16		0.994512
1554			20	80	0.995493
1555		20		80	0.995493
1556		20	80		0.995493
1558	20			80	0.995493
1561	80	20			0.995493
1563	83	16			0.994512
1567	83		16		0.994512
1572	20			80	0.995493
1613			50	50	1.0
1625		75		25	0.996473
1626	25		75		0.996473
1632	25		75		0.996473
1665	75		25		0.996473
1673	80	20			0.995493
1676		80		20	0.995493
1689	25		75		0.996473
1832		50	50		1.0
1838		33	66		0.997452
1930		33	66		0.997452
1939	66	33			0.997452
1940		33		66	0.997452
1941		33	66		0.997452
1942	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain